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Search Results: 1 - 10 of 718022 matches for " M.A. Abol Hassan "
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Comparison of Binding Capacity and Affinity of Monoclonal Antibody towards Different Affinity Resins using High-throughput Chromatography Method
N. Pakiman,N.H. Isa,M.A. Abol Hassan,J.K. Walter
Journal of Applied Sciences , 2012,
Abstract: Protein-A Affinity chromatography is the widely used key method for purification of monoclonal antibodies. Selection of a most suitable affinity resin based on binding capacity and affinity is typically performed prior to optimization. Development of high-throughput chromatography method in 96-well filter plate significantly reduced consumption of antibody sample and shortens the experimental time as compared to a typical column chromatography approach. In this study, five different affinity resins were evaluated, rProtein-A FF, MabSelect Sure, ProSep-vA Ultra and two novel synthetically derived affinity ligands immobilized on agarose media, the GF1 and GF2 resins. Resins were dispensed on a 96-well filter plate and antibody sample with different protein concentration was loaded to evaluate resins affinity and static binding capacity. MabSelect Sure, an agarose based matrix with alkaline resistance Protein-A ligand and ProSep-vA Ultra that is a rigid pore glass resin exhibit the highest static binding capacity at ~60-63 mg IgG mL-1 of resin. The two novel resins, GF1 and GF2 show moderate binding capacity at ~28-34 mg IgG mL-1 of resin. By addition of salts during binding, the capacity of the novel resins was enhanced to ~33-42 mg IgG mL-1 resin. Affinity of all evaluated resins was quite comparable. Few other factors for resin selection such as dynamic binding capacity, ligand stability and resistance including resin cost will be briefly discussed.
Nutritional Status and Susceptibility of Advanced Chickpea Germplasm to Low Soil Zinc and Boron
M.A.Kausar,M.Sadiq,M.A.Khan,M. Hassan
Pakistan Journal of Biological Sciences , 2000,
Abstract: Shoot samples at preflowering stage and grains at maturity from four replicated field trials on advanced germplasm of chickpea along with some of the commercial varieties were collected. Three trials consisted of Desi germplasm with 12, 12, and 8 entries while the fourth one had 11 entries of Kabuli material. The advanced lines appeared to be highly promising as they gave maximum increase over the old check varieties in grain yield upto 60 percent in first trial followed by 58 percent in second, 53 percent in third and 43 percent in the 4th one. On the overall basis, the whole germplasm under study contained sufficient B and Cu, while Zn and P (in grain) were marginal if not deficient. Almost all the material had relatively higher B in the grain than that of cereals like wheat. After field survey, a followup soil pot culture study was conducted employing 5 varieties and 2 lines on a soil with marginal Zn and B. Variety 6153 responded to 5 mg kg -1 Zn application by 39, CM 72 by 26, NIFA 95 by 17 and mutant CM 1571-1-A by 11 percent, while remaining responded negligibly. Mutant CM 31-1 responded to 1 mg kg -1 B application by 30, CM 1571-1-A, C 44 and 6153 shared the response by 27 percent. Remaining three did not respond or responded negatively. All the entries in the four trials contained marginal Zn i.e.,<20 mg kg-1 and sufficient B i.e., >30 mg kg-1, yet some of them responded to the applications markedly indicating their high B and Zn requirement than reported in the literature.
