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Search Results: 1 - 10 of 402090 matches for " Márcia Luzia Ferrarezi Maluf "
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Inquérito epidemiológico sobre a paracoccidioidomicose utilizando a gp43 em dois municípios do noroeste do Paraná, Brasil
Fornajeiro, Nair;Maluf, Márcia Luzia Ferrarezi;Takahachi, Gisele;Svidzinski, Terezinha Inez Estivalet;
Revista da Sociedade Brasileira de Medicina Tropical , 2005, DOI: 10.1590/S0037-86822005000200014
Abstract: an epidemiological survey was performed in 118 workers from northwestern paraná state using gp43 as antigen, the positivity was 43%. this result, plus the weather conditions, which are favorable for fungus development, underscores that this region is an endemic area for paracoccidioidomycosis and is a reservoir of paracoccidioides brasiliensis.
Prevalência de paracoccidioidomicose-infec??o determinada através de teste sorológico em doadores de sangue na regi?o Noroeste do Paraná, Brasil
Maluf, Márcia Luzia Ferrarezi;Pereira, Sandra Regina Corbello;Takahachi, Gisele;Svidzinski, Terezinha Inez Estivalet;
Revista da Sociedade Brasileira de Medicina Tropical , 2003, DOI: 10.1590/S0037-86822003000100003
Abstract: in this paper the classical elisa test was used, which is a highly sensitive method, with the objective of estimating the anti-paracoccidioides brasiliensis antibodies level in healthy people, residing in supposedly endemic regions for pcm, northwest paraná state, brazil. the sera was analyzed from 680 blood donors' who were approved by the classical serologic screening by blood banks. four cities were studied (campo mour?o, maringá, cianorte and umuarama) with a sample of 170 donors each. the antigen used was ag7days, diluted serum 1/400, anti human igg-conjugate marked with peroxidase and revealed with o-phenylenediamine. it was possible to detect 181 (27%) sera with antibody level above the cut off threshold. the percentage varied between the cities. these results allied with the favorable climatic and geographical conditions and also previous reports of pcm indigenous to the region, indicate that the northwest of paraná is an endemic region for pcm.
Inquérito epidemiológico sobre a paracoccidioidomicose utilizando a gp43 em dois municípios do noroeste do Paraná, Brasil
Fornajeiro Nair,Maluf Márcia Luzia Ferrarezi,Takahachi Gisele,Svidzinski Terezinha Inez Estivalet
Revista da Sociedade Brasileira de Medicina Tropical , 2005,
Abstract: Foi realizado um inquérito epidemiológico sobre paracoccidioidomicose com gp43, em 118 operários no Noroeste do Paraná. A positividade foi de 43%, o que aliado às condi es ambientais favoráveis ao desenvolvimento do fungo refor am que essa regi o seja endêmica para paracoccidioidomicose e também reservárea de Paracoccidioides brasiliensis.
Prevalência de paracoccidioidomicose-infec o determinada através de teste sorológico em doadores de sangue na regi o Noroeste do Paraná, Brasil
Maluf Márcia Luzia Ferrarezi,Pereira Sandra Regina Corbello,Takahachi Gisele,Svidzinski Terezinha Inez Estivalet
Revista da Sociedade Brasileira de Medicina Tropical , 2003,
Abstract: No presente trabalho, empregamos uma metodologia de alta sensibilidade, o teste de ELISA clássico, com o objetivo de avaliar os níveis de anticorpos anti Paracoccidioides brasiliensis em indivíduos saudáveis, residentes na regi o Noroeste do Paraná. Foram analisados 680 soros de doadores de sangue aprovados pela triagem sorológica clássica de Bancos de Sangue. Os doadores eram residentes em quatro municípios. O exo-antígeno empregado foi o Ag7dias, soros diluídos 1/400, conjugado anti-IgG humana marcada com peroxidase e revelada com orto-fenilenodiamina. Foi possível detectar 181 (27%) soros com níveis de anticorpos acima do valor de corte. Essa porcentagem variou entre os municípios amostrados: Campo Mour o, Maringá, Cianorte e Umuarama. Nossos resultados, aliados às condi es climáticas e geográficas favoráveis, além de relatos anteriores de casos de PCM autóctones da regi o, permitem sugerir que a regi o Noroeste do Paraná seja regi o endêmica para PCM.
