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Search Results: 1 - 10 of 400776 matches for " M. Maione "
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Ultrastructure of human mature oocytes after vitrification
M.A. Khalili,M. Maione,M.G. Palmerini,S. Bianchi
European Journal of Histochemistry , 2012, DOI: 10.4081/ejh.2012.e38
Abstract: Since the introduction of human assisted reproduction, oocyte cryopreservation has been regarded as an attractive option to capitalize the reproductive potential of surplus oocytes and preserve female fertility. However, for two decades the endeavor to store oocytes has been limited by the not yet optimized methodologies, with the consequence of poor clinical outcome or of uncertain reproducibility. Vitrification has been developed as the promising technology of cryopreservation even if slow freezing remains a suitable choice. Nevertheless, the insufficiency of clinical and correlated multidisciplinary data is still stirring controversy on the impact of this technique on oocyte integrity. Morphological studies may actually provide a great insight in this debate. Phase contrast microscopy and other light microscopy techniques, including cytochemistry, provided substantial morphofunctional data on cryopreserved oocyte, but are unable to unraveling fine structural changes. The ultrastructural damage is one of the most adverse events associated with cryopreservation, as an effect of cryo-protectant toxicity, ice crystal formation and osmotic stress. Surprisingly, transmission electron microscopy has attracted only limited attention in the field of cryopreservation. In this review, the subcellular structure of human mature oocytes following vitrification is discussed at the light of most relevant ultrastructural studies.
Three-year observations of halocarbons at the Nepal Climate Observatory at Pyramid (NCO-P, 5079 m a.s.l.) on the Himalayan range
M. Maione, U. Giostra, J. Arduini, F. Furlani, P. Bonasoni, P. Cristofanelli, P. Laj,E. Vuillermoz
Atmospheric Chemistry and Physics (ACP) & Discussions (ACPD) , 2011,
Abstract: A monitoring programme for halogenated climate-altering gases has been established in the frame of the SHARE EV-K2-CNR project at the Nepal Climate Laboratory – Pyramid in the Himalayan range at the altitude of 5079 m a.s.l. The site is very well located to provide important insights on changes in atmospheric composition in a region that is of great significance for emissions of both anthropogenic and biogenic halogenated compounds. Measurements are performed since March 2006, with grab samples collected on a weekly basis. The first three years of data have been analysed. After the identification of the atmospheric background values for fourteen halocarbons, the frequency of occurrence of pollution events have been compared with the same kind of analysis for data collected at other global background stations. The analysis showed the fully halogenated species, whose production and consumption are regulated under the Montreal Protocol, show a significant occurrence of "above the baseline" values, as a consequence of their current use in the developing countries surrounding the region, meanwhile the hydrogenated gases, more recently introduced into the market, show less frequent spikes. Atmospheric concentration trends have been calculated as well, and they showed a fast increase, ranging from 5.7 to 12.6%, of all the hydrogenated species, and a clear decrease of methyl chloroform ( 17.7%). The comparison with time series from other stations has also allowed to derive Meridional gradients, which are absent for long living well mixed species, while for the more reactive species, the gradient increases inversely with respect to their atmospheric lifetime. The effect of long range transport and of local events on the atmospheric composition at the station has been analysed as well, allowing the identification of relevant source regions the Northern half of the Indian sub-continent. Also, at finer spatial scales, a smaller, local contribution of forest fires from the Khumbu valley has been detected.
Re-evaluating the contribution and legacy of Hedley Bull
Souza,Emerson Maione de;
Brazilian Political Science Review (Online) , 2008,
Abstract: the article aims, in the first instance, to make a detailed analysis of the work of hedley bull, approaching the main themes and concepts developed by him. secondly, it aims to re-evaluate the potential of the author's contribution, given the new conditions of the post-cold war period. with this in mind, the article critically analyses the most recent interpretations of his work, which seek to highlight its critical and normative potential, as well as to dissociate it from the realist tradition in international relations. these two facts differentiate the new commentators from older ones and reaffirm the continuing relevance of hedley bull's work, the latter being the article's chief conclusion.
Ordem e Justi?a na sociedade internacional pós-11 de Setembro
Souza, Emerson Maione de;
Revista Brasileira de Política Internacional , 2009, DOI: 10.1590/S0034-73292009000100007
Abstract: the article intends to analyze how the english school approach has examined international politics after september 11. it begins by analyzing some developments on the matter of justice that has taken place within the english school, highligning the solidarist conception of international society. afterwards the article analyses how the english school can help us to understand some of the main challenges that unipolarity poses on international society. it emphasize the limits of an order based on imposition and drives attention to the question of legitimacy and strengthening of international institutions.
