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Search Results: 1 - 10 of 1618 matches for " MOHAMAD SADIKIN "
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Properties of Folate Binding Protein Purified from Cow’s Milk
SUBANDRATE,DWIRINI RETNO GUNARTI,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2012,
Abstract: Folic acid played an important role in the metabolism of the body. To measure the serum folic acid levels could use the folate binding protein (FBP) from cow’s milk with a technique analogous to ELISA. The aims of this study were to identify characteristics of FBP from cow’s milk and binding capacity of FBP to folic acid and to purify FBP from other whey protein passed through DEAE-cellulose chromatography column. Each of DEAE-cellulose peaks was passed in affinity chromatography column. FBP was released from affinity column with sodium acetate buffer pH 3.5. The purity of obtained FBP was demonstrated by a single spot in SDS-PAGE analysis and the estimated molecular weight of FBP was around 31 kDa. Our study indicated that 1 mol FBP bound 1 mol folic acid. Alkylation with iodoacetic acid decreased the binding capacity of FBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of folate binding capacity of FBP after -mercaptoethanol treatment. In contrary, the folate binding didn need Ca2+ ion, as indicated by EDTA test which gave the same result as control.
Isolation and Purification of Thiamine Binding Protein from Mung Bean
DWIRINI RETNO GUNARTI,HANIFAH RAHMI,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2013,
Abstract: Thiamine has fundamental role in energy metabolism. The organs mostly sensitive to the lack of thiamine levels in the body are the nervous system and the heart. Thiamine deficiency causes symptoms of polyneuritis and cardiovascular diseases. Because of its importance in the metabolism of carbohydrates, we need to measure the levels of thiamine in the body fluids by using an easy and inexpensive way without compromising the sensitivity and selectivity. An option to it is thiamine measurement based on the principle of which is analogous to ELISA, in which a thiamine binding protein (TBP) act by replacing antibodies. The presence of TBP in several seeds have been reported by previous researchers, but the presence of TBP in mung beans has not been studied. This study was aimed to isolate and purify TBP from mung bean. The protein was isolated from mung bean through salting out by ammonium sulphate of 40, 70, and 90% (w/v). TBP has a negative charge as shown by cellulose acetate electrophoresis. The result obtained after salting out by ammonium sulphate was further purified bymeans of DEAE-cellulose chromatography and affinity chromatography. In precipitation of 90% of salting out method, one peak protein was obtained by using affinity chromatography. The protein was analyzed by SDS PAGE electrophoresis. The result of SDS PAGE electrophoresis showed that TBP has a molecular weight of 72.63 kDa.
Isolation and Characterization of Chelonia mydas Myoglobin
MOHAMAD SADIKIN,RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO
HAYATI Journal of Biosciences , 2012,
Abstract: Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied icroscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by ephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins.
Blood Gasses Contents of Green Turtle (Chelonia mydas) Hatch Treated by Different Temperatures
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2011,
Abstract: The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used to support for turtle hatchery in Indonesia.
Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2010,
Abstract: An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.
Expression of Simian Retrovirus Type D Serotype 2 Envelope in Insect Cell Using Baculovirus Expression Vector System
UUS SAEPULOH,DIAH ISKANDRIATI,MOHAMAD SADIKIN,JOKO PAMUNGKAS
Microbiology Indonesia , 2009,
Abstract: Simian retrovirus type-D (SRV) is a causative agent of simian acquired immunodeficiency syndrome in Asian macaques, and can serve as a viral model in understanding of retrovirus infection because of some similarities to human AIDS pathogenesis. Study of infection and pathogenesis of SRV in macaques could be a strategy of vaccine and antiviral development for preventive and therapeutic purposes. We expressed the SRV-2 envelope gene using baculovirus expression vector system and transfected it to Spodoptera frugiferda insect cell line for SRV-2 recombinant protein production. Analysis using PCR and sequencing technique of recombinant in the passage-3 viral stock indicated the occurrence of recombination between SRV-2 envelope and baculovirus genome. Purification using immobilized metal ion affinity chromatography Ni2+-NTA to recombinant protein could minimize the presence non-specific proteins. The SDS-PAGE analysis showed a specific protein for SRV-2 gp70 envelope. Western blot analysis of this purified protein indicated a specific reaction with anti-SRV-2 antibody positive of Macaca fascicularis serum shown as SRV-2 gp70 envelope band.
Ischemic Monomelic Neuropathy after a Brachio-Cephalic AVF Creation: A Case Report and Review of Literature  [PDF]
Mohamad Taha
World Journal of Cardiovascular Surgery (WJCS) , 2017, DOI: 10.4236/wjcs.2017.73004
Abstract: Ischemic monomelic neuropathy (IMN) is an under-diagnosed and disabling complication of upper extremity hemodialysis access. The hemodynamic disturbance that occurs during the time of access creation can lead to both neurologic and ischemic complications. These complications are most commonly seen following proximal procedures involving the upper limbs. Diagnosis and treatment are often delayed, and even with early intervention, neurologic dysfunction may be irreversible. IMN can lead to significant long-term disability. Almost all cases of IMN occur with brachial artery-based access, procedures and the vast majority of patients are diabetic and females. I report a case of IMN after left BC AVF operation which was treated successfully by early access ligation, providing a review of the literature.
