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Search Results: 1 - 10 of 21066 matches for " Luciana Cordeiro do Nascimento "
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Avalia??o da atividade de indutores de resistência abiótica, fungicida químico e extratos vegetais no controle da podrid?o-negra em Abacaxi 'Pérola'
Oliveira, M?nica Danielly de Mello;Nascimento, Luciana Cordeiro do;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000100013
Abstract: black rot of pineapple, caused by chalara paradoxa (de seyn.) sacc., is a postharvest disease responsible by high losses on fruits destined to the fresh market and to the processing industry. penetration of fungus inside cells occurs through wounds and stem cutting, causing infection. the objective of this work was to evaluate the effect of abiotic resistance inducers, chemical fungicide and natural plant extracts on black rot of pineapple control. 32 fruits of pineapple cv pérola were used. they were disinfested with sodium hypochlorite (commercial product) at 4% for 5 minutes. after drying at room temperature, fruits were treated, by spraying, with: 1) distilled water (control), 2) derosal 3) bion? (acibenzolar-s-methyl); 4) ecolife?; 5) agro-mos?; 6) allium sativum extract at 20%; 7) a. cepa at 20% and 8) azadirachta indica at 20%. treated fruits were incubated on humid chamber with polyethylene bags during 24 hours before inoculation procedure using a mycelia disk added to a wound at the epidermic area of the fruit. evaluation of disease progress was done by a disease index: 1- no symptoms, 2- black rot on epidermis reaching 1-5 simple fruits, 3- black rot on epidermis reaching 6-10 simple fruits, 4- internal brown yellow rot, 5- black rot and disintegration of internal area in more than 50%. the experimental design was a completely randomized with eight treatments and five replicates, using general linear models with multinomial distribution and the averages were compared by scott-knott test at 5%. the best results were found in the ecolife treatment with longer fruit life span and less severity in the symptoms of the disease.
Termoterapia para o controle de patógenos em pós-colheita em frutos da cajazeira = Thermotherapy for post harvest pathogens on Spondias fruits
Carlos Henrique de Brito,Nivania Pereira da Costa,Jacinto de Luna Batista,Luciana Cordeiro do Nascimento
Acta Scientiarum : Agronomy , 2008,
Abstract: O tratamento térmico, principalmente água quente, é método alternativo que tem sido utilizado para o controle de doen as e infesta es de insetos em frutos póscolheita. O presente trabalho teve como objetivo determinar a combina o de tempo e temperatura adequada para o controle de fungos de pós-colheita em frutos de cajazeira em atmosfera ambiente. No primeiro tratamento, os frutos foram imersos em água quente e no segundo foram expostos ao vapor a 50°C por 0, 10, 20, 30 e 40 minutos para diferentes lotes de frutos. Foram retiradas de cada fruto/tratamento quatro sec es, as quais foram incubadas em placas de Petri com BDA, sendo realizadas as avalia es da incidência de fitopatógenos após 7 dias de incuba o. Os resultados obtidos demonstraram uma maior incidência de Rhizophus sp. nos tratamentos avaliados e redu o de Aspergillus sp. e Fusariumsp., cujo comportamento foi influenciado pelo tratamento termoterápico, podendo ser indicado os tratamentos vapor e banho-maria a 50oC a partir de 20 minutos como método alternativo no controle pós-colheita de Aspergillus sp. e Fusarium sp. em frutos da cajazeira. Thermal treatment, mainly hot water, is an alternative method that has been used for diseases and pests infestation in post harvest fruits. The present work aimed to determine a combination of correct time x temperature for post harvest fungus control on Spondias fruits. For the first treatment, fruits were dipped on hot water and, for the second, on hot air, both with 50°C for 0, 10, 20, 30 e 40 minutes for different fruit groups. Four pieces were sectioned from each fruit, per treatment, and incubated in Petri dishes with BDA,being evaluated for fungus incidence after seven days incubation. Obtained results showed higher incidence of Rhizopus sp. on the evaluated treatments, and a reduction of Aspergillus sp. and Fusarium sp., while behaviour was influenced by thermotherapy, indicating air and hot water at 50oC, for 20 min., as an alternative method for post harvest disease control caused by Aspergillus sp. and Fusarium sp. on Spondias fruits.
