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Search Results: 1 - 10 of 544216 matches for " M. N. Brahmbhatt "
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Toxoplasmosis: An Important Protozoan Zoonosis
Sonar S. S. and Brahmbhatt M.N.
Veterinary World , 2010,
Abstract: Toxoplasmosis is an important infection caused by single celled parasite Toxoplasma gondii which is one of the world's most common parasites. Toxoplasmosis is considered to be the third leading cause of death attributed to food-borne illness in the United States. Most people affected never develop signs and symptoms. But for infants born to infected mothers and for people with compromised immune systems, toxoplasmosis can cause extremely serious complications. Toxoplasmosis was first described in 1908 from a small rodent. The parasite infects almost all worm blooded animals and serological evidence indicates that it is one of the most common of humans’ infections throughout the world. The disease is transmitted mainly by ingestion of infective stage of the parasite, organ transplant as well as blood transfusion in addition to the transplacental transmission which is very common. Toxoplasmosis can be presented in various forms of clinical manifestations depending on the immune status of the patient causing life threatening disease in AIDS patient. Pregnant women, cat owners, veterinarians, abattoir workers, children, cooks, butchers are considered as high risk group. Timely treatment of man and animals with proper antibiotic, hygienic measures, proper disinfection, mass education and vaccination are the measures to curtail the disease. [Veterinary World 2010; 3(9.000): 436-439]
Study on occurrence and antibiogram pattern of Escherichia coli from raw milk samples in Anand, Gujarat, India
H. C. Thaker,M. N. Brahmbhatt,J B Nayak
Veterinary World , 2012, DOI: 10.5455/vetworld.2012.556-559
Abstract: Aim: The study was carried out with aim to isolate Escherichia coli from raw milk samples and determine antibiogram pattern of E. coli isolates. Materials and Methods: During 6 months duration of study a total of 100 raw milk samples were collected from different places in and around Anand city such as individual household, cattle farms, milk collection centres of Co-operative milk dairies and milk vendors. All raw milk samples were enriched in peptone water and inoculated on selective media and various biochemical tests were performed for confirmation of isolates. Antibiogram pattern of E.coli to antimicrobial agents was evaluated by disk diffusion method. E. coli isolates were sent to National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, Himachal Pradesh for serotyping. Results: The result of present study revealed that out of 100 samples, 38 samples were found contaminated with E.coli. Antibiogram pattern revealed high resistance against ampicillin (100 %), whereas moderate resistance was observed for streptomycin (57.89 %), oxytetracycline (47.37 %) and amoxy-clav (42.11 %). Also lesser percentage of resistance was observed for co-trimoxazole (13.16 %) and chloramphenicol (5.26 %). Serotypes detected were O24 (7 isolates), followed by O36 (1 isolate), O89 (1 isolate), O91 (1 isolate) and O153(1 isolate). Conclusions: Results suggested a possibility of potential public health threat of E.coli originating from raw milk sources. [Vet World 2012; 5(9.000): 556-559]
Isolation and identification of Staphylococcus aureus from milk and milk products and their drug resistance patterns in Anand, Gujarat
H. C. Thaker,M. N. Brahmbhatt,J. B. Nayak
Veterinary World , 2013, DOI: 10.5455/vetworld.2013.10-13
Abstract: Aim: The study was carried out with aim to isolate Staphylococcus aureus from milk and milk products (pedha and curd) and determine antibiogram pattern of S. aureus isolates. Materials and Methods: During 9 months duration of study a total of 160 milk and milk product samples (pedha and curd) were collected from different places in and around Anand city such as milk collection centre of Co-operative milk dairies, cattle farms, individual household, milk vendors and sweet shops. The samples were collected under aseptic precautions and were enriched in Peptone Water (PW) followed by direct plating on selective media viz. Baird-Parker Agar. The presumptive S. aureus isolates were identified by biochemical tests. Antibiogram pattern of S. aureus to antimicrobial agents were evaluated by disk diffusion method. Results: Analysis of result revealed that out of total 160 samples of milk (100) and milk products i.e. curd (30) and pedha (30) resulted in the isolation of 10 isolates (6.25 %) of S. aureus. In the present study S. aureus isolates were found variably resistant to the antibiotics tested. The S. aureus isolates showed highest sensitivity towards cephalothin (100.00 %), co-trimoxazole (100.00 %), cephalexin (100.00 %) and methicillin (100.00 %) followed by gentamicin (90.00 %), ciprofloxacin (80.00 %), oxacillin (70.00 %), streptomycin (60.00 %) and ampicillin (60.00 %). The pattern clearly indicated that the overall high percent of S. aureus isolates were resistant to Penicillin-G (100.00 %) followed by ampicillin (40.00 %), oxytetracycline and oxacillin (20.00 %) and streptomycin and gentamicin (10.00 %) Conclusions: Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of milk and milk products with pathogenic bacteria is mainly due to unhygienic processing, handling and unhygienic environment. [Vet World 2013; 6(1.000): 10-13]
Micronuclei and chromosomal aberrations in healthy tobacco chewers and controls: A study from Gujarat, India
Patel Beena P.,Trivedi Pina J.,Brahmbhatt Manisha M.,Shukla Shilin N.
