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Search Results: 1 - 10 of 27983 matches for " MA Wenhong "
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Obesity does not aggravate vitrification injury in mouse embryos: a prospective study
Ma Wenhong,Yang Xing,Liang Xiaoyan
Reproductive Biology and Endocrinology , 2012, DOI: 10.1186/1477-7827-10-68
Abstract: Background Obesity is associated with poor reproductive outcomes, but few reports have examined thawed embryo transfer in obese women. Many studies have shown that increased lipid accumulation aggravates vitrification injury in porcine and bovine embryos, but oocytes of these species have high lipid contents (63 ng and 161 ng, respectively). Almost nothing is known about lipids in human oocytes except that these cells are anecdotally known to be relatively lipid poor. In this regard, human oocytes are considered to be similar to those of the mouse, which contain approximately 4 ng total lipids/oocyte. To date, no available data show the impact of obesity on vitrification in mouse embryos. The aim of this study was to establish a murine model of maternal diet-induced obesity and to characterize the effect of obesity on vitrification by investigating the survival rate and embryo developmental competence after thawing. Methods Prospective comparisons were performed between six–eight-cell embryos from obese and normal-weight mice and between fresh and vitrified embryos. Female C57BL/6 mice were fed standard rodent chow (normal-weight group) or a high-fat diet (obese group) for 6 weeks. The mice were mated, zygotes were collected from oviducts and cultured for 3 days, and six–eight-cell embryos were then selected to assess lipid content in fresh embryos and to evaluate differences in apoptosis, survival, and development rates in response to vitrification. Results In fresh embryos from obese mice, the lipid content (0.044 vs 0.030, P<0.01) and apoptosis rate (15.1% vs.9.3%, P<0.05)were significantly higher, the survival rate (83.1% vs. 93.1%, P<0.01) on day 5 was significantly lower, and embryo development was notably delayed on days 3–5 compared with the normal-weight group. After vitrification, no significant difference was found between thawed embryos from obese and normal-weight mice in apoptosis, survival, and development rates on days 4 and 5. In both groups, pre- and post-vitrification embryo apoptosis, survival, and development rates were similar. Conclusions This study demonstrated that differences in survival and developmental rates between embryos from obese and normal-weight mice were eliminated after vitrification. Thus, maternal obesity does not aggravate vitrification injury, but obesity alone greatly impairs pre-implantation embryo survival and development.
Ecosystem carbon stocks and their changes in China’s grasslands
JingYun Fang,YuanHe Yang,WenHong Ma,Anwar Mohammat,HaiHua Shen
Science China Life Sciences , 2010, DOI: 10.1007/s11427-010-4029-x
Abstract: The knowledge of carbon (C) stock and its dynamics is crucial for understanding the role of grassland ecosystems in China’s terrestrial C cycle. To date, a comprehensive assessment on C balance in China’s grasslands is still lacking. By reviewing published literature, this study aims to evaluate ecosystem C stocks (both vegetation biomass and soil organic C) and their changes in China’s grasslands. Our results are summarized as follows: (1) biomass C density (C stock per area) of China’s grasslands differed greatly among previous studies, ranging from 215.8 to 348.1 g C m 2 with an average of 300.2 g C m 2. Likewise, soil C density also varied greatly between 8.5 and 15.1 kg C m 2. In total, ecosystem C stock in China’s grasslands was estimated at 29.1 Pg C. (2) Both the magnitude and direction of ecosystem C changes in China’s grasslands differed greatly among previous studies. According to recent reports, neither biomass nor soil C stock in China’s grasslands showed a significant change during the past 20 years, indicating that grassland ecosystems are C neutral. (3) Spatial patterns and temporal dynamics of grassland biomass were closely correlated with precipitation, while changes in soil C stocks exhibited close associations with soil moisture and soil texture. Human activities, such as livestock grazing and fencing could also affect ecosystem C dynamics in China’s grasslands.
Above- and belowground biomass in relation to environmental factors in temperate grasslands, Inner Mongolia
WenHong Ma,YuanHe Yang,JinSheng He,Hui Zeng,JingYun Fang
Science China Life Sciences , 2008, DOI: 10.1007/s11427-008-0029-5
Abstract: Above- and belowground biomasses of grasslands are important parameters for characterizing regional and global carbon cycles in grassland ecosystems. Compared with the relatively detailed information for aboveground biomass (AGB), belowground biomass (BGB) is poorly reported at the regional scales. The present study, based on a total of 113 sampling sites in temperate grassland of the Inner Mongolia, investigated regional distribution patterns of AGB, BGB, vertical distribution of roots, and their relationships with environmental factors. AGB and BGB increased from the southwest to the northeast of the study region. The largest biomass occurred in meadow steppe, with mean AGB and BGB of 196.7 and 1385.2 g/m2, respectively; while the lowest biomass occurred in desert steppe, with an AGB of 56.6 g/m2 and a BGB of 301.0 g/m2. In addition, about 47% of root biomass was distributed in the top 10 cm soil. Further statistical analysis indicated that precipitation was the primary determinant factor in shaping these distribution patterns. Vertical distribution of roots was significantly affected by precipitation, while the effects of soil texture and grassland types were weak.
