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Search Results: 1 - 10 of 401297 matches for " M. Chiarini "
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Cobertura vegetal do estado de S?o Paulo: I - Levantamento por fotointerpreta??o das áreas cobertas com cerrado, cerrad?o e campo, em 1962
Borgonovi, Mário;Chiarini, Jorge Vicente;
Bragantia , 1965, DOI: 10.1590/S0006-87051965000100014
Abstract: a survey was achieved in 1962 based on aerial photographs covering the whole area of the state of s?o paulo and areas were separated, by photo-interpretation, according to the type of vegetation, as follows: the ?cerrado? which is a type of brazilian savanna, the ?cerrad?o?, a type of shrubbery, mostly tall and rather dense, and grasslands, a type of herbaceous vegetation. in the characterization of the latter were considered just those areas that had not been under cultivation as yet or with but a little interference by man, altering somewhat their texture. photo-interpretation was performed on a basis of characterization of texture by field sampling. this paper shows the situation with regard to the various types of plants covering the areas: ?cerrado? covered 724,900 ha, ?cerrad?o? 2,668,000 and grasslands 458,600 ha, these figures representing 2.9, 10.8 and 1.8% of the total area of the state.
Crescimento e diferencia??o "In vitro" de cepas de Trypanosoma cruzi, isoladas de animais silvestres
Mello,Dalva A.; Borges,Monamaris M.; Chiarini,Lúcia Helena;
Revista de Saúde Pública , 1980, DOI: 10.1590/S0034-89101980000400012
Abstract: in the course of this work, four strains of sylvatic trypanosoma cruzi were studied in regard to their growth and differentiation in lit medium. r52, r64, and r65 were isolated from individual didelphis albiventris (marsupialia) and m226 from calomys callosus (rodentia). the evolution of the numerical incresase and morphogenesis of the trypanosome populations in the culture was observed at regular intervals during a period of 312 hours. differential counts were made of amastigotes, epimastigotes, and trypomastigotes in neubauer chambers. results showed that the four strains had characteristic behavior patterns. m226 increased the most and was followed by r65, r52, and then r64.
Composición florística del Bosque Chaque o Serrano de la provincia de Córdoba, Argentina Floristic composition of the Chaco Serrano Woodland in Córdoba province, Argentina
Melisa A Giorgis,Ana M Cingolani,Franco Chiarini,Jorge Chiapella
Kurtziana , 2011,
Abstract: Si bien el Chaco Serrano constituye una de las principales unidades fitogeográficas de la provincia de Córdoba, son escasos y restringidos los relevamientos florísticos disponibles. Por ello, en este trabajo se analiza la composición florística del Chaco Serrano entre los 400 y 1700 m.s.m. A lo largo de los cuatro cordones monta osos que lo componen, realizamos 437 relevamientos completos de plantas vasculares, en cuadrados homogéneos de 20 × 20 m. Se registraron un total de 106 familias, 476 géneros y 896 especies de plantas vasculares. Las dos familias mejor representadas fueron Poaceae (157) y Asteraceae (143). Los géneros con mayor número de especies correspondieron a Baccharis, Eupatorium, Euphorbia, Gymnocalycium y Tillandsia. Las formas de vida con mayor número de especies fueron hierbas perennes siempre-verdes (221), graminoides (143) y arbustos (124); los árboles nativos registrados fueron 27. Del total de especies registradas, 102 (11%) fueron exóticas, 29 de ellas le osas. Se registraron aproximadamente la mitad de especies previamente citadas para la provincia de Córdoba. Además, se mencionan 6 citas nuevas para la provincia. Estos resultados ponen de manifiesto la importancia de la unidad fitogeográfica del Chaco Serrano para la conservación de la flora de la provincia de Córdoba. The Chaco Serrano constitutes one of the main phytogeographic units of Córdoba province; however, available floristic information is limited and restricted. In this contribution we characterize the floristic composition of the Chaco Serrano between 400 and 1700 m a.s.l. We performed 437 comprehensive floristic samples of vascular plants in homogeneous quadrates of 20 × 20 m. A total of 106 families, 476 genera and 896 vascular plants species were recorded. Poaceae (157) and Asteraceae (143) are the best represented families while Baccharis, Eupatorium, Euphorbia, Gymnocalycium and Tillandsia are the genera with the highest number of species. Perennial evergreen herbs (221), graminoids (143) and shrubs (124) are the life forms with the highest number of species. We recorded 27 native trees species and 102 (11% of the total recorded species) alien species, 29 of them were woody. Approximately half of the species previously reported for the whole Córdoba province were recorded in this phytogeographic unit. Additionally, we provide six new records for Córdoba. These findings highlight the importance of the Chaco Serrano phytogeographic unit for the conservation of Córdoba flora.