PERANCANGAN MANAJEMEN KINERJA PENYELENGGARAAN PENDIDIKAN DAN PELATIHAN DENGAN ANCANGAN MANAGEMENT BY OBJECTIVES (MBO) DAN PERSPEKTIF BALANCED SCORECARD
Ika Susanti,Aida Vitaya Hubeis,Sadikin Kuswanto
Jurnal Manajemen & Agribisnis , 2012,
Abstract: This research is aimed to make a performance management design based on MBO and Balanced Scorecard perspective approach in order to improve education and training administration. Performance accountability stimulates public sector organizations to improve their performance continuously and to build a performance management system that is oriented not only toward the budget and its realization, but also toward results. This paper discusses MBO and Balanced Scorecard perspective approach in planning and controlling its programs. In addition, this model can be used as a strategic controller for the management to measure the organization’s success and failure. This is a qualitative research approached by a case study using the method of participant observation through questionnaires, in depth-interviews, and focused group discussions. The success of education and training administration is determined by performance achievements of the individual, division, and organization that include financial aspect, participants of the education and training program, Standard Operating Procedure (SOP), administrators of the program, technology, and innovation. The resulted design is a four-stage flowchart describing planning, implementation, measurement, and performance evaluation. Keywords : Performance Management, Design, Education and Training, Management by Objectives (MBO), Balanced Scorecard Perspective
AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI KLABET (Trigonellafoenum-graecum L.): PENGUKURAN KADAR GLUTATION TIKUS DIABETES
Lucie Widowati,Moh. Sadikin,B. Wahjoedi
Media of Health Research and Development , 2012,
Abstract: Uji aktivitas antioksidan dari ekstrak biji klabet (frigonella foenum-graecum L.) dilakukan pada tikus putih hiperglikemia. Keadaan hiperglikemia adalah akibat induksi aloksan tetrahidrat 125 mg/kg bb. Pada keadaan hiperglikemia terjadi penurunan kadar antioksidan glutation (GSH) hampir 50%. Kadar GSH plasma diukur dengan cara Ellman. Pemberian bahan uji ekstrak klabet dosis 140; 240 dan 560 mg/200g bb. menyebabkan kenaikan kadar GSH plasma setelah 3 hari pemberian bahan uji sebesar 35,77; 66,83; 31,26% dari keadaan awal hiperglikemi dan kenaikan kadar GSH plasma setelah 7 hari pemberian bahan uji, sebesar 63,21; 90,67; 44,52% dari keadaan awal hiperglikemi. Aktivitas antioksidan terlihat pada kelompok dosis 140 mg/200g bb. and 280 mg/200g bb (p>0.05). Kata kunci: Trigonellafoenum-graecum L.; klabet; ekstrak etanol; antioksidan.
Incidence and Fetal Outcomes of HIV/Aids Patients at Hasan Sadikin General Hospital  [PDF]
Anita Rachmawati, Johannes C. Mose, Hasan Sadikin
Open Access Library Journal (OALib Journal) , 2017, DOI: 10.4236/oalib.1103704
Abstract:
Introduction: The increasing number of HIV/AIDS incidence over time begins to become global problems. One of the current concerns is the morbidity and mortality rates in the fetal outcomes patients with HIV/AIDS who can provide many complications. The importance of adequate pre-and antenatal care may help to reduce morbidity and mortality due to this condition. The purpose of this study is to determine incidence and morbidity of mother with HIV/AIDS and fetal outcomes after delivery in Hasan Sadikin General Hospital from 2011 to 2015. Method: With descriptive study, we use secondary data from medical record on Obstetrics and Gynecology Department. Results of fetal outcomes obtained from medical record on Perinatology Department and the management of HIV/AIDS in married couples and fetal after delivery was obtained from medical record on Teratai Clinic, Hasan Sadikin General Hospital. Results: The incidence of mother with HIV/AIDS who gave birth at Hasan Sadikin General Hospital from 2011 to 2015 tends to increase every year with the number of percentage is 0.06%, 0.29%, 0.24%, 0.72%, and 0.38% from total deliveries, respectively. Fetal outcomes vary from preterm labour 19.57%, low birth weight baby 15.22%, asphyxia 13.04%, small for gestational age (SGA) 4.35%, and early fetal death 2.17%. Conclusion: Incidence of delivery with HIV/AIDS tends to increase every year. Preterm labor has the highest morbidity on fetal outcomes.
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