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Search Results: 1 - 10 of 485913 matches for " Ma a Vodovnik "
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Using a different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles
Ma a Zorec, Ma a Vodovnik, Romana Marin ek Logar
Acta agriculturae Slovenica , 2011, DOI: 10.2478/v10014-011-0009-5
Abstract: A total of 11 ruminal strains currently assigned to Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans were cultivated in two different media, rumen fluid containing M2 and short-chain fatty acid (SCFA) containing M330, and their cellular fatty acid methyl esters (FAME) and dimethylacetals (DMA) were analyzed using gas chromatography. A comparison of the FAME/DMA compositions revealed that the difference in SCFA contents in the growth medium induced a pronounced quantitative effect on the cellular branched-chain fatty acid and aldehydes proportions only in the P. xylanivorans strains. This study shows that FAME/DMA analysis is a powerful chemotaxonomic tool in the group of phenotypically similar rumen butyrivibria especially when the influence of the growth medium is evaluated.
Methylmercury inhibits growth and induces membrane changes in Pseudomonas putida
Ma a Vodovnik, Mirjana Bistan, Ma a Zorec, Romana Marin ek Logar
Acta agriculturae Slovenica , 2010, DOI: 10.2478/v10014-010-0020-2
Abstract: A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess toxicity of the environmentally relevant concentrations of mercury species (MeHg and Hg(II)) that represent important pollutants of aquatic ecosystems at sites of industrial or mining activities. In addition to direct monitoring of bacterial growth, we also analyzed fatty acid profiles of exposed and non-exposed cultures to determine possible toxic effects manifested on membrane level. The results showed that exposure of P. putida to Hg(II) in concentrations of 0.2-200 μg/L did not have any significant effects on growth nor fatty acid composition of exposed bacterial culture. On the other hand, when bacteria were exposed to up to 1600-times lower concentrations of MeHg (0.12-12.5 μg/L), growth inhibition as well as significant changes in fatty acid composition were detected. Observed adaptive membrane changes due to MeHg exposure were similar to those associated with responses to organic solvents and some other membrane-disrupting compounds.
Cellulosomics, a Gene-Centric Approach to Investigating the Intraspecific Diversity and Adaptation of Ruminococcus flavefaciens within the Rumen
Jennifer M. Brulc, Carl J. Yeoman, Melissa K. Wilson, Margret E. Berg Miller, Patricio Jeraldo, Sadanari Jindou, Nigel Goldenfeld, Harry J. Flint, Raphael Lamed, Ilya Borovok, Maa Vodovnik, Karen E. Nelson, Edward A. Bayer, Bryan A. White
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0025329
Abstract: Background The bovine rumen maintains a diverse microbial community that serves to break down indigestible plant substrates. However, those bacteria specifically adapted to degrade cellulose, the major structural component of plant biomass, represent a fraction of the rumen microbiome. Previously, we proposed scaC as a candidate for phylotyping Ruminococcus flavefaciens, one of three major cellulolytic bacterial species isolated from the rumen. In the present report we examine the dynamics and diversity of scaC-types both within and between cattle temporally, following a dietary switch from corn-silage to grass-legume hay. These results were placed in the context of the overall bacterial population dynamics measured using the 16S rRNA. Principal Findings As many as 117 scaC-types were estimated, although just nineteen were detected in each of three rumens tested, and these collectively accounted for the majority of all types present. Variation in scaC populations was observed between cattle, between planktonic and fiber-associated fractions and temporally over the six-week survey, and appeared related to scaC phylogeny. However, by the sixth week no significant separation of scaC populations was seen between animals, suggesting enrichment of a constrained set of scaC-types. Comparing the amino-acid translation of each scaC-type revealed sequence variation within part of the predicted dockerin module but strong conservation in the N-terminus, where the cohesin module is located. Conclusions The R. flavefaciens species comprises a multiplicity of scaC-types in-vivo. Enrichment of particular scaC-types temporally, following a dietary switch, and between fractions along with the phylogenetic congruence suggests that functional differences exist between types. Observed differences in dockerin modules suggest at least part of the functional heterogeneity may be conferred by scaC. The polymorphic nature of scaC enables the relative distribution of R. flavefaciens strains to be examined and represents a gene-centric approach to investigating the intraspecific adaptation of an important specialist population.
