Introduction: H. pylori is a primary pathogen isolated by Warren and Marshall in 1983. They called it as Campylobacter Pylori and in 1989 Goodwin et al. renamed it as Helicobacter pylori. Helicobacter pylorus is one of the responsible factors for causing gastritis, Peptic ulcer disease and is strongly associated with gastric carcinoma and gastric Malt lymphoma. Materials and Methods: In this study 300 biopsies were collected at Deccan College of Medical Sciences and Allied Hospitals, Hyderabad, India. Of these, 101 patients had peptic ulcer, 95 patients had gastritis and 4 had gastric carcinoma. A total of four gastric biopsy specimens were collected. One was used for culturing H. pylori, one for histological lesions and the remaining two one each from the antrum & corpus was collected in phosphate buffered saline for the DNA analysis. Results: Helicobacter pylori were isolated from many of the biopsies and the identification of Helicobacter pylori was confirmed in 200 biopsies with colony characteristics, Biochemical tests, and 16S rRNA amplification. The presence of VacA marker was detected by using appropriate primers. From the data obtained in our study, in a total of 169/200 isolates s1 region was found in 84.1% and s2 was found in 36/200 i.e. 18% of the isolates. In the middle region, m1awas found in 60/200 which was 30%, m1b was found in 60/200 i.e. 60% and m2 was found in 114/200 i.e. 57.1% of the isolates. Conclusion: Thus, VacA detection might be helpful for determination of which patients are at highest risk for severe clinical outcomes such as duodenal ulcer, gastric ulcer or gastric carcinoma and eventually, to define strategies for the treatment or prevention of H. pylori infection.
Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed
food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in
1000 people across India. The Ratio of Male: female was 700: 300 with age group
ranging from 20 - 39. Three gastric biopsies were collected for identification
of Helicobacter pylori. PCR
(Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis
showed the prevalence of H. pylori as
70.8% in individuals frequently consuming food prepared under less hygienic condition
(street vendor’s food) whereas 60% (less prevalence) in individuals consuming
food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume
daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s
food is not directly involved in the transmission of this bacterium. The
unhygienic preparation of food and poor personal hygiene play a vital role in
the transmission of these bacteria.
In this article we present simple and feasible idea to introduce electrolysis experiments using accessible materials in the classroom. We use plastic syringes and pipettes to build different shapes of Hoffman apparatus for electrolysis. This activity was introduced to a group of 20 teachers from the Arab sector in Israel to examine their impressions about the activity and investigate the feasibility of implementing the activity in their classrooms.