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Search Results: 1 - 10 of 145232 matches for " Magali F Grando "
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Developmental changes in abundance of the VSPβ protein following nuclear transformation of maize with the Soybean vspβ cDNA
Magali F Grando, Rex L Smith, Cristina Moreira, Brian T Scully, Robert G Shatters
BMC Plant Biology , 2005, DOI: 10.1186/1471-2229-5-3
Abstract: From 81 bombardments, 101 plants were regenerated, and plants from five independent lines produced vspB transcripts and VSPβ polypeptides. In leaves from seven-week-old plants (prior to flowering), VSPβ accumulated to 0.5% of the soluble leaf protein in primary transgenic plants (R0), but to only 0.03% in R1 plants. During seed-filling (silage-stage) in R1 plants, the VSPβ protein was no longer detected in leaves and stems despite continued presence of the vspB RNA. The RNA transcripts for this peptide either became less efficiently translated, or the VSPβ protein became unstable during seed-fill.Developmental differences in the accumulation of soybean VSPβ when transgenically expressed in maize show that despite no changes in the vspB transcript level, VSPβ protein that is readily detected in leaves of preflowering plants, becomes undetectable as seeds begin to develop.Although genetic variation for protein content has been found in forage plants, this variability is narrower than that observed for other traits such as digestibility [1]. Since the major protein components in monocot forage and silage crops are involved in metabolic activity, and hence are not "true" storage proteins, it has been argued that it is not feasible to make major changes in protein quality or protein composition by conventional breeding [1]. However, genetic engineering may allow improvement in protein quality and content through expression of a storage protein not found in grass vegetative tissue.Genes encoding seed storage proteins of various plant species have been transgenically expressed to test for improvement of nutritional quality. Most experiments were conducted with tobacco and legume species including alfalfa, soybean, canola, clover and lupins. For nuclear-targeted genes, accumulation of these seed storage proteins in vegetative tissue of transgenic plants was either undetectable or very low. These included pea vicilin [2,3], soybean conglycinin [4], sunflower seed agglutinin
Proposal for commercial classification of the gerbera capitulum based in the flower overlap index
Cardoso, Raquel DL;Scheffer-Basso, Simone M;Grando, Magali F;
Horticultura Brasileira , 2010, DOI: 10.1590/S0102-05362010000200013
Abstract: the aim of this work was to propose a methodology for commercial classification of gerbera′s capitulum based on the trans flowers overlap coefficient. three to eight capitula of twenty-eight cultivars of gerbera cut flower and seven non-commercial accesses were used. the width measurement of the trans flowers group (internal ray flower) as well as the sum of the width of the trans and ray flowers (external ray flower) groups were taken from each capitulum. the average of each access and the overlap coefficient were calculated (cs = width of the trans flowers group / σ of the width of the trans and ray flowers groups) and submitted to the multivariate analysis, to verify the grouping by the average euclidian distance matrix. it is proposed that gerberas with cs= 0.01 to 0.15 be classified as simple, cs= 0.16 to 0.40 as semi-double and cs superior to 0.41 as double. the overlap coefficient (cs) can be used to systematize the classification of the gerbera capitula, reducing the divergences in the classification and the subjectivity in the decisions of producers, florists and breeders.
Germina??o in vitro de sementes de alcachofra
Moraes, Cassieli F de;Suzin, Marilei;Nienow, Alexandre Augusto;Grando, Magali F;Mantovani, Nilton;Calvete, Eunice O;Donida, Beatriz Terezinha;
Horticultura Brasileira , 2010, DOI: 10.1590/S0102-05362010000100012
Abstract: low multiplication rates and high contamination in the explants are some of the difficulties in artichoke micropropagation. in vitro seed germination may be an alternative to obtain healthy explants for use in future in vitro cultivation. this project developed at the laboratory of universidade de passo fundo was established to evaluate cv. 'nobre' artichoke seeds in vitro germination. in three experiments, active chloride concentrations on seed aseptic technique; tegument treatment (kept intact, with side cuts and elimination); lighting conditions (light or dark); and two cultivation media [ms medium, with salts concentration reduced by half (m1) and ms medium, full strenger (m2)] have been tested. in both cases, 30 g l-1 sucrose and 7 g l-1 agar were added, with ph adjusted to 5.6 with naoh. cultivation took place in a growth chamber. it is viable to obtain healthy artichoke plantlets in short time (seven days), to be used as a source of explants from in vitro seed germination without the tegument (77,5% of germination), using the m1 or m2 culture medium and growth chamber without light. in these conditions, the asepsis of seeds can be done with alcohol 70% during 30 minutes and the subsequent immersion in solution of 2% of active chlorine during 10 minutes, before the removal of the tegument.