Saída Diplomática na Bolívia
Meridiano 47 : Boletim de Análise de Conjuntura em Rela??es Internacionais , 2006,
Abstract: a
From international to world society? English school theory and the social structure of globalization
Emerson Maione de Souza
Contexto Internacional , 2007, DOI: 10.1590/s0102-85292007000200006
An extended Kalman-filter for regional scale inverse emission estimation
D. Brunner, S. Henne, C. A. Keller, S. Reimann, M. K. Vollmer, S. O'Doherty,M. Maione
Atmospheric Chemistry and Physics (ACP) & Discussions (ACPD) , 2012,
Abstract: A Kalman-filter based inverse emission estimation method for long-lived trace gases is presented for use in conjunction with a Lagrangian particle dispersion model like FLEXPART. The sequential nature of the approach allows tracing slow seasonal and interannual changes rather than estimating a single period-mean emission field. Other important features include the estimation of a slowly varying concentration background at each measurement station, the possibility to constrain the solution to non-negative emissions, the quantification of uncertainties, the consideration of temporal correlations in the residuals, and the applicability to potentially large inversion problems. The method is first demonstrated for a set of synthetic observations created from a prescribed emission field with different levels of (correlated) noise, which closely mimics true observations. It is then applied to real observations of the three halocarbons HFC-125, HFC-152a and HCFC-141b at the remote research stations Jungfraujoch and Mace Head for the quantification of emissions in Western European countries from 2006 to 2010. Estimated HFC-125 emissions are mostly consistent with national totals reported to UNFCCC in the framework of the Kyoto Protocol and show a generally increasing trend over the considered period. Results for HFC-152a are much more variable with estimated emissions being both higher and lower than reported emissions in different countries. The highest emissions of the order of 700–800 Mg yr 1 are estimated for Italy, which so far does not report HFC-152a emissions. Emissions of HCFC-141b show a continuing strong decrease as expected due to its controls in developed countries under the Montreal Protocol. Emissions from France, however, were still rather large, in the range of 700–1000 Mg yr 1 in the years 2006 and 2007 but strongly declined thereafter.
Influence of biomass burning and anthropogenic emissions on ozone, carbon monoxide and black carbon at the Mt. Cimone GAW-WMO global station (Italy, 2165 m a.s.l.)
P. Cristofanelli, F. Fierli, A. Marinoni, F. Calzolari, R. Duchi, J. Burkhart, A. Stohl, M. Maione, J. Arduini,P. Bonasoni
Atmospheric Chemistry and Physics (ACP) & Discussions (ACPD) , 2013,
Abstract: This work investigates the variability of ozone (O3), carbon monoxide (CO) and equivalent black carbon (BC) at the Italian Climate Observatory "O. Vittori" (ICO-OV), part of the Mt. Cimone global GAW-WMO station (Italy). For this purpose, ICO-OV observations carried out in the period January 2007–June 2009, have been analyzed and correlated with the outputs of the FLEXPART Lagrangian dispersion model to specifically evaluate the influence of biomass burning (BB) and anthropogenic emissions younger than 20 days. During the investigation period, the average O3, CO and BC at ICO-OV were 54 ± 3 ppb, 122 ± 7 ppb and 213 ± 34 ng m 3 (mean ± expanded uncertainty with p < 95%), with clear seasonal cycles characterized by summer maxima and winter minima for O3 and BC and spring maximum and summer minimum for CO. According to FLEXPART outputs, BB impact is maximized during the warm months from July to September but appeared to have a significant contribution to the observed tracers only during specific transport events. We characterised in detail five "representative" events with respect to transport scales (i.e. global, regional and local), source regions and O3, CO and BC variations. For these events, very large variability of enhancement ratios O3/CO (from 0.22 to 0.71) and BC/CO (from 2.69 to 29.83 ng m 3 ppb 1) were observed. CO contributions related with anthropogenic emissions (COant) contributed to 17.4% of the mean CO value observed at ICO-OV, with the warm months appearing particularly affected by transport events of air-masses rich in anthropogenic pollution. The proportion of tracer variability that is described by FLEXPART COant peaked to 37% (in May–September) for CO, 19% (in May–September) for O3 and 32% (in January–April) for BC. During May–September, the analysis of the correlation among CO, O3 and BC as a function of the COant indicated that ICO-OV was influenced by air-masses rich in anthropogenic pollution transported from the regional to the global scale. On the other side, CO and O3 were negatively correlated during October–December, when FLEXPART does not show significant presence of recent anthropogenic emissions and only a few observations are characterized by enhanced BC. Such behaviour may be attributed to an ensemble of processes concurrent in enhancing O3 with low CO (upper troposphere/lower stratosphere intrusions) and to O3 titration by NO in polluted air-masses along with lower photochemical activity. An intermediate situation occurs in January–April when CO and O3 were almost uncorrelated and BC enhancements were associated to relatively old (10 days) anthropogenic emissions.