Prevalence of yeast species in the oral cavity and its relationship to dental caries = Prevalência de leveduras na cavidade bucal e sua rela o com a cárie dental
Adriane Castro Martinez Martins,Márcia Luzia Ferrarezi Maluf,Terezinha Inez Estivalet Svidzinski
Acta Scientiarum : Health Sciences , 2011,
Abstract: The purpose of this study was to evaluate the frequency of yeasts in the oral cavity, and to determine the main yeast species present and whether there is a correlation between the presence of yeasts in the mouth and caries or dental plaque. Ninety-five healthy children and adolescents who were residents of rural villages participated in the study. They were submitted to an odontological examination for the identification of dental caries and dental plaque, as well as for yeast culture and identification. The frequency of yeasts was 33.7%, with no difference between females and males. Yeasts predominated in children (37.7%) more than in adolescents (26.5%). Caries and dental plaque were present in 70.3 and 96.8% of the sample, respectively, and Candida albicans was the most prevalent species (75%). The findings indicated a correlation between the presence of caries and yeasts in the mouth (p = 0.0087) and between yeasts and the number of carious teeth (p < 0.05). However, there was no correlation betweenyeasts and the dental plaque index (p = 0.49). O objetivo deste estudo foi avaliar a frequência de leveduras na cavidade bucal, determinar as principais espécies de leveduras presentes e, se existe correla o entre a presen a de leveduras na boca com cárie ou placa dental. Participaram deste estudo 95 crian as e adolescentes saudáveis que residiam em Vilas Rurais, na regi o Oeste do Estado do Paraná. Eles foram submetidos a exame odontológico para identifica o de cárie e placa dental, além de pesquisa de levedura na cavidade bucal. A frequência de leveduras foi 33,7% e n o houve diferen a entre os gêneros femininos e masculinos. As leveduras predominaram nas crian as (37,7%) em rela o aos adolescentes (26,5%). Cárie e placa dental estavam presentes, respectivamente, em 70,3 e 96,8% da amostra e Candida albicans foi à espécie mais prevalente (75%). Os resultados mostraram correla o entre a presen a de cárie e de levedura na boca (p = 0,0087) e também entre a presen a de leveduras e o número de dentes cariados (p < 0,05). Por outro lado, n o houve correla o entre leveduras e índice de placa dental (p = 0,49).
Dy and umided salting of fillets of pacu (piaractus mesopotamicus) Salga seca e úmida de filés de pacu (piaractus mesopotamicus)
Jakeline Marcela Azambuja de Freitas,Letícia Hayashi Higuchi,Aldi Feiden,Márcia Luzia Ferrarezi Maluf
Semina : Ciências Agrárias , 2011,
Abstract: The goal of this study was to evaluate the process of salting and dry salting of fillets in pacu (P. mesopotamicus), from cultivation in cages. Fillet with skin were salted at a rate of 40% of salt in relation of the initial weight, for a period of 10 days. After processing, were evaluated for microbiological and centesimal composition of fillets subjected to salting and determining the concentration of sodium chloride (NaCl) and calculation of income. The centesimal composition were evaluated the protein, ether extract, moisture and ash of the raw material fresh and processed products by salting. The microbiological analysis included the determination of coliforms at 45 ° C, Salmonella and Staphylococcus coagulase positive. The pacus subjected to umided and dry salting were within quality control standards established by the legislation. The content of umided, protein and lipids and chlorides showed differences (P < 0.05) between the processes of salt. However both forms can be used for the development of technological processes of low investment by encouraging new options for fish consumption. O objetivo do presente estudo foi avaliar o processo da salga seca e salga úmida em filés de pacu (P. mesopotamicus), provenientes do cultivo em tanques-rede. Os filés com pele foram salgados na propor o de 40% de sal em rela o ao peso inicial, por um período de 10 dias. Após processamento, foram avaliados os parametros microbiológicos e de composi o centesimal dos filés submetidos a salga, bem como a determina o da concentra o de cloreto de sódio (NaCl) e cálculo de rendimento. Quanto à composi o centesimal foram avaliados os teores de proteína, extrato etéreo, umidade e matéria mineral da matéria-prima in natura e nos produtos processados por salga. As análises microbiológicas compreenderam a determina o de Coliformes a 45°C, Salmonella sp e Staphylococcus coagulase positiva. Os pacus submetidos a salga úmida e seca mantiveram-se dentro dos padr es microbiológicos estabelecidos pela legisla o. Os teores de umidade, proteína e lipídios e cloretos apresentaram diferen as (P < 0,05) entre os processos de salga. No entanto, ambas as formas podem ser utilizadas para o desenvolvimento de processos tecnológicos de baixo investimento incentivando novas op es para o consumo de pescado.
