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Search Results: 1 - 10 of 1866 matches for " Louise Kjaerulff "
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Identification of Four New agr Quorum Sensing-Interfering Cyclodepsipeptides from a Marine Photobacterium
Louise Kjaerulff,Anita Nielsen,Maria Mansson,Lone Gram,Thomas O. Larsen,Hanne Ingmer,Charlotte H. Gotfredsen
Marine Drugs , 2013, DOI: 10.3390/md11125051
Abstract: During our search for new natural products from the marine environment, we discovered a wide range of cyclic peptides from a marine Photobacterium, closely related to P. halotolerans. The chemical fingerprint of the bacterium showed primarily non-ribosomal peptide synthetase (NRPS)-like compounds, including the known pyrrothine antibiotic holomycin and a wide range of peptides, from diketopiperazines to cyclodepsipeptides of 500–900 Da. Purification of components from the pellet fraction led to the isolation and structure elucidation of four new cyclodepsipeptides, ngercheumicin F, G, H, and I. The ngercheumicins interfered with expression of virulence genes known to be controlled by the agr quorum sensing system of Staphylococcus aureus, although to a lesser extent than the previously described solonamides from the same strain of Photobacterium.
Proceedings of the Nineteenth Conference on Uncertainty in Artificial Intelligence (2003)
Christopher Meek,Uffe Kjaerulff
Computer Science , 2013,
Abstract: This is the Proceedings of the Nineteenth Conference on Uncertainty in Artificial Intelligence, which was held in Acapulco, Mexico, August 7-10 2003
Genetics of a sex-linked recessive red eye color mutant of the tarnished plant bug, Lygus lineolaris  [PDF]
Margaret Louise Allen
Open Journal of Animal Sciences (OJAS) , 2013, DOI: 10.4236/ojas.2013.32A001
Abstract: An inbred colony of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera), was observed to contain specimens with abnormal traits including red eyes, deformed antennae, and deformed legs. These specimens were isolated and back crossed to create stable phenotypic strains. The only successful strain established was a red eyed strain named Cardinal. The trait was more prevalent and stable in males, suggesting that it could be sex linked. To test the hypothesis that the trait was based on a recessive sex linked allele, classical genetic crosses were performed. The hypothesis was confirmed, and the eye color phenotype was measured and characterized using color analysis software. The trait is similar to other red eyed phenotypes described in this species, but is clearly based on a different mutation since it is sex linked rather than autosomal. The results of crossing experiments also suggest that inbreeding in this species results in substantial fitness cost to laboratory insects.
Genetics of a sex-linked recessive red eye color mutant of the tarnished plant bug, Lygus lineolaris  [PDF]
Margaret Louise Allen
Open Journal of Animal Sciences (OJAS) , 2013, DOI: 10.4236/ojas.2013.32A001
Abstract:

An inbred colony of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera), was observed to contain specimens with abnormal traits including red eyes, deformed antennae, and deformed legs. These specimens were isolated and back crossed to create stable phenotypic strains. The only successful strain established was a red eyed strain named Cardinal. The trait was more prevalent and stable in males, suggesting that it could be sex linked. To test the hypothesis that the trait was based on a recessive sex linked allele, classical genetic crosses were performed. The hypothesis was confirmed, and the eye color phenotype was measured and characterized using color analysis software. The trait is similar to other red eyed phenotypes described in this species, but is clearly based on a different mutation since it is sex linked rather than autosomal. The results of crossing experiments also suggest that inbreeding in this species results in substantial fitness cost to laboratory insects.

A Combinatorial Analysis of Tree-Like Sentences  [PDF]
Gilbert Labelle, Louise Laforest
Open Journal of Discrete Mathematics (OJDM) , 2015, DOI: 10.4236/ojdm.2015.53004
Abstract: A sentence over a finite alphabet A, is a finite sequence of non-empty words over A. More generally, we define a graphical sentence over A by attaching a non-empty word over A to each arrow and each loop of a connected directed graph (digraph, for short). Each word is written according to the direction of its corresponding arrow or loop. Graphical sentences can be used to encode sets of sentences in a compact way: the readable sentences of a graphical sentence being the sentences corresponding to directed paths in the digraph. We apply combinatorial equations on enriched trees and rooted trees, in the context of combinatorial species and Pólya theories, to analyze parameters in classes of tree-like sentences. These are graphical sentences constructed on tree-like digraphs.