Expression of Cellulosome Components and Type IV Pili within the Extracellular Proteome of Ruminococcus flavefaciens 007
Maa Vodovnik, Sylvia H. Duncan, Martin D. Reid, Louise Cantlay, Keith Turner, Julian Parkhill, Raphael Lamed, Carl J. Yeoman, Margret E. Berg. Miller, Bryan A. White, Edward A. Bayer, Romana Marin?ek-Logar, Harry J. Flint
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065333
Abstract: Background Ruminococcus flavefaciens is an important fibre-degrading bacterium found in the mammalian gut. Cellulolytic strains from the bovine rumen have been shown to produce complex cellulosome structures that are associated with the cell surface. R. flavefaciens 007 is a highly cellulolytic strain whose ability to degrade dewaxed cotton, but not Avicel cellulose, was lost following initial isolation in the variant 007S. The ability was recovered after serial subculture to give the cotton-degrading strain 007C. This has allowed us to investigate the factors required for degradation of this particularly recalcitrant form of cellulose. Methodology/Principal Findings The major proteins associated with the bacterial cell surface and with the culture supernatant were analyzed for R. flavefaciens 007S and 007C grown with cellobiose, xylan or Avicel cellulose as energy sources. Identification of the proteins was enabled by a draft genome sequence obtained for 007C. Among supernatant proteins a cellulosomal GH48 hydrolase, a rubrerthyrin-like protein and a protein with type IV pili N-terminal domain were the most strongly up-regulated in 007C cultures grown on Avicel compared with cellobiose. Strain 007S also showed substrate-related changes, but supernatant expression of the Pil protein and rubrerythrin in particular were markedly lower in 007S than in 007C during growth on Avicel. Conclusions/Significance This study provides new information on the extracellular proteome of R. flavefaciens and its regulation in response to different growth substrates. Furthermore it suggests that the cotton cellulose non-degrading strain (007S) has altered regulation of multiple proteins that may be required for breakdown of cotton cellulose. One of these, the type IV pilus was previously shown to play a role in adhesion to cellulose in R. albus, and a related pilin protein was identified here for the first time as a major extracellular protein in R. flavefaciens.
Management in pravo = Management and the Law
Zvone Vodovnik
Management , 2006,
Abstract: The article analyses the legal concept of management. The conclusion is that it is not a legal category. Management is the object of research of various scientific disciplines, each of them from a different perspective. It is a notion that determines the processes, subjects taking decision within the organization. In this regard, there are also connected rights, duties and liabilites of individuals and groups having influence within this process. Special focus is pleaced on the relationship between managers and employers and on the nature of the managers’ powers and activities as regards the termination and decision about rights, duties and liabilities of employees.
Breast metastasis from a renal cell cancer
Ahmed Alzaraa, Aleksandar Vodovnik, Hugh Montgomery, Mohammed Saeed, Narinder Sharma
World Journal of Surgical Oncology , 2007, DOI: 10.1186/1477-7819-5-25
Abstract: An 81-year-old lady was seen in the breast clinic for a right breast mass after sustaining a fall. Clinical examination and investigations revealed a metastatic cancer from a renal primary. She received surgical treatment only and is under regular follow-up in the oncology clinic.The treatment strategy for metastatic breast diseases is based on a proper assessment of such cases by surgeons, radiologists and histopathologists.Metastases to the breast from extramammary tumours are uncommon, and metastatic renal cell carcinoma to the breast is extremely rare [1]. We report a metastasis to the breast from a renal primary with the radiological and histopathological features.An 81 years old lady had a right radical nephrectomy in 1999 for conventional renal cell cancer (RCC). She was discharged from the urology and oncology clinics in 2004 after 5 1/2 years follow-up with no signs of local or regional recurrence.In December 2004, she noticed a lump in the right breast after sustaining a fall. She was referred to the breast clinic in July 2005 for further assessment.Clinically, she had a mass in the upper outer quadrant of the right breast. The left breast was normal and there was no axillary lymphadenopathy. Abdominal examination was normal.Radiology confirmed a 17 × 13 × 9 mm well circumscribed hypoechoic mass in the right upper quadrant of the right breast (Figure 1). The mass was core biopsied.The histopathological examination revealed tumour growth consistent with conventional renal cell carcinoma. Tumour cells strongly expressed vimentin. CT scan of the chest and abdomen showed a 12 mm mass in the right breast and a 2.7 cm metastatic deposit at the right renal bed (Figures 2 and 3). The lungs and the liver were normal. The lump was excised in October 2005.The gross examination of the specimen confirmed metastasis from a renal primary (Figure 4). There was no evidence of in situ ductal or lobular disease.The patient was offered Interferon treatment, but she preferred
The Control System Modeling Language
Klemen Zagar,Mark Plesko,Matej Sekoranja,Gasper Tkacik,Anze Vodovnik
Physics , 2001,
Abstract: The well-known Unified Modeling Language (UML) describes software entities, such as interfaces, classes, operations and attributes, as well as relationships among them, e.g. inheritance, containment and dependency. The power of UML lies in Computer Aided Software Engineering (CASE) tools such as Rational Rose, which are also capable of generating software structures from visual object definitions and relations. UML also allows add-ons that define specific structures and patterns in order to steer and automate the design process. We have developed an add-on called Control System Modeling Language (CSML). It introduces entities and relationships that we know from control systems, such as "property" representing a single controllable point/channel, or an "event" specifying that a device is capable of notifying its clients through events. Entities can also possess CSML-specific characteristics, such as physical units and valid ranges for input parameters. CSML is independent of any specific language or technology and generic such that any control system can be described with it. Simple transformation scripts map CSML defined structures to APIs and tools such as EPICS, CDEV, SCADA, Abeans, BACI and generate the appropriate database or source files. Advantages of control system development with CSML are discussed on a concrete example of a bending magnet's power supply in a synchrotron accelerator.
