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Search Results: 1 - 10 of 714318 matches for " M. A. Babinski "
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Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion
Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S.;
International Journal of Morphology , 2006, DOI: 10.4067/S0717-95022006000100015
Abstract: the otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ans). similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. we have studied rat otic ganglion in 10 animals through light microscopy. after routine histological methods, 5 μm sections were obtained and coloured by gomori`s trichrome, periodic acid-schiff (pas), alcian blue ph 2.5 and ph1.0, acetylation + pas, acetylation + deacetylation + pas, acid hydrolysis + pas, phenylhydrazine + pas and thiosemicarbazide + pas. the presence of neutral glycoproteins was demonstrated by pas reactivity. pas inhibition following alcian blue staining in ph 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. the extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. these findings suggests a mutual interaction and a complex role in ganglionic physiology
MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL
Cortez,C. M.; Costa,W. S.; Babinski,M. A.; Chagas,M. A.;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000300006
Abstract: the present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. the study was based on morphometric and stereological analysis of the perikarya. eight rats were treated intraperitoneally with cortisol (1mg/kg/day) during 36 days. treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. there was a significant difference (£p0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). the decrease of perikarya neuronal diameter was also demonstrated by stereological methods. morphometrical findings may suggest alterations in superior cervical ganglion neuronal activity in rats treated for long term with cortisol
MORFOLOGíA Y FRACCIóN DEL áREA DEL LUMEN GLANDULAR DE LA ZONA DE TRANSICIóN EN LA PRóSTATA HUMANA
Babinski,M. A.; Chagas,M. A.; Costa,W.S.; Pereira,M. J.;
Revista chilena de anatomía , 2002, DOI: 10.4067/S0716-98682002000300004
Abstract: although the male reproductive system diseases are not restricted to the prostate, this is one of the most interesting organs for the urologic medical practice. the benign prostatic hyperplasia (bph) represents a tissual increase of these histological elements causing profound alterations in the prostate organisation. therefore this study aims at evaluating the glandular lumen volumetric density (vv) the transition zone (median lobe) in human normal prostates compared with hyperplastic ones (bph). controls consisted of 10 samples of prostates transition region obtained during necropsy of young adults victims of accidents. samples of prostatic tissue of the transition zone were obtained from 10 patients with clinical symptoms of bladder outlet obstruction and histopathological diagnoses of the bph submitted to transvesicalprostatectomy. samples of the 5mm were stained on paraffin sections of prostate using hematoxilin-eosin. for the quantitative analysis the stereology method assisted by a software program was used to verify the vv. the glandular lumen volumetric density showed the significant statistically bph increase, which seems to be related to the bladder outlet obstruction and to the stasis of the luminal contents, causing intraluminal pressure in turn deformations in the glandular with probable consequences in the epithelium, well the to the secretory alterations.
MORFOLOGíA Y FRACCIóN DEL áREA DEL LUMEN GLANDULAR DE LA ZONA DE TRANSICIóN EN LA PRóSTATA HUMANA MORPHOLOGY AND AREAL FRACTION OF THE GLANDULAR LUMEN OF TRANSITION ZONE IN THE HUMAN PROSTATE
M. A. Babinski,M. A. Chagas,W.S. Costa,M. J. Pereira
Revista chilena de anatomía , 2002,
Abstract: Aunque las enfermedades del sistema reproductor masculino no se restringen a la próstata, éste es uno de los órganos más interesantes para la práctica médica urológica. La hiperplasia prostática benigna (HPB) representa un aumento tisular de estos elementos histológicos que causan alteraciones profundas en el organización de la próstata. Este estudio pretende evaluar la densidad volumétrica (VV) del lumen glandular de la zona de transición (lóbulo mediano) en próstatas humanas normales, comparadas con hiperplásicas (HPB). Muestras de tejidos prostáticos de la zona de transición fueron obtenidas de 10 pacientes con síntomas de obstrucción infravesical y diagnóstico histopatológico del HPB, sometidos a prostatectomía transvesical. Para control fueron utilizadas 10 muestras de la región de transición prostática, obtenidas durante la necroscopía de jóvenes adultos, víctimas de accidentes. Los bloques de parafina de muestras prostáticas fueron seccionados con 5μm de grosor y te idos con Hematoxilina y Eosina. Para el análisis cuantitativo, fue usado el método estereológico asistido por un programa de software para verificar la VV. La densidad volumétrica del lumen glandular, mostró un incremento estadísticamente significativo de la HPB, y esto parece estar relacionado con la obstrucción infravesical y el éstasis del contenido luminal, aumentando la presión intraluminal y, por tanto, causa deformaciones de las glándulas con probables consecuencias en el epitelio, así como alteraciones secretorias. Although the male reproductive system diseases are not restricted to the prostate, this is one of the most interesting organs for the urologic medical practice. The benign prostatic hyperplasia (BPH) represents a tissual increase of these histological elements causing profound alterations in the prostate organisation. Therefore this study aims at evaluating the glandular lumen volumetric density (VV) the transition zone (median lobe) in human normal prostates compared with hyperplastic ones (BPH). Controls consisted of 10 samples of prostates transition region obtained during necropsy of young adults victims of accidents. Samples of prostatic tissue of the transition zone were obtained from 10 patients with clinical symptoms of bladder outlet obstruction and histopathological diagnoses of the BPH submitted to transvesicalprostatectomy. Samples of the 5mm were stained on paraffin sections of prostate using hematoxilin-eosin. For the quantitative analysis the stereology method assisted by a software program was used to verify the VV. The glandular lumen volumetric den
MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL ANáLISIS MORFOMéTRICO Y ESTEREOLóGICO DEL GANGLIO CERVICAL SUPERIOR DE Rattus norvegicus SOMETIDO A TRATAMIENTO CRóNICO CON CORTISOL
C. M. Cortez,W. S. Costa,M. A. Babinski,M. A. Chagas
International Journal of Morphology , 2003,
Abstract: The present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. The study was based on morphometric and stereological analysis of the perikarya. Eight rats were treated intraperitoneally with cortisol (1mg/Kg/day) during 36 days. Treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. There was a significant difference (<=P0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). The decrease of perikarya neuronal diameter was also demonstrated by stereological methods. Morphometrical findings may suggest alterations in superior cervical ganglion neuronal activity in rats treated for long term with cortisol Se investigó la acción de corticoides en el crecimiento del ganglio cervical superior de rata y se describieron los efectos del cortisol durante las fases tempranas de desarrollo. El estudio se basó en los análisis morfométrico y estereológico del pericario. Fueron inyectadas intraperitonealmente 8 ratas con cortisol (1mg/Kg/día) durante 36 días. El tratamiento se comenzó en el día 8 después del nacimiento, finalizándose un día antes del sacrificio. Hubo una diferencia significativa (<=P0,05) en el promedio del diámetro neuronal entre el grupo control (16.78 ± 1.11 mm) y los animales tratados (15.84 ± 0.99 mm). La disminución del diámetro neuronal del pericario también fue demostrada a través de la estereología. Los hallazgos morfométricos pueden hacer pensar en alteraciones de la actividad neuronal del ganglio cervical superior, en ratas tratadas por un largo periodo con cortisol
Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion Características Histoquímicas de la Matriz Extracelular en el Ganglio ótico de Rattus rattus norvegicus
M. A. Chagas,M. A. Babinski,M. Abidu-Figueiredo,W. S. Costa
International Journal of Morphology , 2006,
Abstract: The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 μm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide + PAS. The presence of neutral glycoproteins was demonstrated by PAS reactivity. PAS inhibition following Alcian Blue staining in pH 2.5 and 1.0 showed the presence of a small quantity of acid glycoprotein. The extracellular matrix analysis showed the presence of neutral and acid glycoconjugates. These findings suggests a mutual interaction and a complex role in ganglionic physiology El ganglio ótico es un componente craneal de la división parasimpática del sistema nervioso autónomo (SNA). Similar al otro ganglio parasimpático, el ganglio ótico presenta neuronas multipolares que están rodeadas totalmente por las células satélites y la sustancia intercelular, lo cual permite la utilización de este ganglio como un buen modelo experimental para estudiar las relaciones matriz extracelular/neurona. Examinamos, con microscopio de luz, el ganglio ótico 10 ratones. Con los métodos histológicos rutinarios fueron obtenidas 5 secciones y coloreadas con tricrómico de Gomori, PAS, Azul de Alcián pH 2.5 y pH1.0, acetilación + PAS, acetilación + desacetilación + PAS, hidrólisis de ácido + PAS, fenilhidrazina + PAS y tiosemicarbacida + PAS. La presencia de glicoproteínas neutras fue demostrada por la reactividad de PAS. La inhibición de PAS y la posterior tinción con Azul de Alcian en pH 2.5 y 1.0, demostró la presencia de una cantidad peque a de glicoproteínas ácidas. El análisis extracelular de la matriz demostró la presencia de glicoconjugados neutros y ácidos. Estos resultados sugieren una interacción mutua y un papel complejo en la fisiología ganglionar
Quadexciton cascade and fine structure splitting of the triexciton in a single quantum dot
M. R. Molas,A. A. L. Nicolet,A. Babinski,M. Potemski
Physics , 2015,
Abstract: We report the properties of emission lines associated with the cascaded recombination of a quadexciton in single GaAlAs/AlAs quantum dots, studied by means of polarization-resolved photoluminescence and single-photon correlation experiments. It is found that photons which are emitted in a double-step 4X-3X process preserve their linear polarization, similarly to the case of conserved polarization of correlated photons in the 2X-X cascade. In contrast, an emission of either co-linear or cross-linear pairs of photons is observed for the 3X-2X cascade. Each emission line associated with the quadexciton cascade shows doublet structure in the polarization-resolved photoluminescence experiment. The maximum splitting is seen when the polarization axis is chosen along and perpendicular to the [110] crystallographic direction. This effect is ascribed to the fine structure splitting of the exciton and triexciton states in the presence of an anisotropic confining potential of ae dot. We also show that the splitting in the triexciton state surpasses that in the exciton state by a factor up to eight and their ratio scales with the energy distance between the 3X and X emission lines, thus, very likely, with a lateral size and/or a composition of the dot.
