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Search Results: 1 - 10 of 21753 matches for " Mahesh Kumar Sah "
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The extraction of fibroin protein from Bombyx mori silk cocoon: Optimization of process parameters
Mahesh Kumar Sah,Arvind Kumar,Pramanik K.
International Journal of Bioinformatics Research , 2010,
Abstract: Optimization of protein extraction using silk cocoon was carried out by the design of experiment(DOE) to obtain the response surface methodology ( RSM ). Box-Behnken rotatable design was used tocreate an experimental program to provide data to model the effects of various factors on protein extractionefficiency. The variables chosen were sodium carbonate concentration ( 1 X ), Lithum bromide concentration( 2 X ) and temperature ( 3 X ). The mathematical relationship between protein extraction efficiency and threesignificant independent variables can be approximated by a second order quadratic model. RSM wasused to describe the individual and interactive effects of three variables at three levels, combined accordingto a Box-Behnken Design. The coefficient of determination (2 R ) for the model is 0.9761. Probability value( P < 0.0001) demonstrates a very high significance for the regression model indicating that Box-BehnkenDesign can be applied to the protein extraction from silk cocoon, and it is an economical way of obtainingthe maximum amount of information with the fewest experiments.
Rapid and Reliable Method of High-Quality RNA Extraction from Diverse Plants  [PDF]
Saroj Kumar Sah, Gurwinder Kaur, Amandeep Kaur
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.521329
Abstract: The isolation of high quality RNA is a crucial technique in plant molecular biology. The quality of RNA determines the reliability of downstream process like real time PCR. In this paper, we reported a high quality RNA extraction protocol for a variety of plant species. Our protocol is time effective than traditional RNA extraction methods. The method takes only an hour to complete the procedure. Spectral measurement and electrophoresis were used to demonstrate RNA quality and quantity. The extracted RNA was further used for cDNA synthesis, expression analysis and copy number determination through Real Time PCR. The results indicate that RNA was of good quality and fit for real time PCR. This high throughput plant RNA extraction protocol can be used to isolate high quality RNA from diverse plants for real time PCR and other downstream applications.
Risk Map of Cholera Infection for Vaccine Deployment: The Eastern Kolkata Case
Young Ae You, Mohammad Ali, Suman Kanungo, Binod Sah, Byomkesh Manna, Mahesh Puri, G. Balakrish Nair, Sujit Kumar Bhattacharya, Matteo Convertino, Jacqueline L. Deen, Anna Lena Lopez, Thomas F. Wierzba, John Clemens, Dipika Sur
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071173
Abstract: Background Despite advancement of our knowledge, cholera remains a public health concern. During March-April 2010, a large cholera outbreak afflicted the eastern part of Kolkata, India. The quantification of importance of socio-environmental factors in the risk of cholera, and the calculation of the risk is fundamental for deploying vaccination strategies. Here we investigate socio-environmental characteristics between high and low risk areas as well as the potential impact of vaccination on the spatial occurrence of the disease. Methods and Findings The study area comprised three wards of Kolkata Municipal Corporation. A mass cholera vaccination campaign was conducted in mid-2006 as the part of a clinical trial. Cholera cases and data of the trial to identify high risk areas for cholera were analyzed. We used a generalized additive model (GAM) to detect risk areas, and to evaluate the importance of socio-environmental characteristics between high and low risk areas. During the one-year pre-vaccination and two-year post-vaccination periods, 95 and 183 cholera cases were detected in 111,882 and 121,827 study participants, respectively. The GAM model predicts that high risk areas in the west part of the study area where the outbreak largely occurred. High risk areas in both periods were characterized by poor people, use of unsafe water, and proximity to canals used as the main drainage for rain and waste water. Cholera vaccine uptake was significantly lower in the high risk areas compared to low risk areas. Conclusion The study shows that even a parsimonious model like GAM predicts high risk areas where cholera outbreaks largely occurred. This is useful for indicating where interventions would be effective in controlling the disease risk. Data showed that vaccination decreased the risk of infection. Overall, the GAM-based risk map is useful for policymakers, especially those from countries where cholera remains to be endemic with periodic outbreaks.
Change in Energy of Non-Spinning Black Holes w.r.t. the Change in Mass  [PDF]
Dipo Mahto, Rama Nand Mehta, Neeraj Pant, Raj Kumar Sah
International Journal of Astronomy and Astrophysics (IJAA) , 2013, DOI: 10.4236/ijaa.2013.31006

The present work discusses the derivation of the formula for the change in energy of non-spinning black holes with respect to the change in mass (dE/dM), which gives a constant quantity equal to 8.9998 x 1016 Joule/kg in both categories of X-ray binaries (XRBs) and Active Galactic Nuclei (AGN). This formula can be used to justify the life time of black hole given by Γ = 2.098(M/Mο)3 x 1067 years as proposed by Stephen Hawking, where M and Mο are the mass of the black hole and the sun respectively. The authors also calculate the change in energy and mass of non-spinning black holes with respect to the change in the radius of event horizon as well as (dE/dM) for different test non-spinning black holes in X-ray binaries (XRBs) and Active Galactic Nuclei (AGN).

