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Search Results: 1 - 10 of 672195 matches for " M. de L. Capurro "
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Primary characterization and basal promoter activity of two hexamerin genes of Musca domestica
C.K. Moreira,M. de L. Capurro,M. Walter,E. Pavlova
Journal of Insect Science , 2004,
Abstract: Hexamerins are high molecular-weight proteins found in the hemolymph of insects and have been proposed to function as storage proteins. In previous studies, two Musca domestica hexamerins, designated Hex-L and Hex-F were characterized. Hex-L is synthesized exclusively by the larval fat bodies, is secreted into the hemolymph and likely provides a source of amino acids and energy during metamorphosis. Hex-F synthesis is induced by a proteinaceous meal and occurs only in the adult insect fat bodies. Hex-F also is secreted into the hemolymph and it has been suggested that in females it may be an amino acid reservoir to be used during the final stages of egg formation. Genomic clones containing full-length copies of the genes MdHexL1 and MdHexF1, encoding subunits of the larval and the adult female hexamerin, respectively, were isolated. Complete nucleotide sequences, including the 5'-end untranscribed regions, were determined and analyzed for each of the genes. Comparisons of the conceptual translation products of the cloned genes indicated that MdHexL1 and MdHexF1 are related to the larval serum proteins (LSP) 1 and 2 of Calliphora vicina and Drosophila melanogaster. DNA fragments containing the putative promoters of the two hexamerin genes were compared and cloned into a plasmid vector so as to drive the expression of the GFP reporter gene. The constructs were assayed in vitro in transfected S2 Drosophila melanogaster cells demonstrating that the cloned M. domestica DNA fragments exhibit promoter activity.
Tissue distribution and lipophorin transport of hydrocarbons and sex pheromones in the house fly, Musca domestica
Coby Schal,Veeresh Sevala,Margareth de L. Capurro,Theodore E. Snyder
Journal of Insect Science , 2001,
Abstract: We investigated the relationship between epicuticular and internal hydrocarbons in the adult house fly, Musca domestica and the distribution of hydrocarbons, including the female sex pheromone component, (Z)-9-tricosene, in tissues. Internal hydrocarbons increased dramatically in relation to sexual maturation and were found in the hemolymph, ovaries, digestive tract, and fat body. (Z)-9-Tricosene comprised a relatively large fraction of the hydrocarbons in the female carcass and hemolymph, and less so in other tissues, while other hydrocarbons were represented in greater amounts in the ovaries than in other tissues. It therefore appears that certain hydrocarbons were selectively provisioned to certain tissues such as the ovaries, from which pheromone was relatively excluded. Both KBr gradient ultracentrifugation and specific immunoprecipitation indicated that > 90% of hemolymph hydrocarbons were associated with a high-density lipophorin (density = 1.09 g ml-1), composed of two apoproteins under denaturing conditions, apolipophorin I (~240 kD) and apolipophorin II (~85 kD). Our results support a predicted model (Chino, 1985) that lipophorin is involved in the transport of sex pheromone in M. domestica. In addition to delivering hydrocarbons and sex pheromones to the cuticular surface, we suggest that lipophorin may play an important role in an active mechanism that selectively deposits certain subsets of hydrocarbons at specific tissues.
Resultados de profilaxis con itraconazol 800 mg/día vía oral en adultos con leucemia aguda y neutropenia de alto riesgo: Hospital del Salvador 2006-2008
Andrade M,Alejandro; Puga L,Barbara; Guerra C,Carolina; Molina E,Javiera; Capurro C,Marisa;
Revista médica de Chile , 2011, DOI: 10.4067/S0034-98872011000900003
Abstract: background: systemic fungal infections and specifically invasive aspergillosis (ia) are associated with a high morbi-mortality rate in patients with hematologic malignancies. itraconazole kinetic studies show that plasma levels are not satisfactory, even though there is a reduction of the severity in clinical cases. aim: to evaluate the results of oral prophylaxis with high dose itraconazole, 400 mg bid, among patients with adult acute leukemia. material and methods: prospective analysis of 93 high risk febrile episodes (with an absolute neutrophil count of less than 500 x mm3 for more 10 days), that occurred in 76 patients. results: seventy five percent of episodes occurred in patients with acute myeloid leukemia and 25% in patients with acute lymphoblastic leukemia. fifty two percent occurred during the induction of chemotherapy. median duration of severe neutropenia was 21 days (range 10-48). median duration of itraconazole prophylaxis was 17 days (range 6-34). a low frequency of invasive fungal infections was observed (17%). according to diagnostic criteria, 5% of episodes corresponded to persistent fever , 1% and 11% of episodes, to probable or possible ia, respectively. no confirmed or proven ia was observed. mortality of ia was 18%. no serious adverse events due to itraconazole were observed. conclusions: the use of high dose itraconazole prophylaxis in adult patients with acute leukemia and severe neutropenia was associated to low incidence and mortality of invasive mycoses.
