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Search Results: 1 - 10 of 23105 matches for " Liang Gaofeng "
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Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots
Liang Gaofeng,Ma Chao,Zhu Yanliang,Li Shuchun
Nanoscale Research Letters , 2011,
Abstract: Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the performance of multi-PCR by reducing the formation of nonspecific products in the complex system, but an excessive amount of CdTe QDs could suppress the PCR. The effects of QDs on PCR can be reversed by increasing the polymerase concentration or by adding bovine serum albumin (BSA). The mechanisms underlying these effects were also discussed. The results indicated that CdTe QDs could be used to optimize the amplification products of the PCR, especially in the multi-PCR system with different primers annealing temperatures, which is of great significance for molecular diagnosis.
The correlation between magnetic resonance imaging features of the brainstem and cerebellum and clinical features of spinocerebellar ataxia 3/Machado-Joseph disease
Liang Xiaochun,Jiang Hong,Chen Changqing,Zhou Gaofeng
Neurology India , 2009,
Abstract: Background : Brainstem and cerebellar atrophy are the most important features in magnetic resonance imaging (MRI) in spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD). However, the correlation between brainstem and cerebellar atrophy and the clinical features has not been well studied. Aim : To study the correlation between MRI features of the brainstem and cerebellum and predominant clinical features in SCA3/MJD. Design and Setting : University teaching hospital. Patients and Methods : By using the linear measurement method, we assessed 32 patients with SCA3/MJD to study the correlations between the morphometric data of the brainstem and cerebellum and clinical features: Duration of the disease, age of onset, total international cooperative ataxia rating scale (ICARS) score; total scale for the assessment and rating of ataxia (SARA) score; ICARS subscores, and SARA subscores. Statistical Analysis : Pearson correlation test. Results : There was a significant invese correlation between anteroposterior diameter of the midbrain and pons and total ICARS scores, total SARA scores, ICARS and SARA subscores (r= -0.381~-0.57, P < 0.05 or 0.01) and disease duration (r=- 0.42~-0.51, P < 0.05 or 0.01). Additionally, superoinferior diameter of the cerebellum was inversely correlated with total SARA scores and ICARS and SARA subscores except for ataxia of posture and gait in both scales (r=- 0.37~-0.44, P < 0.05). The superoinferior diameter of the fourth ventricle was inversely correlated with age of onset (r=-0.45, P < 0.05). Conclusion : The effect on the cerebellum and brainstem is related to predominant clinical features in SCA3/MJD patients.
Study on the Law of the Movement and Damage to Slope with the Combination of Underground Mining and Open-Pit Mining  [PDF]
Gaofeng Ren, Xingkui Fang
Engineering (ENG) , 2010, DOI: 10.4236/eng.2010.23029
Abstract: Under circumstances in which both underground mining and open-pit mining are employed, the mining effects of two approaches will be superposed and the mining slope will receive several induced stress fields, which makes the sliding mechanism and deformation law of slope rock mass more complicated. This paper, targeting at the east slope of Antaibao Mine with the joint employment of underground mining and open-pit mining, aims to study the moving law of the slope rock mass and the damage mechanism to the overburden of the goaf by numerical simulation. It is supposed that models of possible damage to the slope could be explored for guidance to safety-production of the mine.
Levels of synthetic musk fragrances in human milk from three cities in the Yangtze River Delta in Eastern China

