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Search Results: 1 - 6 of 6 matches for " Madhavarao Thirtham "
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Study on the incidence of Salmonella enteritidis in Poultry and meat Samples by Cultural and PCR Methods
Putturu Ramya,Thirtham Madhavarao,Lakkineni Venkateswara Rao
Veterinary World , 2012, DOI: 10.5455/vetworld.2012.541-545
Abstract: Aim: To study the incidence of S.enteritidis in poultry and meat samples by cultural and PCR methods. Materials and Methods: A total of 130 samples (25 each of chicken, mutton, poultry faeces, cloacal samples and 10 each of liver, spleen and kidney) collected from different sources were subjected to cultural and PCR methods for the presence of Salmonella and Salmonella enteritidis. Primers for invA and sefA gene were used for Salmonella and S.enteritidis respectively. Results: Out of 130 samples, 87 were positive for Salmonella spp. i.e. chicken-16(64%), mutton-12(48%), faeces-23(92%), cloacal swabs-23(92%), liver-5(50%), spleen and kidney samples-4(40%) each by PCR methods, whereas 77 were positive by cultural method i.e. chicken-14(56%), mutton-10(40%), faeces-22(88%), cloacal swabs-21(84%), liver-4(40%), spleen and kidney-3(30% each). Out of 87 positive for Salmonella by PCR method, 59(chicken-12, mutton-7, faeces-17, cloacal swabs-15, liver-3, spleen-2, kidney-3) were positive for S.enteritidis. High incidence of S.enteritidis (68%) in all the above samples are indicative of unhygienic conditions in poultry farms. Selective enrichment with Rappaport-Vassilidias (RV) broths and Tetrathionate (TT) broths were superior over Selenite-F (SF) and Selenite cysteine (SC) broths. Conclusions: High incidence of S.enteritidis was seen in most of poultry samples like chicken, kidney, liver and it's faeces than mutton, which was indicative of contamination of S.enteritidis is more prevalent in poultry farms. [Vet World 2012; 5(9.000): 541-545]
Antimicrobial sensitivity and resistance of Salmonella Enteritidis isolated from natural samples
Ramya Putturu,Madhavarao Thirtham,Tirupati Reddy Eevuri
Veterinary World , 2013, DOI: 10.5455/vetworld.2013.185-188
Abstract: Aim: To test the sensitivity of S. Enteritidis for selected antibiotics. Materials and Methods: S. Enteritidis isolates obtained from different samples of chicken, mutton, turkey meat, faecal and cloacal samples of poultry and turkey, eggs, water and feed were subjected for sensitivity and resistance to selected antibiotics like- Chloramphenicol (30μg), Gentamicin (10 μg), Nalidixic Acid (30 μg), Tetracycline (30 μg), Ciprofloxacin (5 μg), Amikacin (30 μg), Amoxicillin (25 μg), Ampicillin (10 μg), Streptomycin (10 μg) and Sulfonamide (30 μg). Antimicrobial susceptibility of the isolates was established by the disk diffusion assay with MH (Muller-Hinton) agar in accordance with French National Antibiogram Committee Guidelines. Results: The sensitivity of S. Enteritidis was 100% for ciprofloxacin followed by chloramphenicol and amikacin (96%), gentamycin (90%), amoxicillin (82%), streptomycin (80%), tetracycline (76%), nalidixic acid (68%), ampicillin (58%) and sulfonamide (10%). The resistance was highest for sulfonamide (76%) followed by ampicillin (32%), nalidixic acid (30%) and 6-20% for gentamycin, amoxicillin and tetracycline. Conclusion: S. Enteritidis isolates were more sensitive to ciprofloxacin, chloramphenicol, amikacin, gentamycin, streptomycin,amoxicillin and tetracyclines and less sensitive to sulfonamides. Higher resistance was observed with sulfonamide followed by ampicillin and nalidixic acid. [Vet World 2013; 6(4.000): 185-188]
Combined central membranous and anterior suture cataract in a family
Sundaresan K,Madhavarao G
Indian Journal of Ophthalmology , 1968,
Micro-Modelling Approach to Predict the Influence of Hydrogen Pressure on Short Crack Behaviour  [PDF]
Claude Lincourt, Jacques Lanteigne, Madhavarao Krishnadev, Carl Blais
Modeling and Numerical Simulation of Material Science (MNSMS) , 2013, DOI: 10.4236/mnsms.2013.33A006
Abstract: A micromechanical model, based on the FEA (finite element analysis), was developed to estimate the influence of hydrogen pressure on short crack behaviour. Morphology of voids has important connotations in the development of the model. Stress intensity factor was calculated for different crack geometries under hydrogen pressure. The analysis indicates that the form factor of a crack emerging from a round void will be less affected by trapped hydrogen pressure-compared to an elongated void. This analysis reinforces the beneficial effect of inclusion shape control in reducing significantly the detrimental effect of hydrogen.
Calculation of the Stress Intensity Factor in an Inclusion-Containing Matrix  [PDF]
Claude Lincourt, Jacques Lanteigne, Madhavarao Krishnadev, Carl Blais
Modeling and Numerical Simulation of Material Science (MNSMS) , 2019, DOI: 10.4236/mnsms.2019.92002
Abstract: The intent of this paper is to propose an engineering approach to estimate the stress intensity factor of a micro crack emerging from an inclusion in relation with the morphology of the inclusion and its relative stiffness with the matrix. A micromechanical model, based on the FEA (finite element analysis) of the behavior of cracks initiated at micro structural features such as inclusions, has been developed using LEFM (Linear Elastic Fracture Mechanics) to predict the stress intensity factor of a micro crack emerging from an inclusion. Morphology of inclusions has important connotations in the development of the analysis. Stress intensity factor has been estimated from the FEA model for different crack geometries. Metallographic analysis of inclusions has been carried out to evaluate the typical inclusion geometry. It also suggests that micro cracks less than 1μm behave differently than larger cracks.
High definition spectral domain optical coherence tomography of retinal pigment epithelial rip in a case of sympathetic ophthalmia
Padmamalini Mahendradas, Kavitha Avadhani, Bhanumathi Madhavarao, Anand Vinekar, Kodandaramaiah Sahana, Rohit Shetty, Bhujang K Shetty
Journal of Ophthalmic Inflammation and Infection , 2013, DOI: 10.1186/1869-5760-3-19
Abstract: Findings: A 34-year-old female was diagnosed to have sympathetic ophthalmia on treatment with systemic steroids. Vision did not improve in spite of aggressive systemic steroid therapy. On examination, patient had large retinal pigment epithelial rip nasal to the disc with exudative retinal detachment which was documented with FFA, ICG, and OCT. RPE rip is responsible for the persistent exudative retinal detachment in the right eye.RPE rip can cause decreased vision due to persistence of retinal detachment in a case of sympathetic ophthalmia.
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