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Search Results: 1 - 10 of 484412 matches for " Mónica Carre?o "
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Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias Evaluation of molecular and immunoenzymatic assays for detecting Enterohemorrhagic E coli in food borne outbreaks
Maricel Vidal O,Mónica Carreo C,Roberto Vidal A,Carolina Arellano C
Revista médica de Chile , 2002,
Abstract: Background: Enterohemorrhagic Escherichia coli (EHEC), is an emergent pathogen that causes sporadic infections and outbreaks of gastroenteritis associated with consumption of contaminated food products. Because detection of EHEC in diarrhea patients is not routinely performed, infection is most probably underestimated. Aim: To compare three techniques to detect EHEC: Colony hybridization with DNA probes, polymerase chain reaction (PCR) for the detection of stx1 and stx2 genes and immunoenzymatic detection by ELISA (Premier EHEC) of Stx1 and Stx2 toxins. Material and methods: Four outbreaks of food-borne gastroenteritis were studied including 16 patients and 78 strains of E coli. Twenty one (26,9%) strains, hybridized with the stx1 probe, 1 (1,3%) hybridized only with the stx2 probe and 36 (46,1%) with both probes. PCR amplification for cytotoxin genes was observed in 6 strains (7,7%) from the second outbreak studied. The immunoenzimatic assay detected the cytotoxins in 18 (23,0%), of the 78 studied strains. Agreement between probes and ELISA was 44,8%, between PCR and probes 34,7% and 82,4% between ELISA and PCR. Conclusions: These results indicate a variable yield among different EHEC detection techniques. Considering PCR as the gold standard, ELISA technique showed a better sensitivity and specificity than probes (Rev Méd Chile 2002; 130: 603-9)
Descripción clínica y epidemiológica de un grave brote de salmonelosis transmitida por alimentos
García-Huidobro,Diego; Carreo,Mónica; Alcayaga,Sergio; Ulloa,Jenny;
Revista chilena de infectología , 2012, DOI: 10.4067/S0716-10182012000200002
Abstract: background: foodborne diseases have increased considerably. aim: to report a foodborne outbreak, remarking the importance of early notification to activate the epidemiological surveillance system. results: during february 2011 we observed a salmonella enteritidis outbreak. 31.6% of the cases were seen in the same emergency care unit where all required intravenous fluid rehydration, and 41.7% were hospitalized because of severe dehydration. in the emergency room 45.5% of cases required a second visit to be diagnosed correctly. discussion: physicians under report the cases of this disease, delaying the activation of the epidemiological surveillance system. conclusions: besides providing good treatment to patients, physicians need to be qualified to recognize foodborne diseases and communicate early the suspicion of an outbreak to the epidemiological surveillance system in order to prevent new cases of disease in the community.
Descripción clínica y epidemiológica de un grave brote de salmonelosis transmitida por alimentos Clinical and epidemiological description of severe outbreak of foodborne infection by Salmonella Enteritidis
Diego García-Huidobro,Mónica Carreo,Sergio Alcayaga,Jenny Ulloa
Revista chilena de infectología , 2012,
Abstract: Introducción: Las enfermedades transmitidas por alimentos (ETA) han aumentado considerablemente. Objetivo: Reportar un grave brote de ETA destacando la importancia de la notificación precoz para la activación del sistema de vigilancia epidemiológica. Resultados: Durante febrero de 2011 se observó un brote de Salmonella Enteritidis. Un 31,6% de los casos fueron atendidos en un mismo servicio de urgencia, donde todos requirieron la administración de fluidos endovenosos y 41,7% fueron hospitalizados por deshidratación grave. El 45,5% de los casos necesitó de una segunda consulta para ser diagnosticados correctamente. Discusión: La identificación de pacientes integrantes de un brote de ETA es difícil en los servicios de urgencia y los médicos sub-reportan los casos, retrasando al sistema de vigilancia epidemiológica. Conclusiones: Junto con brindar un adecuado tratamiento, los médicos deben estar capacitados para reconocer las ETA y comunicar tempranamente la sospecha de un brote para prevenir nuevos casos en la comunidad. Background: Foodborne diseases have increased considerably. Aim: To report a foodborne outbreak, remarking the importance of early notification to activate the epidemiological surveillance system. Results: During February 2011 we observed a Salmonella Enteritidis outbreak. 31.6% of the cases were seen in the same Emergency Care Unit where all required intravenous fluid rehydration, and 41.7% were hospitalized because of severe dehydration. In the Emergency Room 45.5% of cases required a second visit to be diagnosed correctly. Discussion: Physicians under report the cases of this disease, delaying the activation of the epidemiological surveillance system. Conclusions: Besides providing good treatment to patients, physicians need to be qualified to recognize foodborne diseases and communicate early the suspicion of an outbreak to the epidemiological surveillance system in order to prevent new cases of disease in the community.