Value Co-Creation Practices in Smart City Ecosystem  [PDF]
Marco Pellicano, Mario Calabrese, Francesca Loia, Gennaro Maione
Journal of Service Science and Management (JSSM) , 2019, DOI: 10.4236/jssm.2019.121003
Abstract: Starting from the definition of smart cities, characterized by flexible information processes, innovation facilitation mechanisms, smart and sustainable solutions and platforms, the new urban scenario highlights the creation of value as the core purpose and the central process of exchange between actors. According to this consideration, the present work aims at re-reading smart cities in the light of value co-creation practices, trying to capture the factors that local administrators can leverage to pursue a more acute development of local communities. The work follows an exploratory approach by using, in particular, the single case model (holistic), with the purpose to analyse the value co-creation practices (Frow et al., 2014) in the context of smart city. Specifically, the city of Turin has been chosen, focusing on the initiative promoted along with the Torino smart city Foundation. Eight value co-creation practices are identified, in order to determine the set of attributes leading to the transformation process in smart cities. In addition, for each type of practice specific measures are identified. The work offers insights to improve the results pursued in different areas of administrative life. In fact, value co-creation practices highlight that an adequate combination of activities carried out by administrators, citizens-users, technology and other players in social life leads to improved performance in the urban sector. Furthermore, the work underlines the importance of value-added practices as tools to facilitate the involvement of several social actors, who are differently interested in contributing to the definition of processes of value generation. The innovative nature of the work arises from the authors’ choice to analyse the theoretical background on practices in terms of co-created value and to connect these practices to smart cities, enabling arriving at a conceptual result steeped in value for scholars and practitioners interested in both business management and technology engineering.
Peptide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent
Accardo A, Salsano G, Morisco A, Aurilio M, Parisi A, Maione F, Cicala C, Tesauro D, Aloj L, De Rosa G, Morelli G
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S29242
Abstract: ide-modified liposomes for selective targeting of bombesin receptors overexpressed by cancer cells: a potential theranostic agent Original Research (2748) Total Article Views Authors: Accardo A, Salsano G, Morisco A, Aurilio M, Parisi A, Maione F, Cicala C, Tesauro D, Aloj L, De Rosa G, Morelli G Published Date April 2012 Volume 2012:7 Pages 2007 - 2017 DOI: http://dx.doi.org/10.2147/IJN.S29242 Received: 16 December 2011 Accepted: 20 January 2012 Published: 17 April 2012 Antonella Accardo1,2*, Giuseppina Salsano3*, Anna Morisco4, Michela Aurilio4, Antonio Parisi5, Francesco Maione5, Carla Cicala5, Diego Tesauro1,2, Luigi Aloj4, Giuseppe De Rosa3, Giancarlo Morelli1,2 1CIRPeB, Department of Biological Sciences and IBB CNR, University of Naples “Federico II”, 2Invectors srl, 3Department of Pharmaceutical Chemistry, University of Naples “Federico II”, 4Department of Nuclear Medicine, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione “G. Pascale”, 5Department of Experimental Pharmacology, University of Naples “Federico II”, Napoli, Italy *These authors contributed equally to this work Objectives: Drug delivery systems consisting of liposomes displaying a cell surface receptor-targeting peptide are being developed to specifically deliver chemotherapeutic drugs to tumors overexpressing a target receptor. This study addresses novel liposome composition approaches to specifically target tissues overexpressing bombesin (BN) receptors. Methods: A new amphiphilic peptide derivative (MonY-BN) containing the BN(7–14) peptide, the DTPA (diethylenetriaminepentaacetate) chelating agent, a hydrophobic moiety with two C18 alkyl chains, and polyethylene glycol spacers, has been synthesized by solid-phase methods. Liposomes have been generated by co-aggregation of MonY-BN with 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). The structural and biological properties of these new target-selective drug-delivery systems have been characterized. Results: Liposomes with a DSPC/MonY-BN (97/3 molar ratio) composition showed a diameter of 145.5 ± 31.5 nm and a polydispersity index of 0.20 ± 0.05. High doxorubicin (Dox) loading was obtained with the remote pH gradient method using citrate as the inner buffer. Specific binding to PC-3 cells of DSPC/MonY-BN liposomes was obtained (2.7% ± 0.3%, at 37°C), compared with peptide-free DSPC liposomes (1.4% ± 0.2% at 37°C). Incubation of cells with DSPC/MonY-BN/Dox showed significantly lower cell survival compared with DSPC/Dox-treated cells, in the presence of 100 ng/mL and 300 ng/mL drug amounts, in cytotoxicity experiments. Intravenous treatment of PC-3 xenograft-bearing mice with DSPC/MonY-BN/Dox at 10 mg/kg Dox dose produced higher tumour growth inhibition (60%) compared with nonspecific DSPC/Dox liposomes (36%) relative to control animals. Conclusion: The structural and loading properties of DSPC/MonY-BN liposomes along with the observed in-vitro and in-vivo activity are encouraging for further development of t
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