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Search Results: 1 - 10 of 67057 matches for " MA Yong-Qing "
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A Brief Introduction to the Researches on Allelochemicals in Wheat Straw

HAN Qing-hua,MA Yong-qing,

中国生态农业学报 , 1994,
Abstract: Allelopathy is a natural exsiting phenomenon.In this paper,taking wheat straw as the studing object,a detailed information on its allelopathic phenomenon,extracting,indentification,isolation and bioassay testing as well as the aspects which influences thewheat straw allelochemicals releasing were listed.In addition,a brief introduction to the researches on allelochemicals in wheat straw and its utilization in the world are presented.
A Study on the Variation of Allelopathic Effect of Wheat Straw Mulching on Different Corn Varieties

MA Yong-qing,HAN Qing-hua,

中国生态农业学报 , 1993,
Abstract: In this paper pot experiment was conducted on the allelopathic effect of wheat straw water extract and wheat straw mulching on corn seed germination and early growth and development among different corn varieties.The results show that there is significat variation among different corn varieties in enduring allelopathic effect of wheat straw mulching.When the most endurable corn varieties are selected for growing under wheat straw mulching condition,the negative allelopathic effect on corn growth and development could be avoided.
Deletion or insertion in the first immunoglobulin-plexin-transcription (IPT) domain differentially regulates expression and tumorigenic activities of RON receptor Tyrosine Kinase
Qi Ma, Kun Zhang, Sunny Guin, Yong-Qing Zhou, Ming-Hai Wang
Molecular Cancer , 2010, DOI: 10.1186/1476-4598-9-307
Abstract: Two RON variants, RON160 and RONE5/6in with deletion and insertion in the first IPT domain, respectively, were molecularly cloned. RON160 was a splicing variant generated by deletion of 109 amino acids encoded by exons 5 and 6. In contrast, RONE5/6in was derived from a transcript with an insertion of 20 amino acids between exons 5 and 6. Both RON160 and RONE5/6in were proteolytically matured into two-chain receptor and expressed on the cell surface. RON160 was constitutively active with tyrosine phosphorylation. However, activation of RONE5/6in required ligand stimulation. Deletion resulted in the resistance of RON160 to proteolytic digestion by cell associated trypsin-like enzymes. RON160 also resisted anti-RON antibody-induced receptor internalization. These features contributed to sustained intracellular signaling cascades. On the other hand, RONE5/6in was highly susceptible to protease digestion, which led to formation of a truncated variant known as RONp110. RONE5/6in also underwent rapid internalization upon anti-RON antibody treatment, which led to signaling attenuation. Although ligand-induced activation of RONE5/6in partially caused epithelial to mesenchymal transition (EMT), it was RON160 that showed cell-transforming activities in cell focus formation and anchorage-independent growth. RON160-mediated EMT is also associated with increased motile/invasive activity.Alterations in the first IPT domain in extracellular region differentially regulate RON mediated tumorigenic activities. Deletion of the first IPT results in formation of oncogenic variant RON160. Enhanced degradation and internalization with attenuated signaling cascades could be the mechanisms underlying non-tumorigenic features of RONE5/6in.The RON (recepteur d'origine nantais) receptor tyrosine kinase belongs to the MET proto-oncogene family [1,2], which plays a critical role in epithelial cell homeostasis and tumorigenic development [3]. Expression of RON has been found mainly in cells of epi
Response of transgenic potato seedlings to allelopathic pressure and the effect of nutrients in the culture medium

