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Search Results: 1 - 10 of 3765 matches for " LiJia Qu "
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Methylation of phytohormones by the SABATH methyltransferases
LiJia Qu,Shuang Li,ShuFan Xing
Chinese Science Bulletin , 2010, DOI: 10.1007/s11434-010-3245-x
Abstract: In plants, one of the most common modifications of secondary metabolites is methylation catalyzed by various methyltransferases. Recently, a new class of methyltransferases, the SABATH family of methyltransferases, was found to modify phytohormones and other small molecules. The SABATH methyltransferases share little sequence similarity with other well characterized methyltransferases. Arabidopsis has 24 members of the SABATH methyltransferase genes, and a subset of them has been shown to catalyze the formation of methyl esters with phytohormones and other small molecules. Physiological and genetic analyses show that methylation of phytohormones plays important roles in regulating various biological processes in plants, including stress responses, leaf development, and seed maturation/germination. In this review, we focus on phytohormone methylation by the SABATH family methyltransferases and the implication of these modifications in plant development.
Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis
Jinling Wang,Lijia Qu,Jun Chen,Hongya Gu,Zhangliang Chen
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02886256
Abstract: The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution. In this study, three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis, Ginkgo biloba) and seven from four species in angiosperm (Magnolia denudata, Salix babylonica, Nymphaea tetragona, Camellia japonica) have been amplified by PCR from genomic DNA and sequenced. Together with other 73 sequences ofCHS collected from EMBL database and literature, these sequences, which embrace 19 families of gymnosperm and angiosperm, have been analyzed for their phylogenetic relations by parsimony method. The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae, Magnoliaceae and Nymphaeaceae. The relative rate test revealed the rate heterogeneity of CHS genes among the families. For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae, Asteraceae and Solanaceae evolve faster for the nonsynonymous substitution. These results suggest that the duplication and extinction events of CHS genes are different among systematic families, therefore it seems impractical to look for orthologous sequences from CHS genes to study plant phylogeny at the family level andlor above. However, it is possible to do so below the family level.
Obtaining transgenic rice resistant to rice fungal blast disease by controlled cell death strategy
Shengji Mao,Hongya Gu,Lijia Qu,Zhangliang Chen
Chinese Science Bulletin , 2003, DOI: 10.1360/03wc0049
Abstract: The strategy of the two-component system, composed of Barnase and Barstar which encode RNase and a specific inhibitor to the RNase respectively, is adopted to obtain transgenic rice resistant to rice fungal blast disease. In this study, two chimeric promoters, induced by rice blast fungus pathogen (Magnaporthe grisea), are fused with Barnase respectively to construct two plant expression vectors, pWBNBS and pPBNBS together with the Barstar driven by CaMV 35S promoter. The resistance of the transgenic rice lines to rice blast fungus disease and rice blight disease are evaluated. The results show that (1) the expression of Barnase is induced in rice leaves when inoculated with the spores of Magnaporthe grisea; (2) the induced expression level of Barnase surpasses the level of Barstar, which elicits a similar hypersensitive response (HR) in the leaves, and the transgenic plant shows high resistance to the rice fungal blast disease; and (3) transgenic rice plants also show obvious resistance to rice bacterial blight disease. Taken together, these results suggest that the transgenic rice plants harboring this two-component system acquire relatively broad spectrum resistance against pathogens, especially high resistance to rice fungal pathogen.
Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)
Lijia Qu,Donghui Li,Yi Zhang,Meihua Liu,Hongya Gu,Zhangliang Chen
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02884667
Abstract: A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80s ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this riceS4 gene is from a multigene family.
Functional analysis of the expressed product of a rice allergen cDNA inE. coli
Jianhua Jin,Houbin Zhang,Lijia Qu,Ming Xie,Hongya Gu,Zhangliang Chen
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02883940
Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice
Huafei Lü,Xiaotian Ming,Lijia Qu,Meihua Liu,Jing Li,Hongya Gu,Zhangliang Chen
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02884682
Abstract: The promoter fragments of wheatGstA1 and potatoGst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5′ untranslated leader sequence together withGUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors ofPyricularia oryzae in transgenic rice cells; the intron I of riceAct1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and theAct1 minimal promoter and its 5′ untranslated leader sequence produced low level background expression in rice.
Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress
Fei He,JiuQing Kang,Xin Zhou,Zhen Su,LiJia Qu,HongYa Gu
Chinese Science Bulletin , 2008, DOI: 10.1007/s11434-008-0403-5
Abstract: The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up-or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key transcription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China.
Inducible expression pattern of rice Bowman-Birk inhibitor geneOsWIP1-2 and its protease inhibitory activity
Jun Chen,Jing Liu,Lei Guo,Lijia Qu,Zhangliang Chen,Hongya Gu
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03184006
Abstract: TheWIP1-2 gene was cloned from rice. It belongs to the Bowman-Birk inhibitor gene family. Northern blot showed that expression of this gene was induced by wounding and jasmonic acid (JA). It indicates that theOsWIP1 gene plays an important role in the rice defense system. TheOsWIP1-2 was cloned into pET28a and expressed inE. coli. Its expressed product was purified in the form of fusion protein and tested for the inhibitory activities against trypsin and chymotrypsin. It was found that the fusion protein could inhibit chymotrypsin, but not trypsin. It was also found that the His tag at its C-terminal affected its inhibitory activity significantly. The fusion protein with a natural C-terminal had the inhibitory activity, while no inhibitory activity was detected in the fusion protein with a (His)6-tag at its C-terminal. This implies that extra amino acid residues at the C-terminal of OsWIP1-2 may interfere with its correct folding. The inhibitory assay indicated that the members of rice Bowman-Birk inhibitor gene family probably differentiated both in their structure and function.
Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens
Yi Zhang,Lijia Qu,Meihua Liu,Xiaoli Qin,Hongya Gu,Zhangliang Chen
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02898956
Abstract: The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in undestanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysised, showing high structural similarity with two other anther-specific genes (A1 and BA42) fromBrassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development.
Regulation of Leaf Development by Auxin in Arabidopsis

Linchuan Li,Lijia Qu,

植物学报 , 2006,
Abstract: Leaf is the first lateral organ produced by the activity of the shoot apical meristem (SAM). Early leaf development is artificially divided into three main stages: the initiation of leaf primordium, the establishment of leaf adaxial-abaxial polarity, and the expansion of leaf blade. Numerous of evidence indicates that leaf development is regulated by inner genetic mechanism and outer environmental cues. Phytohormones, especially auxin, play essential roles in coordination of these two regulation mechanisms. Auxin homeostasis, polar transport and signaling affect the whole progress of leaf development. In this mini-review, we will summarize recent progress of auxin regulation in leaf development and morphogenesis, and try to understand the complex regulation network of leaf development.
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