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Search Results: 1 - 10 of 1823 matches for " Leticia Esparza "
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Improving Total-Cholesterol/HDL-Cholesterol Ratio Results in an Endothelial Dysfunction Recovery in Peripheral Artery Disease Patients
Silvia Bleda,Joaquín de Haro,César Varela,Leticia Esparza,Javier Rodriguez,Francisco Acin
Cholesterol , 2012, DOI: 10.1155/2012/895326
Abstract: Aims. To evaluate the effects of variations of total-cholesterol/HDL-cholesterol ratio and the effects of the atorvastatin on endothelial function in peripheral artery disease (PAD). Material and Methods. A prospective, randomised controlled study was carried out in 150 PAD patients. Patients randomized to the control group () were treated with antiplatelet drugs, angiotensin-converting-enzyme inhibitors and cardiovascular-risk-factor control. Experimental group () also received treatment with atorvastatin for a month. It was determined baseline nitrite plasma levels and total-cholesterol/HDL-cholesterol ratio and after one month of treatment in both groups. It was also analysed the correlation between the gradient of nitrite levels and the differential of total-cholesterol/HDL ratio in treatment group. Results. After a month, a reduction in nitrite levels was detected in treatment group ( μM versus 5.7 ± 1.8 μM, ). It was shown a higher decrease in nitrite plasma levels in the atorvastatin group finding lower levels assessments (5.7 ± 1.8 μM versus 13.1 ± 9.1 μM, resp., ). A significant reduction in total-cholesterol/HDL-cholesterol ratio was observed in statin group after treatment (). A strong correlation was found between the gradient of nitrite levels and the differential of total-cholesterol/HDL-cholesterol ratio in atorvastatin group (; ). Conclusions. Improvement of nitrite levels are associated with decreased total cholesterol/HDL ratio values in PAD patients treated with atorvastatin.
Enhanced Vascular Endothelial Growth Factor Gene Expression in Ischaemic Skin of Critical Limb Ischaemia Patients
Silvia Bleda,Joaquín de Haro,Francisco Acin,César Varela,Leticia Esparza
International Journal of Vascular Medicine , 2012, DOI: 10.1155/2012/691528
Abstract: Objectives. To perform a quantitative analysis of the vascular endothelial growth factor (VEGF) gene transcription in the skin of ischemic legs and provide information for VEGF in the pathogenesis in critical limb ischemia (CLI). Methods. Skin biopsies were obtained from 40 patients with CLI. Control samples came from 44 patients with chronic venous disease. VEGF gene expression was analysed using quantitative polymerase chain reaction. Results. Patients with CLI had higher skin VEGF expression than control group (RQ: 1.3 ± 0.1 versus 1, ). Conclusions. We found an association between ischemic skin and an elevated VEGF expression in legs from patients with CLI. These data support that the mechanism for VEGF upregulation in hypoxia conditions is intact and acts appropriately in the ischaemic limbs from patients with CLI. 1. Introduction Angiogenesis has been recently involved in the onset and progression of atherosclerosis. The vascular endothelial growth factor (VEGF) is considered a key regulator of angiogenesis. Several researches have demonstrated hypoxia as the major factor in VEGF upregulation [1]. Its expression is dramatically induced by low oxygen tension in endothelial cells [2]. VEGF increases vascular permeability and induces the migration and proliferation of endothelial cells [3]. It has been proved that VEGF promotes an increase in microvessel density and proliferation of vasa vasorum in atherosclerotic plaques [4]. Critical limb ischemia (CLI) is the most severe clinical manifestation of peripheral artery disease (PAD). Its World incidence has been estimated to be 500 to 800 per million inhabitants per year [5]. Without timely diagnosis and revascularization, patients with CLI are at risk of devastating complications including loss of limb and life. Therapeutic angiogenesis has been assessed as an alternative treatment for CLI before the amputation. Thereby, VEGF is one of the most common growth factors used in therapeutic angiogenesis treatment protocols. Disappointingly, the results arose from the clinical trials in therapeutic angiogenesis are not consistent because some of them have not shown a clear effect of this treatment [6]. A likely explanation for these results may lie in the limited knowledge about the complex pathogenesis of CLI and the exact mechanism of action understanding of the therapeutic angiogenesis factors we dispose so far. On the other hand, recent reports have recently suggested serum VEGF levels to be an indicator of the severity of PAD [7]. CLI is characterized by chronically hypoxic conditions in muscle and