Sheng-Peng Zuo,Xiu-Wei Li,Yong-Qing Ma,

生态学报 , 2010,
Effects of Wenshen Jianpi Recipe on chronic wound healing in rats
CAO Yong-Qing
Zhong Xi Yi Jie He Xue Bao , 2005,
Abstract: Objective: To study the effects of Wenshen Jianpi Recipe (WSJPR, a traditional Chinese medicine for warming kidney and invigorating spleen) on chronic wound healing and the mechanism. Methods: Ninety-six SD rats were randomly divided into 4 groups, with 24 rats in each group, and back wound was made in the rats. For rats in 3 of the 4 groups, hydrocortisone injection was administered to induce chronic wound. Rats in 2 of the 3 groups were treated with WSJPR and Xinpukang Granules (XPKG) respectively, and the rats in the other group were untreated. The rats in the fourth group were taken as control. The wound healing time and the width of new epidermis were observed, and the histomorphological changes and cell cycle of the granulation tissue, and the protein expressions of epidermal growth factor (EGF), transforming growth factor-β1 (TGF-β1) and fibronectin (FN) in the granulation tissue were tested with immunohistochemical technique and flow cytometry. Results: The wound healing time of the WSJPR-treated and XPKG-treated groups was (17.0±1.9) and (18.8±1.9) d respectively, much shorter than that of the untreated and control groups (P<0.05). On the 14th experiment day, the width of new epidermis of the WSJPR-treated and XPKG-treated groups was (3.73±0.19) and (3.21±0.15) mm respectively, much wider than that of the untreated and control groups (P<0.05). The numbers of angiogenesis, fibroblasts and cells in the S phase in WSJPR-treated and XPKG-treated groups were much higher than those in the untreated and control groups (P<0.05). Compared with the untreated and control groups, the protein expressions of EGF, TGF-β1 and FN in WSJPR-treated and XPKG-treated groups were higher (P<0.05). Conclusion: WSJPR can enhance the wound healing. It was likely through accelerating the cell proliferation and up-regulating the expressions of EGF, TGF-β1 and FN.
A Method of Hepatocyte Extraction Conjugated with HPLC is Established for Screening Potential Active Components in Chinese Medicines—Probing Herba Artemisiae Scopariae as an Exemplifying Approach
Min Hong,Hong-Yu Ma,Xiang-Rui Wu,Yong-Qing Hua,Quan Zhu,Hong-Wei Fan
Molecules , 2012, DOI: 10.3390/molecules17021468
Abstract: In order to establish an effective and quick method for screening potential bioactive compounds in Traditional Chinese Medicines (TCMs), hepatocytes were employed for extracting either bifendate, a clinical medicine for liver diseases, or chemicals in Herba Artemisiae Scopariae (A. Scopariae), a commonly used traditional Chinese medicine for remedying liver diseases such as hepatitis induced by viruses, chemicals or alcohol. After hepatocyte extraction the compounds were analyzed by HPLC, therefore this method was referrred to as hepatocyte extraction conjugated with HPLC (HE-HPLC). In the first part of this study, HE-HPLC showed that bifendate was extracted by hepatocytes and detected by HPLC-DAD which indicated the feasibility of this method. Then in the second part of the study, the potential active components in the A. scopariae extract were studied using HE-HPLC. Six chemicals in the A. scopariae extract, which could bind to hepatocytes in vitro, were detected by HPLC-DAD and three were identified as 7-hydroxy-coumarin (7-OH-C), capillartemisin A and 7-methoxy-coumarin, respectively. In vitro assays showed that 7-OH-C protected HL-7702 hepatocytes from H2O2 injury. The results indicated that these compounds could be extracted by hepatocytes, could be detected by HPLC and more importantly were bioactive. It is suggested that HE-HPLC is a useful method for screening potent active components in Chinese medicines used to treat liver diseases.
Ribosomal Protein S6 Kinase (RSK)-2 as a central effector molecule in RON receptor tyrosine kinase mediated epithelial to mesenchymal transition induced by macrophage-stimulating protein
Qi Ma, Sunny Guin, Snehal S Padhye, Yong-Qing Zhou, Rui-Wen Zhang, Ming-Hai Wang
Molecular Cancer , 2011, DOI: 10.1186/1476-4598-10-66
Abstract: The study presented here demonstrates that RSK2, a downstream signaling protein of the Ras-Erk1/2 pathway, is the principal molecule that links MSP-activated RON signaling to complete EMT. Using MDCK cells expressing RON as a model, a spindle-shape based screen was conducted, which identifies RSK2 among various intracellular proteins as a potential signaling molecule responsible for MSP-induced EMT. MSP stimulation dissociated RSK2 with Erk1/2 and promoted RSK2 nuclear translocation. MSP strongly induced RSK2 phosphorylation in a dose-dependent manner. These effects relied on RON and Erk1/2 phosphorylation, which is significantly potentiated by transforming growth factor (TGF)-β1, an EMT-inducing cytokine. Specific RSK inhibitor SL0101 completely prevented MSP-induced RSK phosphorylation, which results in inhibition of MSP-induced spindle-like morphology and suppression of cell migration associated with EMT. In HT-29 cancer cells that barely express RSK2, forced RSK2 expression results in EMT-like phenotype upon MSP stimulation. Moreover, specific siRNA-mediated silencing of RSK2 but not RSK1 in L3.6pl pancreatic cancer cells significantly inhibited MSP-induced EMT-like phenotype and cell migration.MSP-induced RSK2 activation is a critical determinant linking RON signaling to cellular EMT program. Inhibition of RSK2 activity may provide a therapeutic opportunity for blocking RON-mediated cancer cell migration and subsequent invasion.Epithelial to mesenchymal transition (EMT) is a biological process in polarized epithelial cells, which occurs in various physiological and pathological conditions [1]. Complete EMT is characterized by spindle-like cell morphology, loss of epithelial cellular markers such as E-cadherin, and gain of mesenchymal phenotype by expressing filament proteins including vimentin and α-smooth muscle actin [1,2]. Cells undergoing EMT are highly mobile and invasive [2,3]. During embryonic development, EMT enables cells to migrate or invade into neig
Effect of growth medium composition on the propagation of cultured milk vetch (Astragalus adsurgens Pall) roots and the allelopathic activity of extracts
培养基组分对沙打旺(Astragalus adsurgens Pall)组培根增殖的影响及其培养滤液提取物的化感活性