Soil Water Management and Conservation Practices Towards a New Cropping Pattern in Drought Prone Areas of Bangladesh
A.A. Hassan,N.N. Karim,M.A. Hamid,M.A. Salam
Journal of Agronomy , 2003,
Abstract: Experiment was conducted to increase cropping intensity through soil water management and conservation practices. Transplanted rainfed aman rice is grown in Cropping patterns studied during 2000-01 were rice-legume-legume and rice-fallow-fallow, using early maturing drought tolerant crop varieties and local rice. Tillage treatments were imposed during land preparation. Mulch treatments were also imposed in legume rotations. Newly introduced varieties, BRRIdhan 33 and BINAdhan 4, were earlier by 25 and 15 days, respectively, compared with the local rice Sarna. The early harvest of rice varieties left enough residual moisture due to tillage amendments and mulch applications. The available profile soil moisture left was able to meet up about two-third of the water requirement of chickpea (cv. BINAsola2 and Hyprosola). Pre-sowing irrigation, tillage, mulch practices and little rain contributed to successful production of mungbean cv. BINAmoog 2 after chickpea. A reasonable yield of all the crops was obtained compared with the national average yield. The introduction of chickpea and mungbean in the cropping pattern resulted the cropping intensity from 100 to 300%. The altered cropping pattern (rice-legume-legume) evaluated with the existing one (rice-fallow-fallow) resulted the net return of t 37144.00 to t 55406.00 with BCR value of 1.94-2.40. The achievement of a new cropping pattern was only possible due to introduction of early drought tolerant crop varieties, soil water management and conservation practices.
Influence of urea-molasses treated wheat straw fermented with cattle manure on nutrient intake, digestibilities, milk yield and its composition in early lactating Nili Ravi buffaloes
M. Nisa,M. Sarwar,M.A. Shahzad,Z. Hassan
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2007.s2.480
Abstract: This study was aimed to examine the influence of urea-molasses treated wheat straw (WS) fermented with cattle manure (CM) with 4% urea and 4% molasses incubated for 40 days on its chemical composition and varying substitution levels of fermented wheat straw (FWS) with concentrate on nutrients intake and their digestibilities, milk yield and its composition in Nili-Ravi buffaloes. Twenty early lactating buffaloes were randomly divided into four groups in a randomized complete block design. Four iso-nitrogen and iso-caloric diets were formulated. There was no replacement of concentrate with FWS in control (FWS 0) diet while FWS 15, FWS 25 and FWS 35 dies contained 15, 25 and 35 % replacement of concentrate with FWS, respectively. Nutrients intake remained unaltered (P>0.05) by lactating buffaloes fed diets containing varying levels of FWS. However, neutral detergent fiber and acid detergent fiber digestibilities increased (P<0.05) in buffaloes fed FWS20 and FWS30 diets than those fed FWS10 and FWS0 diets. Milk yield (4% fat corrected milk) also remained unaltered. This study implied that urea molasses treated WS fermented with CM replaced 30% dietary concentrate without affecting the milk yield and its quality by early lactating buffaloes.
A Preliminary Investigation of Compressed Producer Gas from Downdraft Biomass Gasifier
S. Hassan,Z.A. Zainal,M.A. Miskam
Journal of Applied Sciences , 2010,
Abstract: The developmental work on compressing of producer gas from downdraft gasifier using a single stage normal air compressor has been studied and evaluated. Producer gas, which generated from the downdraft gasification process is basically a combustible gas which can be used for heating purposes and as an alternative fuel to generate power in an Internal Combustion (IC) engine. In the current practice, the producer is used directly from downdraft gasifier mixed with air through a carburetor, mixer or simple T-joint before entering the cylinder of an IC engine. The producer gas has to be consistently generated and supplied to the engine and any shortcomings or unstable gas from the gasifier will affect the quantity and quality of the gas required. In this study, air compressor was used to induce the producer gas from the gasifier at 670 L min-1, compressed it to 7.6 bar gauge pressure and discharged it to a specified flow rate. The discharged flow rate of the producer gas was regulated at 130, 150 and 170 L min-1 consistently at an output pressure of 2.0, 2.5 and 3.0 bar, respectively. It was found that the blue flame producer gas was able to flare continuously through two outlets of producer gas ports after the gasifier and one outlet of producer gas port after the air compressor. The discharged flow rate of producer gas was constant over the entire range set of pressures. Since, the output pressure was regulated higher than the atmospheric, the density of the producer gas proportionally increased with the increase in pressure and the gas flow rate supplied can be controlled to the desired consumption particularly in the IC engines.