Treatment of thromboangiitis obliterans (Buerger's disease) with bosentan
Joaquin De Haro, Francisco Acin, Silvia Bleda, Cesar Varela, Leticia Esparza
BMC Cardiovascular Disorders , 2012, DOI: 10.1186/1471-2261-12-5
Abstract: A clinical pilot study was designed in which patients with ulcer and/or pain at rest were treated with bosentan p.o. at a dose of 62.5 mg twice daily during the first month, which was thereafter up-titrated to 125 mg twice daily. The study endpoints were clinical improvement rate, major or minor amputation rate, haemodynamic changes, changes in endothelial function and angiographic changes.Seven out of 12 patients were male (58%). Median age was 39 years (range 29-49). The median follow-up was 20 months (range 11-40). All patients were smokers. With bosentan treatment, new ischaemic lesions were observed in only one patient. Overall, clinical improvement was observed in 12 of the 13 extremities (92%). Only two out of 13 extremities underwent amputation (one major and one minor) after bosentan treatment. After being assessed by digital arteriography with subtraction or angio-magnetic resonance imaging, an increase of distal flow was observed in 10 out of the 12 patients. All patients experienced a statistically significant improvement in their BAFMD values (mean: 1.8 at baseline; 6.6 at the end of the treatment; 12.7 three months after the end of the treatment; p < 0.01).Bosentan treatment may result in an improvement of clinical, angiographic and endothelial function outcomes. Bosentan should be investigated further in the management of TAO patients. Larger studies are required to confirm these results.ClinicalTrials.gov: NCT01447550Buerger's disease, also known as thromboangiitis obliterans (TAO), is a thrombotic, occlusive, non-atherosclerotic, segmental vasculitis of small and medium-sized arteries and veins, which may involve both upper and lower extremities. The onset usually occurs in people of around the age of 45, and is more frequent in male smokers. As a consequence of the increase in tobacco use, an increase in the incidence of TAO in women has been observed in the last 20 years [1,2].Intermittent claudication and, in more advanced cases, pain at rest are
Neospora caninum: Detección de ADN en sangre durante la primera gestación de vaquillas infectadas naturalmente
Omar Iván Santana,Carlos Cruz-Vázquez,Leticia Medina-Esparza,Miguel Ramos Parra
Veterinaria México , 2010,
Abstract: El objetivo del trabajo fue detectar la presencia de ADN de N. caninum en animales infectados naturalmente, en dos tiempos de su primera gestación y al parto, así como registrar la presentación de abortos. Se seleccionó, en un establo, con presencia de parasitosis, un lote de 20 hembras de entre 12 y 14 meses de edad, seropositivas en ELISA, las hembras fueron inseminadas artificialmente y se tomaron muestras de sangre en el primero y segundo tercios de gestación y al parto; se aisló ADN y se sometió a PCR anidado en un solo tubo con iniciadores específicos. En el muestreo correspondiente al primer tercio de la gestación, se observaron 7/20 casos positivos (35%), en el segundo 15/20 (75%) y al parto 10/20 casos positivos (50%). De los animales incluidos en el estudio, tres se mantuvieron negativos a la prueba en los tres muestreos (15%), cuatro fueron siempre positivos (20%), ocho fueron positivos en el segundo muestreo pero negativos en el primero (40%) y cinco fueron positivos en el segundo y negativos en el primero y tercero muestreos (25%). No se presentó seroconversión en ningún animal durante el estudio; cuatro de ellos presentaron aborto en el último tercio de gestación. Todas las crías nacidas vivas resultaron seropositivas a N. caninum.
