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Search Results: 1 - 10 of 9850 matches for " Lorely; Ramos "
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Aplicación del análisis de riesgo a la producción de proteínas recombinantes expresadas en Escherichia coli
García,José; Santana,Zeila; Zumalacárregui,Lourdes; Quintana,Marisel; Milá,Lorely; Ramos,Marbel; Beldarraín,Alejandro;
Vaccimonitor , 2012,
Abstract: in this paper a risk analysis management is applied using the failure mode effects analysis to the fermentation processes that use e. coli as a host, to produce recombinant proteins with therapeutic, vaccinate or diagnostic aims. the analysis of the type and probability of occurrence of failures in the fermentation process, the evaluation of the impact in the quality of the product and the probability of detection of these failures are carried out. the severity, occurrence probability and detection probability are evaluated and the risk priority number is calculated. techniques used in quality assurance as brainstorming and ishikawa diagram were used. the potential causes that have higher influence in the failures of a fermentation process of recombinant e. coli are: inadequate handling during inoculation, presence of phages and unqualified personnel. actions to minimize the risks are proposed.
Production of a biological surfactant Producción de un tensoactivo biológico
Rosero N. Gladys,Dugarte Fanny,Pimienta Astrid Lorely,Díaz María Piedad
Revista Colombiana de Biotecnología , 2002,
Abstract: This paper summarizes the scale up work performed at the Colombian Petroleum Institute on a process to produce at pilot plant level a biosurfactant of the rhamnolipid type. By examination of both the activation conditions of the microorganism and design aspects of the broth, a stable condition was achieved which consistently triggers the production mechanisms and thus it was obtained a significant increment in biosurfactant productivity. The biological surfactant exhibited high efficiency in applications such as hydrocarbon biodegradation in saline environments, corrosion inhibition, and crude oil recovery from storage tank bottom sludges. En este artículo se resume el estudio realizado en el Instituto Colombiano del Petróleo (ICP), para escalar desde el laboratorio hasta la planta piloto, un proceso para la producción de biosurfactante tipo ramnolípido. Mediante el examen de las condiciones de activación del microorganismo y del dise o del medio de cultivo se establecieron condiciones que en forma sistemática disparan el mecanismo productor del microorganismo y permiten incrementar la productividad del biosurfactante. Se demostró la alta eficiencia de este aditivo biológico en aplicaciones, tales como biodegradación de hidrocarburos en medios salinos, control de la corrosión y recuperación de crudo a partir de lodos de fondos de tanques de almacenamiento.
Niveles de resistencia a insecticidas y sus mecanismos en una cepa de Aedes aegypti de Santiago de Cuba
María Magdalena Rodríguez,Juan A. Bisset,Lorely H. Milá,Eric Calvo
Revista Cubana de Medicina Tropical , 1999,
Abstract: Producto del más reciente brote de dengue en el municipio Santiago de Cuba, se estudió una cepa de este vector para determinar sus niveles de susceptibilidad y/o resistencia a insecticidas organofosforados y piretroides. Los resultados de los bioensayos mostraron bajos niveles de resistencia a fentión, malatión y deltametrina, se obtuvieron moderados para temefos, metil-pirimifos y cipermetrina, y altos para clorpirifos. Según los resultados obtenidos, en el uso del sinergista S,S,S tributil fosfotritiado, se demostró que las enzimas esterasas juegan una función importante en la resistencia a temefos y clorpirifos. Utilizando el sinergista piperonil butóxido se demostró que las enzimas oxidasas de función múltiple no intervienen en la resistencia a ninguno de los insecticidas evaluados. Se realizaron las técnicas bioquímicas para la detección de los mecanismos de resistencia mediado por enzimas esterasas, glutatión-s-transferasa (GST) y acetilcolinesterasas, (AchE) en Aedes aegypti y quedó demostrado, de acuerdo con los altos valores de frecuencia observados para cada uno de los mecanismos, que las enzimas esterasas y GST intervienen en la resistencia a insecticidas, no resultando así para la AchE. Sin embargo, por primera vez en Aedes aegypti, se encontró la presencia del gen de la AchE, aunque a baja frecuencia. Mediante electroforesis en gel de poliacrilamida se observó una banda fuertemente te ida con un valor de movilidad relativa de 0,779; la cual se nombró A4, que no se observó en la cepa de referencia, y que pudiera estar asociada con la resistencia a organofosforados, hecho que queda por demostrar en futuros trabajos. As a result of the most recent dengue outbreak in Santiago de Cuba province, a strain of this vector was studied to determine the levels of sensitivity and/or resistance to organophosphate and pyrethoid insecticides. The results of bioassays showed low levels of resistance to fention, malathion and deltametrine, moderate levels of resistance to temephos, metyl-pirimifos and cipermetrine and high levels of resistance to chlorpirifios. According to the results obtained from the use of S.S.S. phosphotrithiate trybutil synergist, it was shown that esterases play an important role in resistance to temephos and chlorpirifos. Piperonyl butoxide synergist disclosed that multifunction oxidases were not involved in the resistance to any of the evaluated insecticides. Biochemical techniques were applied to detect esterase-, glutathione-S- transferase- and acetylcholineaterase-mediated resistance mechanisms of Aedes aegypti. In accordance wit
