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Search Results: 1 - 10 of 148781 matches for " Liu Ruiqing Nie Wenhui Chen Yuze "
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Liu Ruiqing Nie Wenhui Chen Yuze,

动物学研究 , 1995,
Abstract: The karyotype of Martes foina intermedia were studied by C-banding and silver staining. The number of diploid chromosome is 38. Autosomes consist of 7 pairs of metacentrics, 2 pairs of submetacentrics, 9 pairs of subacrocentrics. Chromosome No.18 has a secondary constriction near by centromere on the long arm. X chromosome is a metacentrics and of a size between that of chromosome No.3 and No.4, and the Y, the smallest acrocentric.The centromeric distribution of heterochromatin has been demonstrated in most of chromosomes. In some of chromosome centromericregions are stained slightly C-bands. Moreover, the constitutive heterochromatin of centromere region of chromosome No.1 is apparently disappeared. The whole short arm of chromosome No.9 is found to be a complete heterochromatin one. The secondary constriction region of chromosome No.18 is with heterochromatin. The uncommon distribution of interstitial heterochromatin has observed on the long arm of X chromosome. Y chromosome is completely C-band positive.There is only one pair of Ag-NORs in the silver-stained karyotype, it is located at the secondary constriction of chromosom No.18. These specific distribution of C-bands and relative to karyotype evolution of Martes foina intermedia were discussed.

Wang Wen,Su Bing,Lan Hong,Liu Ruiqing,Zhu Chunling Nie Wenhui,Chen Yuze,Zhang Yaping,

动物学研究 , 1996,
Abstract: Restriction maps of ribosomal DNA repetypes of the three putative slow lorises species, Nycticebus coucang, N. intermedius and N. pygmaeus, were constructed with 15 restriction endonucleases and 285, 185 rDNA probes cloned from human. The site numbersmapped on the non-transcribed spacers for N. coucang, N. intermedius, and N.Mpygmaeus, were 23, 24, and 24 respectively. N. coucang differs from N. intermidius at 13 sites, and 14 sites from N. Pygmaeus. There are only one site difference between N. intermedius and N. pygmaeus. The sequence divergence between N. coucang and N. intermedius was estimated as 12.65%, and that between the former and N. Pygmaeus was 14.24%. However, N. intermedius and N. Pygmaeus have only 0.71 % sequence divergence. Integrating information from morphology, karyotype, mitochondrial DNA and ribosomal DNA variation, we refer to the hypothesis that there are two valid species in the genus Nycticebus, namely, N. coucang and N. Pygmaeus while N. intermedius may be at the level of semispecies of N. pygmaeus at the most. The giant difference between N. coucang and N. pygmaeus implies that the former has strongly diverged from the latter.

Liu Ruiqing,Nai Wenhui,Chen Yuze,Yu Dinghui,

动物学研究 , 1996,
Abstract: White-cheeked gibbon(H. leucogenys) is distributed in the south of Yunnan, China.The chromosomes of a female and a male were studied by several banding techniques(G-,C -and Ag-staining), and a techniqe for simultaneous exhibiting chromosome replication patterns and late replicating Y chromosome. The diploid chromosome number is 52. There are 22 pairs of metacentrlcs or submetacentrics (No. 1-22); 3 pairs of acrocentrics(Nos. 23-25), and chromosomes Nos. 23-24 with a secondary constriction near the centromere on the short arm.The X chromosome is a large metacentric, and the Y, the smallest acrocentric. Its karyotype for mula is 44(M or SM)+ 6(A), XY(M, A). The centrimeric distribution of heterochromatin has been demonstrated in some chromosomes. In addion, there are 3 kinds of heterochromatin on the chromosomes:(1) ab sent of centromeric heterochromatin,(2) interstitial heterochromatin,(3) terminal heterochromatin.Interstitial heterochromatin has been observed on the blarm of X chromosome. Y chromosome is completely C-band positive. Silver staining revealed 5 chromosomes with NORs in the male specimen.4 Ag-NORs of theth are heated on the secondary constriction of chromosomes 23 and 24 respectivly,and one of them is located on the Y chromosome which has been confirmed by Fluorescence in situ hybridization(FISH) in the same cell. There are 4 chromosomes with Ag-NORs in the female specimen,and they are also located on the secondary constriction of chromosomes 23 and 24. Moreover,the association of Ag-NORs have been observed. Base on the above results,the relationship in gibbons and the way of chromosome evo lution in the genus Hylobates have been discussed.