Asociación entre el fenotipo fisura labiopalatina no sindrómica y marcadores de microsatélite ubicados en 4q Association between nonsyndromic cleft lip/palate with microsatellite markers located in 4q
Mónica Paredes A,Hernán Carreo Z,José Antonio Solá A,Jorge Segú C
Revista médica de Chile , 1999,
Abstract: Background: Nonsyndromic cleft lip with or without cleft palate (NSCLP) is a common craniofacial defect. Association studies have suggested that a clefting locus is located on chromosome 4q at or near two microsatellite markers D4S175 and D4S192. Aim: To test the hypothesis on the possible presence of a clefting locus on chromosome 4q. Material and methods: We carried out an association study on a sample of unrelated NSCLP patients, of their unaffected relatives and in controls. Both probands and relatives were further analyzed depending if they originated from simplex or multiplex families. DNA was analyzed with two PCR markers close to the putative NSCLP locus, dinucleotide repeats D4S175 and D4S192. PCR products were resolved by PAGE and visualized by silver staining. Statistical analysis was performed by means of c2 log ratio. Results: Significant differences between NSCLP and controls were observed when comparing the allele frequency distribution of D4S192 both in the total sample as well as in NSCLP-multiplex and simplex cases. No significant differences for D4S175 were observed in any of the comparisons. Unaffected relatives showed significant differences with controls both for D4S175 and D4S192. Conclusions: Our results support the hypothesis that a NSCLP locus maps on chromosome 4q close to the microsatellite marker D4S192. No differences were observed between NSCLP multiplex and simplex cases versus controls, implying that they do not represent different etiologic entities. The results of the present and previous studies in the same group of patients support the hypothesis that several major interacting genes participate in the etiology of NSCLP.
Asociación entre el fenotipo fisura labiopalatina no sindrómica y marcadores de microsatélites ubicados en 6p, 17q y 19q Association study of the Nonsyndromic Cleft Lip/Palate phenotype and microsatellite markers located in 6p, 17q and 19q
Hernán Carreo Z,José Suazo S,Mónica Paredes A,José Solá A
Revista médica de Chile , 2002,
Abstract: Background: In the search of the major genes responsible for the genetic etiology of Nonsyndromic Cleft Lip and Palate (NSCLP), an association study between this malformation and four molecular markers, F13A1 and EDN1 (6p), D17S579 (17q) and BCL3 (19q), was done. Aim: To determine, in a Chilean population, the presence of NSCLP susceptibility regions, as proposed for Caucasian populations in the 6p, 17q and 19q chromosomal regions. Material and Methods: A sample of unrelated NSCLP patients, that belonged to Simplex (Sx) and Multiplex (Mx) families, was analyzed. Blood donors were used as a control group (Co). The DNA of the four markers was amplified by means of PCR, their products analyzed by PAGE denaturants and visualized by silver staining. Statistical analysis was performed using chi2 log ratio. Results: Allele frequency distribution of D17S579 was significantly different in all patients with NSCLP and their subgroups, when compared to control subjects. Significant differences in EDN1 frecuency were observed between the total groups of NSCLP patients and those pertaining to the Mx subgroup, when compared to controls. Differences in F13A1 distribution were only observed between NSCLP-Mx patients and controls. There was a slight difference in BCL3 distribution, between the total sample of NSCLP patients and controls. Conclusions: Our results support the hypothesis of the existence of cleft susceptibility regions in 6p and 17q. The small significance of BCL3, suggests that ethnicity can influence the interactions between involved genes (Rev Méd Chile 2002; 130: 35-44)
