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Search Results: 1 - 10 of 1852 matches for " Luciane Dall'Agnol "
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Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology
Roberta Bussamara,Luciane Dall'Agnol,Augusto Schrank,Kátia Flávia Fernandes,Marilene Henning Vainstein
Enzyme Research , 2012, DOI: 10.1155/2012/329178
Abstract: This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150?min incubation time, pH 4.76, and an enzyme/support ratio of 1282?U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68°C and 52°C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone. 1. Introduction Biocatalyst enzymes play an important role in biotechnological applications due to their extreme versatility with respect to substrate specificity and stereoselectivity and exhibit many other features that render their use advantageous when compared to conventional chemical catalysts. As an example, fat and oil hydrolysis using NaOH as a catalyst requires high pressure and temperature to achieve high efficiency (97-98%); in contrast, the same process can be carried out effectively at normal temperature and pressure using lipases, with significant decrease in wastewater production. Lipases (triacylglycerol acylhydrolase; EC catalyze the hydrolysis of triglycerides to glycerol and fatty acids, as well as a variety of reactions in nonaqueous medium (e.g., transesterification, esterification, and interesterification). Such enzymatic properties allow a series of biotransformation reactions that lead to multiple industrial applications in foods, flavors, pharmaceuticals, detergent formulation, oil/fat degradation, cosmetics, and environmental remediation [1–4]. However, soluble enzymes usually exhibit lower stability than chemical catalysts and often cannot be recovered and reused. This severely hinders their application in practice. Nevertheless, this problem can be overcome by enzyme immobilization, which enhances thermal and operational stabilities, ease of handling, and prevention of aggregation and autolysis. Besides, immobilized lipases (IE) on solid support allow recoverability and reuse thus significantly
Validation of a Brazilian quantitative sensory testing (QST) device for the diagnosis of small fiber neuropathies
Schestatsky, Pedro;Stefani, Luciana Cadore;Sanches, Paulo Roberto;Silva Júnior, Danton Pereira da;Torres, Iraci Lucena Silva;Dall-Agnol, Letizzia;Balbinot, Luciane Fachin;Caumo, Wolnei;
Arquivos de Neuro-Psiquiatria , 2011, DOI: 10.1590/S0004-282X2011000700019
Abstract: quantitative sensory testing (qst) is defined as the determination of thresholds for sensory perception under controlled stimulus. our aim was to validate a new qst device for brazilian sample. in 20 healthy adults, thermoalgesic thresholds were assessed using a qst prototype (heat pain stimulator-1.1.10; brazil). a 30 × 30 mm2 thermode with a 1°c/s stimulus change rate were applied. thresholds of three consecutive stimuli were averaged in two different sessions separated by at least two weeks. additionally long thermal heat pain stimulus was performed. to evaluate the consistency of our method we also analyzed 11 patients with small fiber neuropathy. results showed good reproducibility of thermal perception thresholds in normal individuals and plausible abnormal thresholds in patients. we conclude that our qst device is reliable when analyzing the nociceptive pathway in controls and patients.
O igualitarismo liberal de Dworkin
Dall'Agnol, Darlei;
Kriterion: Revista de Filosofia , 2005, DOI: 10.1590/S0100-512X2005000100005
Abstract: this paper sorts out the main elements in dworkin's political philosophy here called "liberal igualitarianism." to reach this aim, it reconstructs his conception of law and his understanding of the citizens' most fundamental right to equal respect and concern. moreover, it analyzes his attempt to reconcile this formal equality, as well as a more substantial equality, with freedom
Wave propagation in a non-uniform string
Dall'Agnol, Fernando Fuzinatto;
Revista Brasileira de Ensino de Física , 2011, DOI: 10.1590/S1806-11172011000400006
Abstract: in this article i present the behavior of the reflection and transmission coefficients of a pulse at a joint between two strings with mass densities μ1and μ2. the joint is made of a string segment with mass density varying linearly from μ1 to μ2. it will be shown that the reflection of the pulse at the joint depends largely on the ratio between the pulse width and the length of the joint. analogies with other physical systems such as antireflection coatings and tsunamis will be considered briefly.
