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Search Results: 1 - 10 of 666023 matches for " Lima J. Albérsio A. "
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Production of policlonal antisera specific to plant viruses by rabbit oral immunization
LIMA, J. ALBéRSIO A.;LIMA, ROBERTO C.A.;GON?ALVES, M. FáTIMA B.;
Fitopatologia Brasileira , 2001, DOI: 10.1590/S0100-41582001000400016
Abstract: serological techniques are of great importance for plant virus identification and characterization. the major limiting factor for using these techniques for plant virus identification is the requirement of a good virus purified preparation to be used in immunizing animals for antiserum production. in the present study, two new zealand rabbits were orally immunized with extracts from cowpea (vigna unguiculata) plants systemically infected with cowpea severe mosaic virus (cpsmv) and with extracts from papaya (carica papaya) infected with papaya lethal yellowing virus (plyv). the leaf extracts were prepared in saline solution 0.15 m in the rate of 1:1 (w/v) and clarified by a centrifugation of 10,000 g for 10 min. the clarified extracts containing the viruses were orally administered to the new zealand rabbits in two series of five daily doses of 1.0 ml each. the obtained policlonal antisera were shown to be very specific to their respective viruses in double immunodiffusion and indirect elisa. these seem to be the first antisera specific for plant virus obtained by rabbit oral immunization. the results open up some possibilities for producing antisera to plant viruses of difficult purification. it is a simple, fast and inexpensive method for production of antisera for plant viruses when compared to the traditional techniques that involve rabbit injections with purified virus preparations.
Production of policlonal antisera specific to plant viruses by rabbit oral immunization
LIMA J. ALBéRSIO A.,LIMA ROBERTO C.A.,GON?ALVES M. FáTIMA B.
Fitopatologia Brasileira , 2001,
Abstract: Serological techniques are of great importance for plant virus identification and characterization. The major limiting factor for using these techniques for plant virus identification is the requirement of a good virus purified preparation to be used in immunizing animals for antiserum production. In the present study, two New Zealand rabbits were orally immunized with extracts from cowpea (Vigna unguiculata) plants systemically infected with Cowpea severe mosaic virus (CPSMV) and with extracts from papaya (Carica papaya) infected with Papaya lethal yellowing virus (PLYV). The leaf extracts were prepared in saline solution 0.15 M in the rate of 1:1 (w/v) and clarified by a centrifugation of 10,000 g for 10 min. The clarified extracts containing the viruses were orally administered to the New Zealand rabbits in two series of five daily doses of 1.0 ml each. The obtained policlonal antisera were shown to be very specific to their respective viruses in double immunodiffusion and indirect ELISA. These seem to be the first antisera specific for plant virus obtained by rabbit oral immunization. The results open up some possibilities for producing antisera to plant viruses of difficult purification. It is a simple, fast and inexpensive method for production of antisera for plant viruses when compared to the traditional techniques that involve rabbit injections with purified virus preparations.
Efeitos da intera??o de potyvirus em híbridos de meloeiro, variedades de melancia e abobrinha
Ramos, Najara F.;Lima, J. Albérsio A.;Gon?alves, M. Fátima B.;
Fitopatologia Brasileira , 2003, DOI: 10.1590/S0100-41582003000200016
Abstract: considering the frequent natural occurrence of mixed infection of two or more viruses in the same plant, the present research was developed with the objective of studying the symptomatological effects of mixed virus infections in melon (cucumis melo), watermelon (citrullus lanatus) and zucchini (cucurbita pepo). the following virus species from the family potyviridae genus potyvirus were used in the study: papaya ringspot virus (prsv), watermelon mosaic virus (wmv), and zucchini yellow mosaic virus (zymv). the symptomatological effects were evaluated in double and single infections in three melon hybrids hy mark, gold mine and orange flesh, two watermelon varieties crimson sweet and charleston gray and zucchini 'caserta'. in greenhouse experiments, prsv, wmv and zymv alone and in all possible double combinations were inoculated in ten-day-old melon, watermelon and zucchini plants, using ten plants of each hybrid or variety per virus combination. inoculations were performed using leaf extracts from plants infected with each virus. leaf samples from each virus combination were tested by elisa against antisera specific for the respective viruses. the symptomatological effects in double virus infections revealed the existence of synergistic interactions between prsv, wmv and zymv in melon, watermelon and zucchini. the double infections involving zymv showed severe symptoms and symptoms not present in zymv single infection.