Caracteriza??o citogenética, viabilidade de pólen e hibrida??o artificial em gérbera
Cardoso, Raquel DL;Grando, Magali F;MS-Basso, Simone;Segeren, Monique Inês;Augustin, Lizete;Suzin, Marilei;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000100008
Abstract: this work was conducted to confirm the chromosomes number of gerbera hybrida hort. cultivars, to determine the chromosomes number in the non commercial accessions of gerbera sp., and to estimate the pollen viability and the possibility of crossings among different accessions. root-tip and pollen were collected from six cultivars and seven non commercial accessions. the collected material was stained with acetic carmim at 45%. the chromosome counting was performed in metafasic intact cells and the pollen viability estimated by counting the number of viable and non viable pollen grains. the crossing possibility among cultivars and among cultivars and non commercial accessions was evaluated through the hybridization between the feminine cv. terra fame and a8 and masculine genitors, cvs. cariba and azteca. all accessions presented fifty chromosomes, indicating that the morphological variation in the capitulum (simple, semidouble and double) is not due to the chromosome number mutation or polyploidy. the evaluated accessions displayed high pollen viability, varying from 87.67% to 99.27. the seed formation was 4.46% in the crossings among cultivars, and 50% among cultivar and non commercial accession of gerbera. the genomic compatibility among the accessions, the high pollen viability and the success in the seed formation among commercial and non-commercial accessions, reveal the possibility of hybrid production with new allelic combination and transference of desirable traits from the non-commercial to the commercial accessions.
Gene Expression: A Review on Methods for the Study of Defense-Related Gene Differential Expression in Plants  [PDF]
Alice Casassola, Sandra Patussi Brammer, Márcia Soares Chaves, José Ant?nio Martinelli, Magali Ferrari Grando, Norimar D’ávila Denardin
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.412A3008

The plant genes involved in cellular signaling and metabolism have not been fully identified, while the function(s) of many of those which have are as yet incompletely characterized. Gene expression analysis allows the identification of genes and the study of their relationship with cellular processes. There are several options available for studying gene expression, including the use of cDNA and microarray libraries and techniques such as suppression subtractive hybridization (SSH), differential display (DD), RNA fingerprinting by arbitrary primed PCR (RAP), expressed sequence tags (EST), serial analysis of gene expression (SAGE), representational difference analysis (RDA), cDNA-amplified fragment length polymorphism (cDNA-AFLP) and RNA sequencing (RNA-Seq). Focusing on defense-related processes in plants, we present a brief review and examples of each of these methodologies and their advantages and limitations regarding the study of plant gene expression.

Two point deterministic model for acquisition of in vitro pollen grain androgenetic capacity based on wheat studies
Grando, Magali Ferrari;Moraes-Fernandes, Maria Irene B. de;
Brazilian Journal of Genetics , 1997, DOI: 10.1590/S0100-84551997000300018
Abstract: this article discusses, from the standpoint of cellular biology, the deterministic and indeterministic androgenesis theories. the role of the vacuole and of various types of stresses on deviation of the microspore from normal development and the point where androgenetic competence is acquired are examined. based on extensive literature review and data on wheat studies from our laboratory, a model for androgenetic capacity of pollen grain is proposed. a two point deterministic model for in vitro androgenesis is our proposal for acquisition of androgenetic potential of the pollen grain: the first switch point would be early meiosis and the second switch point the uninucleate pollen stage, because the elimination of cytoplasmatic sporophytic determinants takes place at those two strategic moments. any abnormality in this process allowing the maintenance of sporophytic informational molecules results in the absence of establishment of a gametophytic program, allowing the reactivation of the embryogenic process
Indu??o de embriogênese somática em diferentes explantes de aveia (Avena sativa L.)