PARAMETERS EXAMINATION OF A BIOSURFACTANT PRODUCTION AT LABORATORY SCALE
Neira-Gladys Rosero,Astrid-Lorely Pimienta,Fanny Dugarte,Fredy-Gonzalo Carvajal
CT&F - Ciencia, Tecnología y Futuro , 2003,
Abstract: This work presents the results obtained from the laboratory-scale experimentation for the optimization of production of rhamnolipid-type biosurfactant in a batch process, through the calculation and analysis of yield parameters. Different carbon/nitrogen ratios were studied, for which the production rates of rhamnolipid under nitrogen limitation was defined. Bacterial growth yield parameters Y X/N and Y X/C, were also calculated. Este trabajo presenta resultados de la fase de laboratorio, correspondientes a la optimización del proceso de producción de un biosurfactactante tipo ramnolípido, mediante la obtención y análisis de sus parámetros de rendimiento. Se estudiaron diferentes relaciones carbono/nitrógeno determinando la velocidad de producción de ramnolípido en condiciones de limitación por nitrógeno. También se estimaron los parámetros de rendimiento del crecimiento bacteriano Y X/N y Y X/C, logrados con los substratos de nitrógeno y carbono. Este trabalho apresenta resultados da fase de laboratório, correspondentes à otimiza o do processo de produ o de um biosurfatactante tipo ramnolipídio, mediante a obten o e análise dos seus parametros de rendimento. Estudaramse diferentes rela es carbono/nitrogênio determinando a velocidade de produ o de ramnolipídio em condi es de limita o por nitrogênio. Também se estimaram os parametros de rendimento do crescimento bacteriano Y X/N y Y X/C, conseguidos com os substratos de nitrogênio e carbono.
FACTORS DETERMINING THE PHYTOPLANKTON VARIABILITY IN THE SWAMPS OF THE MOMPOSINA DEPRESSION (COLOMBIA)
Yasmín Plata-Díaz,Astrid-Lorely Pimienta-Rueda
CT&F - Ciencia, Tecnología y Futuro , 2011,
Abstract: Description of the main environmental factors that structure phytoplankton dynamic in 27 swamps of the Momposina depression located in the area of influence of the Cicuco-Boquete oil field. Accor-ding to the results, the parameters that determined the variations in the physics and chemistry of the water are related to system hydrology, followed by the variables associated with the industrial operation, particularly in relation to chloride content and natural mineralization, which is due to conductivity and alkalinity. The euglenoids were the most common throughout the study, particularly during low water periods; cyanobacteria density increased in high waters and pennate diatoms in rising water. The lowest average diversity and richness were reported in the systems with the highest domestic and industrial impact as well as in the swamp referenced herein. Variations in phytoplankton composition were not related to the hydrological periods studied. pH, NO3, conducti-vity and the organic load (BOD5), as well as variables indirectly related to the industrial operation (chlorides and COD) were determining variables. Total hydrocarbons were not a significant variable in the community structure. Se describen los principales factores ambientales que estructuran la dinámica del fitoplancton en 27 ciénagas de la depresión Momposina, ubicadas en el área de influencia del campo petrolero Cicuco-Boquete. De acuerdo con los resultados, los parámetros que determinaron las variaciones en la física y química del agua están relacionados con la hidrología de los sistemas, seguida de las variables asociadas con la operación industrial, específicamente en relación al contenido de cloruros así como la mineralización natural, que puede ser explicada por la conductividad y la alcalinidad. Los euglenoideos fueron los de mayor ocurrencia a lo largo del estudio, particularmente en los períodos de aguas bajas; las cianobacterias aumentaron en densidad en aguas altas y las diatomeas pennadas proliferaron en los períodos de aguas en ascenso. Los más bajos promedios de diversidad y riqueza de especies, fueron registrados tanto en los sistemas con mayor impacto doméstico e industrial así como en la ciénaga de referencia. Las variaciones en la composición del fitoplancton no estuvieron relacionadas con los periodos hidrológicos estudiados. El pH, los NO3, la conductividad y carga orgánica (DBO5), así como las variables indirectamente relacionadas con la operación industrial (cloruros y DQO) fueron las variables determinantes. Los hidrocarburos totales no constituyeron una variable signi
Niveles de resistencia a insecticidas y sus mecanismos en una cepa de Aedes aegypti de Santiago de Cuba
Rodríguez,María Magdalena; Bisset,Juan A.; Milá,Lorely H.; Calvo,Eric; Díaz,Cristina; Alain Soca,Lázaro;
Revista Cubana de Medicina Tropical , 1999,
