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Search Results: 1 - 10 of 80501 matches for " Liu Xifu "
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Some results on the partial orderings of block matrices
Liu Xifu,Yang Hu
Journal of Inequalities and Applications , 2011,
Abstract: Some results relating to the block matrix partial orderings and the submatrix partial orderings are given. Special attention is paid to the star ordering of a sum of two matrices and the minus ordering of matrix product. Several equivalent conditions for the minus ordering are established. Mathematics Subject Classification (2000): 15A45; 15A57
Chemotherapy of multidrug-resistant human lung cancer combined with an MDR1 ribozyme retroviral vector in an orthotopic model
Zhenqiang Gao,Zhiping Gao,Xifu Liu
Chinese Science Bulletin , 1997, DOI: 10.1007/BF02882442
Abstract:
Reversal of drug resistance of multidrug-resistant human lung cancer cells by an MDR1 ribozyme
Zhenqiang Gao,Zhiping Gao,Xifu Liu
Chinese Science Bulletin , 1997, DOI: 10.1007/BF02882525
Abstract:
Reversal of drug resistance in multidrug-resistant human lung cancer cells by MRP antisense RNA mediated by retrovirus
Zhenqiang Gao,Zhiping Gao,Xifu Liu
Chinese Science Bulletin , 1997, DOI: 10.1007/BF03182793
Abstract:
Chemotherapy of multidrug-resistant human lung cancer combined with an MDR1 ribozyme retroviral vector in an orthotopic model

Gao Zhenqiang,Gao Zhiping,Liu Xifu,

科学通报(英文版) , 1997,
Abstract:
Reversal of drug resistance of multidrug-resistant human lung cancer cells by an MDR1 ribozyme

Zhenqiang Gao,Zhiping Gao,Xifu Liu,

科学通报(英文版) , 1997,
Abstract:
Selective gene therapy for human lung adenocarcinoma by tumor-specific expression of herpes simplex virus thymidine kinase gene
Zhenqiang Gao,Zhiping Gao,Tao Zhang,Xifu Liu
Science China Life Sciences , 1997, DOI: 10.1007/BF02881738
Abstract: According to the fact that CEA gene expressed only in lung adenocarcinoma but not in normal lung cells, a retroviral expression vector (pCEATK) of the herpes simplex virus thymidine kinase (HSV-TK) gene regulated by CEA promoter was constructed and introduced into CEA-producing human lung adenocarcinoma cells GL and non-CEA-producing HeLa cells. The expression of pCEATK and Ganciclovir (GCV) sensitivity of the transfected cells were testedin vitro andin vim. pCEATK expressed only in CEA-producing GL cells but not in non-CEA-producing HeLa cells. The sensitivity to GCV of pCEATK-transfected GL was 992 times higher compared with that of the parental cell line and there was obvious “bystander effect”in vitro. HeLa cells transfected wtih pCEATK were still resistant to GCV. Injection of GCV resulted in significant regression of pCEATK-transfected GL tumors in nude mice. In addition, all mice with any fraction of GL cells expressing HSV-TK exhibited a significant reduction in tumor growth, including mice with only 10% of GL cells expressing HSV-TK. These results show the possibility of HSV-TK gene-drug therapy using the tumor-specific promoter of CEA gene against CEA-producing lung cancers which was usually refractory to conventional chemotherapy.
Increasing drug resistance in human lung cancer cells by mutant-type p53 gene mediated by retrovirus
Zhenqiang Gao,Zhiping Gao,Xifu Liu,Tao Zhang
Science China Life Sciences , 1997, DOI: 10.1007/BF02879112
Abstract: Human mutant-type (mt) p53 cDNA was synthesized and cloned from human lung cancer cell line GL containing mt-p53 gene by using polymerase chain reaction (PCR). It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis. The retroviral vector pD53M of the mt-p53 was constructed and introduced into the drug-sensitive human lung cancer cells GAO in which p53 gene did not mutate. The transfected GAO cells strongly expressed mutant-type p53 protein by immunohistochemistry, showing that pD53M vector could steadily express in GAO cells. The drug resistance to several anticancer agents of GAO cells infected by pD53M increased in varying degrees, with the highest increase of 4-fold,in vitro andin vivo. By quantitative PCR and flow cytometry (FCM) analyses, the expression of MDR1 gene and the activity of P-glycoprotein (Pgp) did not increase, the expression of MRP gene and the activity of multidrug resistance-related protein (Mrp) increased slightly. These results indicated that the drug resistance associated with mt-p53 gene might be somewhat correlated with MRP/Mrp but not with MDR1/Pgp. It was possible to modify the tumor drug resistance by changing status of p53 gene.
Selective reversal of drug resistance in drug-resistant lung adenocarcinoma cells by tumor-specific expression of mdrl ribozyme gene mediated by retrovirus
Zhenqiang Gao,Zhiping Gao,Xifu Liu,Tao Zhang
Science China Life Sciences , 1997, DOI: 10.1007/BF02882039
Abstract: According to the fact that CEA gene expressed only in lung adenocarcinoma and not in normal lung cells, a retroviral vector (pCEAMR) was constructed which carried the CEA promoter coupled to MDRl ribozyme gene. pCEAMR was introduced into drug-resistant lung adenocarcinoma cells GAOK with CEA expression and HeLaK without CEA expression; the expression of pCEAMR and drug resistance in the infected cells were analyzedin vitro andin vivo; pCEAMR expressed only in CEA-producing GAOK cells and not in non-CEA-producing HeLa cells. The drug resistance to doxorubicin (DOX) decreased 91.5% in the infected GAOK cells and did not change in the infected HeLa cells. In nude mice, DOX could obviously inhibit the growth of the infected GAOK tumors, and had no effect on the growth of the infected HeLa cells. These results indicated that MDRl ribozyme gene regulated by CEA promoter expressed only in human adenocarcinoma cells and reversed their drug resistance selectively. This gene-drug therapy might serve as an effective treatment method for patients with CEA-producing lung cancers which was usually refractory to conventional chemotherapy
Selective reversal of drug resistance in drug-resistant lung adenocarcinoma cells by tumor-specific expression of MDR1 ribozyme gene mediated by retrovirus

GAO Zhenqiang,GAO Zhiping,LIU Xifu,ZHANG Tao,

中国科学C辑(英文版) , 1997,
Abstract: According to the fact that CEA gene expressed only in lung adenocarcinoma and not in normal lung cells, a retroviral vector (pCEAMR) was constructed which carried the CEA promoter coupled to MDRl ribozyme gene. pCEAMR was introduced into drug-resistant lung adenocarcinoma cells GAOK with CEA expression and HeLaK without CEA expression; the expression of pCEAMR and drug resistance in the infected cells were analyzedin vitro andin vivo; pCEAMR expressed only in CEA-producing GAOK cells and not in non-CEA-producing HeLa cells. The drug resistance to doxorubicin (DOX) decreased 91.5% in the infected GAOK cells and did not change in the infected HeLa cells. In nude mice, DOX could obviously inhibit the growth of the infected GAOK tumors, and had no effect on the growth of the infected HeLa cells. These results indicated that MDRl ribozyme gene regulated by CEA promoter expressed only in human adenocarcinoma cells and reversed their drug resistance selectively. This gene-drug therapy might serve as an effective treatment method for patients with CEA-producing lung cancers which was usually refractory to conventional chemotherapy
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