Archive of Oncology , 2009, DOI: 10.2298/aoo0902007p
Abstract: Background: Tobacco chewing is attributed to oral cancer. Prediction of cancer development by genotoxicity analysis is a major challenge to identify tobacco users at greater risk. Therefore, present study aimed to analyze tobacco related genotoxic effects in chewers monitoring micronuclei (MN) and chromosome aberrations (CA). The biomarkers were compared with non chewer to (i) predict risk for genotoxicity, (ii) estimate synergistic effect of tobacco exposure with level of biomarkers, and (iii) identify best cellular site of measurements for genotoxicity assessment. Methods: Healthy tobacco chewers (n=47); and controls (n=48) were enrolled in the study. The peripheral blood lymphocyte and exfoliated buccal mucosa cells were studied for CA and micro nucleated cell count (MNC) respectively. An arbitrary unit was obtained for Lifetime Tobacco Exposure (LTE) using frequency/day multiplied by duration of years of tobacco use. Data were analyzed using SPSS statistical software. Results: MNC was significantly higher (p=0.001) in chewers than controls. CA was higher in chewers than controls. MNC can differentiate higher tobacco exposure in chewers than CA. Controls having MNC above cutoff level have greater risk of genotoxic exposition (95% C.I.; 1.462-23.26, p=0.012). Conclusion: The present study concludes that MNC is a better surrogate biomarker to predict genotoxicity than CA for tobacco exposure and DNA damage index in tobacco chewers.
Arbovirus Detection in Insect Vectors by Rapid, High-Throughput Pyrosequencing
Kimberly A. Bishop-Lilly ,Michael J. Turell,Kristin M. Willner,Amy Butani,Nichole M. E. Nolan,Shannon M. Lentz,Arya Akmal,Al Mateczun,Trupti N. Brahmbhatt,Shanmuga Sozhamannan,Chris A. Whitehouse,Timothy D. Read
PLOS Neglected Tropical Diseases , 2010, DOI: 10.1371/journal.pntd.0000878
Abstract: Background Despite the global threat caused by arthropod-borne viruses, there is not an efficient method for screening vector populations to detect novel viral sequences. Current viral detection and surveillance methods based on culture can be costly and time consuming and are predicated on prior knowledge of the etiologic agent, as they rely on specific oligonucleotide primers or antibodies. Therefore, these techniques may be unsuitable for situations when the causative agent of an outbreak is unknown. Methodology/Principal Findings In this study we explored the use of high-throughput pyrosequencing for surveillance of arthropod-borne RNA viruses. Dengue virus, a member of the positive strand RNA Flavivirus family that is transmitted by several members of the Aedes genus of mosquitoes, was used as a model. Aedes aegypti mosquitoes experimentally infected with dengue virus type 1 (DENV-1) were pooled with noninfected mosquitoes to simulate samples derived from ongoing arbovirus surveillance programs. Using random-primed methods, total RNA was reverse-transcribed and resulting cDNA subjected to 454 pyrosequencing. Conclusions/Significance In two types of samples, one with 5 adult mosquitoes infected with DENV-1- and the other with 1 DENV-1 infected mosquito and 4 noninfected mosquitoes, we identified DENV-1 DNA sequences. DENV-1 sequences were not detected in an uninfected control pool of 5 adult mosquitoes. We calculated the proportion of the Ae. aegypti metagenome contributed by each infecting Dengue virus genome (pIP), which ranged from 2.75×10?8 to 1.08×10?7. DENV-1 RNA was sufficiently concentrated in the mosquito that its detection was feasible using current high-throughput sequencing instrumentation. We also identified some of the components of the mosquito microflora on the basis of the sequence of expressed RNA. This included members of the bacterial genera Pirellula and Asaia, various fungi, and a potentially uncharacterized mycovirus.
