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The pot experiments were conducted in the Department of Life Science and Bioinformatics of Assam University, Silchar. The aim of the present study was to investigate the effect of dual inoculation of Arbuscular mycorrhiza (AM) (Glomus fasciculatum) and Rhizobium on the chlorophyll, nitrogen and phosphorus contents of pigeon pea (Cajanus cajan L.). The results revealed an overall increase in chlorophyll, nitrogen and phosphorus contents in the inoculated plants than uninoculated ones. Maximum chlorophyll contents were recorded in the plants dually inoculated with Glomus fasciculatum and Rhizobium. The combined application of Glomus fasciculatum and Rhizobium also remarkably increased the nitrogen and phosphorus contents of pigeon pea. The dual inoculation with microsymbionts revealed synergistic effect. The results suggest that dual inoculation of Glomus fasciculatum and Rhizobium have the potential to enhance the chlorophyll, nitrogen and phosphorus contents of pigeon pea.
The extraction of zirconium (IV) from aqueous HCl solution by binary
mixture TOA and Cyanex 923 using kerosene as diluent was studied. The effect of
parameters like aqueous phase acid concentration, extractant concentration,
chloride ion concentration, diverse ion effect, metal loading, nature of
diluents and temperature on the extraction of Zr (IV) was investigated. The
percentage of extraction of Zr(IV) became 100% with 0.1 M TOA and 0.01 M Cyanex
923 from 7 M HCl and also from 4 M HCl with 0.1 M TOA and 0.02 M Cyanex 923. Benzene
was found to be effective diluent for the extraction of Zr(IV) with binary
mixture of TOA and Cyanex 923. The positive enthalpy change and positive
entropy change in the binary extraction system show endothermic process with increase
in randomness. Stripping of Zr (IV) from the loaded organic phase containing
mixture of TOA (0.1 M) and Cyanex 923 (0.01 M) indicated that HNO3 and Na2CO3 are the best stripping agents.