Relationship between white blood cell count and circulating anti-endothelial cell antibodies detected in patients with peripheral arterial disease
Cesar Varela MD,Joaquin de Haro MD,Leticia Esparza MD,Silvia Bleda MD
European Journal of Cardiovascular Medicine , 2012,
Abstract: ABSTRACTBackground and objectives: Circulating anti-endothelial cell antibodies (AECAs) are elevated in peripheral arterial disease (PAD) patients. In this context, these autoantibodies have been associated with the endothelial dysfunction and the pro-inflammatory status that surrounds atherosclerosis. On the other hand, white blood cell (WBC) count and inflammatory markers have been consistently associated with cardiovascular events and PAD. Our aim is to investigate the relationship between circulating AECAs and WBC count in patients with PAD.Methods: An observational translational study was conducted including 34 male patients with intermittent claudication and no previous autoimmune disease. WBC count was measured in all patients. Highly sensitive C-reactive protein levels (hsCRP) were investigated as a surrogate of inflammation. Circulating AECAs titer was detected using indirect immunofluorescence.Results: We found higher hsCRP levels in patients with circulating AECAs (7.60 [4.25-10.20] vs. 4.90 [3.20-7.00] mg/L p=0.02). We also observed a higher monocyte count (0.72 [0.60-1.01] vs. 0.55 [0.45-0.79] X103/μL p=0.03) and eosinophil count (0.28 [0.22-0.36] vs. 0.25 [0.07-0.29] X103/μL p=0.05) in patients with these autoantibodiesConclusions: Circulating AECAs of PAD patients could be associated with the systemic inflammatory status that surrounds the disease and with high monocyte and eosinophil blood cell count.
Circulating Anti-Beta2-Glycoprotein I Antibodies Are Associated with Endothelial Dysfunction, Inflammation, and High Nitrite Plasma Levels in Patients with Intermittent Claudication
Cesar Varela,Joaquin de Haro,Silvia Bleda,Leticia Esparza,Ignacio Lopez de Maturana,Francisco Acin
International Journal of Inflammation , 2013, DOI: 10.1155/2013/268079
Abstract: Our aim is to investigate a possible association of circulating anti-beta2-glycoprotein I antibodies (ABGPI) with the endothelial dysfunction, nitric oxide bioactivity dysregulation, and the inflammatory status that surrounds peripheral arterial disease. We carried out an observational translational study, including 50 male patients with intermittent claudication and a healthy control group of 10 male subjects, age and sex matched with the cases. Flow-mediated arterial dilatation (FMAD) was assessed as a surrogate of endothelial dysfunction, and C-reactive protein (hsCRP) was determined as a marker of inflammation. Nitrite plasma levels were measured by colorimetric analysis. Circulating ABGPI titer was detected with indirect immunofluorescence. Titers <1?:?10 represented the reference range and the lower detection limit of the test. Circulating ABGPI titer ≥1?:?10 was detected in 21 (42%) patients and in none of the control subjects ( ). Patients with ABGPI titer ≥1?:?10 had a lower FMAD ( ). The CRP levels were higher in patients with ABGPI titer ≥1?:?10 ( ). The nitrite plasma levels were higher in patients with ABGPI titer ≥1?:?10 ( ). These data suggest that these circulating ABGPI may collaborate in the development of atherosclerosis; however, further prospective studies are required to establish a causal relationship. 1. Introduction The endothelium is responsible for maintaining the balance between the different factors involved in the vascular wall function. In atherosclerosis, this balance is broken, and the endothelium is no longer able to regulate vascular homeostasis. This situation causes endothelial dysfunction characterised by vasospasm, vasoconstriction, local coagulation alterations, abnormal fibrinolysis, and an increase in arterial wall cell proliferation. Endothelial dysfunction acts as a primary pathogenic event, as it occurs before structural change are evident on angiogram or ultrasound scan, and it is not correlated with the disease’s severity [1]. The loss of endothelial regulation has been attributed to a reduction in nitric oxide bioactivity and to an increased oxygen-free radical formation in the context of the proinflammatory status found in atherosclerosis [2, 3]. On the other hand, there is currently a wide variety of data pointing to a possible autoimmune origin of atherosclerosis [4–11]. This hypothesis is biologically plausible, as chronic vascular inflammation observed in atherosclerosis is based on the dysregulation of the immune system activity. In this context, circulating anti-beta2-glycoprotein I antibodies
