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Search Results: 1 - 10 of 482137 matches for " M.X. Chen "
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S.P. Li,S.X. Zhao,M.X Pan,D.Q. Zhao,and X.C.Chen
金属学报(英文版) , 1996,
Characterisation of Angiostrongylus cantonensis Isolates from China by Sequences of Internal Transcribed Spacers of Nuclear Ribosomal DNA
C.Y. Liu,R.L. Zhang,M.X. Chen,J. Li,L. Ai,C.Y. Wu,X.Q. Zhu,R.Q. Lin
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.593.596
Abstract: The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among Angiostrongylus cantonensis isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual A. cantonensis by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese A. cantonensis specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in A. cantonensis was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between A. cantonensis and other Angiostrongylus sp. The results showed that the ITS sequences were conserved among the A. cantonensis isolates however, they were quite different from that of other Angiostrongylus species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of Angiostrongylus sp.
Sequences of Internal Transcribed Spacers and Two Mitochondrial Genes: Effective Genetic Markers for Metorchis orientalis
L. Ai,S.H. Chen,Y.N. Zhang,X.N. Zhou,H. Li,M.X. Chen,J. Guo,Y.C. Cai,X.Q. Zhu,J.X. Chen
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.2371.2376
Abstract: The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and two mitochondrial DNA (mtDNA) regions, namely cytochromec oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 (nad1), among Metorchis orientalis metacercaria isolates from Guangxi in China. The sequences of ITS, pcox1 and pnad1 were amplified from 6 individual M. orientalis metacercariae and sequenced. The relevant sequences of other 7 trematode species belonging to 6 genera in 4 families were downloaded from GenBank and their phylogenetic relationships were re-constructed using the combined pcox1 and pnad1 mt DNA sequences with Trichinella spiralis as outgroup. The results showed that sequences of ITS rDNA, pcox1 and pnad1 of M. orientalis were 1131, 654 and 650 bp, respectively and they were quite conserved among the M. orientalis isolates. However, they were quite different from that of other species, phylogenetic analysis of the combined pcox1 and pnad1 mt DNA sequences were able to distinguish M. orientalis from different species of the Opisthorchiidae and trematodes in other families. Therefore, the ITS, cox1 and nad1 mt DNA sequences provide effective genetic markers for the specific identification of trematodes of the Opisthorchiidae family and have implications for studying their population biology, genetic structure, as well as molecular epidemiology.
Prevalence of Anisakid Larvae in Marine Fishes Sold in Shenzhen, China
M.X. Chen,J.X. Chen,Y.G. Ye,S.T. Gao,Y.J. Geng,D.N. Huang,X.H. Li,R.L. Zhang,X.Q. Zhu,M.J. Xu
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.1816.1818
Abstract: The aim of the present investigation was to examine the prevalence of anisakid larvae in marine fishes sold in Shenzhen in March to November 2008. A total of 394 individual fishes representing 40 fish species were obtained from different wholesale markets. They were examined for the presence of anisakid larvae by examination of the viscera and whole body cavity. About 23 species of fish (57.5%, 23/40) namely Decapterus maruadsi, Harpodon nehereus, Muraenesox cinereus, Trichiurus haumela, Pneumatophorus japonicus, Sebastods schlegeli and so on were found to be infected with anisakid larvae with an overall infection rate of 31.0% (122/394). The results of present investigation demonstrated the risk of human infection with anisakid larvae in Shenzhen, China which have implications for implementing control strategies against anisakid infection in humans in China.
Characterization of MicroRNAs in Paragonimus westermani by Solexa Deep Sequencing and Bioinformatics Analysis
L. Ai,M.X. Chen,S.H. Chen,Y.N. Zhang,H. Li,Y.C. Cai,Y. Lu,L.G. Tian,X.N. Zhou,J.X. Chen
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.3469.3473
Abstract: MicroRNAs (miRNAs) are small non-coding RNA which generate from large hairpin precursors and play important role on post-transcriptional regulators of target genes. Although, many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in trematode. By the method of using high throughput Solexa sequencing technology, 11.02 million clean reads have been obtained of Paragonimus westermani. Among the clean reads, 1.14 million ones (10.37%) were perfectly mapped onto the S. japonicum genome which included 20,322 (0.69%) unique sequences. A number of 9,492,613 (86.17%) reads had no match with public databases and marked as un-annotated RNAs. Nucleotide bias analysis found that the known miRNAs showed high bias and the guanine was the dominant nucleotide, particularly at the 2nd and 23rd positions which were almost at the beginning and end of conserved miRNAs. Three novel miRNA corresponding to 18 precursors were predicted. To the knowledge, this is the first report of miRNA profiles in P. westermani which will contribute to better understanding of the complex biology of this zoonotic parasite. The reported data of P. westermani miRNAs should provide valuable references for miRNA studies of closed related zoonotic trematode.