Increasing terrestrial vegetation activity in China, 1982–1999
Jingyun Fang,Shilong Piao,Jinsheng He,Wenhong Ma
Science China Life Sciences , 2004, DOI: 10.1007/BF03182768
Abstract: Variations in vegetation activity during the past 18 years in China were investigated using the normalized difference vegetation index (NDVI) derived from the 3rd generation time series dataset of NOAA-AVHRR from 1982 to 1999. In order to eliminate the effects of non-vegetation factors, we characterized areas with NDVI < 0.1 as “sparsely vegetated areas” and areas with NDVI ≥0.1 as “vegetated areas”. The results showed that increasing NDVI trends were evident, to varying extents, in almost all regions in China in the 18 years, indicating that vegetation activity has been rising in recent years in these regions. Compared to the early 1980s, the vegetated area increased by 3.5% by the late 1990s, while the sparsely vegetated area declined by 18.1% in the same period. The national total mean annual NDVI increased by 7.4% during the study period. Extended growing seasons and increased plant growth rates accounted for the bulk of these increases, while increases in temperature and summer rainfall, and strengthening agricultural activity were also likely important factors. NDVI changes in China exhibited relatively large spatial heterogeneity; the eastern coastal regions experienced declining or indiscernibly rising trends, while agricultural regions and western China experienced marked increases. Such a pattern was due primarily to urbanization, agricultural activity, regional climate characteristics, and different vegetation responses to regional climate changes.
Biomass carbon stocks and their changes in northern China’s grasslands during 1982–2006
WenHong Ma,JingYun Fang,YuanHe Yang,Anwar Mohammat
Science China Life Sciences , 2010, DOI: 10.1007/s11427-010-4020-6
Abstract: Grassland covers approximately one-third of the area of China and plays an important role in the global terrestrial carbon (C) cycle. However, little is known about biomass C stocks and dynamics in these grasslands. During 2001–2005, we conducted five consecutive field sampling campaigns to investigate above-and below-ground biomass for northern China’s grasslands. Using measurements obtained from 341 sampling sites, together with a NDVI (normalized difference vegetation index) time series dataset over 1982–2006, we examined changes in biomass C stock during the past 25 years. Our results showed that biomass C stock in northern China’s grasslands was estimated at 557.5 Tg C (1 Tg=1012 g), with a mean density of 39.5 g C m 2 for above-ground biomass and 244.6 g C m 2 for below-ground biomass. An increasing rate of 0.2 Tg C yr 1 has been observed over the past 25 years, but grassland biomass has not experienced a significant change since the late 1980s. Seasonal rainfall (January–July) was the dominant factor driving temporal dynamics in biomass C stock; however, the responses of grassland biomass to climate variables differed among various grassland types. Biomass in arid grasslands (i.e., desert steppe and typical steppe) was significantly associated with precipitation, while biomass in humid grasslands (i.e., alpine meadow) was positively correlated with mean January-July temperatures. These results suggest that different grassland ecosystems in China may show diverse responses to future climate changes.
Increasing terrestrial vegetation activity in China, 1982-1999
Increasing terrestrial vegetation activity in China, 1982—1999

FANG Jingyun,PIAO Shilong,HE Jinsheng,MA Wenhong,

中国科学C辑(英文版) , 2004,
Abstract: Variations in vegetation activity during the past 18 years in China were investigated using the normalized difference vegetation index (NDVI) derived from the 3rd generation time series dataset of NOAA-AVHRR from 1982 to 1999. In order to eliminate the effects of non-vegetation factors, we characterized areas with NDVI < 0.1 as "sparsely vegetated areas" and areas with NDVI > or = 0.1 as "vegetated areas". The results showed that increasing NDVI trends were evident, to varying extents, in almost all regions in China in the 18 years, indicating that vegetation activity has been rising in recent years in these regions. Compared to the early 1980s, the vegetated area increased by 3.5% by the late 1990s, while the sparsely vegetated area declined by 18.1% in the same period. The national total mean annual NDVI increased by 7.4% during the study period. Extended growing seasons and increased plant growth rates accounted for the bulk of these increases, while increases in temperature and summer rainfall, and strengthening agricultural activity were also likely important factors. NDVI changes in China exhibited relatively large spatial heterogeneity; the eastern coastal regions experienced declining or indiscernibly rising trends, while agricultural regions and western China experienced marked increases. Such a pattern was due primarily to urbanization, agricultural activity, regional climate characteristics, and different vegetation responses to regional climate changes.