Improving Total-Cholesterol/HDL-Cholesterol Ratio Results in an Endothelial Dysfunction Recovery in Peripheral Artery Disease Patients
Silvia Bleda,Joaquín de Haro,César Varela,Leticia Esparza,Javier Rodriguez,Francisco Acin
Cholesterol , 2012, DOI: 10.1155/2012/895326
Abstract: Aims. To evaluate the effects of variations of total-cholesterol/HDL-cholesterol ratio and the effects of the atorvastatin on endothelial function in peripheral artery disease (PAD). Material and Methods. A prospective, randomised controlled study was carried out in 150 PAD patients. Patients randomized to the control group ( ) were treated with antiplatelet drugs, angiotensin-converting-enzyme inhibitors and cardiovascular-risk-factor control. Experimental group ( ) also received treatment with atorvastatin for a month. It was determined baseline nitrite plasma levels and total-cholesterol/HDL-cholesterol ratio and after one month of treatment in both groups. It was also analysed the correlation between the gradient of nitrite levels and the differential of total-cholesterol/HDL ratio in treatment group. Results. After a month, a reduction in nitrite levels was detected in treatment group ( ?μM versus 5.7 ± 1.8?μM, ). It was shown a higher decrease in nitrite plasma levels in the atorvastatin group finding lower levels assessments (5.7 ± 1.8?μM versus 13.1 ± 9.1?μM, resp., ). A significant reduction in total-cholesterol/HDL-cholesterol ratio was observed in statin group after treatment ( ). A strong correlation was found between the gradient of nitrite levels and the differential of total-cholesterol/HDL-cholesterol ratio in atorvastatin group ( ; ). Conclusions. Improvement of nitrite levels are associated with decreased total cholesterol/HDL ratio values in PAD patients treated with atorvastatin. 1. Introduction Peripheral arterial disease (PAD), the clinical manifestation of atherosclerosis in the lower limbs, is caused by a systemic chronic inflammatory state that affects the entire vascular system [1, 2]. Endothelial damage plays a crucial role in the etiopathogenia of this disease [3]. Endothelial dysfunction is considered to be an early event of atherosclerosis, preceding evidence of atherosclerotic plaques on angiography or ultrasound scan. Such endothelial dysfunction has been attributed to an impairment in nitric oxide (NO) bioactivity, and an increase in the formation of reactive oxygen species (ROS) [4]. NO has several antiatherosclerotic effects, such as inhibition of monocyte migration, inhibition of smooth muscle cell proliferation, and inhibition of platelet aggregation [2–4]. Nitrites are the product of the oxidation of the NO derived from the endothelium. Under physiological conditions, 70%–90% of the nitrites in plasma stem from endothelial nitric oxide synthase (eNOS) activity. Patients with PAD have been shown to have increased
Factores de manejo asociados con la seroprevalencia a la infección por Neospora caninum, en ganado lechero de Aguascalientes, México
José de J. Gutiérrez González,Carlos Cruz Vázquez,Leticia Medina Esparza,Arturo Valdivia Flores
Veterinaria México , 2007,
Abstract: Con el objetivo de determinar la posible asociación entre algunos factores de manejo y la seroprevalencia a la infección por N. caninum en ganado lechero de Aguascalientes, México, se tomaron muestras de suero sanguíneo de 381 vacas Holstein distribuidas en 29 establos localizados en ocho de los once municipios de Aguascalientes. Las muestras fueron analizadas por la técnica de ELISA y se estimó la asociación entre la seroprevalencia y los antecedentes de aborto en las vacas, el origen de los reemplazos, la presencia de perros domiciliados en los establos y la disposición de fetos abortados, así como de desechos placentarios. La seroprevalencia a la infección por N. caninum fue de 57.5%, y en los diferentes municipios se observó una variación de 22% a 67%; la seroprevalencia en el grupo de vacas con antecedentes de aborto fue de 69%, mientras que en el grupo sin antecedentes de aborto fue de 48%; la razón de prevalencia fue de 1.4 (P < 0.001), ello sugiere una asociación entre el aborto y la seropositividad. La seroprevalencia de acuerdo con el origen de los reemplazos (propio o adquirido) fue de 57% y 58%, respectivamente; la presencia y ausencia de perros fue de 61% y 54%, respectivamente; por último, para los establos que tenían un programa de manejo de disposición de fetos abortados y desechos placentarios, la seroprevalencia fue de 61%, y 55%, para los que no lo tenían. Con ninguno de estos tres factores fue posible identifi car asociación con la seroprevalencia.