Bispo, Noryam Bevian;Grando, Magali Ferrari;Augustin, Lizete;Suzin, Marilei;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000300047
Abstract: three experiments were carried out to evaluate the embryogenic callus formation potential of three types of explants in oat (avena sativa l.) (immature embryo, mature embryo and coleptile segments). each experiment was conduced with one explant, five cultivars (upf15, upf16, upf18, upfa20 and or3) and two induction callus medium (m1 = ms + 2 4-d and m2 = ms + 2 4-d + bap), being analyzed the frequency of embryogenic calli at 60 days of cultivation, callus fresh weight (mg) at 90 and 120 days and callus growth rate during 30 days period. the influence of genotype on the embryogenic callus frequency was detected only when using the immature embryo explant. the culture medium did not influence the frequency of callus in any of the three tested explants. the mature embryo was not suitable for the induction of oat somatic embryogenesis. the coleoptile and immature embryo explants present a similar embryogenic capacity, producing 24 and 29% of embryogenic calli, respectively. however, the coleoptile can be considered the ideal explant due to its genotypic independence, high embryogenic callus growth rate (328% in 30 days), as well as easy and quick availability.
Desempenho in vitro e agron?mico de cultivares micropropagadas de morangueiro em vários subcultivos
Calvete, Eunice Oliveira;Grando, Magali Ferrari;Gomide, Daniele Guerreiro;Maran, Ricardo Eoclides;Suzin, Marilei;Nienow, Alexandre Augusto;Cecchetti, Dileta;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000400005
Abstract: strawberry is a vegetative propagated plant, what makes it easier for virus contamination through infected plantlets. the use of matrix plants generated by in vitro shoot apex cultivation has being useful to regenerate free pathogens plants. to preserve the genetic identity and to maintain the high multiplication rate, most micropropagation protocols are based on four subcultures. with the objective to elevate the efficiency of the micropropagation process, two strawberry cultivars (oso grande and vila nova) were submitted to nine subcultures, and it was evaluated the multiplication rate and the occurrence of somaclonal variation. while cultivated in vitro, it was evaluated the multiplication rate, plant height and number of leaves by propagule. at the acclimatization stage it was determined the survival rate, leaf area, aerial and root fresh and dry weight. the clones obtained from the matrix plants were evaluated in relation to the agronomic behavior during the fruit production stage in a protected environment. the highest in vitro multiplication rate was observed during the second and third subculture, with the average of seven to eight propagules per explants, respectively. it was not observed phenotypic alterations in the two cultivars during the micropropagation process, acclimatization and growth in a protected environment. the oso grande cultivar presented higher agronomic performance when compared to the vila nova cultivar.
Retail Brand Equity: Measurements through Brand Policy and Store Formats  [PDF]
Magali Jara
American Journal of Industrial and Business Management (AJIBM) , 2018, DOI: 10.4236/ajibm.2018.83038
Abstract: This research extends findings on the retail brand equity in measuring the impact of its antecedents on the loyalty to the brand and to the store. This article raises questions about the sustainable created value by standard retail brands mostly oriented to functional components. The retail branding policy and store formats moderate results. This research adopts a PLS-Path modeling to test the retail brand equity model and its variations and then to provide a synthetic calculation of the retail brand equity. Results show that the standard retail brand equity leads to the loyalty to the brand and to the store. It varies according to: 1) the store brand policy (store’s own-named) appears to be a winning option maximizing the loyalty; 2) the “popular store” format—combining supermarket and department store—reinforces the sustainable relationship with customers because of the high level of service. By calculating scores, Carrefour brand maximizes the relationships within the model. This work focuses on French standard retail brands excluding other retail brands (such as generics or premium). Results also focus on one product category. The retailer’s positioning variable extends previous contributions leading to more consistent results. This research is also focused on the antecedents of retail brand equity too less studied: Benefits (received from their consumption) and the packaging of the branded product are thus integrated. Hence, perspectives for practitioners are suggested.
Development of a coulometric method for the determination of gaseous sulfur compounds in urban atmospheres
Silva, Magali;Dick, Luis F. P.;
Journal of the Brazilian Chemical Society , 2000, DOI: 10.1590/S0103-50532000000200010
Abstract: a new procedure for the determination of atmospheric sulfur compounds was developed and applied during three months in the central area of the city of porto alegre- rs, and one month near an oil refinery located close to this city. the method is based on the coulometric determination of the tarnishing rate of pure silver. the results were compared with the method of pararosaniline, which is being currently used in some of the studied sites for the determination of so2. the results obtained showed a good correlation between the coulometric method and the pararosaniline method, when only so2 is present, as usually is the case of urban atmospheres. when h2s is also present, as in the case of industrial areas, the methodology tends to strongly overestimate so2 concentration.
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