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Search Results: 1 - 10 of 401366 matches for " M Nateghi Rostami "
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Flow Cytometric Analysis of Leishmania Reactive CD4+/CD8+ Lymphocyte Proliferation in Cutaneous Leishmaniasis
M Nateghi Rostami,A Khamesipour,SE Eskandari,A Miramin Mohammadi
Iranian Journal of Parasitology , 2008,
Abstract: Background: Determination of the division history of T cells in vitro is helpful in the study of effector mechanisms against infections. Technique described here uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor the proliferation. Methods: In a cross sectional study, blood samples were collected from 7 volunteers with history of cutaneous leishmania-sis (CL) and one healthy control from endemic areas in Isfahan province who referred to the Center for Research and Training in Skin Diseases and Leprosy (CRTSDL), then CD4+/CD8+ lymphocytes and CD14+ monocytes were isolated from peri-pheral blood mononuclear cells (PBMC) using mAbs and magnetic nanoparticles. CFSE labeled CD4+ or CD8+ lympho-cytes cultured with autologous monocytes in the presence of PHA, SLA, live Leishmania major or as control with-out sti-mulation. Cells were harvested after 7 days and were analyzed using flow cytometry. Results: Five consecutive divisions were monitored separately. Stimulation of CD4+ or CD8+ lymphocytes from CL sub-jects with SLA showed a significant difference in proliferation comparing with unstimulated cells (P< 0.05). The signifi-cant difference in the percentages of CD4+ cells stimulated with SLA was revealed at different divisions for each subject. In CD8+ lymphocyte, significant stronger stimulation of SLA was evident later in the proliferation process. The mean number of divisions in both CD4+/CD8+ lymphocytes stimulated with SLA was significantly greater than when stimulated with live L. major (P=0.007 / P=0.012, respectively) Conclusion: The percentage of divided cells might be calculated separately in each division. The cells remained active following CFSE staining and there is possibility of functional analysis simultaneously.
Intestinal Parasitic Infections in Renal Transplant Recipients
M Nateghi Rostami,H Keshavarz,E Eskandari,EB Kia
Iranian Journal of Parasitology , 2007,
Abstract: Background: Organ transplant recipients can experience serious diseases from infections due to emerging and reemerging parasitic infections. This study was carried out to evaluate the prevalence of intestinal parasites among renal transplant re-cipients of Iran. "nMethods: This cross-sectional study was conducted from June 2003 to August 2004 on renal transplant recipients in Iran. A total of 706 fecal samples obtained from randomly selected population originated from all over Iran. Patient's information was recorded in a questionnaire before sampling. A sample of stool was taken from each person. Direct wet smear exami-nation, formalin-ether concentration, Ziehl-neelsen staining, and agar plate culture were done for each sample. "nResults: Totally 32 patients (4.5%) were positive for parasitic infections. In searching for emerging parasitic infections, the most prevalent parasites were found to be Blastocystis hominis, Giardia lamblia and Entamoeba coli, respectively. The merely ova which were seen were related to Hymenolepis nana. With investigation of healthy control, no significant differ-ence was found between transplanted and normal population. "nConclusion: The population showed controlled rate of intestinal infections probably due to regular awareness concerning risks of opportunistic infections; albeit regular surveillance through routine examination of stool samples for parasites seems considerably advantages the transplant recipient patients.
Serological Study of Toxoplasma gondii Infection Using IFA Method in Renal Transplant Recipients
M Nateghi Rostami,E Eskandari,Z Garoosi,N Mohajeri
Iranian Journal of Parasitology , 2006,
Abstract: Toxoplasmosis is a wide distributed opportunistic infection caused by Toxoplasma gondii. This was a cross-sectional study of T. gondii antibody titer, which was conducted from June 2003 to August 2004 on renal transplant recipients in Iran. A total of 551 serum samples were obtained from randomly selected population referred from different areas all over the country to Shafa Central Clinic in Tehran. Patient’s information was recorded in a questionnaire before sampling. Two samples of finger-prick blood were collected from each person and antibody titer against Toxoplasma was assessed by Indirect Fluorescence Antibody (IFA) technique on serum samples. Totally 39 cases (7.1%) of samples were positive for antibody by the titer of 1: 20 and higher. On investigation of risk factors, no significant difference was found between consumption of under-cooked meat, close contact with animals, and the source of drinking water and seropositivity rate of toxoplasmosis. The relatively low seroprevalence rate of Toxoplasma infection shows the successful approaches to awareness of transplant recipients about the potential risks of acquisition of infectious diseases due to regular administration of suppressive drugs. However, the regular surveillance through serological screening of Toxoplasma antibody in kidney transplant recipients is advisable.