Damping of long-wavelength collective excitations in quasi-onedimensional Fermi liquids
F. Capurro,M. Polini,M. P. Tosi
Physics , 2002, DOI: 10.1016/S0921-4526(02)01541-7
Abstract: The imaginary part of the exchange-correlation kernel in the longitudinal current-current response function of a quasi-onedimensional Fermi liquid is evaluated by an approximate decoupling in the equation of motion for the current density, which accounts for processes of excitation of two particle-hole pairs. The two-pair spectrum determines the intrinsic damping rate of long-wavelength collective density fluctuations, which is calculated and contrasted with a result previously obtained for a clean Luttinger liquid.
Genotipificación del virus papiloma humano en mujeres con adenocarcinoma cervical de la Región de La Araucanía-Chile
Melo A,Angélica; García M,Patricia; Capurro V,Italo; Guzmán G,Pablo; Brebi M,Priscila; Ili G,Carmen; López M,Jaime; Roa S,Juan C;
Revista chilena de infectología , 2010, DOI: 10.4067/S0716-10182010000500001
Abstract: human papillomavirus (hpv) is the main cause of cervical cancer. thus, hpv detection and typing becomes important in order to know the frequency of genotypes present in the region. in this paper we studied 44 biopsies of cervical adenocarcinoma. for hpv detection nested polymerase chain reaction (pcr) was used to amplify the l1 gene. for viral typing restriction enzymes (rsa i, dde i, pst i) and dna sequencing were used. viral dna was detected by nested l1 pcr in 100% of biopsies; 38/44 cases could be typed: 81.6% hpv16; 13.2% hpv 18; 2.6% vph 33 and 2.6% hpv 18/33. conclusions: the technique was successful in identifying the virus type in 86% of biopsies. there was a strong association acc-hpv, especially with the viral type 16, detected in 81.6% of established cases.
Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino
LóPEZ M,JAIME; ILI G,CARMEN GLORIA; BREBI M,PRISCILLA; GARCíA M,PATRICIA; CAPURRO V,ITALO; GUZMáN G,PABLO; SUáREZ P,EUGENIO; OJEDA F,JOSé MANUEL; ROA S,JUAN CARLOS;
Revista médica de Chile , 2010, DOI: 10.4067/S0034-98872010001200001
Abstract: the relationship between human papillomavirus (hpv) and uterine cervical cancer (ucc) is widely known and accepted. aim: to determine the frequency of genotypes of hpv in cervical preneoplastic lesions in a high risk area of ucc. material and methods: using a combination of pcr and reverse line blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (lsil) or high-grade squamous intraepithelial lesion (hsil) were genotyped. results: hpv was detected in 61.2% of lsil and 78.1% of hsil. the main genotypes found were hpv 16, 18, 31, 45, 56 y 58. hpv 16 was the most common in both lsil (18.1%) and hsil (36.9%). hpv 16 or 18 were present in 25.1% and 47.1% of the lsil and hsil respectively. in both lsil and hsil, the predominant viral genotypes were those types classified as with a high oncogenic risk. conclusions: hpv genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. hpv 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of lsil and hsil, respectively.
On a Generalized Model in Accelerated Life Testing  [PDF]
Eduardo L. Cruz, Adolfo M. De Guzman
Open Journal of Statistics (OJS) , 2012, DOI: 10.4236/ojs.2012.22021
Abstract: The main objective of accelerated life tests in this setting is the recovery of the distribution of the random variable representing lifetime which is difficult to observe at a certain level of a given stress factor. A general model for accelerated life test is proposed that utilizes the inverse problem approach, that is, the variable is observe at different level/s and the transfer function is used to recover the elusive random variable (life time). The problem then is reduced to finding the transfer function. We derive some properties of the proposed general model. The Lognormal distribution and the Arrhenius model for lifetime are used as examples. Its relationship with the Cox proportional hazards model is also discussed.