Controle de Colletotrichum gloeosporioides em mamoeiro, utilizando extratos vegetais, indutores de resistência e fungicida = Alternative control of Colletotrichum gloeosporioides in papaya, using vegetable extracts, resistance inducers and fungicide
Luciana Cordeiro do Nascimento,Aparecida Rodrigues Nery,Luís Nery Rodrigues
Acta Scientiarum : Agronomy , 2008,
Abstract: O presente trabalho teve como objetivo avaliar o controle de doen as do mamoeiro, utilizando extratos vegetais (alho, angico e manjeric o), óleos naturais (urucum e algod o), indutores de resistência (Bion , Ecolife e Agro-mos ) e fungicida Mancozeb/Dithane. O Experimento I consistiu-se da avalia o dos tratamentos sobre o crescimento micelial de Colletotrichum gloeosporioides, in vitro; no Experimento II, efetuou-se ainocula o artificial do fungo sobre frutos sadios de mam o e avaliou-se a severidade da podrid o peduncular, por meio de escala de notas e, no Experimento III, aplicaram-se os tratamentos em mudas de mamoeiro, inoculadas artificialmente com C. gloeosporioides,avaliando-se a antracnose com escala de notas. Os resultados obtidos demonstraram que extratos de angico e alho proporcionaram um menor crescimento micelial, in vitro, e o Bion manteve um eficiente controle da podrid o peduncular em frutos. Nas mudas, oefeito do alho, angico e Bion diferiram significativamente dos demais, sendo que o Bion apresentou melhor controle da severidade da antracnose. Assim, o controle da podrid o peduncular e antracnose em mamoeiro, com produtos naturais e indutores de resistência, pode ser uma op o viável na substitui o de agroquímicos. This present work had as objective to evaluate the control of papaya diseases using natural extracts (Allium sativum L., Anadenanthera peregrina L. Speng, and Ocimum basilicum L.), natural oils (Gossypium hirsutum L. and Bixa orellana L.), resistance inducers (Bion , Ecolife and Agro-mos ), and a chemical fungicide (Mancozeb/Dithane). Experiment I was conducted to evaluate the inhibitory potential of the treatments on Colletotrichum gloeosporioides mycelial growth in vitro; in Experiment II, artificial inoculation of fungus was performed on healthy papaya fruits, and the severity of stem-end rot was evaluated using a disease index; and in Experiment III,treatments were applied on papaya seedlings artificially inoculated with C. gloeosporioides, and anthracnose was evaluated by means of a disease index. Obtained results showed that extracts of Anadenanthera and Allium were responsible for lower mycelial growth in vitro, andBion was efficient for papaya stem-end rot. In papaya seedlings, the effects of garlic, angico and Bion differed significantly from the rest, with Bion showing the best results for anthracnose control. Papaya seeds were not affected by C. gloeosporioides. Thus, alternative control to stem-end rot and anthracnose on papaya with natural products and resistance inducers should be an option viable fo
Qualidade sanitária e fisiológica de sementes de Amburana cearensis A.C. Smith submetidas à termoterapia e tratamento químico = Health and physiological quality of Amburana cearensis A.C. Smith seeds subjected to thermotherapy and chemical treatment
M?nica Danielly de Mello Oliveira,Luciana Cordeiro do Nascimento,Edna Ursulino Alves,Edilma Pereira Gon?alves
Acta Scientiarum : Agronomy , 2011,
Abstract: O objetivo deste trabalho foi avaliar a eficácia da termoterapia no controle de fungos associados a sementes de cumaru (Amburana cearensis A.C. Smith) de duas localidades, após tratamento térmico e químico. A avalia o da sanidade foi realizada pelo método de incuba o em papel filtro (Blotter Test) e a qualidade fisiológica das sementes pelos testes de germina o e condutividade elétrica. O tratamento térmico utilizado foi por imers o em água aquecida a 60°C por 0, 5, 10 e 20 min. e o tratamento químico foi feito com o fungicida Captan. Utilizaram-se 100 sementes por tratamento (dez sementes por placa de Petri), as quais foram incubadas, por sete dias, à temperatura de 22 ± 2oC e fotoperíodo de 12h. A avalia o qualitativa e quantitativa (expressa em porcentagem) dos fungos associados às sementes foi realizada com auxílio de microscópio estereoscópico. Nas amostras examinadas, constatou-se apenas a incidência do fungo Aspergillus niger. Houve maior incidência de A. niger no lote da localidade Catolé do Rocha, Estado da Paraíba, sendoa incidência do fungo muito baixa na localidade Petrolina, Estado do Pernambuco. Os tratamentos que apresentaram melhor resultado foram o fungicida e a termoterapia por 20 min. The objective of this work was to evaluate the effectiveness of thermotherapy in the control of fungi associated with seeds of cumaru (Amburana cearensis A.C. Smith) from two localities, after thermal andchemical treatment. The health assessment was conducted by the method of incubation on paper filter (Blotter Test) and physiological quality seeds was determined by germination tests and electrical conductivity. The thermal treatment was used by immersion in waterheated to 60 degrees for 5, 10 and 20 minutes. The chemical treatment was done with Captan fungicide. The study used 100 seeds per treatment (10 seeds per petri dish), which were incubated for seven days, at a temperature of 22 ± 2°C and 12-hour photoperiod. Thequalitative and quantitative assessment (expressed in percentage) of the fungi associated with the seeds was done with the help of a stereoscope. In the examined samples, only the Aspergillus niger fungus was found. There was a higher incidence of A. niger in the lot from the town of Catolé Rocha, Paraíba State, with very low incidence of the fungus in the town Petrolina, Pernambuco State. The treatments with the best results were fungicide and thermotherapy for 20 minutes.