YU Fu-Ke,MA Yong-Qing,HUANG Xin-Hui,

生态学报 , 2008,
Abstract: The objective of this research was to determine effects of growth medium composition on the propagation of cultured milk vetch (Astragalus adsurgens Pall) roots. An orthogonal design was used to test multiple components in the growth medium. A second objective was to measure allelopathic activities of cultured milk vetch roots by conducting a bioassay using filtered extracts from the growth medium after culturing the roots. The results indicated that among all the nutritional components, Fe2+ had the greatest effect on the propagation of cultured milk vetch roots; the effects of sucrose,H2PO+4, Mg2+, Mn2+, Cu2+, Zn2+, BO3-3, Co2+, I- and C8H12ClNO3 + C12H18Cl2N4OS + C6H5O2N + C6H12O6 were intermediate; and the effects of nitrogen, Ca2+, MoO2-4 and NAA were lowest. A recommendation was made regarding the optimum nutrient content of growth medium for the propagation of cultured milk vetch roots. A bioassay using filtered extracts from the growth medium indicated that milk vetch roots might have produced allelopathic chemicals during their culture. Differences in the degree of allelopathic effects suggest that nutrient stress may influence the production of allelopathic chemicals. This study provides a basis for the improvement of milk root regeneration and propagation and suggests that nutrition may influence the production of allelopathic chemicals by this plant.
Application of allelopathy on the continuous cropping of cucumber in horticulture establishments

LI Ming,SHUI Jun-Feng,MA Yong-Qing,
李 明

中国生态农业学报 , 2006,
Abstract: Cucumber (Cucumis sativus L.) allelopathy plays an important role in the vegetable production. In this paper, the recent researches on cucumber allelopathy, allelopathic mode of action and the mechanism of allelochemicals inhibiting the continuous cropping cucumber growth, as well as the action between the allelopathy and the ecology system of horticulture establishments are reported. The prospects of cucumber allelopathy utilization in the cucumber plant under the horticulture establishments are discussed.
Collisional quantum interference on rotational energy transfer: Physical interpretation of the differential interference angle
Collisional quantum interference on rotational energy transfer: physical interpretation of the differential interference angle

Li Yong-Qing,Li Jian,Ma Feng-Cai,
,李 健,马凤才

中国物理 B , 2006,
Abstract: Collisional quantum interference (CQI) on the intramolecular rotational energy transfer is observed in an experiment with a static cell, and the integral interference angles are measured. To obtain more accurate information, an experiment with a molecular beam is carried out, and thereby the relationship between the differential interference angle and the scattering angle is obtained. Based on the first-Born approximation of time-dependent perturbation theory, the theoretical model of CQI is developed in an atom--diatom system in the condition of the molecular beam, with the long-range interaction potential taken into account. The method of measuring correctly the differential interference angle is presented. The tendencies of the differential interference angle changing with the impact parameter and relative velocity are discussed. The theoretical model presented here is important for understanding or performing the experiment in the molecular beam.
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