Apparent Digestibility Coefficients of Pelleted Feed Incorporated with Water Hyacinth Echhornia crassipes Fed to Red Tilapia, [Oreochromis mossambicus (Peters, 1852) X Oreochromis niloticus (Linnaeus, 1758)]
E.A.T. Mubarak,M.A. Amiza,H.K. Bakhsh,A.B. Abol-Munafi
Agricultural Journal , 2013, DOI: 10.3923/aj.2011.322.326
Abstract: A 3 weeks feeding trial was conducted to evaluate the Apparent Digestibility Coefficients (ADCs) of dry matter, protein, gross energy, nitrogen, crude lipid and fiber of Water Hyacinth (WH), Echhornia crassipes meal incorporated in pelleted test feeds (0, 10, 15, 20 and 25%, dry matter basis) for red tilapia fingerlings. Chromium dioxide (1%) was added as an inert bio-marker to iso-nitrogenous (35.00±0.20% crude protein) and iso-caloric (4.00±0.52 kcal kg-1) feeds. Results showed that the maximum value (p = 0.05) of ADCs for dry matter (68.09%) was recorded for the control feed with 0% WH while the minimum value of 50.36% was recorded for the test feed 4 including 20% WH. Similarly, the maximum ADCs values (p = 0.05) for crude protein, gross energy, ether extract, crude lipid and crude fiber were also found in control feed while the minimum values were observed in test feed 4 including 20% WH. The study was clearly indicated that red tilapia fingerlings efficiently digest the nutrients when only the maximum inclusion of WH 20% in their feed does not exceed 20% of WH meal (on dry basis).
African Journal of Urology , 2002,
Abstract: Objective To determine the incidence of prostatic adenocarcinoma in bilharzial patients who previously underwent radical cystoprostatectomy for bladder tumors. Patients and Methods From February 1997 to February 1999, 249 male patients with bladder cancer were screened for prostate cancer prior to cystectomy using DRE and total PSA assay, as well as transrectal ultrasound-guided prostatic biopsies. Then the cystoprostatectomy specimens were serially sectioned (every 3 mm) and histologically examined. Results Prostatic adenocarcinoma was detected by ultrasound-guided prostatic needle biopsies in 2 cases, while in 18 it was discovered incidentally after cystoprostatectomy (total 20 patients = 8%). Gleason score was 6 in 16 patients, 7 in 3 patients and 8 in the remaining patient. Perineural lymphatic permeation was observed in 4 cases and extracapsular extension in one. Conclusion Compared to previous reports on non-bilharzial patients, the incidence of prostate cancer in the cystoprostatectomy specimens of bilharzial patients was low, and the tumors were clinically insignificant in most of the cases. Adénocarcinome Prostatique dans les Pièces de Cysto-Prostatectomie pour Tumeurs de Vessie Bilharzienne Objectif Déterminer lincidence de ladénocarcinome prostatique chez des patients bilharziens qui ont subi une cysto-prostatectomie radicale pour tumeur de vessie. Patients et Méthodes De Février 1997 à Février 1999, 249 patients de sexe masculin ont bénéficié dun dépistage de cancer prostatique avant une cystectomie. Le dépistage a consisté en un toucher rectal, un dosage du taux de PSA total et des biopsies prostatiques guidées par échographie trans-rectale. Puis des coupes sériées tous les 3 mm ont été pratiquées sur les pièces de cysto-prostatectomie suivies dun examen histologique. Résultats Un adénocarcinome prostatique a été détecté chez deux patients avec les biopsies écho-guidées, tandis que 18 cas ont été retrouvés fortuitement sur les pièces de cysto-prostatectomie (total 20 patients = 8%). Le score de Gleason était de 6 chez 16 patients, 7 chez 3 patients et 8 chez 1 patient. Une perméation lymphatique péri-nerveuse a été observée chez 4 patients et une extension capsulaire chez un patient. Conclusion Comparée aux études antérieures portant sur des patients non bilharziens, lincidence du cancer de la prostate dans les pièces de cysto-prostatectomie chez les patients bilharziens est très faible. De plus, les tumeurs sont cliniquement insignifiantes dans la plupart des cas. African Journal of Urology Vol.8(2) 2002: 87-93