Biological and physicochemical properties of cowpea severe mosaic comovirus isolated from soybean in the State of Paraná
Bertacini, Paula V.;Almeida, álvaro M.R.;Lima, J. Albérsio A.;Chagas, Cesar M.;
Brazilian Archives of Biology and Technology , 1998, DOI: 10.1590/S1516-89131998000400004
Abstract: soybean plants with symptoms of bud blight were growing close to cowpea with severe symptoms of mosaic associated with blisters in the leaves. a group of plants of both species were collected and used for etiological studies. this kind of symptom in soybeans was common in certain areas of the state of paraná, induced by tobacco streak ilarvirus. host range, serological reaction, particle morphology and size, protein and nucleic acid analysis, and transmission by beetles from species cerotoma arcuata oliv. showed that the virus involved was cowpea severe mosaic comovirus. this is the first report on the occurrence of this virus in soybean plants in the state of paraná. results using indirect elisa showed that in cowpea the relative virus concentration was higher in green leaf areas than in chlorotic ones. also, virus concentration, determined through indirect elisa was much higher in plants kept at diurnal regime of 25o c x 23o c (12 x 12 h) than at 30o c x 28o c.
Efeitos da intera o de potyvirus em híbridos de meloeiro, variedades de melancia e abobrinha
Ramos Najara F.,Lima J. Albérsio A.,Gon?alves M. Fátima B.
Fitopatologia Brasileira , 2003,
Abstract: Em raz o da freqüente ocorrência de infec o mista, na natureza, o presente trabalho objetivou estudar o efeito da intera o de diferentes espécies de potyvírus em meloeiro (Cucumis melo), melancia (Citrullus lanatus) e abobrinha (Cucurbita pepo). Foram usados os seguintes vírus da família Potyviridae, gênero Potyvirus: Papaya ringspot virus (PRSV); Watermelon mosaic virus, (WMV) e Zucchini yellow mosaic virus, (ZYMV). Os efeitos na sintomatologia das infec es duplas e simples de PRSV, WMV e ZYMV foram avaliados em três híbridos de meloeiro, duas variedades de melancia e abobrinha 'Caserta', em experimentos de casa de vegeta o. Os três vírus, isoladamente ou em todas as duplas combina es possíveis, foram inoculados, em plantas dos híbridos de meloeiro Hy Mark, Gold Mine e Orange Flesh, variedades de melancia Crimson Sweet e Charleston Gray e abobrinha 'Caserta', usando-se dez plantas de cada híbrido ou variedade, por combina o de vírus. As inocula es foram efetuadas por meio de extratos de folhas com infec o simples dos respectivos vírus. As plantas inoculadas com cada vírus isoladamente e suas respectivas combina es foram observadas quanto ao aparecimento de sintomas durante 30 dias após as inocula es. Amostras foliares das plantas inoculadas foram, também, testadas por ELISA indireto contra os anti-soros correspondentes para cada vírus. As infec es duplas em meloeiro, melancia e abobrinha revelaram, através da avalia o sintomatológica, que existem intera es sinérgicas entre PRSV, WMV e ZYMV. As infec es duplas envolvendo o ZYMV apresentaram alta severidade, exibindo sintomas n o encontrados em infec es simples, apesar da severidade nas infec es isoladas do ZYMV.
Transmiss?o por m?os contaminadas e ausência de transmiss?o embrionária do vírus do amarelo letal do mamoeiro
Saraiva, Armando C. M.;Paiva, Waldelice O. de;Rabelo Filho, Francisco A. C.;Lima, J. Albérsio A.;
Fitopatologia Brasileira , 2006, DOI: 10.1590/S0100-41582006000100014
Abstract: the "papaya lethal yellowing virus" (plyv) is responsible for an important disease of papaya (carica papaya) in northeast brazil. plyv can be transmitted by soil, water and contaminated cutting instruments, as well as through mechanical inoculation. the objective of the present work was to evaluate the possibility of the virus transmission by contaminated hands, and its presence in the embryos of infected papaya fruit seeds. the virus was transmitted through contaminated hands, thus revealing that plyv is very stable. experiments with embryos from 1,128 seeds of infected fruits revealed the absence of plyv in the embryos from seeds of infected fruits, although the virus was present in 112 out of the 670 seed peel samples analyzed. direct planting of seeds from infected fruits, involving 310 seedlings and 362 plant-seedlings, did not perpetuate plyv, demonstrating that the virus is not transmitted in the embryo of the seeds. on the other hand, plyv was detected by indirect elisa in 49 out of 456 seeds per si.