Abstract: as a result of the most recent dengue outbreak in santiago de cuba province, a strain of this vector was studied to determine the levels of sensitivity and/or resistance to organophosphate and pyrethoid insecticides. the results of bioassays showed low levels of resistance to fention, malathion and deltametrine, moderate levels of resistance to temephos, metyl-pirimifos and cipermetrine and high levels of resistance to chlorpirifios. according to the results obtained from the use of s.s.s. phosphotrithiate trybutil synergist, it was shown that esterases play an important role in resistance to temephos and chlorpirifos. piperonyl butoxide synergist disclosed that multifunction oxidases were not involved in the resistance to any of the evaluated insecticides. biochemical techniques were applied to detect esterase-, glutathione-s- transferase- and acetylcholineaterase-mediated resistance mechanisms of aedes aegypti. in accordance with the high frequency values observed in each of the mechanisms, it was proved that esterases and glutathione-s-transferase were involved in the insecticide resistance but acetylcholinesterases were not. however, acetylcholinesterase gen was found in aedes aegypti for the first time though at low frequency. the polyacrylamide-gel electrophoresis made it possible to observe a well-stained band with a relative mobility value of 0,779; this band was called a4 it was not observed in the reference strain and may be associated to organophosphate resistance which remains to be proved in future research.
Evaluación institucional de necesidades y oportunidades de apoyo psicológico en un hospital infantil
Lorely I. Miranda Martínez,María Eugenia del Río Rendón,Lucía Ledesma Torres
Ense?anza e Investigación en Psicología , 2009,
Abstract: El presente trabajo presenta las acciones puestas en práctica para detectar las necesidades de apoyo psicológico en un hospital infantil, con el fin de dise ar posteriormente las intervenciones apropiadas. Se distingue entre necesidades de apoyo, definidas profesionalmente y a partir de las necesidades sentidas por usuarios, y oportunidades, las cuales son acciones de interés no prioritarias para la institución y el equipo evaluador. Se emplea una estrategia multimétodo, a partir del modelo de inventario de recursos y el modelo de discrepancias. Los resultados se orientan a la creación en el hospital de una unidad de atención, ense anza e investigación en psicología de la salud.
Arequipa’s Population Perception in Regard to the Infrastructure of the Avenues Venezuela and Daniel Alcides Carrión  [PDF]
Yampier F. Ramos Polanco
Journal of Transportation Technologies (JTTs) , 2015, DOI: 10.4236/jtts.2015.51004
Abstract: The present study corresponds to a descriptive research, whose objective seeks to determine the level of satisfaction of the population of Arequipa city, in respect of public works infrastructure executed in the Avenues Venezuela and Daniel Alcides Carrion. We designed a survey questionnaire which was applied to 384 people randomly for convenience, in the areas surrounding the road interchange. The results showed that a 65.10% believed that the infrastructure works had a positive impact, in addition to a 52.86% and 7.29% of people who were satisfied and very satisfied respectively, with the vial exchange carried out in the Avenues Venezuela and Daniel Alcides Carrion.
Strategies in tower solar power plant optimization
A. Ramos,F. Ramos
Mathematics , 2012,
Abstract: A method for optimizing a central receiver solar thermal electric power plant is studied. We parametrize the plant design as a function of eleven design variables and reduce the problem of finding optimal designs to the numerical problem of finding the minimum of a function of several variables. This minimization problem is attacked with different algorithms both local and global in nature. We find that all algorithms find the same minimum of the objective function. The performance of each of the algorithms and the resulting designs are studied for two typical cases. We describe a method to evaluate the impact of design variables in the plant performance. This method will tell us what variables are key to the optimal plant design and which ones are less important. This information can be used to further improve the plant design and to accelerate the optimization procedure.
Heliostat blocking and shadowing efficiency in the video-game era
A. Ramos,F. Ramos
Mathematics , 2014,
Abstract: Blocking and shadowing is one of the key effects in designing and evaluating a thermal central receiver solar tower plant. Therefore it is convenient to develop efficient algorithms to compute the area of an heliostat blocked or shadowed by the rest of the field. In this paper we explore the possibility of using very efficient clipping algorithms developed for the video game and imaging industry to compute the blocking and shadowing efficiency of a solar thermal plant layout. We propose an algorithm valid for arbitrary position, orientation and size of the heliostats. This algorithm turns out to be very accurate, free of assumptions and fast. We show the feasibility of the use of this algorithm to the optimization of a solar plant by studying a couple of examples in detail.
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