Prevalence of Paragonimus and Angiostrongylus cantonensis Infections in Snails in Southeastern China
Y.Z. Cheng,J. Hou,X.H. He,Z.K. Hong,L.S. Li,G.H. Lin,M.X. Chen,S.H. Chen
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.2599.2602
Abstract: Paragonimiasis and angiostrongyliasis are important food-borne parasitic diseases in a number of countries including China. Both diseases are transmitted by freshwater and terrestrial intermediate snail hosts. In the present investigation, we examined the prevalence of Paragonimus and Angiostrongylus cantonensis in the intermediate host snails in southeastern China s Fujian province where paragonimiasis and angiostrongyliasis are endemic. The prevalence of P. westermani cercariae in the stream-type snail Semisulcospira libertine found in the breeding grounds in Shouning county was 0.09% in the upstream and 0.56% in the downstream, respectively. For pit-ditch type, the prevalence of P. westermani cercariae was 0.19% and 0.82% in upstream and downstream, respectively. The snail Tricula xiaoqiaoensis was infected with Paragonimus skrjabini cercariae with prevalence of 0.33% in the pit-ditch type and 1.52% in the seepage type in Zhenghe county. Prevalence of A. cantonensis larvae in Achatina fulica in Nan an county in garbage stack, vegetable plot and crop land were 83.54, 39.53 and 19.23%, respectively. In Lianjiang county, prevalence of A. cantonensis larvae in snails Pila polita in trench, paddy field, residential ditch, vegetable irrigation ditch and pond in the same river system were 4.08, 8.82, 75.34, 34.04 and 5.56%, respectively. The snail Bellamya lithophaga found in the western suburb of Fuzhou city was identified as the new intermediated host for A. cantonensis with a prevalence of 14.38%. Demonstration of prevalence of Paragonimus and A. cantonensis in wild snails in Fujian province poses substantial risk for future outbreaks of the two food-borne parasitic diseases.
The role of the principal, teachers and students in restoring the culture of learning, teaching and service (COLT) in black secondary schools in the Pretoria region
M.X. Lethoko, J. Heystek, J.G. Maree
South African Journal of Education , 2001,
Abstract: Political and social instability over many years has led to a crisis in the South African education system. One of the crucial changes and challenges that the new democracy faces is to reconstruct a society and an education system that will be able to establish a culture conducive to learning, teaching and services in schools (COLT). This article examines factors that have led to a loss of the culture of learning and teaching (COLT) in South African schools, and the characteristics of a positive culture expected by principals, teachers and students. Results indicate that although principals are attempting to influence this culture, there is little support for such changes from teachers and students. (South African Journal of Education: 2001 21(4): 311-316)
Prevalência de mineraliza??o aórtica detectada no abate de bovinos no estado de Minas Gerais, Brasil
Silva, M.X.S.;Guedes, R.M.C.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2007, DOI: 10.1590/S0102-09352007000300002
Abstract: this study describes the prevalence of aortic mineralization in slaughtered bovine in the state of minas gerais. fragments of thoracic aorta from 783 animals from different origin, breed, gender and age were evaluated macroscopically. all lesions were characterized and divided in, grade i, grade ii, grade iii and grade iv, depending on the severity of the lesions. a total of 298 (38.1%) aortas had lesions, as follow: 180 grade i (23.0%), 59 grade ii (7.5%), 35 grade iii (4.5%) and 24 grade iv (3.1%). differences concerning age, breed and sex were observed. females and castrated males (54.6% and 50.7%, p<0.00001 and p<0.0001, respectively) compared to intact males, zebu-crossbred and mixed bred (59.3% and 47.8%, p<0.02 and p<0.05, respectively) compared to pure zebu (37,4%) and animals older then 3 years (59.4%, p<0.0001) compared to animals 2-3 years or under 2 years of age (21.7% and 13.9%, respectively) were more frequently affected by aortic mineralization.
A amiodarona e a tireóide
Pavan, Renata;Jesus, Alexandre M.X. de;Maciel, Léa M.Z.;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2004, DOI: 10.1590/S0004-27302004000100020
Abstract: amiodarone is an iodine-rich drug widely used for the management of cardiac arrhythmias. during long-term amiodarone therapy drug toxicity may occur and a substantial proportion of amiodarone-treated patients develop either hypothyroidism or thyrotoxicosis. several mechanisms are involved in amiodarone-induced thyroid dysfunction: defective thyroid auto-regulatory mechanism in presence of excessive iodine offer, immunological factors and cytotoxicity. approximately 50% of patients taking amiodarone present abnormal thyroid function. therefore, for adequate clinical follow up of these patients, it is critical a careful monitoring of thyroid hormones and tsh, before and during treatment.
Node Distribution in Wireless Sensor Networks Using Component Based Localization Algorithm
M.X.Aquien,,T. Sudarson Rama Perumal, G.Kharmega Sundararaj
International Journal of Computer Technology and Electronics Engineering , 2012,
Abstract: Localization is crucial for wireless ad hoc and sensor networks. As the distance-measurement ranges are often less than the communication ranges for many ranging systems, most communication-dense wireless networks are localization-sparse. Consequently, existing algorithms fail to provide accurate localization supports. In order to address this issue, by introducing the concept of component, we group nodes into components so that nodes are able to better share ranging and anchor knowledge. Operating on the granularity of components, our design, CALL, relaxes two essential restrictions in localization: the node ordering and the anchor distribution. Compared to previous designs, CALL is proven to be able to locate the same number of nodes using the least information
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