Proximate composition, microbiological and sensory of jundiá (Rhamdia quelen) submitted to the smoking process/ Composi o centesimal, microbiológica e sensorial do jundiá (Rhamdia quelen) submetido ao processo de defuma o
Cleiton Manske,Márcia Luzia Ferrarezi Maluf,Bruno Estev?o de Souza,Arcangelo Augusto Signor
Semina : Ciências Agrárias , 2011,
Abstract: The objective of the experiment was to evaluate the proximate composition, microbiological and sensory of jundia (Rhamdia quelen) of whole eviscerated and fillet submitted to the process of hot smoking. Were used 22 jundias, separated into three categories: A: small whole fish (122.25 ± 25.39g); B: headless large whole fish (358.4 ± 50.98g) and C: fillet (126.92 ± 12.48g). Samples of each category were used for microbiological analysis of raw material in natura. The remainder was submitted to dry salting with 25% of concentration for 60 minutes, after were realized the process of hot smoking. Wasn’t observed significative differences (P ? 0.05) between the parameters of crude protein, ether extract and moisture of smoked products. With the sensory analysis, was observed that the fillet had higher intensity of salt, differing (P ? 0.05) from the whole smoked fish. There were no significative differences (P ? 0.05) on the color and acceptance of products smoked. With the flavor, the tasters revealed preference (P ? 00.5) to small whole smoked fish. The result of the count of microorganisms was low, indicating that the products were under the standards established by law for consumption. It is concluded that the hot smoking process applied in different ways to the fish (small whole fish, headless large whole fish and fillet) does not affect the proximate composition of the final product. There was a significant preference for small whole fish smoked in the case of flavors and for headless large whole fish smoked in the case of acceptance, smoked jundia can being consumed with security for a period of 21 days of storage at temperature of ± 5oC beyond to add value to smoked fish. O objetivo do presente trabalho foi avaliar a composi o centesimal, microbiológica, e sensorial do jundiá (Rhamdia quelen) inteiro eviscerado e filetado submetido ao processo de defuma o a quente. Foram utilizados 22 jundiás, separados em três categorias: A: peixe inteiro pequeno eviscerado (122,25 ± 25,39g); B: peixe inteiro grande eviscerado e descabe ado (358,4 ± 50,98g) e C: filé (126,92 ± 12,48g). Amostras de cada categoria foram utilizadas para análise microbiológica da matéria prima in natura. O restante foi submetido a salga úmida na concentra o de 25% de sal por 60 minutos, posteriormente foi realizado o processo de defuma o a quente. N o foram observadas diferen as (P ? 0,05) entre os parametros de proteína bruta, extrato etéreo e umidade dos produtos defumados. Quanto à analise sensorial, foi observado que o filé apresentou maior intensidade de sal, diferindo (P ? 0,05
Densidade de estocagem e parametros eritrocitários de pacus criados em tanques-rede
Bittencourt, Fábio;Feiden, Aldi;Signor, Arcangelo Augusto;Boscolo, Wilson Rogério;Lorenz, Evandro Kleber;Maluf, Márcia Luzia Ferrarezi;
Revista Brasileira de Zootecnia , 2010, DOI: 10.1590/S1516-35982010001100002
Abstract: the aim of this study was to evaluate management, erythrocyte features and blood glucose level of pacu (piaractus mesopotamicus), stocked at different densities in cages. it was used 2,700 fish with initial average weight of142.11 ± 10.54 g distributed in nine cages of 5 m3 of useful volume during 240 days. a complete random design was used, with three densities (200, 300 and 400 fish per cage), each one with three replicates. only final weight and weight gain decreased with the increase of stocking density. final biomass increase according to the number of animals stocked per productive unit. densities of farming did not influence carcass yield, but they affected total length, which was higher