Incidence of fungus and physiological quality of seeds of Jatropha curcas L. after cryogenic storage
Míriam Goldfarb,Maria Elita Martins Duarte,Mário Eduardo R. M. Cavalcanti Mata,Luciana Cordeiro do Nascimento
Biotemas , 2010,
Abstract: The objective of this work was to evaluate the incidence of fungi in stored seeds of Jatropha curcas. The research was carried out at Cryogenic/UFCG, Sanity/UFPB and Cotton/Embrapa. The material for analysis showed an 8% water level, and 200 seeds were stored for treatment in cryogenic containers with nitrogen in the vapor and liquid phases. Four periods of crioconservation (0, 30, 60 and 90 days), were employed. After each period, the seeds were tested for sanity (Blotter test) and germination. Superficial disinfestation, was carried out and seeds were distributed in Petri dishes, for incubation at 25 ± 2oC, over a period of 7 days. The evaluation of the incidence of fungi was carried out in a stereoscopic microscope with observation of fungal structures, and values were expressed as percentages of seeds with fungus. The statistical experiment was completely randomized with temperature x days of storage. Analysis of variance was conducted and the means were compared by Tukey’s test at 5%. After 30 days of cryogenic storage, a greater incidence of Aspergillus sp., Cladosporium sp. and Fusarium sp. was detected. It was concluded that crioconservation at cryogenic temperatures did not reduce the incidence of fungus on Jatropha curcas seeds. The physiological quality was preserved during the cryoconservation.
Qualidade sanitária e fisiológica de sementes de Amburana cearensis A.C. Smith submetidas à termoterapia e tratamento químico
Oliveira, M?nica Danielly de Mello;Nascimento, Luciana Cordeiro do;Alves, Edna Ursulino;Gon?alves, Edilma Pereira;Guedes, Roberta Sales;Silva Neto, Jo?o José da;
Acta Scientiarum. Agronomy , 2011, DOI: 10.4025/actasciagron.v33i1.5645
Abstract: the objective of this work was to evaluate the effectiveness of thermotherapy in the control of fungi associated with seeds of cumaru (amburana cearensis a.c. smith) from two localities, after thermal and chemical treatment. the health assessment was conducted by the method of incubation on paper filter (blotter test) and physiological quality seeds was determined by germination tests and electrical conductivity. the thermal treatment was used by immersion in water heated to 60 degrees for 5, 10 and 20 minutes. the chemical treatment was done with captan fungicide. the study used 100 seeds per treatment (10 seeds per petri dish), which were incubated for seven days, at a temperature of 22 ± 2oc and 12-hour photoperiod. the qualitative and quantitative assessment (expressed in percentage) of the fungi associated with the seeds was done with the help of a stereoscope. in the examined samples, only the aspergillus niger fungus was found. there was a higher incidence of a. niger in the lot from the town of catolé rocha, paraíba state, with very low incidence of the fungus in the town petrolina, pernambuco state. the treatments with the best results were fungicide and thermotherapy for 20 minutes.
Métodos de inocula??o de Phakopsora euvitis Ono em Vitis labrusca L.
Gomes, Erbs Cintra de Souza;Nascimento, Luciana Cordeiro do;Perez, Jane Oliveira;Leite, Rodrigo Pereira;Silva, Fábio Júnior Araújo;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000400037
Abstract: rust is one of the most important diseases in grapevine, with occurrence recorded in many production areas. in this research it was evaluated three methods of phakopsora euvitis inoculation on grapevine seedlings cv. 'isabel' with the objective of selecting an inoculation method for rapid pathogenicity determination. grapevine seedlings were inoculated with p. euvitis and maintained under conditions of 25±4c and ur 85±10% for 13 days. the experimental design was completely randomized, with 10 replications. the treatments were: t1 - brush of pathogen spores; t2 - spores suspension spraying (2.7x103 spores ml-1); t3 - disks of grapevine leaves with symptoms stuck in five leaves per plant and t4 - control. the spore brushing method was the most efficient, showing early symptoms at five days, and 100% of the leaves showing pathogen symptoms 13 days after treatment.