Liu Ruiqing,Chen Yuze,Shi Liming,

动物学研究 , 1994,
Abstract: This paper deals with the comparison on karyotypes between red goral (Naemorhedus cranbrooki) and goral(Naemorhedus goral griseus).Their chromosomes were studied by several banding techniques(G-,C-and Ag-staining).These results are given as below:(1) The diploid number of red goral(N.cranbrooki) is 56.All of the chromosomes are acrocentrics.The X is a large one, and the Y,the smallest.The goral(N.g.griseus)has a dipolid number of 2n=54.The karyotype are consisted of 26 pairs of acrocentrics and one pair of submetacentric.The X chromosome is also a large acrocentric,and the Y chromosome,the smallest acrocentric.Therefor,the existence of submetacentric elements makes the remarkable difference between the chromosomal structures of red goral and goral.But autosomal arm number(N.F) are all 54 for both gorals.(2)The G-banding patterns of the 3q in goral are similar to that of the No.3 in red goral,and the G-banding patterns of the 3p in goral are similar to that of the No.27 in red goral.Therefore,it is suggested that a Robertsonian translocation could have happened during the karyotype evolution of gorals.(3)The centromeric distribution of heterochromatin has been demonstrated in all chromosomes of red goral and goral,while the whole Y chromosome is C-band positive.The C-bands of chromosomes are also similar for both gorals.(4) Ag-NORs have been observed at the terminal of 3 pairs of acrocentrics in red goral and goral,which could be No.l,2 and 4 larger acrocentrics according to their size and morphology.These results suggest a close relationship between red goral (N.cranbrooki) and goral (N.g.griseus).

LIU Ruiqing SHI Liming CHEN Yuze,

动物学研究 , 1987,
Abstract: The chromoeomes and nucleoli, micronucleoli in prophase of oocytes from human fcotal were studied by Giemsa and silver-staining.There arc profound changes of epiralization, despiralization, respiralization, redespiralization in the chromosomal morphology from preleptotene, diplotene, to dictyotone. The bivalents are uncoiled, extremely extended in dictyotene stage, and morphologically they arc "lampbrush-like". The morphology and number of primary nucleoli, micronucleoli. as well as silver-tained small clots in various stages of prophase are also described.
Electronic Strengthening of Graphene by Charge Doping
Chen Si,Wenhui Duan,Zheng Liu,Feng Liu
Physics , 2012, DOI: 10.1103/PhysRevLett.109.226802
Abstract: Graphene is known as the strongest 2D material in nature, yet we show that moderate charge doping of either electrons or holes can further enhance its ideal strength by up to ~17%, based on first principles calculations. This unusual electronic enhancement, versus conventional structural enhancement, of material's strength is achieved by an intriguing physical mechanism of charge doping counteracting on strain induced enhancement of Kohn anomaly, which leads to an overall stiffening of zone boundary K1 phonon mode whose softening under strain is responsible for graphene failure. Electrons and holes work in the same way due to the high electron-hole symmetry around the Dirac point of graphene, while over doping may weaken the graphene by softening other phonon modes. Our findings uncover another fascinating property of graphene with broad implications in graphene-based electromechanical devices.
Superconducting Graphene: the conspiracy of doping and strain
Chen Si,Zheng Liu,Wenhui Duan,Feng Liu
Physics , 2013,
Abstract: Graphene has exhibited a wealth of fascinating properties, but is also known not to be a superconductor. Remarkably, we show that graphene can be made a conventional Bardeen-Cooper-Schrieffer superconductor by the combined effect of charge doping and tensile strain. While the effect of doping is obvious to enlarge Fermi surface, the effect of strain is profound to greatly increase the electron-phonon coupling. At the experimental accessible doping (4E+14cm-2) and strain (~16%) levels, the superconducting critical temperature Tc reaches as high as ~30 K, the highest for a single-element material above the liquid hydrogen temperature. This significantly makes graphene a commercially viable superconductor.