Transmiss o por m os contaminadas e ausência de transmiss o embrionária do vírus do amarelo letal do mamoeiro
Saraiva Armando C. M.,Paiva Waldelice O. de,Rabelo Filho Francisco A. C.,Lima J. Albérsio A.
Fitopatologia Brasileira , 2006,
Abstract: O vírus do amarelo letal do mamoeiro ("Papaya lethal yellowing virus", PLYV) é responsável por uma das principais doen as do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido por solo, água, instrumentos de corte contaminados e por inocula o mecanica em condi es experimentais. Na presente pesquisa, avaliou-se a transmiss o do PLYV por m os contaminadas e sua presen a em embri es de sementes de frutos infetados. O PLYV foi transmitido por m os contaminadas, e m os contaminadas e lavadas em água corrente revelando elevada estabilidade. Experimentos com 1.128 embri es de sementes obtidas de frutos infetados com o vírus revelaram a ausência do PLYV, cuja presen a foi constatada em 112 tegumentos das 670 sementes analisadas. O PLYV n o foi perpetuado por plantio direto de sementes, envolvendo 310 plantulas e 362 mudas, comprovando n o ser o mesmo transmitido por semente de forma embrionária. De outra parte, a presen a do PLYV foi comprovada por Elisa indireto em 49 de 456 sementes per si testadas.
Sintomas atípicos em frutos de meloeiro e de melancia ocasionados por Watermelon mosaic virus-2
LIMA, J. ALBéRSIO A.;QUEIROZ, M. ABíLIO DE;RAMOS, NAJARA F.;GON?ALVES, M. FáTIMA B.;
Fitopatologia Brasileira , 2002, DOI: 10.1590/S0100-41582002000500021
Abstract: the northeast of brazil presents great potencial for melon (cucumis melo) and watermelon (citrullus lanatus) production. melon fruit with white spots and watermelon fruits showing chlorotic spots were demonstrated to be caused by watermelon mosaic virus-2, through indirect enzyme linked-immuno sorbent assay.
Sintomas atípicos em frutos de meloeiro e de melancia ocasionados por Watermelon mosaic virus-2
LIMA J. ALBéRSIO A.,QUEIROZ M. ABíLIO DE,RAMOS NAJARA F.,GON?ALVES M. FáTIMA B.
Fitopatologia Brasileira , 2002,
Abstract: The Northeast of Brazil presents great potencial for melon (Cucumis melo) and watermelon (Citrullus lanatus) production. Melon fruit with white spots and watermelon fruits showing chlorotic spots were demonstrated to be caused by Watermelon mosaic virus-2, through indirect enzyme linked-immuno sorbent assay.
Sele??o de acessos e progênies de Citrullus spp. para resistência a três potyvirus
Silveira, Lindomar M. da;Queiróz, Manoel A. de;Lima, J. Albérsio de A.;Negreiros, Maria Z. de;Ramos, Najara F.;Nascimento, Aline K. Q. do;
Fitopatologia Brasileira , 2005, DOI: 10.1590/S0100-41582005000400009
Abstract: six watermelon (citrullus spp.) accessions (87-019, 87-029, 91-080, pi-244018, 91-043 and pi-195927) and two samples (pi-244019a and pi-244019b) from the acession pi-244019b from embrapa cucurbit germplasm bank in petrolina-pe, northeast of brazil were evaluated in an attempt to select accessions and progenies of watermelon as sources of resistance to papaya ringspot virus type watermelon (prsv-w), watermelon mosaic virus (wmv) and zucchini yellow mosaic virus (zymv). endogamic and open pollinated progenies from these accessions were also evaluated for resistance to prsv-w, wmv and zymv. the evaluations were carried out in a greenhouse at the plant virus laboratory of the federal university of ceará where the plants were mechanically inoculated and serological screened by elisa. the selected plants were grown in the experimental station of bebedouro in petrolina-pe, where the virus inoculations were made, naturally, by vectors. according to the results, plants were not infected by prsv-w in the following accessions: 87-019, pi-244019a, 91-080, pi-244018, pi-244019b and pi-195927; plants were not infected by wmv in the accessions: 87-019 and 87-029; and plants were not infected by zymv in the accessions: pi-244019a, 87-029, 91-080,91-043, pi-244019b and pi-195927. the progenies showed differentiated behavior with the percentage of selected plants ranging from 20 to 100% for prsv-w and 60 to 100% for wmv. the progenies evaluated against zymv did not show resistant plants. therefore, it can be postulated that resistance for prsv-w and wmv in the progenies differs from that for zymv, since all progenies were submitted to the same number of self-pollinations.
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