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Search Results: 1 - 10 of 715046 matches for " M. A. Chagas "
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Anormalidades de tangerineiras 'ponc?', no estado de Minas Gerais, provocadas por deficiência de boro
Quaggio, José A.;Rossetti, Victoria;Chagas, Cesar M.;
Bragantia , 1996, DOI: 10.1590/S0006-87051996000100020
Abstract: in 1993 abnormalities observed on poncan mandarin (citrus reticulata blanco), in the area of perd?es, state of minas gerais, were suspected to be related to "greening", a citrus disease, not yet detected in brazil. the affected plants presented very small and hard fruits, with thick and rough peel and drop prematurely. cutting these fruits, conspicuous formation of gum pockets was seen in the albedo and some also in the flavedo, similar to boron deficiency. the seeds were dark and aborted. the normal fruits of the same trees did not show these symptoms. leaf and fruit material were collected, as well as soil samples, at two depths. leaves taken from symptomatic branches, have 6 to 12 tng/kg of boron, too low in relation with normal ones of 30 to 40 mg/kg. leaf application of 1.5 mg/l of boric acid solution have promoted normal growth, 30 days after the application. results of electronic microscopy did not indicate the presence of greening bacteria from neither foliar midribs nor fruits columella. a brief description of greening symptoms is presented. it was concluded that the abnormalities are due to boron deficiency instead of citrus greening as previously reported.
Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S.;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000100015
Abstract: the otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ans). similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. we have studied rat otic ganglion in 10 animals through light microscopy. after routine histological methods, 5 μm sections were obtained and coloured by gomori`s trichrome, periodic acid-schiff (pas), alcian blue ph 2.5 and ph1.0, acetylation + pas, acetylation + deacetylation + pas, acid hydrolysis + pas, phenylhydrazine + pas and thiosemicarbazide + pas. the presence of neutral glycoproteins was demonstrated by pas reactivity. pas inhibition following alcian blue staining in ph 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. the extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. these findings suggests a mutual interaction and a complex role in ganglionic physiology
MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL
Cortez,C. M.; Costa,W. S.; Babinski,M. A.; Chagas,M. A.;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000300006
Abstract: the present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. the study was based on morphometric and stereological analysis of the perikarya. eight rats were treated intraperitoneally with cortisol (1mg/kg/day) during 36 days. treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. there was a significant difference (£p0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). the decrease of perikarya neuronal diameter was also demonstrated by stereological methods. morphometrical findings may suggest alterations in superior cervical ganglion neuronal activity in rats treated for long term with cortisol
MORFOLOGíA Y FRACCIóN DEL áREA DEL LUMEN GLANDULAR DE LA ZONA DE TRANSICIóN EN LA PRóSTATA HUMANA
Babinski,M. A.; Chagas,M. A.; Costa,W.S.; Pereira,M. J.;
Revista chilena de anatomía , 2002, DOI: 10.4067/S0716-98682002000300004
Abstract: although the male reproductive system diseases are not restricted to the prostate, this is one of the most interesting organs for the urologic medical practice. the benign prostatic hyperplasia (bph) represents a tissual increase of these histological elements causing profound alterations in the prostate organisation. therefore this study aims at evaluating the glandular lumen volumetric density (vv) the transition zone (median lobe) in human normal prostates compared with hyperplastic ones (bph). controls consisted of 10 samples of prostates transition region obtained during necropsy of young adults victims of accidents. samples of prostatic tissue of the transition zone were obtained from 10 patients with clinical symptoms of bladder outlet obstruction and histopathological diagnoses of the bph submitted to transvesicalprostatectomy. samples of the 5mm were stained on paraffin sections of prostate using hematoxilin-eosin. for the quantitative analysis the stereology method assisted by a software program was used to verify the vv. the glandular lumen volumetric density showed the significant statistically bph increase, which seems to be related to the bladder outlet obstruction and to the stasis of the luminal contents, causing intraluminal pressure in turn deformations in the glandular with probable consequences in the epithelium, well the to the secretory alterations.