Zhang Xiaolan,Liang Gaofeng,Zeng Xiangying,Zhou Jing,Sheng Guoying,Ful Jiamo,

环境科学学报(英文版) , 2011,
Abstract: Synthetic musks are used as additives in many household products. After absorption into the human body, they accumulate and their concentrations in human milk reflect both the mother and her infant's exposure level. Concentrations of four synthetic musks, musk xylene (1-tert-butyl-2,6-dimethyl-2,4,6-trinitrobenzene, MX), musk ketone (4-tert-butyl-2,6-dimethyl-3,5-dinitroacetophenone, MK), 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopentagamma]-2-benzopyran (HHCB) and 7-acetyl-1,1,3,4,4,6-hexamethyl- 1,2,3,4-tetrahydronaphthalene (AHTN), were determined in human milk samples collected from Shanghai, Wuxi, and Shaoxing in Eastern China. The four synthetic musks were found in most samples analyzed, with HHCB the dominant component followed by MX. The median (mean) values for HHCB, AHTN, MX and MK concentrations were 63 (82), 5 (12), 17 (24) and 4 (9) ng/g lipid weight, respectively. These data suggested the total synthetic musk contamination was low, and the distribution percentage was HHCB > MX > AHTN approximately MK. The relative high ratio of nitro to polycylic musk indicated that nitro musks were still widely used. The musk concentrations in these cities were not significantly different from each other (p > 0.05). Principal components score plots were obtained, which showed similar exposure sources. The amount of total synthetic musks in human milk were not associated with mother's age, although HHCB was significantly correlated with AHTN (p < 0.05). Daily ingestion of HHCB, AHTN, MX and MK for infants from human milk were estimated as (2526 +/- 2926), (370 +/- 524), (7391 +/- 832), and (277 +/- 462) ng/day, respectively. Those doses were 1-2 orders of magnitude below the provisional tolerable daily intakes.
Bifurcation Analysis of an SIR Epidemic Model with the Contact Transmission Function
Guihua Li,Gaofeng Li
Abstract and Applied Analysis , 2014, DOI: 10.1155/2014/930541
Abstract: We consider an SIR endemic model in which the contact transmission function is related to the number of infected population. By theoretical analysis, it is shown that the model exhibits the bistability and undergoes saddle-node bifurcation, the Hopf bifurcation, and the Bogdanov-Takens bifurcation. Furthermore, we find that the threshold value of disease spreading will be increased, when the half-saturation coefficient is more than zero, which means that it is an effective intervention policy adopted for disease spreading. However, when the endemic equilibria exist, we find that the disease can be controlled as long as we let the initial values lie in the certain range by intervention policy. This will provide a theoretical basis for the prevention and control of disease. 1. Introduction The classical SIR model for disease transmission has been widely studied. It is one of the most important issues that the dynamical behaviors are changed by the different incidence rate in epidemic system. For the incidence rate, we divided into two categories: one is that Capasso and Serio [1] proposed the infection force which is a saturated curve, described “crowding effect” or “protection measures;” the other is the infection force that describes the effect of “intervention policy,” for example, closing schools and restaurants and postponing conferences (see Figure 1). For the model with the saturated infection force, , which is one of the typical infection forces, the rich dynamical behaviors were founded by Ruan and Wang [2] and Tang et al. [3]. The model with the incidence rate can be suited for many infectious diseases, including measles, mumps, rubella, chickenpox, and influenza. For more research literatures about nonlinear infection rate see [4–9]. However, for some parasite-host models, by observing macro- and microparasitic infections, one finds that the infection rate is an increasing function of the parasite dose, usually sigmoidal in shape [10, 11]. So we will build a model with sigmoidal incidence rate which is taken into account “crowding effect” and “saturated effect.” According to the parasite-host model which is proposed by Anderson and May (1979) [12, 13], the model is as follows: where are susceptible hosts, infected hosts, and removed hosts, respectively. is the birth rate of susceptible host, is the natural death rate of a population, is the removal rate, and is the per capita infection-related death rate. If we denote infection force , can be explained as the rate of valid contact. At the beginning of disease, most people have poor awareness of
Anisotropic Scattering for a Magnetized Cold Plasma Sphere  [PDF]
Yingle Li, Mingjun Wang, Qunfeng Dong, Gaofeng Tang
Energy and Power Engineering (EPE) , 2010, DOI: 10.4236/epe.2010.22017
Abstract: The transformation of parameter tensors for anisotropic medium in different coordinate systems is derived. The electric field for a magnetized cold plasma sphere and the general expression of scattering field from anisotropic target are obtained. The functional relations of differential scattering cross section and the RCS for the magnetized plasma sphere are presented. Simulation results are in agree with that in the literatures, which shows the method used and results obtained are correct and the results provide a theoretical base for anisotropic target identification etc.
Performance Improvement of Polyvinyl Formal Based Gel Polymer Electrolyte for Lithium-Ion Batteries by Coating Al2O3  [PDF]
Yan Wen, Gaofeng Li, Pingping Zhang, Guohong Xiong
Journal of Applied Mathematics and Physics (JAMP) , 2016, DOI: 10.4236/jamp.2016.42023
Abstract:

Al2O3/PVFM/Al2O3 trilayer membranes are prepared by means of simple coating of PVA-Al2O3 solution onto both sides of PVFM thin membranes, which is prepared via phase inversion method. The characteristics of the trilayer membranes and gel polymer electrolytes are investigated using FESEM, tensile testing apparatus, thermal shrinkage test, EIS and charge-discharge test. When inorganic Al2O3 particles are used to coat the PVFM membrane, drawbacks associated with gel-type membranes, namely, poor mechanical strength and thermal stability are greatly improved. Lithium ion cell with the Al2O3/PVFM/Al2O3 based GPE matched with LiFePO4 shows excellent electrochemical performance.

The modernization of scientific and technical journal and editorial standardization
科技期刊现代化与编辑规范化

Yuwen Gaofeng,
宇文高峰

中国科技期刊研究 , 2000,
Abstract: 依据现代科技期刊发展,分析了科技期刊现代化与编辑规范化的演变及其之间关系,提出了实现科技期刊现代化和规范化的途径和措施。
BEC-BCS Crossover in the Nambu--Jona-Lasinio Model of QCD
Sun, Gaofeng;He, Lianyi;Zhuang, Pengfei
High Energy Physics - Phenomenology , 2007, DOI: 10.1103/PhysRevD.75.096004
Abstract: The BEC-BCS crossover in QCD at finite baryon and isospin chemical potentials is investigated in the Nambu--Jona-Lasinio model. The diquark condensation in two color QCD and the pion condensation in real QCD would undergo a BEC-BCS crossover when the corresponding chemical potential increases. We determined the crossover chemical potential as well as the BEC and BCS regions. The crossover is not triggered by increasing the strength of attractive interaction among quarks but driven by changing the charge density. The chiral symmetry restoration at finite temperature and density plays an important role in the BEC-BCS crossover. For real QCD, strong couplings in diquark and vector meson channels can induce a diquark BEC-BCS crossover in color superconductor, and in the BEC region the chromomagnetic instability is fully cured and the ground state is a uniform phase.
The Size Distribution of Chinese Manufacturing Firms: From the Perspective of Industry Life Cycle
Yanying CHEN,Li WANG,Gaofeng TIAN
International Journal of Economics and Finance , 2012, DOI: 10.5539/ijef.v4n7p67
Abstract: Using panel data of Chinese manufacturing firms between 2003 and 2008, this paper aims to examine the evolution of firm size distribution (FSD) as the industry goes through its life-cycle. The results reveal that during the life-cycle process, China’s manufacturing firms’ average size becomes larger then shrinks, and the degree of firm size heterogeneity and industry concentration increases all the time. Empirical results also indicate that the change rate of FSD is affected by firms’ net entry rate into industry. When net entry rate is higher, average firm size’s growth rate is smaller, while size heterogeneity and industry concentration rises more dramatically.
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