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Search Results: 1 - 10 of 38097 matches for " Lin Lihuang "
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Conservation and implications of eukaryote transcriptional regulatory regions across multiple species
Lin Wan, Dayong Li, Donglei Zhang, Xue Liu, Wenjiang J Fu, Lihuang Zhu, Minghua Deng, Fengzhu Sun, Minping Qian
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-623
Abstract: We constructed a hierarchical stochastic language (HSL) model for the identification of core TRRs in yeast based on regulatory cooperation among TRR elements. The HSL model trained based on yeast achieved comparable accuracy in predicting TRRs in other species, e.g., fruit fly, human, and rice, thus demonstrating the conservation of TRRs across species. The HSL model was also used to identify the TRRs of genes, such as p53 or OsALYL1, as well as microRNAs. In addition, the ENCODE regions were examined by HSL, and TRRs were found to pervasively locate in the genomes.Our findings indicate that 1) the HSL model can be used to accurately predict core TRRs of transcripts across species and 2) identified core TRRs by HSL are proper candidates for the further scrutiny of specific regulatory elements and mechanisms. Meanwhile, the regulatory activity taking place in the abundant numbers of ncRNAs might account for the ubiquitous presence of TRRs across the genome. In addition, we also found that the TRRs of protein coding genes and ncRNAs are similar in structure, with the latter being more conserved than the former.The identification of transcriptional regulatory elements that control the expression of each transcript is a fundamental and challenging problem in biology. Although tremendous progress has been made, both experimentally and computationally, the regulatory elements of genes are still not well understood, and only a handful of them have so far been experimentally verified [1]. The computational identification of regulatory elements is difficult for several reasons. First, genomic regulatory elements are usually short and degenerate [2]. Second, they are usually distributed across large genomic regions from the distant 5' upstream regions to the 3' downstream regions [3]. Meanwhile, ENCODE, a pilot project to identify all functional elements in the human genome sequence, has revealed that at least 93% of the human genome is transcribed in different cells and that
Molecular evidence on maize specific DNA fragment transferred into wheat through sexual hybridization
Chunxian Chen,Lihuang Zhu,Jingsan Sun
Science China Life Sciences , 1998, DOI: 10.1007/BF02882716
Abstract: Nearly 100 maize-specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis of two wheat DH populations from wheat and maize crosses. The result showed that a maize fragment in clone MR64 was transferred into two wheat DH lines, i.e. No. 18 in common wheat DHs and No. 15 in persicum wheat DHs, in which strong hybridization signals were detected. It is first proved at DNA level that some maize-specific DNA can be transferred into wheat DH progenies through fertilization at low frequency. Sequencing revealed that the size of the cloned insert DNA is 695 bp, containing 58% A + T. Southern hybridization to maize genome with several restriction enzymes indicated that MR64 is a dispersed repeat with 2–3 dominant tandem units. Its sequence structure, methylation pattern, copy number and chromosomal distribution were also determined. The possible mechanism of alien DNA transfer was discussed.
Molecular evidence on maize specific DNA fragment transferred into wheat through sexual hybridization

CHEN ChunxianZHU Lihuang SUN Jingsan,

中国科学C辑(英文版) , 1998,
Abstract: Nearly 100 maize specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis of two wheat DH populations from wheat and maize crosses. The result showed that a maize fragment in clone MR64 was transferred into two wheat DH lines, i.e. No.18 in common wheat DHs and No.15 in persicum wheat DHs, in which strong hybridization signals were detected. It is first proved at DNA level that some maize specific DNA can be transferred into wheat DH progenies through fertilization at low frequency. Sequencing revealed that the size of the cloned insert DNA is 695 bp, containing 58% A+T. Southern hybridization to maize genome with several restriction enzymes indicated that MR64 is a dispersed repeat with 2-3 dominant tandem units. Its sequence structure, methylation pattern, copy number and chromosomal distribution were also determined. The possible mechanism of alien DNA transfer was discussed.