Efeito de extratos vegetais no controle de Fusarium oxysporum f. sp tracheiphilum em sementes de caupi
Silva, Jandiê Araújo da;Pegado, Claúdia Maria Alves;Ribeiro, Valéria Veras;Brito, Noelma Miranda de;Nascimento, Luciana Cordeiro do;
Ciência e Agrotecnologia , 2009, DOI: 10.1590/S1413-70542009000200039
Abstract: wilt caused by fusarium sp. in cowpea crop is a disease of great importance because it causes yield decrease. the control of this disease has been accomplished with uncontrolled applications of chemical fungicides causing problems for humans and environment. the present work had the objective to compare the effect of natural extracts on the control of fusarium oxysporum f. sp tracheiphlum, originated from seeds of cowpea (vigna unguiculata l), compared to chemical fungicide effect. extracts of allium sativum, anadenanthera colubrine, and ocimum basilicum were used alone or in combination with mancozeb. a 50 μl aliquot of each treatment was added in a hole (6 mm of diameter) made in the center of petri dishes with bda and a fungus mycelia disk was inoculated on it. evaluations were done during seven days, measuring the pathogen mycelial growth. seeds of cowpea were disinfected with sodium hypochlorite, sowed in plastic bags with autoclaved soil. eight days after germination, wounds were made on the basal part of the plantlets, and fungus spore suspension (1.4 x 10-5 con/ml) was applied on it. evaluations of the disease severity were accomplished daily during 30 days after inoculation, using a disease index. combination of extracts of a. sativum + ocimum basilicum and anadenanthera colubrina + ocimum basilicum did not reduce fungus mycelial growth, whereas ocimum basilicum extract alone showed minor mycelial growth, indicating fungicide action of this treatment on f. oxysporum f. sp tracheipphlum under the conditions studied. combination of mancozeb + anadenanthera colubrina was responsible for lower average of severity on the evaluated plants.
Evaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation
Nascimento, Luciana Cordeiro do;Pensuk, Viboon;Costa, Nivania Pereira da;Assis Filho, Francisco Miguel de;Pio-Ribeiro, Gilvan;Deom, Carl Michael;Sherwood, John;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006000600006
Abstract: the objective of this work was to evaluate the reactions of three peanut breeding lines (ic-10, ic-34, and icgv 86388) to tomato spotted wilt virus (tswv) by mechanical and thrips inoculation, under greenhouse conditions, and compare them to the reactions of cultivars sunoleic, georgia green, and the breeding line c11-2-39. tswv infection by mechanical inoculation was visually assessed using an index ranging from 0 (no symptoms) to 4 (apical death). enzyme-linked immunosorbent assay was used to confirm tswv infection from both mechanical and thrips inoculations. ic-10, ic-34, icgv 86388, and c11-2-39 were more resistant than the cultivars sunoleic and georgia green based on mechanical inoculation. upon thrips inoculation only ic-34 and icgv-86388 were infected by tswv, as demonstrated by reverse transcription polymerase chain reaction (rt-pcr), although no symptoms of infection were observed. the peanut breeding lines ic-10, ic-34, and icgv 86388 show higher level of resistance to tswv than cultivar georgia green considered a standard for tswv resistance.
Podrid?o peduncular e qualidade de mangas 'Tommy atkins' procedentes do mercado atacadista de Campina Grande-PB
Gomes, Erbs Cintra de Souza;Gondim, Perla Joana Souza;Santos, Mary de Fátima Guedes dos;Nascimento, Luciana Cordeiro do;Batista, Jacinto de Luna;Silva, Silvanda de Melo;
Revista Brasileira de Fruticultura , 2010, DOI: 10.1590/S0100-29452010005000133
Abstract: brazil stands out as one of the largest producers of mango (mangifera indica l.) in the world. however, significant losses are observed in all stages of production chain, mainly in the retail market, which are correlated almost entirety to incidence of fungal diseases. in this way, the objective of this study was to determine the incidence of natural infection of lasiodiplodia theobromae, causal agent of stem-end rot, and postharvest quality of 'tommy atkins' mangos, traded in the wholesale market of the central de abastecimento (empasa) from campina grande, paraíba state, brazil. to determine natural incidence of the disease it was collected 40 fruits at mature-green maturity stage from four different wholesale marketing stores at empasa that were sent to laboratory of phytopathology (cca/ufpb). after sanitization, fruits were kept at room conditions (28 ± 2 oc and rh 75 ± 6%) during eight days, determining first symptoms and signals of the pathogen. the characteristics of quality were determined by collecting 10 fruits from each wholesale marketing store. fruits were transported to the laboratory of biology and postharvest technology (cca/ufpb), where it was evaluated soluble solids (ss), titratable acidity (ta), ss/ta, and ph. data were subjected to variance analysis of and means compared by the tukey test at 5% of probability. mango commercialized in the wholesale market of empasa, campina grande, showed low ss and high ta contents characterizing fruit at the beginning of maturation. fruits showed 88.9% of natural incidence of stalk rot in the eighth day of storage, which is deleterious for retail quality of fruits, characterizing a causal factor for the higher postharvest losses.
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