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Search Results: 1 - 10 of 401295 matches for " M. Mohebali "
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"Alterations of Serum Zinc, Copper and Iron Concentrations in Patients with Acute and Chronic Cutaneous Leishmaniasis"
M Faryadi,M Mohebali
Iranian Journal of Public Health , 2003,
Abstract: The aim of this study was to measure the alterations in serum zinc (Zn) copper (Cu) and iron (Fe) concentrations in patients with acute and chronic cutaneous leishmaniasis. Serum zinc and copper were measured by flameless atomic absorption spectrophotometer and serum iron concentration was measured by the Ferrozine method with commercial kits and assay was carried out by using an automatic analyzer. A total of 54 individuals were enrolled in this study: 18 patients with acute cutaneous leishmaniasis, 18 with chronic one and 18 healthy people who were not infected by Leishmania parasites. Serum Cu concentration was found to be significantly higher in the patients with acute (p<0.05) and chronic (p<0.05) cutaneous leishmaniasis than those of control group. However, Zn and Fe levels were lower in patients with acute (p<0.001) and chronic (p<0.001) cutaneous leishmaniasis than in the control group. There were no statistically significant differences in serum zinc, copper and iron levels in patients with acute and chronic cutaneous leishmaniasis. Our results showed that serum essential trace elements Zn, Cu and Fe concentrations have been changed in these patients. The changes may be a part of defense strategies of organism and are induced by the hormonelike substances.
Meriones Persicus as a New Probable Reservoir for Visceral Leishmaniosis
SSR Shojaei,M Mohebali
Iranian Journal of Public Health , 2005,
Abstract: Visceral leishmaniosis as a zoonotic disease is one of the important infectious disease due to severe side effects and mortality in children and because of at least two endemic foci in Iran (Firooz Abad and Djahrom in Fars State, Meshkin Shar and Moghan in Ardabil State). Despite all preventive procedures, the incidence of the disease has commonly increased which indicates the possible existence of other vertebrate’s reservoirs in addition to carnivoirs (main reservoirs of the Mediterranean visceral Leishmaniasis); therefore, rodents were examined during this research (1995-1997) in the endemic region of Meshkin Shahr. Comprehensive parasitological studies were conducted. Serological tests (Direct Agglutination Test) and cultivation protozoa were done in 120 sample rodents, which were captured alive. The prepared blood smears were undergone microscopic studies. Rodents that showed positive titers in DAT were also undergone necropsy .The prepared impression smears from Reticulo endothelial tissues (Liver and Spleen) were cultivated in NNN media. The observed livers and spleens of many rodents showed very low titers in many cases but showed significant pathological signs (Hepatomegally and splenomegally) even compared to higher titer rodents. The research procedure was changed by just considering the pathological signs of livers and spleens of sample rodents and ignoring the results of DAT .All the suspicious organs were cultivated in NNN. The captured rodents were Meriones persicus (116), Mus musculus (3) Cricetulus migratorius (1) and for the first time in Iran Leishmania donovani was isolated from M.persicus. In isoenzyme studies, zymodeme of isolated L.donvani , LON 50, was identified. Among 116 M.persicus 10.3% showed positive parasitological results and 52.6% had sero titers between 1:10 to 1:200 in DAT.
In Vivo Efficacy of Gum Obtained Pistacia Atlantica in Experimental Treatment of Cutaneous Leishmaniasis
M Taran,M Mohebali,J Esmaeli
Iranian Journal of Public Health , 2010,
Abstract: "nBackground: Recent circumstantial evidences are suggesting that an increasing number of Iranian patients with cu-taneous leishmaniasis are unresponsive to meglumine antimoniate (Glucantime ). Pistacia atlantica is native plant in Iran (central, western, and eastern regions). Gum obtained Pistacia atlantica has been reported to possess consid-erable in vitro antimicrobial activity. In this study, we aimed to investigate antileishmanial activity of P. atlantica."nMethods: Male BALB/c mice were inoculated subcutaneously 2×106 L. major Promastigotes (MHROM/IR/75/ER) at the base of tail in 2007. Mice were randomly divided into 3 groups. in group 1 Glucantime was administered to the BALB/c mice in regimen of 60 mg per kg of body weight for 28 days by intraperitoneal injections per day, in group 2 the gum of P. atlantica var. Kurdica were tested by rubbing of local lesions for 28 days, group 3 infected but non-treated. Comparisons of treated groups and untreated group were done by two-way analysis of variance (ANOVA)."n Results: Topically rubbing administration of gum obtained P. atlantica var. kurdica daily for 28 days like Glucan-time decreased skin lesion size in the BALB/c mice infected with L.major compared with that in the control (P< 0.01). Treatment BALB/c mice with gum obtained P. atlantica var. kurdica and Glucantime causes decrease num-ber of parasitologicaly positive mice (P< 0.05)."nConclusion: Our results show that gum obtained P. atlantica var. kurdica can be used for controlling cutaneous leishmaniasis caused by L.major and inhibiting development of cutaneous leishmaniasis lesions
"Rapid Detection of Pneumocystis Carini in Spiratory Specimens of Rats by Calcofluor White Staining"
M Mohebali,M Mirbakhsh,H Keshavarz
Iranian Journal of Public Health , 2002,
Abstract: The present study was carried out for evaluation of calcoflour white staining (CWS) as a rapid method for detection of Pneumocystis carinii in respiratory specimens of rats as an animal model for human infection. A total of 35 Spraque – Dawley rats were divided into two groups. Group1 (20 rats) received increasing doses of dexamethasone subcutaneously, and Group 2(15 rats)as control group that received no immunosuppressive drugs. After immunosuppressant, all of the rats were killed and necropsy was performed. Broncho-alveolar lavage (BAL) and impression smears from the lungs prepared and stained by CWS .The results were compared with a few standard staining methods which have already been used for P.carinii. The calcofluor white staining was found to have more validity (sensitivity and specificity) than other staining methods such as Geimsa , Modified Geimsa and Toluidine blue O ( TBO ) .The study showed the CWS to be more valid , faster and easier to perform for detecting of P. carinii rganism.