Molybdenum Phosphide Flakes Catalyze Hydrogen Generation in Acidic and Basic Solutions  [PDF]
Zhongzhong Chen, Cuncai Lv, Zhibo Chen, Lihuang Jin, Jie Wang, Zhipeng Huang
American Journal of Analytical Chemistry (AJAC) , 2014, DOI: 10.4236/ajac.2014.517127
Abstract: Molybdenum phosphide (MoP) flakes were synthesized by the reduction of hexaammonium heptamolybdate tetrahydrate and ammonium dihydrogen phosphate. The flakes are porous and constructed by MoP nanoparticles with ca. 100 nm diameters. The lateral size of flakes ranges from less than 1 μm to larger than 5 μm, and the thickness of MoP fakes is ca. 200 nm. The mixture of MoP flakes and carbon black exhibits effective catalytic activity in the hydrogen evolution reaction. The optimal overpotential required for 20 mA·cm﹣2 current density is 155 mV in acidic solution and 184 mV in basic solution. The mixture can work stably in long-term hydrogen generation in both acidic and basic solution. The faradaic yield of mixture in hydrogen evolution reaction is nearly 100% in both acidic and basic solution. The Mo and P species in MoP flakes are found to have small positive and negative charge, respectively. The catalytic activity of MoP flakes is likely to be correlated with this charged nature.
Design and characterization of a W-band system for modulated DNP experiments
Mallory L. Guy,Lihuang Zhu,Chandrasekhar Ramanathan
Physics , 2015, DOI: 10.1016/j.jmr.2015.09.011
Abstract: Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94 GHz with a bandwidth greater than 1 GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mode, it is possible to limit the losses to 1 dB across a 2 GHz bandwidth. The loss is dominated by the presence of a quartz window used to isolate the waveguide pipe. This performance is comparable to systems with corrugated waveguide or quasi-optical components. The overall excitation bandwidth of the probe is seen to be primarily determined by the final antenna or resonator used to excite the sample and its coupling to the NMR RF coil. Understanding the instrumental limitations imposed on any modulation scheme is key to understanding the observed DNP results and potentially identifying the underlying mechanisms. We demonstrate the utility of our design with a set of triangular frequency-modulated DNP experiments.
Co9S8 Nanotubes as an Efficient Catalyst for Hydrogen Evolution Reaction in Alkaline Electrolyte  [PDF]
Lihuang Jin, Cuncai Lv, Jie Wang, Han Xia, Yaoxing Zhao, Zhipeng Huang
American Journal of Analytical Chemistry (AJAC) , 2016, DOI: 10.4236/ajac.2016.72018
Abstract: Cobalt sulfide (Co9S8) nanotubes were found to be an electrocatalyst for the hydrogen evolution reaction under alkaline condition. An electrode comprising of Co9S8 nanotubes on a glass carbon electrode (GCE) (mass loading: 0.855 mg·cm-2) produced a cathodic current density of 20 mA·cm-2 at an overpotential of 320 mV. The Co9S8/GCE electrode was stable over 20,000 s during potentiostatic electrolysis. Minor degradation of reduction current after 4000 cyclic voltammetric sweeps suggests the long-term viability under operating conditions. The faradaic efficiency of Co9S8 nanotubes is nearly 100% during the electrolysis of water.