MORFOLOGíA Y FRACCIóN DEL áREA DEL LUMEN GLANDULAR DE LA ZONA DE TRANSICIóN EN LA PRóSTATA HUMANA MORPHOLOGY AND AREAL FRACTION OF THE GLANDULAR LUMEN OF TRANSITION ZONE IN THE HUMAN PROSTATE
M. A. Babinski,M. A. Chagas,W.S. Costa,M. J. Pereira
Revista chilena de anatomía , 2002,
Abstract: Aunque las enfermedades del sistema reproductor masculino no se restringen a la próstata, éste es uno de los órganos más interesantes para la práctica médica urológica. La hiperplasia prostática benigna (HPB) representa un aumento tisular de estos elementos histológicos que causan alteraciones profundas en el organización de la próstata. Este estudio pretende evaluar la densidad volumétrica (VV) del lumen glandular de la zona de transición (lóbulo mediano) en próstatas humanas normales, comparadas con hiperplásicas (HPB). Muestras de tejidos prostáticos de la zona de transición fueron obtenidas de 10 pacientes con síntomas de obstrucción infravesical y diagnóstico histopatológico del HPB, sometidos a prostatectomía transvesical. Para control fueron utilizadas 10 muestras de la región de transición prostática, obtenidas durante la necroscopía de jóvenes adultos, víctimas de accidentes. Los bloques de parafina de muestras prostáticas fueron seccionados con 5μm de grosor y te idos con Hematoxilina y Eosina. Para el análisis cuantitativo, fue usado el método estereológico asistido por un programa de software para verificar la VV. La densidad volumétrica del lumen glandular, mostró un incremento estadísticamente significativo de la HPB, y esto parece estar relacionado con la obstrucción infravesical y el éstasis del contenido luminal, aumentando la presión intraluminal y, por tanto, causa deformaciones de las glándulas con probables consecuencias en el epitelio, así como alteraciones secretorias. Although the male reproductive system diseases are not restricted to the prostate, this is one of the most interesting organs for the urologic medical practice. The benign prostatic hyperplasia (BPH) represents a tissual increase of these histological elements causing profound alterations in the prostate organisation. Therefore this study aims at evaluating the glandular lumen volumetric density (VV) the transition zone (median lobe) in human normal prostates compared with hyperplastic ones (BPH). Controls consisted of 10 samples of prostates transition region obtained during necropsy of young adults victims of accidents. Samples of prostatic tissue of the transition zone were obtained from 10 patients with clinical symptoms of bladder outlet obstruction and histopathological diagnoses of the BPH submitted to transvesicalprostatectomy. Samples of the 5mm were stained on paraffin sections of prostate using hematoxilin-eosin. For the quantitative analysis the stereology method assisted by a software program was used to verify the VV. The glandular lumen volumetric den
MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL ANáLISIS MORFOMéTRICO Y ESTEREOLóGICO DEL GANGLIO CERVICAL SUPERIOR DE Rattus norvegicus SOMETIDO A TRATAMIENTO CRóNICO CON CORTISOL
C. M. Cortez,W. S. Costa,M. A. Babinski,M. A. Chagas
International Journal of Morphology , 2003,
Abstract: The present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. The study was based on morphometric and stereological analysis of the perikarya. Eight rats were treated intraperitoneally with cortisol (1mg/Kg/day) during 36 days. Treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. There was a significant difference (<=P0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). The decrease of perikarya neuronal diameter was also demonstrated by stereological methods. Morphometrical findings may suggest alterations in superior cervical ganglion neuronal activity in rats treated for long term with cortisol Se investigó la acción de corticoides en el crecimiento del ganglio cervical superior de rata y se describieron los efectos del cortisol