Comparison of different extenders for holding pheasant semen
M. Marzoni,A.G. Castillo,R. Chiarini,I. Romboli
Italian Journal of Animal Science , 2011, DOI: 10.4081/ijas.2003.s1.184
Abstract: For the last decades several studies for holding sperm have been done in various fowl species. Consequently different conditions were proposed, changing storage times, temperature, and adapting semen extenders for each situation. However outstanding development has been reached, each species and conditions demand special needs. Our previous studies were mainly conducted on the evaluation of the semen quality parameters in pheasants (Marzoni et al., 2000a,b; Marzoni et al., 2001; Chiarini et al., 2002; Marzoni et al., 2002), thus the present study focused on the use of three different semen extenders to preserve pheasant semen over a 24-hours period........
Analysis of regulatory T-cells and of their na ve and memory-like subsets in long-term treated aviremic HIV+ patients and untreated viremic patients
E Quiros-Roldan,F Serana,M Chiarini,D Gotti
Journal of the International AIDS Society , 2012, DOI: 10.7448/ias.15.6.18195
Abstract: Purpose of the study: Although HIV infection impacts the proportion and phenotype of regulatory T-cells (Tregs), discrepant results have been reported depending on the surface markers employed to characterize them and on the patient populations. In addition, the effects of a long-term combined antiretroviral therapy (cART) on Treg cells have not been thoroughly documented. Our study investigated the frequency and number of Tregs and their phenotype in two different groups of HIV-infected patients: one aviremic undergoing long-term cART and one viremic na ve to cART showing a similar CD4+ cell count. Methods: Thirty-six HIV+ patients with sustained suppression of plasma viremia (<37 copies/mL) on effective cART for more than 6 years and 22 HIV+patients na ve to cART and without clinical signs of opportunistic infections or tumors at the time of study (untreated group) were included in the study. Healthy donors (HD) were used as control. Flow cytometry on fresh whole blood was used to quantify total Tregs (defined as CD25+CD127low/-CD4+ cells) and the following Treg subsets: na ve (CD45RA+CCR7+) Tregs, central-memory like Tregs (CD45RA-CCR7+, TregCM), effector-memory like Tregs (CD45RA-CCR7 , TregEM) Statistical comparisons of the percentages and number of Tregs and Treg subpopulations were performed by ANOVA or Kruskal-Wallis test. Analysis of covariance was employed in order to adjust for the effect of the age. The Spearman's test was used to assess correlations. Summary of results: In viremic untreated and aviremic long-term cART-treated patients the percentage and number of the total Treg cells were not different from those of HD. However, the analysis of Treg phenotype showed a marked redistribution of the Treg subpopulations: in the untreated viremic patients, both the percentage and number of the TregCM subset decreased compared to HD and cART-treated patients, whereas only the percentage of na ve Tregs increased. In particular, the percentage of TregCM was inversely correlated with the viral load (r= 0.51; p=0.016). Conclusions: In our aviremic long-term cART-treated and viremic untreated patients, the total Treg cell population seems to be unaffected by HIV infection. However, our results showed that the analysis of the na ve and memory-like Treg subsets may provide a better understanding of the real contribution of Tregs in HIV disease and therapy.
Solanaceae (Continuación)
Franco Chiarini
Multequina , 2010,
The Emerging Role of the Phosphatidylinositol 3-Kinase/ Akt/Mammalian Target of Rapamycin Signaling Network in Cancer Stem Cell Biology
Alberto M. Martelli,Camilla Evangelisti,Francesca Chiarini,Cecilia Grimaldi,James A. McCubrey
Cancers , 2010, DOI: 10.3390/cancers2031576
Abstract: The cancer stem cell theory entails the existence of a hierarchically organized, rare population of cells which are responsible for tumor initiation, self-renewal/maintenance, and mutation accumulation. The cancer stem cell proposition could explain the high frequency of cancer relapse and resistance to currently available therapies. The phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway regulates a wide array of physiological cell functions which include differentiation, proliferation, survival, metabolism, autophagy, and motility. Dysregulated PI3K/Akt/mTOR signaling has been documented in many types of neoplasias. It is now emerging that this signaling network plays a key role in cancer stem cell biology. Interestingly, cancer stem cells displayed preferential sensitivity to pathway inhibition when compared to healthy stem cells. This observation provides the proof-of-principle that functional differences in signaling pathways between neoplastic stem cells and healthy stem cells could be identified. In this review, we present the evidence which links the signals emanating from the PI3K/Akt/mTOR cascade with the functions of cancer stem cells, both in solid and hematological tumors. We then highlight how targeting PI3K/Akt/mTOR signaling with small molecules could improve cancer patient outcome.