Phenotyping of peripheral memory T cell subsets in cutaneous leishmaniasis
Ali Khamesipour,Mahmoud Nateghi Rostami,Hossein Keshavarz,Akram Miramin Mohammadi
Dermatology and Cosmetic , 2010,
Abstract: "nBackground and Aim: The heterogenous population of memory T lymphocytes is distinguished based on surface markers and effector functions such as cytokine secretion. Recently, two subsets of memory T cells are defined by expression of chemokine receptor CCR7 and CD45RA designating as "central memory" T cells (TCM) and "effector memory" T cells (TEM). The objective of this staudy was to evaluate the phenotype and function of these lymphocytes in healed cases of cutaneous leishmaniasis."n"nMethods: The phenotype of lymphocytes were determined in blood samples of 13 volunteers with history of self healing cutaneous leishmaniasis (HCL) and in 6 healthy controls."n"nResults: No significant difference was found in memory T cell subsets between HCL volunteers and healthy controls using flow cytometry. However, following sorting of different memory subsets, a significantly higher proliferation was seen in cells of HCL volunteers comparing to the control group. A significantly higher IFN-γ response in TEM and a significantly higher IL-2 response in TCM were observed in cell culture of HCL volunteers comparing controls."n"nConclusion: The responses were elicited when the cells were stimulate with SLA in vitro, it is concluded Leishmania-specific TEM and Leishmania-specific TCM subsets exist in HCL volunteers and since the volunteers with history of CL presumed to be protected against reinfection, it seems that both TCM and TEM play role in the protection against Leishmania infection in these individuals.
CD8+ T Cells as a Source of IFN-γ Production in Human Cutaneous Leishmaniasis
Mahmoud Nateghi Rostami,Hossein Keshavarz,Rosita Edalat,Abdolfattah Sarrafnejad,Tahereh Shahrestani,Fereidoun Mahboudi,Ali Khamesipour
PLOS Neglected Tropical Diseases , 2010, DOI: 10.1371/journal.pntd.0000845
Abstract: Background In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4+/CD8+ T cells in response to Leishmania antigens were assessed at transcript and protein levels in 14 volunteers with a history of self-healing cutaneous leishmaniasis (HCL) and compared with 18 healthy control volunteers. Methodology/Principal Findings CD4+/CD8+/CD14+ cells were purified from peripheral blood using magnetic beads; CD4+/CD8+ T cells were co-cultured with autologous CD14+ monocytes in the presence of soluble Leishmania antigens (SLA). Stimulation of either CD4+ T cells or CD8+ T cells of HCL volunteers with SLA induced a significantly (P<0.05) higher IFN-γ production compared with the cells of controls. Upregulation of IFN-γ gene expression in CD4+ cells (P<0.001) and CD8+ cells (P = 0.006) of HCL volunteers was significantly more than that of controls. Significantly (P<0.05) higher fold-expression of IFN-γ gene was seen in CD4+ cells than in CD8+ cells. In HCL volunteers a significantly (P = 0.014) higher number of CD4+ cells were positive for intracellular IFN-γ production than CD8+ cells. Conclusions/Significance Collectively, the volunteers have shown maintenance of specific long-term immune responses characterized by a strong reaction to leishmanin skin test and IFN-γ production. The dominant IFN-γ response was the result of expansion of both CD4+ and CD8+ T cells. The results suggested that immune response in protected individuals with a history of zoonotic cutaneous leishmaniasis (ZCL) due to L. major is mediated not only through the expansion of antigen-specific IFN-γ producing CD4+ Th1 cells, but also through IFN-γ producing CD8+ T cells.
Transient Stability Analysis of Power System by Coordinated PSS-AVR Design Based on PSO Technique  [PDF]
A. D. Falehi, M. Rostami, Hassan Mehrjadi
Engineering (ENG) , 2011, DOI: 10.4236/eng.2011.35055
Abstract: In this paper, Power System Stabilizer (PSS) and Automatic Voltage Regulator (AVR) are coordinated to improve the transient stability of generator in power system. Coordinated design problem of AVR and PSS is formulated as an optimization problem. Particle Swarm Optimization (PSO) technique is an advanced robust search method by the swarming or cooperative behavior of biological populations mechanism. The performance of PSO has been certified in solution of highly non-linear objectives. Thus, PSO technique has been employed to optimize the parameters of PSS and AVR in order to reduce the power system oscillations during the load changing conditions in single-machine, infinite-bus power system. The results of nonlinear simulation suggest that, by coordinated design of AVR and PSS based on PSO technique power system oscillations are exceptionally damped. Correspondingly, it’s shown that power system stability is superiorly enhanced than the uncoordinated designed of the PSS and the AVR controllers.