Estudio de asociación entre la fisura labiopalatina no sindrómica y marcadores de microsatélite ubicados en 6p Association between non syndromic cleft lip palate and microsatellite markers located in 6p
Hernán Carreo Z,Mónica Paredes A,Gonzalo Téllez,Hernán Palomino Z
Revista médica de Chile , 1999,
Abstract: Background: Nonsyndromic cleft lip with or without cleft palate (NSCLP) is a common craniofacial developmental defect. Association studies have suggested that a clefting locus is located on chromosome 6p at or near two possible loci, Factor 13A (FI3A) in the region 6p 25-24 and HLA at 6p 21.3. Aim. To test the hypothesis on the possible presence of a major gene on chromosome 6p associated with NSCLP. Patients and methods: We carried out an association study on a sample of unrelated NSCLP patients from multiplex (Mx) and simplex (Sx) families, of their unaffected relatives and in control individuals. DNA was analyzed with three PCR markers close to the putative NSCLP locus, dinucleotide repeats at loci D6S89, D6S109 and D6S105. PCR products were resolved by PAGE and visualized by silver staining. Statistical analysis was performed by means of c2 log ratio. Results: Significant differences were observed when comparing the allele frequency distribution of D6S89 in patients with NSCLP and controls and in patients with NSCLP-Mx and controls. No significant differences were observed for patients with NSCLP-Sx. D6S109 and D6S105 showed no significant differences in any of the comparisons. Conclusions: Our results support the hypothesis that a NSCLP locus maps on 6p23 very close to D6S89. Results for D6S109 and D6S105 do not show a clear association. Differences observed between NSCLP-MX and Sx families seem to represent different etiologic entities. The results of the present study, plus those already published for candidate loci, TGFA and MSX1, support the hypothesis that several interacting major genes participate in the etiology of NSCLP.
Estudio de asociación entre la fisura labiopalatina no sindrómica y marcadores de microsatélite ubicados en 6p
Carreo Z,Hernán; Paredes A,Mónica; Téllez,Gonzalo; Palomino Z,Hernán; Blanco C,Rafael;
Revista médica de Chile , 1999, DOI: 10.4067/S0034-98871999001000006
Abstract: background: nonsyndromic cleft lip with or without cleft palate (nsclp) is a common craniofacial developmental defect. association studies have suggested that a clefting locus is located on chromosome 6p at or near two possible loci, factor 13a (fi3a) in the region 6p 25-24 and hla at 6p 21.3. aim. to test the hypothesis on the possible presence of a major gene on chromosome 6p associated with nsclp. patients and methods: we carried out an association study on a sample of unrelated nsclp patients from multiplex (mx) and simplex (sx) families, of their unaffected relatives and in control individuals. dna was analyzed with three pcr markers close to the putative nsclp locus, dinucleotide repeats at loci d6s89, d6s109 and d6s105. pcr products were resolved by page and visualized by silver staining. statistical analysis was performed by means of c2 log ratio. results: significant differences were observed when comparing the allele frequency distribution of d6s89 in patients with nsclp and controls and in patients with nsclp-mx and controls. no significant differences were observed for patients with nsclp-sx. d6s109 and d6s105 showed no significant differences in any of the comparisons. conclusions: our results support the hypothesis that a nsclp locus maps on 6p23 very close to d6s89. results for d6s109 and d6s105 do not show a clear association. differences observed between nsclp-mx and sx families seem to represent different etiologic entities. the results of the present study, plus those already published for candidate loci, tgfa and msx1, support the hypothesis that several interacting major genes participate in the etiology of nsclp.
Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
Vidal O,Maricel; Carreo C,Mónica; Vidal A,Roberto; Arellano C,Carolina; Solari G,Verónica; Prado J,Valeria;
Revista médica de Chile , 2002, DOI: 10.4067/S0034-98872002000600001
Abstract: background: enterohemorrhagic escherichia coli (ehec), is an emergent pathogen that causes sporadic infections and outbreaks of gastroenteritis associated with consumption of contaminated food products. because detection of ehec in diarrhea patients is not routinely performed, infection is most probably underestimated. aim: to compare three techniques to detect ehec: colony hybridization with dna probes, polymerase chain reaction (pcr) for the detection of stx1 and stx2 genes and immunoenzymatic detection by elisa (premier ehec) of stx1 and stx2 toxins. material and methods: four outbreaks of food-borne gastroenteritis were studied including 16 patients and 78 strains of e coli. twenty one (26,9%) strains, hybridized with the stx1 probe, 1 (1,3%) hybridized only with the stx2 probe and 36 (46,1%) with both probes. pcr amplification for cytotoxin genes was observed in 6 strains (7,7%) from the second outbreak studied. the immunoenzimatic assay detected the cytotoxins in 18 (23,0%), of the 78 studied strains. agreement between probes and elisa was 44,8%, between pcr and probes 34,7% and 82,4% between elisa and pcr. conclusions: these results indicate a variable yield among different ehec detection techniques. considering pcr as the gold standard, elisa technique showed a better sensitivity and specificity than probes (rev méd chile 2002; 130: 603-9)
Complex segregation analysis of nonsyndromic cleft lip/palate in a Chilean population
Blanco, Rafael;Arcos-Burgos, Mauricio;Paredes, Mónica;Palomino, Hernán;Jara, Lilian;Carreo, Hernán;Obreque, Victor;Mu?oz, M.A.;
Genetics and Molecular Biology , 1998, DOI: 10.1590/S1415-47571998000100023
Abstract: a popula??o urbana chilena contemporanea deriva da mistura de ameríndios nativos com espanhóis, apresentando uma incidência média de fissura labial n?o sindr?mica associada ou n?o a fissura palatina (nsclp) de 1,8 por 1000 nascimentos vivos. a análise de segrega??o complexa usando o programa de computador pointer foi feita em 249 pedigrees estendidos, distribuídos em 202 famílias simplex e 47 famílias multiplex obtidas de probands de nsclp afetados (157 homens e 92 mulheres). esses pedigrees deram origem a 326 indivíduos afetados e mais de 1454 parentes. oito modelos hipotéticos foram examinados e comparados pelo teste c 2 log2 raz?o de máxima verossimilhan?a. os modelos que postulam que nsclp n?o era transmitida nestas famílias foram rejeitados, assim como os modelos que postulam apenas um componente multifatorial (p < 0,0001). o modelo que postula n?o haver componente poligênico para a transmiss?o n?o p?de ser rejeitado mas o modelo de n?o transmiss?o do efeito mais importante foi rejeitado (p < 0,0001). entre os modelos do locus mais importante apenas o modelo recessivo de transmiss?o foi rejeitado, enquanto que as heran?as codominante e dominante sem um componente multifatorial n?o puderam ser excluídas. o modelo n?o restrito sugere que a freqüência do alelo de suscetibilidade a nsclp no locus mais importante é 0,0037 e sua penetrancia é de 92%.
Evaluación de la asociación entre marcadores de microsatélite en 6p22-25 y fisura labiopalatina no sindrómica utilizando el dise?o de tríos caso-progenitores en la población chilena
Blanco C,Rafael; Suazo S,José; Santos M,José Luis; Carreo Z,Hernán; Paredes A,Mónica; Jara S,Lilian; Eltit G,Felipe;
Revista médica de Chile , 2003, DOI: 10.4067/S0034-98872003000700008
Abstract: background: genetic studies indicate that nonsyndromic cleft lip/palate (nsclp) has the characteristics of a complex genetic trait. reports from different authors have suggested several candidate genes mapping in different chromosome regions. association studies have suggested that a clefting locus is located on chromosome 6p. on these grounds we have investigated the possible association between five microsatellite markers located on 6p22-25 and nsclp. aim: to test the hypothesis on the possible association of a clefting locus with microsatellite markers located in 6p22-25. patients and methods: the sample consisted of 54 unrelated case-parent trios that comprise 54 nsclp probands and 108 parents. five microsatellite markers spanning the region 6p22-25 were analyzed for each individual by means of polymerase chain reaction with fluorescent labeled microsatellite markers. electrophoresis of the pcr products was performed on a laser-fluorescent dna sequencer. nonparametric etdt and mcetdt programs, were used to analyze the genotype data. results: the family based association study showed that for the genotype wise analysis, only d6s259 presented a significant p-value (0.03). nevertheless no individual allele of this marker showed an evident preferential transmission from heterozygous parents to affected offspring. conclusions: the results of the present study do not show a clear evidence that a candidate gene for nsclp may be located within or near the analyzed chromosome region in our sample. nevertheless, it must be emphasized that the genotype wise analysis shows a significant p-value for d6s259 marker (rev méd chile 2003; 131: 765-72)
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