Thyroid Ultrasonography Consistently Identifies Goiter in Adults Over the Age of 30 Years Despite a Diminished Response with Aging of the Thyroid Gland to the Effects of Goitrogenesis
Sheela R. Brahmbhatt,Rajesh M. Brahmbhatt,Creswell J. Eastman,Steven C. Boyages
The Scientific World Journal , 2001, DOI: 10.1100/tsw.2001.55
Abstract:
Peculiar Characteristics of Fragmentation of Glaciers: A Case Study of Western Himalaya, India  [PDF]
Rupal M. Brahmbhatt, B. P. Rathore, Sandhyarani Pattnaik, Purnesh Jani, I. Bahuguna, R. D. Shah, A. S. Rajawat
International Journal of Geosciences (IJG) , 2015, DOI: 10.4236/ijg.2015.64035
Abstract: The areal extent of many Himalayan glaciers is decreasing where number of glaciers is increasing. This increasing number is subject to the fragmentation of limbs of main trunk glacier. However, disintegration of limbs is not observed in all the glacier system. This paper emphases the scenario of the fragmentation occurred in glaciers. Two glaciers from the two different basins have been taken into the consideration for this study. The peculiar characteristic of these glaciers is that, the tributary glaciers are showing less retreat in compare to main trunk glaciers. Due to this reason the rate of shift in snout is higher in main trunk glaciers than tributary glaciers. However, in recent data the tributary glaciers have experienced little higher loss in glacial ice than main glacier. But the shift of snout in main trunk glacier is no doubt surprising since past. Therefore, various parameters have been examined to identify the cause of such a behavior of glaciers. The common factors observed in both the glaciers are accumulation area ratio which is higher, snow line altitude which is lower in tributary glaciers for the period of 2005-2013. Another factor is the orientation as tributary glaciers are towards NE direction. In addition, slope of Dharlang glacier (main limb) is 4? where it is 18? in tributary glacier.
Assessment of post-operative pain in children: who knows best?
Anjalee Brahmbhatt,Tope Adeloye,Ari Ercole,Steven M. Bishop
Pediatric Reports , 2012, DOI: 10.4081/pr.2012.e10
Abstract: Pain assessment in children can be extremely challenging. Most professional bodies recommend that parents or carers should be involved with their child’s pain assessment; but the evidence that parents can accurately report pain on behalf of their children is mixed. Our objective was to examine whether there were differences in post-operative pain score ratings between the child, nurse and parent or carer after surgery. Cognitively intact children aged four upwards, undergoing all surgical procedures, whose parents were present in the post-anaesthetic recovery unit (PACU), were studied. Thirty-three children were included in the study. The numerical rating scale was used to rate the child’s pain by the child, nurse and parent on arrival to the PACU and prior to discharge. We found strong correlations between children’s, nurses’ and parent’s pain scores on admission and discharge from PACU. The intraclass correlation of pain scores reported by children, nurses and parents was 0.94 (95% confidence intervals 0.91-0.96, P<0.0001). In cognitively intact children, it is adequate to manage pain based upon the assessment of children’s and nurses’ pain scores alone. The numerical rating scale appeared to be suitable for younger children. Whilst there are benefits of parents being present in recovery, it is not essential for optimizing the assessment of pain.
A new recurring chromosome 13 abnormality in two older patients with de novo acute myeloid leukemia: An Indian experience
Trivedi P,Patel P,Brahmbhatt M,Patel B
Indian Journal of Human Genetics , 2009,
Abstract: We report here two cases of trisomy 13 in acute myeloid leukemia M1 subtype. short-term unstimulated bone marrow and peripheral blood lymphocyte culture showed 47, XY, +13 in all metaphase plates and trisomy 13 was confirmed with whole chromosome paint probes. Trisomy 13 in AML-M1 is a rare numerical abnormality. This is the first Indian report of sole trisomy 13 in AML-M1. Here, we present two cases of elder male patients, which may constitute a distinct subtype.
A case of acute myeloid leukemia-M2 with trisomy 4 in addition to t(8;21)
Trivedi P,Patel P,Brahmbhatt M,Patel B
Indian Journal of Human Genetics , 2008,
Abstract: t(8;21)(q22;q22) is the most frequently observed karyotypic abnormality associated with acute myeloid leukemia (AML), specifically in FAB-M2. Short-term unstimulated bone marrow (BM) and peripheral blood lymphocyte culture showed 47,XX, +4,t(8;21) in all metaphase plates; and interphase and metaphase results of AML-ETO fusion was positive and trisomy of 4 was confirmed with WCP probes. Trisomy 4 in AML with t(8;21) is a rare numerical abnormality. Here we present such case of patient which may constitute a distinctive subtype.
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