Electrical parameters and water permeability properties of monolayers formed by T84 cells cultured on permeable supports
Ozu, M.;Toriano, R.;Capurro, C.;Parisi, M.;
Brazilian Journal of Medical and Biological Research , 2005, DOI: 10.1590/S0100-879X2005000100020
Abstract: t84 is an established cell line expressing an enterocyte phenotype whose permeability properties have been widely explored. osmotic permeability (posm), hydraulic permeability (phydr) and transport-associated net water fluxes (jw-transp), as well as short-circuit current (isc), transepithelial resistance (rt), and potential difference (dvt) were measured in t84 monolayers with the following results: posm 1.3 ± 0.1 cm.s-1 x 10-3; phydr 0.27 ± 0.02 cm.s-1; rt 2426 ± 109 w.cm2, and dvt 1.31 ± 0.38 mv. the effect of 50 μm 5,6-dichloro-1-ethyl-1,3-dihydro-2h-benzimidazol-2-one (dcebio), a "net cl- secretory agent", on t84 cells was also studied. we confirm the reported important increase in isc induced by dcebio which was associated here with a modest secretory djw-transp. the present results were compared with those reported using the same experimental approach applied to established cell lines originating from intestinal and renal epithelial cells (caco-2, llc-pk1 and rccd-1). no clear association between phydr and rt could be demonstrated and high phydr values were observed in an electrically tight epithelium, supporting the view that a "water leaky" barrier is not necessarily an "electrically leaky" one. furthermore, the modest secretory djw-transp was not consistent with previous results obtained with rccd-1 cells stimulated with vasopressin (absorptive fluxes) or with t84 cells secreting water under the action of escherichia coli heat stable enterotoxin. we conclude that, while the presence of aquaporins is necessary to dissipate an external osmotic gradient, coupling between water and ion transport cannot be explained by a simple and common underlying mechanism.
Electrical parameters and water permeability properties of monolayers formed by T84 cells cultured on permeable supports
Ozu M.,Toriano R.,Capurro C.,Parisi M.
Brazilian Journal of Medical and Biological Research , 2005,
Abstract: T84 is an established cell line expressing an enterocyte phenotype whose permeability properties have been widely explored. Osmotic permeability (P OSM), hydraulic permeability (P HYDR) and transport-associated net water fluxes (J W-transp), as well as short-circuit current (I SC), transepithelial resistance (R T), and potential difference (deltaV T) were measured in T84 monolayers with the following results: P OSM 1.3 ± 0.1 cm.s-1 x 10-3; P HYDR 0.27 ± 0.02 cm.s-1; R T 2426 ± 109 omega.cm2, and deltaV T 1.31 ± 0.38 mV. The effect of 50 μM 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one (DCEBIO), a "net Cl- secretory agent", on T84 cells was also studied. We confirm the reported important increase in I SC induced by DCEBIO which was associated here with a modest secretory deltaJ W-transp. The present results were compared with those reported using the same experimental approach applied to established cell lines originating from intestinal and renal epithelial cells (Caco-2, LLC-PK1 and RCCD-1). No clear association between P HYDR and R T could be demonstrated and high P HYDR values were observed in an electrically tight epithelium, supporting the view that a "water leaky" barrier is not necessarily an "electrically leaky" one. Furthermore, the modest secretory deltaJ W-transp was not consistent with previous results obtained with RCCD-1 cells stimulated with vasopressin (absorptive fluxes) or with T84 cells secreting water under the action of Escherichia coli heat stable enterotoxin. We conclude that, while the presence of aquaporins is necessary to dissipate an external osmotic gradient, coupling between water and ion transport cannot be explained by a simple and common underlying mechanism.
Damped Harmonic Oscillator with Arduino  [PDF]
L. H. M. de Castro, B. L. Lago, Felipe Mondaini
Journal of Applied Mathematics and Physics (JAMP) , 2015, DOI: 10.4236/jamp.2015.36075
Abstract: The benefits of using experiments in physics classes are widely discussed in the literature, but sometimes experimental setups are not available. In this paper we present different ways of using experiments in physics classes based on the Arduino board, since it involves low cost materials and can be built by the own students in several cases. In this work we addressed the well known damped harmonic oscillator and performed the data acquisition through the Arduino board, a LDR (Light Dependent Resistor), a infrared photodiode sensor and a computer. The setup of the proposed experiment and the technical details related to assembly are discussed in a clear way in order to be reproduced by anyone interested in the subject. We found a significant difference in the results obtained through the LDR and the photodiode. The later has given better results and has reproduced a regular decay in the amplitude of the oscillator even when the experiment was performed in a highly illuminated room. The Arduino board, alongside the referred peripherals, has shown great potential for building low cost experimental setups to be used in physics classes, both for expositive and hands on approaches.
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