Neospora caninum infection in beef cattle reared under grazing conditions in north-central Mexico
Karina Mondragón-Zavala,Carlos Cruz-Vázquez,Leticia Medina-Esparza,Miguel Ramos-Parra
Revista MVZ Córdoba , 2011,
Abstract: Objetive. To determine the seroprevalence of N. caninum antibodies and prevalence of parasite DNA in blood, and estimate the association between seroprevalence and the potential risk of some factors in beef cattle under grazing conditions in north-central Mexico. Materials and methods. Blood samples from 139 cows and only 10 bulls belonging to 13 farms were collected and evaluated by ELISA test to detect antibodies against N. caninum. Furthermore, to determine the presence of parasite DNA, nested PCR probe was performed on blood samples. Association between potential risk factors and seroprevalence was estimated. Results. Overall seroprevalence was 23% (35/149 samples), while the prevalence of parasite DNA in blood was 28% (42/149 samples). Of the 149 animals examined 28 (19%) were positive to both tests (25 cows and 3 bulls). Concordance between tests was k = 0.63. All herds had seropositive animals with positive parasite DNA detection in blood. The only risk factor identified was the presence of dogs (OR= 2.65). Conclusions. This study showed that bovine neospososis should be considered as an important infectious disease in north-central Mexico herds. Therefore, an epidemiological control should be taken into consideration to avoid the negative effect of this disease on mexican beef industry.
Neospora caninum infection in beef cattle reared under grazing conditions in north-central Mexico
Mondragón-Zavala,Karina; Cruz-Vázquez,Carlos; Medina-Esparza,Leticia; Ramos-Parra,Miguel; García-Vázquez,Zeferino;
Revista MVZ Córdoba , 2011,
Abstract: objetive. to determine the seroprevalence of n. caninum antibodies and prevalence of parasite dna in blood, and estimate the association between seroprevalence and the potential risk of some factors in beef cattle under grazing conditions in north-central mexico. materials and methods. blood samples from 139 cows and only 10 bulls belonging to 13 farms were collected and evaluated by elisa test to detect antibodies against n. caninum. furthermore, to determine the presence of parasite dna, nested pcr probe was performed on blood samples. association between potential risk factors and seroprevalence was estimated. results. overall seroprevalence was 23% (35/149 samples), while the prevalence of parasite dna in blood was 28% (42/149 samples). of the 149 animals examined 28 (19%) were positive to both tests (25 cows and 3 bulls). concordance between tests was k = 0.63. all herds had seropositive animals with positive parasite dna detection in blood. the only risk factor identified was the presence of dogs (or= 2.65). conclusions. this study showed that bovine neospososis should be considered as an important infectious disease in north-central mexico herds. therefore, an epidemiological control should be taken into consideration to avoid the negative effect of this disease on mexican beef industry.
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