Enhanced Energy-Aware Feedback Scheduling of Embedded Control Systems
Feng Xia,Longhua Ma,Wenhong Zhao,Youxian Sun
Journal of Computers , 2009, DOI: 10.4304/jcp.4.2.103-111
Abstract: Dynamic voltage scaling (DVS) is one of the most effective techniques for reducing energy consumption in embedded and real-time systems. However, traditional DVS algorithms have inherent limitations on their capability in energy saving since they rarely take into account the actual application requirements and often exploit fixed timing constraints of real-time tasks. Taking advantage of application adaptation, an enhanced energy-aware feedback scheduling (EEAFS) scheme is proposed, which integrates feedback scheduling with DVS. To achieve further reduction in energy consumption over pure DVS while not jeopardizing the quality of control, the sampling period of each control loop is adapted to its actual control performance, thus exploring flexible timing constraints on control tasks. Extensive simulation results are given to demonstrate the effectiveness of EEAFS under different scenarios. Compared with the optimal pure DVS scheme, EEAFS saves much more energy while yielding comparable control performance.
Enhanced Energy-Aware Feedback Scheduling of Embedded Control Systems
Feng Xia,Longhua Ma,Wenhong Zhao,Youxian Sun,Jinxiang Dong
Computer Science , 2008,
Abstract: Dynamic voltage scaling (DVS) is one of the most effective techniques for reducing energy consumption in embedded and real-time systems. However, traditional DVS algorithms have inherent limitations on their capability in energy saving since they rarely take into account the actual application requirements and often exploit fixed timing constraints of real-time tasks. Taking advantage of application adaptation, an enhanced energy-aware feedback scheduling (EEAFS) scheme is proposed, which integrates feedback scheduling with DVS. To achieve further reduction in energy consumption over pure DVS while not jeopardizing the quality of control, the sampling period of each control loop is adapted to its actual control performance, thus exploring flexible timing constraints on control tasks. Extensive simulation results are given to demonstrate the effectiveness of EEAFS under different scenarios. Compared with the optimal pure DVS scheme, EEAFS saves much more energy while yielding comparable control performance.
Notch Signaling Mediates TNF-α-Induced IL-6 Production in Cultured Fibroblast-Like Synoviocytes from Rheumatoid Arthritis
Zhijun Jiao,Wenhong Wang,Jie Ma,Shengjun Wang,Zhaoliang Su,Huaxi Xu
Clinical and Developmental Immunology , 2012, DOI: 10.1155/2012/350209
Abstract: It has been reported that Notch family proteins are expressed in synovium tissue and involved in the proliferation of synoviocyte from rheumatoid arthritis (RA). The aim of this paper was to investigate whether Notch signaling mediated TNF-α-induced cytokine production of cultured fibroblast-like synoviocytes (FLSs) from RA. Exposure of RA FLSs to TNF-α (10 ng/ml) led to increase of Hes-1, a target gene of Notch signaling, and a marked upregulation of Notch 2, Delta-like 1, and Delta-like 3 mRNA levels. Blockage of Notch signaling by a γ-secretase inhibitor (DAPT) inhibited IL-6 secretion of RA FLSs in response to TNF-α while treatment with recombinant fusion protein of Notch ligand Delta-like 1 promoted such response. TNF-α stimulation also induced IL-6 secretion in OA FLSs; however, the Hes-1 level remained unaffected. Our data confirm the functional involvement of Notch pathway in the pathophysiology of RA FLSs which may provide a new target for RA therapy.
Optimization of Glutamine Peptide Production from Soybean Meal and Analysis of Molecular Weight Distribution of Hydrolysates
Yanli Xie,Xinhong Liang,Min Wei,Wenhong Zhao,Baoshan He,Qiyu Lu,Quangong Huo,Chengye Ma
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13067483
Abstract: The process parameters of enzymatic hydrolysis and molecular weight distribution of glutamine (Gln) peptides from soybean meal were investigated. The Protamex ? hydrolysis pH of 6.10, temperature of 56.78 °C, enzyme to substrate ratio (E/S) of 1.90 and hydrolysis time of 10.72 h were found to be the optimal conditions by response surface methodology (RSM) for a maximal degree of hydrolysis (DH) value of 16.63% and Gln peptides content at 5.95 mmol/L. The soybean meal was hydrolyzed by a combination of Protamex ? and trypsinase so that DH and Gln peptides would reach 22.02% and 6.05 mmol/mL, respectively. The results of size exclusion chromatography indicated that the relative proportion of the molecular weight < 1000 Da fraction increased with DH values from 6.76%, 11.13%, 17.89% to 22.02%, most notably the 132–500 Da fractions of hydrolysates were 42.14%, 46.57%, 58.44% and 69.65%. High DH values did not lead to high Gln peptides content of the hydrolysate but to the low molecular weight Gln peptides.
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