Sense and antisense OsDof12 transcripts in rice
Dejun Li, Chunhua Yang, Xiaobing Li, Guobiao Ji, Lihuang Zhu
BMC Molecular Biology , 2008, DOI: 10.1186/1471-2199-9-80
Abstract: OsDof12, being a single copy gene located on rice chromosome 3, encodes a predicted Dof protein of 440 amino acids with one intron of 945 bp. The antisense transcript, OsDofl2os, overlaps with both the exonic and intronic regions of OsDof12 and encodes a functionally unknown protein of 104 amino acids with no intron. The sense-antisense OsDof12 transcripts were co-expressed within the same tissues, and their expressions were not tissue-specific in general. At different developmental stages in rice, the OsDof12 and OsDof12os transcripts exhibited reciprocal expression patterns. Interestingly, the expression of both genes was significantly induced under drought treatment, and inhibited by dark treatment. In the ProOsDof12-GUS and ProOsDof12os-GUS transgenic rice plants, the expression profiles of GUS were consistent with those of the OsDof12 and OsDof12os transcripts, respectively. In addition, the analysis of cis-regulatory elements indicated that either of the two promoters contained 74 classes of cis-regulatory elements predicted, of which the two promoter regions shared 53 classes.Based on the expression profiles of OsDof12 and OsDof12os, the expression patterns of GUS in the ProOsDof12-GUS and ProOsDof12os-GUS transgenic rice plants and the predicted common cis-regulatory elements shared by the two promoters, we suggest that the co-expression patterns of OsDof12 and OsDof12os might be attributed to the basically common nature of the two promoters.The gene regulation by natural antisense RNA in prokaryotes has been known for many years [1,2]. The first example was found in the plasmid ColE1, in which DNA replication was regulated by an antisense RNA [3,4]. Later, the natural antisense RNAs involved in the regulation of gene expression were also identified in a number of eukaryotes [5,6] including plants [7] and animals [8]. In the past few years, many regulatory RNA molecules have been characterized in eukaryotes [9,10]; one class of such regulatory RNA is the nat
Strigolactones are a new-defined class of plant hormones which inhibit shoot branching and mediate the interaction of plant-AM fungi and plant-parasitic weeds
CaiYan Chen,JunHuang Zou,ShuYing Zhang,David Zaitlin,LiHuang Zhu
Science China Life Sciences , 2009, DOI: 10.1007/s11427-009-0104-6
Abstract: Because plants are sessile organisms, the ability to adapt to a wide range of environmental conditions is critical for their survival. As a consequence, plants use hormones to regulate growth, mitigate biotic and abiotic stresses, and to communicate with other organisms. Many plant hormones function pleiotropically in vivo, and often work in tandem with other hormones that are chemically distinct. A newly-defined class of plant hormones, the strigolactones, cooperate with auxins and cytokinins to control shoot branching and the outgrowth of lateral buds. Strigolactones were originally identified as compounds that stimulated the germination of parasitic plant seeds, and were also demonstrated to induce hyphal branching in arbuscular mycorrhizal (AM) fungi. AM fungi form symbioses with higher plant roots and mainly facilitate the absorption of phosphate from the soil. Conforming to the classical definition of a plant hormone, strigolactones are produced in the roots and translocated to the shoots where they inhibit shoot outgrowth and branching. The biosynthesis of this class of compounds is regulated by soil nutrient availability, i.e. the plant will increase its production of strigolactones when the soil phosphate concentration is limited, and decrease production when phosphates are in ample supply. Strigolactones that affect plant shoot branching, AM fungal hyphal branching, and seed germination in parasitic plants facilitate chemical synthesis of similar compounds to control these and other biological processes by exogenous application.
Analysis of rice blast resistance genes by QTL mapping
Jichen Xu,Jiulin Wang,Zhongzhuan Ling,Lihuang Zhu
Chinese Science Bulletin , 2004, DOI: 10.1007/BF02900315
Abstract: Resistance to rice blast pathogen mostly shows a quantitative trait controlled by several minor genes. Its complexity and the mutable characteristic of rice blast isolates both hinder the development of the blast resistance research. The article here tried to explore the resistance gene distribution on rice chromosomes and the way of function. Totally 124 QTLs have been identified against 20 isolates using Cartographer software with a ZYQ8/JX17 DH population, which separately are at 100 loci of 72 marker intervals on 12 rice chromosomes. Of them, 16 QTLs were determined by the isolate HB-97-36-1. 82 QTLs (66.13%) are from the resistant parent alleles, ZYQ8, while 42 QTLs (33.87%) are from the susceptible parent alleles, JX17. In comparison of their positions on chromosome, most QTLs are clustered together and distributed nearby the major genes especially the regions on chromosomes 1, 2, 8, 10 and 12. Each QTL could account for the resistance variation between 3.52%–68.64%. And, a positional QTL might display the resistance to several different isolates with different contributions.
Extension of the rice DH population genetic map with microsatellite markers
Yunbi Xu,Lishuang Shen,Susan R. McCouch,Lihuang Zhu
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02883931
Abstract: Genetic mapping of microsatellite markers was carried out in a rice DH population derived from across between Zaiyeqing 8 (indica) and Jingxi 17 (japonica). A total of 89 microsatellite markers, including 84 (GA)n, 2(TCT)n, 2(ATT)n, and, l(ATC)n motifs, were integrated relatively evenly into the established genetic map of the DH population. This will facilitate the utilization of microsatillite markers in rice gene mapping and marker aided breeding.
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