A Rare Variation of the Retro-Aortic Left Renal Vein with Anastomotic Afluent from Inferior Mesenteric Vein
Andrade,F. M.; Rocha,R. P.; Pereira,H. M.; Fernandes,R. M. P.; Babinski,M. A.;
International Journal of Morphology , 2005, DOI: 10.4067/S0717-95022005000100001
Abstract: we report an uncommon anatomical variations of the left renal vein was found on dissected specimen in an elderly male cadaver: a retroaortic left renal vein. no other vascular anomalies were noted in this specimen. the anomaly result may be related to a particular pattern of left inferior vena cava. the abnormality have to be known for it may be undetected or be misleading in imaging. this anatomical curiosity should be kept in mind by clinicians and academics that may manipulate this anatomical area
VARIACIONES ANATóMICAS ENTRE EL NERVIO ISQUIáTICO Y EL MúSCULO PIRIFORME DURANTE EL PERíODO FETAL HUMANO ANATOMICAL VARIATIONS BETWEEN SCIATIC NERVE AND PIRIFORM MUSCLE DURING FETAL PERIOD IN HUMAN
F. A. Machado,M. A. Babinski,F. B. Brasil,L. A. Favorito
International Journal of Morphology , 2003,
Abstract: El nervio isquiático, ramo del plexo sacro, abandona la pelvis pasando, generalmente, por debajo del músculo piriforme. Este nervio puede presentar variaciones en su relación con el músculo piriforme, entre ellas, las referidas a la división alta. Sin embargo, son pocos los trabajos que correlacionan esta división con variaciones anatómicas y su relación con el músculo piriforme, en fetos humanos. Con el propósito de verificar una posible asociación entre el trayecto y relaciones de ramos del nervio isquiático, fueron disecadas 100 regiones glúteas de fetos humanos. Se encontraron 3 tipos de variaciones entre el nervio isquiático y el músculo piriforme: en 8 casos (16,0 %) el nervio fibular común pasó a través del músculo y el nervio tibial transcurrió por debajo de su margen inferior (Tipo I). En estos mismos casos se observó que 5 (62,5%) estaban a la izquierda y 3 a la derecha. En un caso (2,0 %), el nervio fibular común hizo su trayecto por sobre el margen superior del músculo piriforme y el nervio tibial pasó por debajo de su margen inferior (Tipo II). Fue observado en el Tipo III,que el nervio isquiático perfora el músculo piriforme, no presentando ninguna división. El músculo piriforme mostró variaciones de fusión con el músculo glúteo mediano (lado derecho =2,0%) y con el músculo gemelo superior (lado derecho =24,0% e izquierdo =32,0%). Estos datos muestran que existe una equivalencia de variacion entre los trayectos y relaciones del nervio isquiático con el músculo piriforme en fetos y adultos. Las variaciones en el músculo piriforme no están asociadas con alteraciones funcionales en la biomecánica de la pelvis. The sciatic nerve, branch of the sacral plexus, leaves the pelvis and passes usually below of the piriform muscle. The sciatic nerve also comes from this plexus and can have distinct relationships with the mentioned muscle, as the high division of the nerve. However, there are few studies that correlate that division with the course of the sciatic nerve and its relationship with the piriform muscle, as well as, variations of that muscle in human fetuse. In order to verify a possible association between those anatomical structures, we dissected 100 gluteal regions of human fetuses. Three types of variations were found between the sciatic nerve and the piriform muscle. The common fibular nerve pierced the muscle and the tibial nerve passed below of its inferior margin in 8 cases (16,0 %), corresponding to the type I. In these cases, we found 62,5% in right side and 37,5% in left side. The type II was observed in 1 case (2,0 %), the common
Optical readout of charge and spin in a self-assembled quantum dot in a strong magnetic field
M. Korkusinski,P. Hawrylak,A. Babinski,M. Potemski,S. Raymond,Z. Wasilewski
Physics , 2007, DOI: 10.1209/0295-5075/79/47005
Abstract: We present a theory and experiment demonstrating optical readout of charge and spin in a single InAs/GaAs self-assembled quantum dot. By applying a magnetic field we create the filling factor 2 quantum Hall singlet phase of the charged exciton. Increasing or decreasing the magnetic field leads to electronic spin-flip transitions and increasing spin polarization. The increasing total spin of electrons appears as a manifold of closely spaced emission lines, while spin flips appear as discontinuities of emission lines. The number of multiplets and discontinuities measures the number of carriers and their spin. We present a complete analysis of the emission spectrum of a single quantum dot with N=4 electrons and a single hole, calculated and measured in magnetic fields up to 23 Tesla.
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