durante las fases tempranas de desarrollo. El estudio se basó en los análisis morfométrico y estereológico del pericario. Fueron inyectadas intraperitonealmente 8 ratas con cortisol (1mg/Kg/día) durante 36 días. El tratamiento se comenzó en el día 8 después del nacimiento, finalizándose un día antes del sacrificio. Hubo una diferencia significativa (<=P0,05) en el promedio del diámetro neuronal entre el grupo control (16.78 ± 1.11 mm) y los animales tratados (15.84 ± 0.99 mm). La disminución del diámetro neuronal del pericario también fue demostrada a través de la estereología. Los hallazgos morfométricos pueden hacer pensar en alteraciones de la actividad neuronal del ganglio cervical superior, en ratas tratadas por un largo periodo con cortisol
Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion Características Histoquímicas de la Matriz Extracelular en el Ganglio ótico de Rattus rattus norvegicus
M. A. Chagas,M. A. Babinski,M. Abidu-Figueiredo,W. S. Costa
International Journal of Morphology , 2006,
Abstract: The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 μm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS. The presence of neutral glycoproteins was demonstrated by PAS reactivity. PAS inhibition following Alcian Blue staining in pH 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. The extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. These findings suggests a mutual interaction and a complex role in ganglionic physiology El ganglio ótico es un componente craneal de la división parasimpática del sistema nervioso autónomo (SNA). Similar al otro ganglio parasimpático, el ganglio ótico presenta neuronas multipolares que están rodeadas totalmente por las células satélites y la sustancia intercelular, lo cual permite la utilización de este ganglio como un buen modelo experimental para estudiar las relaciones matriz extracelular/neurona. Examinamos, con microscopio de luz, el ganglio ótico 10 ratones. Con los métodos histológicos rutinarios fueron obtenidas 5 secciones y coloreadas con tricrómico de Gomori, PAS, Azul de Alcián pH 2.5 y pH1.0, acetilación + PAS, acetilación + desacetilación + PAS, hidrólisis de ácido + PAS, fenilhidrazina + PAS y tiosemicarbacida + PAS. La presencia de glicoproteínas neutras fue demostrada por la reactividad de PAS. La inhibición de PAS y la posterior tinción con Azul de Alcian en pH 2.5 y 1.0, demostró la presencia de una cantidad peque a de glicoproteínas ácidas. El análisis extracelular de la matriz demostró la presencia de glicoconjugados neutros y ácidos. Estos resultados sugieren una interacción mutua y un papel complejo en la fisiología ganglionar
Biological and physicochemical properties of cowpea severe mosaic comovirus isolated from soybean in the State of Paraná
Bertacini, Paula V.;Almeida, álvaro M.R.;Lima, J. Albérsio A.;Chagas, Cesar M.;
Brazilian Archives of Biology and Technology , 1998, DOI: 10.1590/S1516-89131998000400004
Abstract: soybean plants with symptoms of bud blight were growing close to cowpea with severe symptoms of mosaic associated with blisters in the leaves. a group of plants of both species were collected and used for etiological studies. this kind of symptom in soybeans was common in certain areas of the state of paraná, induced by tobacco streak ilarvirus. host range, serological reaction, particle morphology and size, protein and nucleic acid analysis, and transmission by beetles from species cerotoma arcuata oliv. showed that the virus involved was cowpea severe mosaic comovirus. this is the first report on the occurrence of this virus in soybean plants in the state of paraná. results using indirect elisa showed that in cowpea the relative virus concentration was higher in green leaf areas than in chlorotic ones. also, virus concentration, determined through indirect elisa was much higher in plants kept at diurnal regime of 25o c x 23o c (12 x 12 h) than at 30o c x 28o c.