Lan Hong,Chen Zhiping,Liu Ruiqing,

动物学研究 , 1996,
Abstract: 本实验用ApaⅠ,BglⅠ,BglⅡ,ClaⅠ,EcoRⅠ,EcoRⅤ,HindⅢ,HpeⅠ,PstⅠ,PvuⅠⅡ,SacⅠ,SalⅠ等12种限制性内切酶分析猪獾和黄鼬的mtDNA限制性片段,并用双酶解法构建限制性内切酶图谱。结合以往积累的资料,我们对哺乳动物mtDNA限制性位点在远缘物种间的保守性和变异性进行了初步讨论。

Chen Zhiping,Liu Ruiqing,Li Chongyun,Wang Yingxiang,

动物学研究 , 1996,
Abstract: The karyotypes of three species of wood mice have been studied by banding techniques(G-, C- and Ag-staining). Three species have the same diploid number, 2n=48. Allchromosomes in the complement of A. draco are acrocentrics. The karyotype of A.peninsulae consists of 22 pairs of acrocentrics, 1 pair of metacentrics, X and Y areacrocentrics. However, the karyotype of A. latronum consists of 13 pairs of acroentrics, 2pair of subacrocentrics, 1 pair of submetacentric, 7 pairs of metacentrics, X chromosome isa acrocentric.The G-banded, C-banded and silver-stained karyotypes have been observed. G-banding allows to identify all chromosome pairs in three species. C-banding reveals centromericheterochromatin in all chromosomes in A. draco. In the C-banded karyotype of A.peninsulae, the centromeric heterochromatin is found in all chromosomes, however, thecentromeric C-bands of Nos.7, 11, 15, 21, 22 are very faultly stained even negative,there are also telomeric C-bands in Nos.2, 4, 7. The Y chromosomes of A. draco and A.peninsulae are whole heterochromatin. The centromeric C-bands of all chromosomes are positive in the C-banded karyotype of A. latronum, but the centromeric C-bands in Nos.3,4, 10, 12, 13 are very faultly stained. Ag-NORs are predominantly located at Nos.7, 8,18, 21, 22 in A. draco. While Ag-NORs are found in Nos.7, 8 in A. peninsulae and A.latronum, respectively. The polymorphism of Ag-NORs of No.22 (in A. draco) and No.7 (inA. peninsulae and A. latronum) is found. Moreover, the classification of three species has also been discussed.
Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori  [PDF]
Zhengbing Lv, Yunlong Liu, Ying Chen, Bo Li, Lili Liu, Jian Chen, Zuoming Nie, Qing Sheng, Wei Yu, Yaozhou Zhang
American Journal of Molecular Biology (AJMB) , 2012, DOI: 10.4236/ajmb.2012.23023
Abstract: According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained its ORF for further study by bioinformatics analysis. It is 444 bp and encodes 147 amino acid residues, with a predicted molecular weight (MW) of 16.4 kD and an isoelectric point (pI) of 3.69. In this study, we successfully constructed a recombinant plasmid pET-28a(+)-Bm-X and expressed it in Escherichia coli. We used the fusion protein rBm-X which purified by Niaffinity chromatography to produce polyclonal antibodies against Bm-X for Western blot analysis. The analysis revealed that Bm-X was expressed in the larval midgut, the epidermis and the silk gland. In addition, the subcellular localization analysis of silkworm ovary epithelial cells (BmN cells) showed that Bm-X protein was located both in cytoplasm and nucleus, and the signal was stronger in cytoplasm than in nucleus. Our findings indicate that Bm-X gene is a novel species-specificity gene and its expression product can be detected in tissues of the fifth silkworm instar larvae and BmN cells.
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