The role of sr and nd isotopes in modelling the magmatic processes producing mesozoic granitoids in the north american western cordillera near the USA-Canada boundary
V. E. Vernon-Chamberlain,M. Nateghi
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02891611
Factors affecting results of lymph node dissection in patients with skin Melanoma, 1987-92
Mir M,Nateghi F,Zeraaty H
Tehran University Medical Journal , 1998,
Abstract: In this paper, factors affecting the result of prophylactic lymph node dissection in 125 patients with skin melanoma during the period between 1987-1992 in the cancer institute were evaluated and prognostic parameters ascertained. The result showed prevalence of head and neck melanoma in men were two times more than in women with a preponderance of regional and lymphatic recurrence. Majority of patients presented with advanced clark levels (76.8 Pct). Clinical and histologic evaluation of the regional lymph nodes failed to show any meaningful relation.
The Evaluation of Serum Nitrite, Nitrate and Malonyldialdehyde Levels in Smokers
Rostami, M,Jorfi, M
Medical Laboratory Journal , 2010, DOI: http://www.goums.ac.ir/mljgoums/index.php?slc_lang=en&sid=1
Abstract: Background and objectives: Smokers are exposed to significantquantities of ROS (Reactive Oxygen Species); and The Level of NitricOxide (NO), the primary vasodilator produced by endothelial cells, ischanged by cigarette smoking. Cigarette smoking is associated withimpaired endothelium-dependent vasodilatation and cardiovasculardisease (CVD). The aim of this study is to determine the level of serumnitrite, nitrate and malondialdehyde (MDA) levels in smokers.Material and Methods: In this descriptive analytical study, 60 healthymale smokers and 60 male non-smokers (control group) were selected bya Purposive sampling and then serum levels of nitrite, nitrate and MDAin all patients were determined and compared to together.Results: Serum nitrite and nitrate level in smokers are 10.4±3.1 and19.6±5.9 and in non-smokers is 14.6±4.4 and 29.3±6.7 (p<0.00001)μmol/L, respectively. The results show that smokers’ are significantlylower than non-smokers’. Serum MDA level in smokers (11.7±2.6μmol/L) is significantly higher (p<0.00001) than non-smokers (8.3±1.9μmol/L).Conclusion: Based on the results, serum level of nitrite and nitrate arelower and MDA is higher in smokers. This difference can be related toCVD in smokers.Keywords: Smokers, Non-smokers, Nitrite, Nitrate, Malodialdehyde(MDA), cardiovascular disease (CVD).
Tuberculin test in nursing and human-sciences students
M Golchin,M Rostami
Journal of Research in Medical Sciences , 2005,
Abstract: Introduction: Tuberculosis (TB) is a leading cause of death worldwide due to any single infectious agent. It seems that health care workers including nursing students can be affected easier than the other people, because of probable contacts in hospital. The risk of TB infection in nursing students has not estimated in Iran, so we conducted this study to compare the results of tuberculin test in the beginning and the end of educational course for nursing and human-sciences student. Methods: In a Cohort study, 320 students (160 nursing and 160 human-sciences) underwent PPD skin test (5 units RT 23) at the beginning and the end of educational course by expert technician. The data of remaining students (123 nursing and 111 human-sciences) were analyzed by SPSS software using Wilcoxson and Mann-Whitney tests. Results: The frequency distribution of skin reaction in nursing students was negative (0-4 mm: 93.7%), suspected (5-9 mm: 4.4%) and significant (≥15mm: 1.9%) at the beginning of study, while it was negative( 75.5 %), suspected (9.8%), positive (10-14 mm 3.3%) and significant (11.4%) at the end of study. The frequency of skin reaction in human-sciences student was negative (93.7%), suspected (0.6%), positive (1.3%) and significant (4.4%) at the beginning of study, while it was negative (79.3%), significant (10.8%), suspected (8.1%) and positive (1.8%) at the end of study. The difference in that proportion of nursing students and control group with positive and significant PPD test at the end of study was statistically significant. The difference for the above proportions between two groups was not statistically significant. Conclusion: All subjects had no significant difference regarding to age, indigenous area and PPD test. Both groups have the same chance for exposure to M. Tuberculosis. The rate of new TB infection in Iranian community has diminished in comparison with the last few decades. Although risk of new infection may be a little bit more after age of 18, but it is still much lower than countries of high burden. The epidemiologic pattern of TB transmission has changed and it is going to become similar to that of developed countries. Key words: Tuberculosis, PPD skin test, nursing students
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