Gold: A Unique Pigmentation Defective Laboratory Strain of the Lady Beetle  [PDF]
Margaret Louise Allen
Advances in Entomology (AE) , 2016, DOI: 10.4236/ae.2016.41002
Abstract: A laboratory colony of Coleomegilla maculata (De Geer) was selected for a novel phenotypic color trait. The phenotype was paler in color than the wild type, although not as pale as a previously described mutant strain, yellow (ye), and retained dark pigmentation in the eyes. This selected strain was named gold. Mendelian breeding experiments indicate a recessive biallelic inheritance. The strain has decreased fitness characteristics based on measurements of egg production and pupa size.
Air-Ground Temperature Coupling: Analysis by Means of Thermal Orbits  [PDF]
Vladimir Cermak, Louise Bodri
Atmospheric and Climate Sciences (ACS) , 2016, DOI: 10.4236/acs.2016.61009
Abstract: Long-term measurements of air, near-surface (soil) and ground temperatures that were collected between 1994 and 2013 at the drill site of the Geothermal Climate Change Observatory (Prague) were analyzed to understand the relationship between these variables and to reveal the mechanisms of heat transport at the land-atmosphere boundary layer. The 2D Thermal Orbit (TO) method was applied to detect regularities that were hidden in noisy and highly variable temperature time series. The results showed that the temperatures at shallow depths were affected by surface air temperature (SAT) variations on seasonal and annual time scales and could be regarded as an accurate proxy for low frequency temperature variations at the Earth’s surface. Only low-frequency/ high-amplitude surface temperature variations penetrate into the subsurface because of strong damping and the filtering effect of the ground surface. The borehole temperatures have good potential to capture temperature variations (periodicities) over long time scales that cannot be detected in the SAT series themselves because of the interference of higher frequency noise. The TO technique is a useful and powerful tool to quickly obtain diagnostics of the presence of long periodicities in borehole temperature time series.
Characteristics of a Laboratory Strain of Coleomegilla maculata with a Novel Heritable Wing Spot Pattern Trait  [PDF]
Margaret Louise Allen
Advances in Entomology (AE) , 2016, DOI: 10.4236/ae.2016.41007
Abstract: The lady beetle Coleomegilla maculata is a common New World insect that is naturally colored pink to red or orange with black spots on the forewings of the adult stage. Previous laboratory in-breeding resulted in selection for a strain lacking red pigment in the cuticle and eyes. An additional strain selected for a novel spotting pattern is described here. The inheritance of the new trait, “ten spotted” (10sp), was determined by classical crossing experiments. Inheritance of the trait was autosomal and exhibited incomplete dominance. Bionomic strain measurements were compared to the parental strains and were similar overall. Two expressed sequences from C. maculata that may be related to the new phenotype were compared to model insect genes encoding a melanin biosynthesis enzyme and a patterning transcription factor.
Pigmentation Restored in Mutant Laboratory Strain of the Lady Beetle Coleomegilla maculata through Dietary Supplementation  [PDF]
Margaret Louise Allen
Advances in Entomology (AE) , 2016, DOI: 10.4236/ae.2016.43014
Abstract: A laboratory colony of Coleomegilla maculata (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain to the diet. While the wings and other colored portions of the cuticle re-gained the red color, the eyes of the pigmentation deficient insects were not changed from the pale mutant form. Plant derived carotenes lycopene and beta-carotene did not restore the mutant beetles to a visibly distinguishable red color. An additional pigmentation deficient mutant strain, gold, partially recovered red cuticle color when provided with diet containing pigmented insect particles. This work represents the first rescue of a color phenotype in a lady beetle.
A Mutational Analysis of the Endophilin-A N-BAR Domain Performed in Living Flies
Anita G. Jung,Christina Labarerra,Anna M. Jansen,Klaus Qvortrup,Klemens Wild,Ole Kjaerulff
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009492
Abstract: Endophilin is a cytoplasmic protein with an important function in clathrin-dependent endocytosis at synapses and elsewhere. Endophilin has a BAR (Bin/Amphiphysin/Rvs-homology) domain, which is implicated in the sensing and induction of membrane curvature. Previous structure-function studies of the endophilin-A BAR domain have almost exclusively been made in reduced systems, either in vitro or ex vivo in cultured cells. To extend and complement this work, we have analyzed the role played by the structural features of the endophilin-A BAR domain in Drosophila in vivo.
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