Prevalence of Trichomonas vaginalis Using Parasitological Methods in Tehran
M Rezaeian,M Vatanshenassan,S Rezaie,M Mohebali
Iranian Journal of Parasitology , 2009,
Abstract: "nBackground: Trichomonas vaginalis is a parasitic protozoan with a predilection for human urogenital tract and causative agent for vaginitis, cervicitis and urethritis in females. T. vaginalis is known as a cofactor in transmission of human immunodeficiency virus and may lead to adverse outcomes in pregnant women. The goal of this study was to determine the prevalence of T. vaginalis infection in females attending Mirzakuchak Khan Hospital, Tehran, Iran. "nMethods: During May 2008 to March 2009, 500 vaginal discharges samples were obtained from women attending sexual transmitted disease (STD) clinic of Mirzakuchak Khan Hospital in Tehran, Iran. The samples were examined by Dorsse culture medium and wet-mount meth-ods. The prevalence of T. vaginalis was determined using culture based method and wet-mount examinations. "nResults: Sixteen positive (3.2%) and 484 negative (96.8%) samples for T. vaginalis were de-tected by culture based methods. The wet mount examination revealed 13 positive (2.6%) and 487 negative (97.4%) samples. In the above population, prevalence of trichomoniasis was es-timated as 3.2% based on culturing method. "nConclusion: Due to adverse outcomes of vaginal trichomoniasis and its correlation with HIV transmission, there is a great need for public education regarding implementation of personal hygienic measures and prevention of inappropriate sexual contacts.
Neospora Caninum and Leishmania Infantum Co-Infection in Domestic Dogs (Canis Familiaris) in Meshkin-Shahr District, Northwestern Iran
M Sharifdini,M Mohebali,H Keshavarz,M Hosseininejad
Iranian Journal of Arthropod-Borne Diseases , 2011,
Abstract: Background: Mediterranean visceral leishmaniasis (MVL) is an infectious disease that affects both human and ani-mals. Domestic dogs (Canis familiaris) are principal reservoir hosts of MVL caused by Leishmania infantum. Dogs are definitive hosts for Neospora caninum and a risk factor for infecting intermediate hosts. The immunosuppression caused by visceral leishmaniasis (VL) can promote the occurrence of co-infections with other agents such as neosporo-sis. This study aimed to determine the frequency of co-infection of the both protozoan parasites in the en-demic areas of VL from Meshkin-Shahr District, north-west of Iran. Methods: Altogether, 171 serum samples were collected from domestic dogs of Meshkin- Shahr District by multistage cluster sampling from October 2008 to August 2009. The collected serum samples were tested for the detection of simultaneous infection of L. infantum and N. caninum using direct agglutination test (DAT) and indirect ELISA, respectively. Results: Of the 171 domestic dogs, 27 (15.8%) and 52 (30.4%) were showed antibodies against L. infantum and N. caninum, respectively. Simultaneous infections of N. caninum and L. infantum was found in 16 (9.4%) of the dogs. In VL-positive and VL-negative dogs, N. caninum infection was found in 59.3% and 25.0%, respectively. A statisti-cally significant difference was found between VL-positive and VL-negative dogs with N. caninum infection (P= 0.001). Conclusion: These findings indicate that Meshkin-Shahr District in northwestern Iran is an active focus of canine visceral leishmaniasis (CVL). Neospora caninum and L. infantum co-infection is prevalent in the area and infection by L. infantum seems to enhance susceptibility to N. caninum infection in domestic dogs.