Antithrombin significantly influences platelet adhesion onto immobilized fibrinogen in an in-vitro system simulating low flow
Robert Loncar, Uwe Kalina, Volker Stoldt, Volker Thomas, Rüdiger E Scharf, Aleksandar Vodovnik
Thrombosis Journal , 2006, DOI: 10.1186/1477-9560-4-19
Abstract: Platelets in anticoagulated whole blood (29 healthy blood donors) were labelled with fluorescence dye and perfused through a rectangular flow chamber (shear rates of 13 s-1 to 1500 s-1). Platelet adhesion onto fibrinogen-coated slips was assessed using a fluorescence laser-scan microscope and compared to the plasma antithrombin activity. Additionally the effect of supraphysiological AT supplementation on platelets adhesion rate was evaluated.Within a first minute of perfusion, an inverse correlation between platelet adhesion and plasma antithrombin were observed at 13 s-1 and 50 s-1 (r = -0.48 and r = -0.7, p < 0.05, respectively). Significant differences in platelet adhesion related to low (92 ± 3.3%) and high (117 ± 4.1%) antithrombin activity (1786 ± 516 U vs. 823 ± 331 U, p < 0.05) at low flow rate (13 s-1, within first minute) have been found. An in-vitro supplementation of whole blood with antithrombin increased the antithrombin activity up to 280% and platelet adhesion rate reached about 65% related to the adhesion rate in a non-supplemented blood (1.25 ± 0.17 vs. 1.95 ± 0.4 p = 0.008, respectively).It appears that antithrombin in a low flow system suppresses platelet adhesion onto immobilized fibrinogen independently from its antithrombin activity. A supraphysiological substitution of blood with antithrombin significantly reduces platelet adhesion rate. This inhibitory effect might be of clinical relevance.The scope of the problem of arterial and venous thrombosis is staggering since at least 5 million adults in the United States alone suffer from related symptoms. About 50% of the annual non-accidental deaths in the United States are caused by thrombi predominantly composed of platelets in the coronary or cerebral arteries.Antithrombin (AT), in the past also referred as antithrombin III, is a potent inhibitor of the coagulation cascade [1]. Although the name, antithrombin, implies that it works only on thrombin, it actually serves to inhibit virtually all o
Platelet adhesion onto immobilized fibrinogen under arterial and venous in-vitro flow conditions does not significantly differ between men and women
Robert Loncar, Reiner B Zotz, Christoph Sucker, Aleksandar Vodovnik, Mario Mihalj, Rüdiger E Scharf
Thrombosis Journal , 2007, DOI: 10.1186/1477-9560-5-5
Abstract: Platelets in whole anticoagulated blood were labelled with the fluorescence dye Mepacrine and perfused through the rectangular flow chamber over glass cover slips coated with fibrinogen (shear rates of 50 s-1, 500 s-1 and 1500 s-1). A fluorescence laser-scan microscope was used for visualisation and quantification of platelet adhesion at 15 seconds, 1 and 5 minutes after the start of perfusion.During perfusion, the platelet adhesion linearly increased in regard to exposition time and shear rate. After five minutes of perfusion the platelet adhesion onto immobilized fibrinogen showed no significant gender related difference, neither at 50 s-1 nor at 500 s-1 and 1500 s-1 (p > 0.05), respectively. No significant difference in platelet adhesion onto immobilized fibrinogen, in regard to the menopausal status, was either observed (p > 0.05).In our in vitro experimental system, hormonal differences between men and women did not influence platelet adhesion onto immobilized fibrinogen, neither under venous nor under arterial rheological conditions.Ischemic heart and cerebrovascular disease are leading causes of morbidity and mortality in the western world and are steadily increasing incidence in the third world as well [1,2]. Epidemiological studies [3] indicate that these diseases result from complex interactions between genetic susceptibility factors, chronic environmental influences (e.g. hormonal imbalance, smoking, obesity) and established, intercurrent disorders (e.g. diabetes, hypertension, dyslipidemia, or hyperhomocysteinemia). The most devastating complication of these disorders is acute myocardial infarction or stroke resulting from the formation of occlusive thrombus at the site of ruptured atherosclerotic plaque [1-4]. Platelet-dependent thromboembolism is an underlying mechanism of arterial thrombosis and the critical role of platelets in this process is now widely accepted [1,5]. Its participation in the arterial thrombosis is centered on their adhesive proper
Suprascapular Nerve Entrapment Secondary to Compression at Suprascapular Notch: A Case Report
A Fazal, MA Wajid
East African Orthopaedic Journal , 2012,
Abstract: Suprascapular nerve entrapment at spinoglenoid notch is rare but well documented. Kopell and Thompson (1) are always credited with providing the first description of the suprascapular nerve entrapment syndrome in English literature (2,3) but in fact the first description of suprascapular nerve entrapment was provided by André Thomas in La Presse Médicale, entitled “La paralysie du muscle sous-épineux in 1936” (4,5). We report a case of suprascapular nerve compression at suprascapular foramen.
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