Heat transfer characteristics of R410A during its evaporation inside horizontal tube
M. Fatouh, A.B. Helali, M.A.M. Hassan, A. Abdala
International Journal of Energy and Environment , 2011,
Abstract: Evaporative local heat transfer coefficients of R22 and R410A are determined in electrically heated a smooth horizontal copper tube with inner diameter of 9.525 mm and length of 1000 mm. Experiments are carried out varying: the vapor quality (0.1:1), the heat fluxes (10:29) kW/m2 and the evaporation temperatures (-5:5?C) at mass flux of 100 kg/m2s. Effect of the above operating parameters on heat transfer coefficients are investigated and reported in graphical forms. Experimental results showed that the heat transfer coefficients of R410A are better than those of R22, by about 17% and 14% during the lower and higher value of evaporating pressures and heat flux, respectively. The modified Kattan model results were found to be in much better agreement with the experimental results than the correlations from Gungor [12], Shah [20] and other correlations.
Responses of the Mullet, Liza auratus and the Cichlid, Oreochromis niloticus from Lake Manzala (Egypt) to Heterophyd Infection
M.A. Ghobashy,M.F.M. Soliman,E.A. Hassan
International Journal of Zoological Research , 2010,
Abstract: In this study, the occurrence of heterophyid infection in two well-known hosts of heterophyd in Egyptian lake (Manzala); the mullet, Liza auratus and the cichlid, Oreochromis niloticus was investigated. Furthermore, the potential factors that possibly affect the occurrence of the infection including host sex, length, weight and seasonal variation were considered. The pathological response of the two fish host to the infection was studied. Results showed that the prevalence, abundance and intensity of infection in the two fish host greatly affected by the factor considered in contradictory way. The responses to infection and the possible effect of the interaction between all the considered factors are discussed in details. In addition, metacercarial infection caused alterations in the histological architecture of the infected tissues and in the composition of the muscle proteins as well which was more pronounced in O. niloticus and L. auratus, respectively. In conclusion, many biological and environmental factors do affect the occurrence of heterophyid infection in addition to the anthropogenic activity. L. auratus was more susceptible to the infection as compared to O. niloticus from the same habitat.
Genetic Evaluation and Selection Criteria of Hybrid Rice in Irrigated Ecosystem of Bangladesh
K.M. Iftekharuddaula,M.S. Hassan,M.J. Islam,M.A. Badshah
Pakistan Journal of Biological Sciences , 2001,
Abstract: Nineteen-hybrid rice were evaluated in order to determine variability and genetic association for grain yield and its component characters. It was observed that all the tested characters had significant variation. The highest genotypic coefficient of variation was found in panicles per m2, followed by spikelets per panicles, grain yield, 1000-grain weight and flag leaf area. High heritability was observed for all the tested characters except grains per panicle and harvest index. High heritability and genetic advance was found in panicles per m2, spikelets per panicle and 1000-grain weight. Genotypic correlations were higher than the phenotypic correlations in most of the cases. Flag leaf area, days to maturity, grains per panicle, panicles per m2, , 1000-grain weight and harvest index showed highly significant positive correlation with grain yield both at genotypic and phenotypic levels. Path coefficient analysis of the study revealed that higher harvest index, adequate spikelets per panicle, days to maturity, more panicles per m2, and heavy grains had direct effect on grain yield. Grains per panicle had a positive but indirect effect on grain yield through spikelets per panicles and harvest index. Similar trends were also observed in panicle length through spikelets per panicle and in flag leaf area through harvest index.
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