Exposure to flaxseed during lactation does not alter prostate area or epithelium height but changes lipid profile in rats Exposición a la linaza durante la lactación no cambia el área o altura epitelial de la próstata pero cambia el perfil lipídico en ratones
L. Ferreira Medeiros de Fran?a Cardozo,M. Alves Chagas,L. Leal Soares,A. Andrade Troina
Nutrición Hospitalaria , 2010,
Abstract: Flaxseed intake has increased owing to beneficial effects to health and prevention of diseases. Provided that it's an important source of lignan, a phytoestrogen, the present study aimed at evaluating the possible effect of the intake of this seed during lactation upon prostate, sexual hormones and lipidic profile of the offspring in adult life. Material and methods: 16 female Wistar rats were used. After delivery, they were divided into two different groups to receive one of the following diets during lactation: Control group (CG), with a casein based diet and Flaxseed group (FG), with a flaxseed based diet containing 25% flaxseed. At weaning, male pups received commercial chow until adult life (170 days old), when they were sacrificed. Results: No differences were perceived concerning offspring food intake and body weight at 170 days. There was a reduction in total cholesterol levels (FG = 45.71 ± 8.96 mg/dL; CG = 63.43 ± 15.69 mg/dL, p = 0.02) and triglycerides (FG = 54.29 ± 11.10 mg/dL; CG = 79.86 ± 25.68 mg/dL, p = 0.03). Also, no alterations were observed in prostatic morphology, testosterone or estradiol levelsin the two groups analyzed. Conclusion: Flaxseed intake during lactation did not produce histological alterations in prostatic alveolus or in sexual hormones, but programmed to a reduction in lipid profile in adult life with decreased cardiovascular risk. El consumo de la linaza ha aumentado debido a los efectos beneficiosos para la salud y la prevención de enfermedades. Siendo una importante fuente de lignanos, un fitoestrógeno, el presente estudio evaluó los efectos de la administración de esta semilla durante la lactación en la próstata, las hormonas sexuales y perfil lipídico de loshijos en la vida adulta. Material y métodos: Fueron utilizados 16 ratones Wistar hembras. Después del parto fueron divididas en dos grupos recibiendo durante la lactación las siguientes dietas: Grupo Control (GC), con ración a base de caseína y Grupo Linaza (GL), con ración a base de caseína conteniendo 25% de semilla de linaza. En el destete, las crías machos pasaron a recibir ración comercial hasta la edad adulta, cuando fueron muertos a los 170 días de vida. Resultados: No fueron verificadas diferencias sobre el consumo alimentario y peso corporal de los animales a los 170 días. Hubo una reducción en los niveles de colesterol total (GL = 45,71 ± 8,96 mg/dL; GC = 63,43 ± 15,69 mg/dL, p = 0,02) y triglicéridos (GL = 54,29 ± 11,10 mg/dL; GC = 79,86 ± 25,68 mg/dL, p = 0,03). Además, no se observaron alteraciones en la morfología de la próstata, la testosterona
LA RELACIóN DE LOS ESTUDIANTES CON EL CADáVER EN EL ESTUDIO PRáCTICO DE ANATOMíA: LA REACCIóN E INFLUENCIA EN EL APRENDIZAJE
Babinski,M. A.; Sgrott,E. A.; Luz,H. P.; Brasil,F. B.; Chagas,M. A.; Abidu-Figueiredo,M.;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000200007
Abstract: theviewandhandlingofcorpseinanatomy′sclassesarestillmethodsthatcontributeinanaffecientway totheform,espaceanddistancecomprehensionofanatomic′sestructure. nevertheless,thecontactwith corpse seemsto provokedistinctstypesofreactionsinstudents. purpose:identifythehealthstudernt′sreactionfacetoafirstcorpsecontactinanatomy′spracticeclasses. the application analyze theinfluenceofapracticeclassinanatomy′s"teaching and learning"too. 395healthstudents,whomtakepartinto teoricand practiceanatomy′sclasses, wereinterviewedby theendofthesemester. the instrumentofresearchissettleofaquestionnairewithclosedandfree inquiries puttinginto verse sex,age,course, interesting indisciplineandreactionsfacetoacorpsecontact.thestatistictreatment ofdatawasdonehelppedbygraph padinstat software, whereinthechoosentestwasqi2 withap<0.05likesignificative. mostofthemwereyoung,being themajorityfeminine.92,2%ofthestudents consider"important" andarereallyinterestedin takenoticeabouthumanbody independentofcurricular exigence. thereactionfacetoafirstcorpsecontact,40,8% reported nonefear.somereactionswereidentifiedin16%asfear,nausea,shockand recallto memoryfromdeceasedrelatives."familiarity" with the corpse,thestudents revealed anacademical relation,respectingyourstudy instrumentandshowedthatthey don′tsuffer any quotidian variation innourishment,slee,etc. consideringthepractice studyincorpse, itwas"very important"whereas itshandling influencein apprenticeship. seemstooccuran"disfigured"ofthecorpseafterdissection,becausethe reactionsfacetoafirst contactwerenot expressives. thepracticestudyin corpseisindispensablesinceit materializesandcorrects wrong teorics conceitsand/orvariations upontopographicsaspects.
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