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Search Results: 1 - 10 of 127045 matches for " Li Tianzhen "
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Contour Extraction of Skin Tumors Using Visual Attention and GVF-Snake Model  [PDF]
Li Ma, Tianzhen Su
Engineering (ENG) , 2013, DOI: 10.4236/eng.2013.510B099
Abstract:

Contour extraction of skin tumors accurately is an important task for further feature generation of their borders and sur-faces to early diagnose melanomas. An integrated approach, combining visual attention model and GVF-snake, is pro-posed in the paper to provide a general framework for locating tumor boundaries in case of noise and boundaries with large concavity. For any skin image, the visual attention model is implemented to locate the Region of Interests (ROIs) based on saliency maps. Then an algorithm called GVF-snake is utilized to iteratively drive an initial contour, deriving from the extracted ROIs, towards real boundary of skin tumors by minimizing an energy function. It is shown from ex-periments that the proposed approach exceeds in two aspects compared with other contour-deforming methods: 1) ini-tial contours generated from saliency maps are definitely located at neighboring regions of real boundaries of skin tu-mors to speed up converges of contour deformation and achieve higher accuracy; 2) the method is not sensitive to nois-es on skins and initial contours extracted.

Transcriptomic Analysis of Fiber Strength in Upland Cotton Chromosome Introgression Lines Carrying Different Gossypium barbadense Chromosomal Segments
Lei Fang, Ruiping Tian, Jiedan Chen, Sen Wang, Xinghe Li, Peng Wang, Tianzhen Zhang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094642
Abstract: Fiber strength is the key trait that determines fiber quality in cotton, and it is closely related to secondary cell wall synthesis. To understand the mechanism underlying fiber strength, we compared fiber transcriptomes from different G. barbadense chromosome introgression lines (CSILs) that had higher fiber strengths than their recipient, G. hirsutum acc. TM-1. A total of 18,288 differentially expressed genes (DEGs) were detected between CSIL-35431 and CSIL-31010, two CSILs with stronger fiber and TM-1 during secondary cell wall synthesis. Functional classification and enrichment analysis revealed that these DEGs were enriched for secondary cell wall biogenesis, glucuronoxylan biosynthesis, cellulose biosynthesis, sugar-mediated signaling pathways, and fatty acid biosynthesis. Pathway analysis showed that these DEGs participated in starch and sucrose metabolism (328 genes), glycolysis/gluconeogenesis (122 genes), phenylpropanoid biosynthesis (101 genes), and oxidative phosphorylation (87 genes), etc. Moreover, the expression of MYB- and NAC-type transcription factor genes were also dramatically different between the CSILs and TM-1. Being different to those of CSIL-31134, CSIL-35431 and CSIL-31010, there were many genes for fatty acid degradation and biosynthesis, and also for carbohydrate metabolism that were down-regulated in CSIL-35368. Metabolic pathway analysis in the CSILs showed that different pathways were changed, and some changes at the same developmental stage in some pathways. Our results extended our understanding that carbonhydrate metabolic pathway and secondary cell wall biosynthesis can affect the fiber strength and suggested more genes and/or pathways be related to complex fiber strength formation process.
Volatility Analysis of Web News and Public Attitude by GARCH Model  [PDF]
Pinrui Yu, Tianzhen Liu, Qian Ding
Psychology (PSYCH) , 2012, DOI: 10.4236/psych.2012.38092
Abstract: GARCH (Generalized Auto-Regressive Conditional Heteroskedasticity) model proposed by Professor Engle is successful to analyze the volatility of stock price. In this paper GARCH model is used to analyze the volatility of web news events and public attitudes by the data coming from typical news events in famous web. The results show that the volatility of web news events and public attitudes are suitable to GARCH model by some adjusting and test of parameters.
Commercial production of transgenic Bt insect-resistant cotton varieties and the resistance management for bollworm (Helicoverpa armigera Hubner)
Tianzhen Zhang,Canming Tang
Chinese Science Bulletin , 2000, DOI: 10.1007/BF03182897
Abstract: There are currently three kinds of transgenic Bt insect-resistant cotton gerrnplasm lines, Shanxi 94-24, Zhongxin 94 and R19, in China. They showed high resistance to the neonate larvae of bollworm (Helicoverpa armigera). Transgenic Bt insect-resistant cotton varieties or hybrids have been bred using the three kinds of germplasm lines as parents. Our researches reveal that there exist different expressions in resistant level at different developmental stages in the three categories of germplasm lines. When neonate larvae are fed with leaves of cotton plant at the seeding stage with less than 10 leaves on the main stem, the mortality of the neonate larvae is 100%, but the resistance level will decline at later season. When Bt gene has been transferred to the cotton genome, it can be steadily transferred to the progeny, the level of resistance to bollworm keeps fundamentally uniform. Such insects as tobacco budworm (Heliothis virencens) in laboratory directive selection are very apt to produce resistance to the Bt insecticidal crystal protein. From the present crop system of cotton region in the Yangtze and Yellow River Valleys, and the expression characteristic of transgenic Bt resistant cotton, we suggest that the resistance to toxin protein in bollworm is not apt to be produced if the transgenic Bt insect-resistant cotton varieties are released and grown in the regions except in the Xinjiang cotton region. The managing strategies to delay or retard the resistance are discussed.
Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells
Tianzhen Wang, Ran Zhao, Yiqi Wu, Dan Kong, Lei Zhang, Di Wu, Chao Li, Chong Zhang, Zuxi Yu, Xiaoming Jin
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-231
Abstract: MTT, colony formation assay and tumourigenicity in nude mice were performed to investigate the effect of HBV on the proliferative capability of host cells. In order to explore the potential mechanism, cell cycle and apoptosis were analysed. The cell cycle genes controlling the G1/S phase transition were detected by immunohistochemistry, westernblot and RT-PCR.HepG2.2.15 cells showed decreased proliferation ability compared to HepG2 cells. G1 phase arrest was the main cause but was not associated with apoptosis. p53, p21 and total retinoblastoma (Rb) were determined to be up-regulated, whereas cyclinE was down-regulated at both the protein and mRNA levels in HepG2.2.15 cells. The phosphorylated Rb in HepG2.2.15 cells was decreased.Our results suggested that HBV inhibited the capability of proliferation of HepG2.2.15 cells by regulating cell cycle genes expression and inducing G1 arrest.Epidemiological and virological investigations have shown that hepatitis B virus (HBV) infection is the main cause of hepatocellular carcinoma (HCC) and is present in approximately 80% of HCC patients [1-4]. Although considerable studies have been done, the precise mechanism remains unclear.By now, HBV genome integration, gene mutation, gene deletion and diverse viral factors have been proved to be implicated in HBV-related HCC [5-8]. However, little is known about the impacts of the complete HBV genome or HBV replication on host cells.The HepG2.2.15 cell line was established by transfecting the HBV genome into HepG2 cells [9]. It supports stable HBV replication and protein expression, as well as the production of virus particles. HepG2.2.15 is a widely used cell line in the study of the life cycle of HBV and antiviral research [10-12]. It is also an ideal model for investigating host-virus interaction [13,14]. Our previous study has found that HepG2.2.15 cell line demonstrated distinct biological features compared with parental HepG2. The comparative analysis between HepG2.2.15 and He
Modified fiber qualities of the transgenic cotton expressing a silkworm fibroin gene
FeiFei Li,ShenJie Wu,FenNi Lü,TianZi Chen,Ming Ju,HaiHai Wang,YanJie Jiang,Jie Zhang,WangZhen Guo,TianZhen Zhang
Chinese Science Bulletin , 2009, DOI: 10.1007/s11434-009-0142-2
Abstract: A silkworm gene for fibroin was introduced into the upland cotton WC line by Agrobacterium-mediated transformation. PCR detection for fibroin, nptII and gus genes, Kanamycin (Km)-resistance analysis and GUS-histochemical assay were conducted on 30 regenerated plants from 9 callus lines, and 17 positive plants were obtained by these 5 screening methods. By Km-resistance analysis and PCR for fibroin, 6 homozygous lines in T3 were obtained. Southern blot and Northern bolt demonstrated that the fibroin gene was inserted into the genome of these 6 lines, stably inherited and expressed. Compared to the control, the surface structure of mature fiber in the 6 lines was significantly distorted and an increased number of convolution was observed by scanning electron microscopy (SEM). Fiber quality traits analysis indicated that fiber elongation of the 6 homozygous lines was all increased and fiber strength of 3 lines was enhanced. These results indicated that fibroin expression influenced cotton fiber structure and quality, suggesting that fibroin has great potential for improving cotton fiber quality by genetic engineering.
Would Rural Residents Will to Pay for Environmental Project? An Evidence in China  [PDF]
Xuyin Zhang, Ruimei Wang, Tianzhen Wu, Huan Song, Chuanfeng Liu
Modern Economy (ME) , 2015, DOI: 10.4236/me.2015.65050
Abstract: This research estimated Chinese rural residents’ willingness to pay for rural solid wastes recycling project. Dichotomous choice format contingent valuation method was employed to diverse rural residents’ preference. 4795 Households had been interviewed for collecting primary data, and had been estimated respondents’ will by logistic regression model. It indicated that the respondents would refuse to support solid wastes recycling project due to households’ income restriction. The mean annual WTP of rural residents were 23.41 Chinese Yuan per household. In conclusion the rural solid wastes recycling project is acceptable and accessible for implementation in Chinese rural area.
EXPERIMENTAL STUDY ON THE RELATION BETWEEN THE BURST TENDENCY AND WATER CONTENT IN COAL SEAM
煤层冲击倾向性与含水率关系的试验研究