in fish raised in low density, which did not differ from those raised in intermediate density. fish raised in lower density showed the greatest lipid deposition, but they did not significantly differ from those stocked in intermediate density. erythrocyte variables like total erythrocytes, hemoglobin, hematocrit, average corpuscular volume, average corpuscular hemoglobin concentrations and average corpuscular hemoglobin did not present statistical differences among the used densities and they remain in the normal range for the species. levels of glucose in the blood did not differ significantly among the analyzed densities. stocking densities do not influence erythrocyte parameters, glucose level neither carcass yields of pacu fish. density 200 fish/cage results in higher final weight and weight gain; however, it reduces productivity per area and increases lipid deposition of the fish.
Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova
Takahachi, Gisele;Maluf, Márcia Luzia Ferrarezi;Svidzinski, Terezinha Inez Estivalet;Akimoto-Günther, Luciene Setsuko;Hübler, Márcia Rosangela Neves de Oliveira;Bersani-Amado, Ciomar Aparecida;Cuman, Roberto Kenji Nakamura;
Brazilian Archives of Biology and Technology , 2006, DOI: 10.1590/S1516-89132006000700006
Abstract: the objective of this work was to evaluate biochemical parameters in paracoccidioides brasiliensis infected mice and the effect of canova medicine on these parameters. mice infected with the isolate paracoccidioides brasiliensis pb18 and treated with canova for 17 weeks were used. the biochemical parameters analysed were the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and amylase, and the concentration of total proteins, albumin and globulins. the results suggested that the animals that were treated with canova had less functional alterations in their internal organs.
Caracteriza??o de leveduras isoladas da vagina e sua associa??o com candidíase vulvovaginal em duas cidades do sul do Brasil
Ferrazza, Magda Helena S. H.;Maluf, Márcia L. Ferrarezi;Consolaro, Marcia Edilaine Lopes;Shinobu, Cristiane Suemi;Svidzinski, Terezinha Inez Estivalet;Batista, Márcia Regina;
Revista Brasileira de Ginecologia e Obstetrícia , 2005, DOI: 10.1590/S0100-72032005000200003
Abstract: purpose: to evaluate the distribution of yeast species isolated from the vagina in two cities of the south of brazil and compare the in vitro susceptibility profile of these yeasts against some antifungals, which are used in clinical routine. methods: all women attended from january to june 2004 for vaginal routine examinations, independent of being symptomatic or not were included in the study. only those who presented immunodeficiency like aids or any other genital infection were excluded. samples of vaginal discharge from the women (jaraguá do sul - sc (n=130) and maringá - pr (n=97)) were cultivated. the yeasts were identified and submitted to the susceptibility test against the antifungals fluconazole, nystatin and amphotericin b. results: the frequency of positive cultures for yeasts was the same in both cities; c. albicans was the most prevalent species (about 24%), but its frequency was different: in sc it corresponded to 77.4% of the yeasts both in symptomatic and asymptomatic women and in pr it was 50.0% with predominance in symptomatic women. we observed high rates of susceptibility to fluconazole and amphotericin b, but 51.1% of the yeasts presented dose-dependent susceptibility (dds) to nystatin. c. albicans showed a higher tendency to be nystatin resistant (52.8% dds) than non-albicans species (44.4%). conclusions: our data showed geographic differences among the species of yeasts isolated from the vagina and suggest that this fact has clinical relevance considering the differences in susceptibility, especially regarding nystatin, which could be important for the management of vulvovaginal candidiasis.
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