Long-term culture of cholangiocytes from liver fibro-granulomatous lesions
Luciana B Chiarini, Christina M Takiya, Radovan Borojevic, Alvaro NA Monteiro
BMC Gastroenterology , 2006, DOI: 10.1186/1471-230x-6-13
Abstract: We have isolated a cholangiocyte cell line from Schistosoma-induced liver granulomas using a combination of methods including selective adhesion and isopyknic centrifugation in Percoll.The cell line was characterized by morphological criteria in optical and transmission electron microscopy, ability to form well differentiated ductular structures in collagen gels and by a positive staining for cytokeratin 18 and cytokeratin 19. To our knowledge, this is the first murine cholangiocyte cell line isolated from schistosomal fibrosis reported in the literature.After 9 months and 16 passages this diploid cell line maintained differentiated characteristics and a high proliferative capacity. We believe the method described here may be a valuable tool to study bile duct changes during hepatic injury.Extensive bile duct proliferation is a key feature of the tissue reaction to clinical and experimental forms of liver injury and in many cases, this proliferation may affect liver function [1,2]. It has long been appreciated that bile duct epithelial cells can be isolated and cultured in vitro from human [3-7] and animal liver tissue [8-12]. Cultures of bile duct epithelia have been derived from normal, cholestatic, or carcinogen-treated livers [10,11]. Although experimental infection of mice by Schistosoma mansoni is a well studied model of liver fibrosis with bile duct hyperplasia [13](Fig. 1A–F), cholangiocytes have not yet been isolated from schistosomal livers and characterized in vitro. In experimental schistosomiasis, a spectrum of pathologic changes of the intrahepatic bile ducts can be observed, such as hyperplasic epithelial lining made up of hypertrophic cells or cells with nuclei disposed in variable height and periductular fibrosis [13]. The origin and nature of these bile duct cells remain unknown since there has been no systematic study of the cells implicated in bile duct hyperplasia during Schistosoma infection.Since certain chronic disorders of the biliary tract
Anatomical study of different fruit types in Argentine species of Solanum subgen. Leptostemonum (Solanaceae)
Barboza, Gloria,Chiarini, Franco
Anales del Jardín Botánico de Madrid , 2007,
Abstract: The fruits of 11 species of Solanum subgen. Leptostemonum were studied. Cross and/or longitudinal microtome sections, stained mostly with astra blue/basic fuchsin, were prepared for microscopic examination. The fruits, notably heterogeneous, were classified into three categories. Three different kinds of cells were found of the epidermis, immediately below which a hypodermis, consisting in any of five types of structures, was always found. The mesocarp presented two histologically differentiated zones, an external one (formed by normal or spongy parenchyma, depending on the species), and an internal one, commonly juicy, and with proliferations among the seeds. This morpho-anatomical information was used to distinguish between non-capsular dehiscent fruits and the berry traditionally described for Solanum. The relationship between structure and function, and the probable dispersal syndromes are also discussed. Se estudiaron los frutos de 11 especies de Solanum subgen. Leptostemonum. Para ello, se efectuaron cortes microtómicos longitudinales y/o transversales, te idos en su mayor parte con azul astral/fucsina, y fueron examinados al microscopio. Los frutos, notablemente heterogéneos, fueron clasificados en tres categorías. Tres diferentes tipos de células fueron encontrados en la epidermis, e inmediatamente por debajo se observó siempre una hipodermis, constituida por uno de cinco tipos de estructuras. El mesocarpio presentó dos zonas histológicamente diferenciadas: una externa (formada por parénquima normal o esponjoso, según la especie) y una interna, comúnmente jugosa y con proliferaciones entre las semillas. Esta información morfoanatómica fue usada para distinguir entre el fruto dehiscente no capsular y la baya tradicionalmente descrita para Solanum. Se discutieron además la relación entre estructura y función y los probables síndromes de dispersión.
A expans o da língua italiana na península e a sala de aula de italiano como língua estrangeira
Ana Maria Chiarini
Caligrama : Revista de Estudos Romanicos , 2005,
Abstract: Resumo: Este trabalho se prop e a oferecer um panorama do processo de italianiza o lingüística da península italiana, acentuando a riqueza e a complexidade da situa o dele resultante. Partindo da premissa de que a sala de aula de italiano como LE n o está, nem deve estar, imune à dinamica da língua no cenário onde é falada, o objetivo deste texto é sensibilizar o profissional do ensino ou o aprendiz para tal dinamica e evidenciar o fato de que apenas o conhecimento dessa realidade possibilitará a clareza para a defini o de conteúdos lingüísticos e culturais das aulas e para um posicionamento mais crítico e seguro diante das incertezas e ambigüidades de um campo em saudável movimento. Palavras-chave: Língua italiana; península italiana; expans o. Abstract: Il presente lavoro si propone di offrire un panorama del processo di italianizzazione linguistica della penisola italiana, sottolineando la ricchezza e la complessità della situazione che ne è risultata. Partendo dalla premessa che la lezione d’italiano come lingua straniera non è e non deve essere immune dalla dinamica della lingua nello scenario dove è parlata, l’obiettivo di questo testo è, dunque, quello di sensibilizzare l’insegnante o l’apprendente a tale dinamica e evidenziare il fatto che soltanto la conoscenza di questa realtà consentirà una maggior chiarezza nella definizione dei contenuti linguistici e culturali delle lezioni e dell’atteggimento più critico e sicuro nei confronti delle incertezze e ambiguità di questo campo in salutare movimento. Parole chiave: Lingua italiana; penisola italiana; espansione.Keywords: Italian; Italian Peninsula; expansion.
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