Nanosilver in the treatment of localized cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER): an in vitro and in vivo study
M Mohebali,M.M Rezayat,K Gilani,S Sarkar
DARU : Journal of Pharmaceutical Sciences , 2009,
Abstract: "n "n "n "n "nBackground and the purpose of the study: "n This study was designed to evaluate the effectiveness of different concentrations of Nanosilver solution against Iranian strain of Leishmania major (MRHO/IR/75/ER) both in vitro and in vivo "n "non BALB/c mice model for the first time. "nMethods: "n this is an interventional study which was conducted on the infected macrophages by L. major amastigotes in vitro. In order to confirm the in vitro results, various concentration of Nanosilver solution were administered topically on skin lesions caused by L.major "n "nin 78 inbred BALB/c mice as test or interventional and 52 mice as control groups once or twice daily for 14 days. Results and major conclusion : Results of this study showed that different concentration of Nanosilver reduced proliferation of L. major amastigotes compared with the control wells but the differences were not statistically significant. Also, different concentrations of Nanosilver did not decrease the lesion sizes and amastigote counts in the BALB/c mice significantly. Secondary infection was significantly decreased in Nanosilver- treated groups compared with control groups (p<0.01). In conclusion, Nanosilver seems to be effective for control of secondary infection of localized cutaneous leishmaniasis.
CRICETULUS MIGRATORIUS (GRAY HAMSTER), ANOTHER POSSIBLE ANIMAL RESERVOIR OF KALA-AZAR IN MESHKIN-SHAHR, IRAN
M. Mohebali,M. Nasiri Kanari,A. Kanani,H. Edrissian
Iranian Journal of Public Health , 1995,
Abstract: Altogether, 120 Rodents of 4 species were trapped alive in various parts of Meshkin-Shar district where visceral leishmaniasis is endemic. 13 of them were Cricetulus migratorius (gray hamste) that all were caught inside the living rooms. Nine of them showed Leishmania antibody in titers of ≥ 1:100 in direct agglutination test (DAT) and amastigotes were found in the spleen smears of 2 Cricetulus migratorius One of them showed Leishmania antibody in titer of 1:400 and another one in titer 1:800 in DA test. This is the first report on the natural infection of a rodent with Leishmania in its internal organs in the endemic foci of visceral leishmaniasis in Iran.
Molecular Characterization and Sequencing of a Gene Encoding Mannose Binding Protein in an Iranian Isolate of Acanthamoeba castellanii as a Major Agent of Acanthamoeba Keratitis
M Niyyati,S Rezaie,F Rahimi,M Mohebali
Iranian Journal of Public Health , 2008,
Abstract: Background: Acanthamoeba castellanii is the important cause of amoebic keratitis in Iran. The key molecule in pathogenesis of Acanthamoeba keratitis is Mannose Binding Protein (MBP) led to adhesion of amoeba to corneal epithelium. Subsequent to adhesion other cytopathic effects occur. The goal of this study was to identify the molecular characterization of a gene encoding MBP in an Iranian isolate of A.castellanii in order to pave the way for further investigations such as new therapeutic advances or immunization. Methods: A.castellanii was cultured on non nutrient agar. Extraction of DNA was performed by phenol-chloroform method. After designing a pair of primer for the gene encoding MBP, PCR analysis was performed. Finally, the PCR product has been sequenced and the result submitted to the gene data banks. Results: An MBP gene of 1081 nucleotides was sequenced. This fragment contained three introns and encodes a protein with 194 amino acids. Homology search by Blast program showed a significant homology with the MBP gene in gene data banks (96%). Besides, the identity of amino acids with the other MBPs in gene data banks was about 86%. Conclusion: We isolated and sequenced a gene fragment encoding MBP in an Iranian isolate of A.castellanii. Molecular characterization of this important gene is the first step in pursuing researches such as developing better therapeutic agents, immunization of population at risk or even developing a diagnostic tool by PCR techniques.
Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen
M Taran,M Mohebali,MH Modaresi,S Mamishi
Iranian Journal of Public Health , 2007,
Abstract: Background: Surveillance of the canine reservoir is highly important to help control of visceral leishmaniasis in human. It is therefore imperative to improve and develop new tools reliable, easy to use, and cheap for the diagnosis of canine leishmani asis. K39 sub recombinant antigen of Leishmania infantum was expressed in prokaryotic system and evaluated for sero-diagnosis of canine visceral leishmaniasis (CVL). Methods: The gene fragment encoding a single 39-amino acid subunit of the kinesin-related protein k39 (k39sub) was ampli fied from DNA of Iranian strain of L. infantum (MCAN/IR/96/LON49) and cloned into a pMAL-p2 expres sion vector in frame with maltose-binding protein (MBP) fusion. The antigenic properties of L. infantum recombinant K39 sub unit (39 amino acids) have been tested for the serological diagnosis of CVL by ELISA. K39sub ELISA for CVL was com pared with a standard direct agglutination test (DAT) on 55 clinically infected dogs and 71 healthy controls from en demic areas of Ardabil and East Azerbaijan provinces, north-west of Iran. Results: A sensitivity of 72.7% and specificity of 87.3% were found at a 1:320 cut off titer when DAT confirmed cases were compared with healthy control. A good concordance was found between k39sub ELISA and DAT (k= 81.0). Conclusion: Given the antigenic properties shown by the k39sub, we think this protein carry immunodominant epitopes and are valuable for the sero- diagnosis of L. infantum infection in dogs.
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