Pressupostos metaéticos e normativos para uma nova ética Ambiental
Darlei DallAgnol
Princípios : Revista de Filosofia , 2007,
Abstract: This paper deals with the meta-ethical and normative assumptions for a new environmental ethics. Particularly, it tries to show that the realistic meta-ethics about intrinsic value underlying many of the writings of the so-called “deep ecology” is flawed. It presents, then, a different conceptual analysis of intrinsic value and sorts out its normative and practical implications for an ethics concerned with the environment.
Review: Moral knowledge? New readings in moral epistemology
Darlei Dall'Agnol
Principia : an International Journal of Epistemology , 2000,
Abstract: Review
Quine and Wittgenstein: The End of Analytic Philosophy?
Darlei Dall'Agnol
Principia : an International Journal of Epistemology , 2003,
Abstract: This paper deals with the question whether science and philosophy are continuous, as Quine thought, or whether they are completely separated, as Wittgenstein held. Reconstructing the reasons why the latter kept a sharp distinction between science and philosophy, it examines the attempts of the former to resolve philosophical problems in scientific terms. It maintains that Quine’s scientism is misconceived and presents further reasons for making a distinction (if not a separation) between science and philosophy.
O PPGENF-UFRGS no compasso dos avan os da pós-gradua o em enfermagem El PPGENF-UFRGS al compás de los avances del postgrado en enfermería PPGENF-UFRGS and the advances of graduate studies in nursing
Clarice Maria Dall'agnol
Revista Gaúcha de Enfermagem , 2010, DOI: 10.1590/s1983-14472010000300001
Abnormal Returns and Contrarian Strategies
Marco Bonomo,Ivana Dall'Agnol
Revista Brasileira de Finan?as , 2003,
Abstract: We test the hypothesis that strategies which are long on portfolios of looser stocks and short on portfolios of winner stocks generate abnormal returns in Brazil. This type of evidence for the US stock market was interpreted by The Bondt and Thaler (1985) as reflecting systematic evaluation mistakes caused by investors overreaction to news related to the firm performance. We found evidence of contrarian strategies profitability for horizons from 3 months to 3 years in a sample of stock returns from BOVESPA and SOMA from 1986 to 2000. The strategies are more profitable for shorter horizons. Therefore, there was no trace of the momentum effect found by Jagadeesh and Titman (1993) for the same horizons with US data. There are remaing unexplained positive returns for contrarian strategies after accounting for risk, size, and liquidity. We also found that the strategy profitability is reduced after the Real Plan, which suggests that the Brazilian stock market became more efficient after inflation stabilization.
Privacidade dos pacientes: uma quest?o ética para a gerência do cuidado em enfermagem
Soares, Narciso Vieira;Dall'Agnol, Clarice Maria;
Acta Paulista de Enfermagem , 2011, DOI: 10.1590/S0103-21002011000500014
Abstract: objective: to identify patient perceptions in an inpatient unit with respect to issues related to privacy in the hospital. methods: this was a qualitative, exploratory, descriptive study. the sample consisted of 12 patients admitted to a general hospital; data collection occurred through semi-structured interviews and simple observation of the ambience of this setting. results: everyday situations showed the occurrence of violation of personal space and the patient's body, sometimes without apparent reason. the experience of exposure of one's body and the inadequate professional posture of the nursing staff, the vision of patients was formed under conditions that generated anxiety, embarrassment and stress that impacted on their health and well-being. conclusion: the statements made clear the low expectation of patients about privacy in the care received at the institution, with privacy related to technical competence and knowledge of professionals about the procedures they perform, such as linking ideas including helpfulness and kindness with their treatment.
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