Reliable and Efficient Routing Using Adaptive Genetic Algorithm in Packet Switched Networks
Rakesh Kumar,Mahesh Kumar
International Journal of Computer Science Issues , 2012,
Abstract: To identify the optimal route is a complex task in packet switched network because optimization depends upon a number of parameters. In this paper Genetic Algorithm is used to locate the optimal route. Genetic Algorithm starts with a number of solutions where each solution is represented in the form of chromosome using the permutation encoding scheme. The success of Genetic Algorithm depends upon the number of operators such as selection, mutation and crossover. Needless to say crossover is most innovative. In this paper crossover operators proposed namely 1-point, 2-point, and adaptive, have been customized according to the need of computer network. The fitness of each solution is evaluated in terms of historical reliability factor, node success/failure and delay. The performance of the proposed approach has been compared with Dijkstra Algorithm and improvement has been observed.
Towards Expeditious and Unswerving Routing to Corroborate Nascent Internet
Shishir Kumar,Mahesh Kumar
Computer Science , 2009,
Abstract: The internet is now-a-days experiencing a stress due to some inherent problems with the main interdomain routing protocol, boarder gateway protocol (BGP), the amount of time it takes to converge, number of update message exchanged followed by a failure to stabilize, the amount of time required to get a valid alternate path following the failure, the way size of routing table increasing, and security issues like integrity and privacy of routing tables and routing updates exchanged among the routers, are of our primary concern. In our proposed research work we plan to address aforementioned issues related to internet routing specially in boarder gateway protocol to enable BGP to offer expeditious unswerving routing to corroborate nascent internet. We plan to make some changes in the design of boarder gateway protocol and may introduce addition of extra features in BGP to help support above mentioned objective.
Catching Of Stolen Vehicles With Unique Identification Code Using Embedded Systems
Mahesh Kumar Shakya
International Journal of Electronics Communication and Computer Technology , 2012,
Abstract: The main purpose of this concept is to catch the stolen vehicles by a latest technology. This research work is developing a smart logic to identify the stolen vehicle on check post or Toll base. Still there is no technique to identify the vehicle on check posts by any mean. To employ this technology in use, we issue one unique identification code to every vehicle. This unique number is stored in the silicon chip and the chip is installed in the vehicle. No one can change this number because this UID chip is installed in the engine of the vehicle. This vehicle number is not available in the market. Now the vehicle is equipped with the UID code. This code is also stored in the Data base of check post or Toll base. Now when any vehicle passed through the check post/Toll then at the check post/Toll RF passive vehicle reader generates a 125 kHz frequency for decoding RF tag( which has been installed in the vehicle). If the data base does not find the stolen UID code then security gate gets OPEN and if the stolen UID code is matched with the data base then security gate remains closed and alarm becomes ON automatically and finally the stolen vehicle is caught
Spectrum of Lesions in Cystoscopic Bladder Biopsies -A Histopathological Study
Mahesh Kumar U
Al Ameen Journal of Medical Sciences , 2012,
Abstract: Background: Diseases of the bladder, particularly inflammation (cystitis), constitute an important source of clinical signs and symptoms. Tumors of the bladder are an important source of both morbidity and mortality. Objectives: 1) To study the histopathological features of various lesions in bladder biopsies. 2) To study the frequency of different pathological lesions, particularly Transitional Cell Carcinoma (TCC) in urinary bladder biopsies. Results: Sixty cases of urinary bladder biopsies were received. Out of 60 patients, 43 were males and 17 were females. The spectrum of pathological lesions included inflammations, metaplastic lesions and tumours. Transitional cell carcinoma was the most common tumour seen in this study. A case of paraganglioma and malakoplakia were also seen. Conclusions: Our study has revealed that the bladder tumours are the commonest lesions in cystoscopic biopsies and TCC was the predominant tumour type
Language of Saptariya Tharu
Mahesh Kumar Chaudhary
Researcher: A Research Journal of Culture and Society , 2013, DOI: 10.3126/researcher.v1i2.9885
Abstract: The purpose of this paper is to present a general overview of the Tharu language as it is spoken in Saptari District. Geographical distribution of Tharu, its genetic affiliation, linguistics characteristics; phonology, morphology and syntax are looked into brief. This paper is based mainly on primary sources along with secondary one. DOI: http://dx.doi.org/10.3126/researcher.v1i2.9885 Researcher 1(2) 2013: 49-62
Approach to Meniscal Tear by Clinical Examination and Its Management: A Review  [PDF]
Roshan Sah, Luike Bin, Vijay Kumar Sah, Keshav Singh Dhami
Yangtze Medicine (YM) , 2019, DOI: 10.4236/ym.2019.33020
Abstract: It’s quite difficult to diagnose the meniscus tear by clinical examination even the experienced orthopaedic surgeon, so this review has shortly focused on clinical examination and its treatment. How to overcome missed diagnosis of meniscus tear in clinical setting? This review article highlighted the importance of early diagnosis and treatment; fortunately, these processes have been vastly improved by advances in Magnetic resonance imaging (MRI) and Arthroscopy. Although partial, subtotal and total meniscectomy is the choice for different types of meniscal tears, there are certain criteria to be meet for undergoing surgical treatment. The most common criteria for meniscal repair include: 1) A vertical longitudinal tear more than 1 cm in length located within vascular zone, 2) A tear which is unstable and displaceable into the joint, 3) An active informed and cooperative patient but younger than 40 years old, 4) A stable knee or wound stabilized with a ligamentous reconstruction. Chronically deformed or degenerative menisci are bottom-out candidates for repair. Most investigators report that only 10% to 15% of meniscal tears can be repaired and that most such repairs are done in association with an anterior cruciate ligament reconstruction. However, this article presents a review of the clinical relevant anatomic, function, repair and healing mechanism, description of the meniscus attachment as well as current strategies for accurate diagnosis and treatment of common injuries to these meniscus attachments.
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