Mao Xianbiao,Chen Zhanqing,Xu Sipeng,Li Tianzhen,
茅献彪 陈占清 徐思朋
,李天珍

岩石力学与工程学报 , 2001,
Abstract: The relations are determined systematically between the burst tendency and water content, the water content and porosity in coal seam by experiment. It is found that the burst tendency of coal seam depends inversely on the water content, and it is most sensitive in original water content. The saturated water content in coal seam depends on porosity in coal. A reliable quantitative basis is provided for preventing rockburst in coal mine by using water injection method.
Modified fiber qualities of the transgenic cotton ex-pressing a silkworm gene

LI FeiFei,WU ShenJie,,FenNi,CHEN TianZi,JU Ming,WANG HaiHai,JIANG YanJie,ZHANG Jie,GUO WangZhen &,ZHANG TianZhen,

科学通报(英文版) , 2009,
Abstract: A silkworm gene for fibroin was introduced into the upland cotton WC line by Agrobacterium-mediated transformation. PCR detection for fibroin, nptII and gus genes, Kanamycin (Km)-resistance analysis and GUS-histochemical assay were conducted on 30 regenerated plants from 9 callus lines, and 17 positive plants were obtained by these 5 screening methods. By Km-resistance analysis and PCR for fibroin, 6 homozygous lines in T3 were obtained. Southern blot and Northern bolt demonstrated that the fibroin gene was inserted into the genome of these 6 lines, stably inherited and expressed. Compared to the control, the surface structure of mature fiber in the 6 lines was significantly distorted and an increased number of convolution was observed by scanning electron microscopy (SEM). Fiber quality traits analysis indicated that fiber elongation of the 6 homozygous lines was all increased and fiber strength of 3 lines was enhanced. These results indicated that fibroin expression influenced cotton fiber structure and quality, suggesting that fibroin has great potential for improving cotton fiber quality by genetic engineering. Supported by National High Technology Research and Development Program of China (Grant No. 2006AA100105), Science & Technology Pillar Program of Jiangsu Province (Grant No. BE2008310) and Programme of Introducing Talents of Discipline to Universities (Grant No. B08025) Contributed equally to this work
Detection and mapping of homologous and homoeologous segments in homoeologous groups of allotetraploid cotton by BAC-FISH
Kai Wang, Wangzhen Guo, Tianzhen Zhang
BMC Genomics , 2007, DOI: 10.1186/1471-2164-8-178
Abstract: Homologous and homoeologous BACs were isolated by way of SSR marker-based selection and then used to develop BAC-FISH probes. Duplicated segments in homoeologous chromosomes were detected by FISH. The FISH and related linkage map results followed known reinforced the relationships of homoeologous chromosomes in allotetraploid cotton, and presented a useful approach for isolation of homoeologous loci or segments and for mapping of monomorphic loci. It is very important to find that the large duplicated segments (homologous BACs) do exist between homoeologous chromosomes, so the shot-gun approach for genome sequencing was unavailable for tetraploid cotton. However, without doubt, it will contain more information and promote the research for duplicated segments as well as the genome evolution in cotton.These findings and the analysis method by BAC-FISH demonstrated the powerful nature and wide use for the genome and genome evolutionary researches in cotton and other polyploidy species.Polyploidy is an evolutionary process whereby two or more genomes are brought together in the same nucleus, usually by hybridization followed by chromosome doubling [1]. Accordingly, most of these genomes contain duplicated chromosomes or chromosomal segments that reflect ancient or recent rounds of polyploidy. Therefore, investigations of the levels of diversity and patterns of duplicated genes and segments in polyploid plants can provide insights into the process of polyploidization and subsequent processes. When genes are duplicated as a consequence of polyploidization, they may continue to evolve at the same rate as they did in their diploid ancestors, or they may be subject to pressures that lead to differential rates of sequence evolution [2]. Ultimately, these duplicated sequences and their functions are maintained intact or undergo long-term evolutionary change via sequence elimination [3,4], sequence rearrangement [5], gene silencing [6], or acquisition of new function [7]. Most
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