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Search Results: 1 - 10 of 27248 matches for " Leonardo Ferreira Dutra "
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Efeito do substrato e da época de coleta dos ramos no enraizamento de estacas de ameixeira (Prunus salicina Lindl.)
Dutra, Leonardo Ferreira;Kersten, Elio;
Ciência Rural , 1996, DOI: 10.1590/S0103-84781996000300003
Abstract: this work was carried out in a greenhouse with intermittent mist out at the plant science department, eliseu maciel agronomy college of federal university of pelotas (brazil), aiming to evaluate the effect of different substratos and time of cutting on rooting of pium cuttings. mild cuttings with 15cm length andapair of ieaves of frontier cultivar were coilected from a commercial orchard eight years old in november 1993, january and march 1994. all cuttings were treated with indolbutiric acid (iba) at 3000ppm concentration introducing about 1.0cm of their basal end in iba powder and placed in tubes on isopor trays containing different substratos: washed mild sand: vermiculite of medium grains; rice husk ash and saw dust, utilized as isolated or as mixtures (1:1 v/v). cuttings were kept on substratos for 60 days. the results demonstrated that the higher rooted cuttings percentage were obtained for cuttings collected in january and in march. the mixture of sand and saw dust gave the higher number of cuttings rooting with 68.22% and 65.99% for january and march.
Influência do ácido indolbutírico e ethefon no enraizamento de estacas de pessegueiro (Prunus persica (L.) Batsch)
Tonietto, Adilson;Dutra, Leonardo Ferreira;Kersten, Elio;
Ciência Rural , 1997, DOI: 10.1590/S0103-84781997000400007
Abstract: this work was conducted at the plant science departament eliseu maciel agronomy college of federal university of pelotas, in greenhouse with intermitent mist, aiming to evaluate the effect of indolebutyric acid and ethephon (2-chloroetyl phosphonic acid) on peach hranch cutting rooting. median cuttings with 15cm length removed from diamante cultivar branch were used. two lateral lesions were made on cutting basis and they were treated with indolebutyric acid in liquid at 0; 1000; 2000; and 3000ppm concentrations and ethefon at 0; 50; 100 and 150ppm concentrations. the immersion time was five seconds and the cuttings were packed in polietylene sacks containing a mixture of sand and rice husk ash substrate (2:1 v/v). results showed that the indolebutyric acid increased the rooted cuttings percentage up to 1752ppm concentration and the number of roots until 1791ppm concentration. the ethephon did not show significant variation for the form and concentrations used.
Produ??o e sobrevivência de miniestacas e minicepas de erva-mate cultivadas em sistema semi-hidrop?nico
Wendling, Ivar;Dutra, Leonardo Ferreira;Grossi, Fernando;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007000200019
Abstract: the objective of this study was to assess the technical viability of semi-hydroponic system for conduction of ilex paraguariensis ministumps, and the answer of these to different nutritional solutions in the successive gatherings, as well as, the survival of the minicuttings produced in this system after rooting. after eleven gatherings, there was 95.6% of ministumps survival, and production of 291minicuttings m-2 of miniclonal garden. the minicuttings survival was 85.6%, with no significant difference between the solutions. the adopted system is technically viable, and lower concentrated solution to conduct the ministumps is recommended.
época de coleta, ácido indolbutírico e triptofano no enraizamento de estacas de pessegueiro
Dutra, Leonardo Ferreira;Kersten, Elio;Fachinello, José Carlos;
Scientia Agricola , 2002, DOI: 10.1590/S0103-90162002000200019
Abstract: the use of seeds to obtain rootstocks originates individuals different from the mother plant. genetical homogenous material can be obtained by cutting. this work evaluates the effects harvest time of peach branches, tryptophan levels and the application of indolebutyric acid (iba) on the rooting of cuttings kept in greenhouse under intermittent mist. cuttings of diamante, capdeboscq and br-2 cultivars were taken from branches half way in the growing season and prepared to be 12cm long, with two basical lateral incisions and one pair of apical leaves (except during winter). the cuttings were treated by immersion of the base for 5 seconds in indolebutyric acid solution at concentrations of 0, 1000, 2000, 3000 and 4000 mg l-1, and kept for 60 days in vermiculite-filled polyethylene bags under intermittent mist. branches were collected and evaluated for endogenous tryptophan level and their correlation on cultivar rooting capacity. there were rooting differences among cultivars. treatment with indolebutyric acid increased rooting percentual, number and dry matter weight of roots. the best results of rooting and number of roots per cutting were obtained in the spring and summer time; greater dry matter weight of the roots occurred during spring time. a negative correlation between endogenous tryptophan levels and rooting cuttings percentual was observed.
The Eucalipt Micropropagation A Micropropaga o de Eucalipto
Leonardo Ferreira Dutra,Ivar Wendling,Gilvano Ebling Brondani
Pesquisa Florestal Brasileira , 2010, DOI: 10.4336/2012.pfb.58.49
Abstract: The Eucalyptus is the genus that has the highest number of researchs in micropropagation. However, there are few effective results obtained with their continuous proliferation. Micropropagation includes disinfection and culture of explants in nutrient medium and aseptic conditions, multiplication in successive subcultures and in vitro or ex vitro rooting. As advantages, the micropropagation allows: high yield per donor plant, freevirus plants, production of saplings at any time of the year, intensive production of healthy saplings in a short period of time and in a limited space, cloning of plants difficult-to-propagate by conventional methods, such as cutting and minicutting, and donor plant preservation without risk of infection. Its disadvantages include the dependence on a tissue culture laboratory, which results in high maintenance costs, as well as the requirement of highly trained workers, high possibility of contamination, variation of conditions within and among clones, the difficulty of finding appropriate culture medium for the species desired and possibility of mutation as a result of the use of growth regulators. Despite having high cost when compared to other cloning methods, the species and hybrids of eucalypt micropropagation is strategic to maintain superior plant material in vitro or to supply the micro-clonal hedge. doi: 10.4336/2009.pfb.58.49 O eucalipto é o gênero florestal mais pesquisado em micropropaga o, entretanto, s o poucos os resultados efetivos obtidos com sua multiplica o contínua. A micropropaga o inclui a desinfesta o e cultivo dos explantes em meio nutritivo e condi es assépticas, a multiplica o dos propágulos em sucessivos subcultivos e o enraizamento in vitro ou ex vitro. Como vantagens, possibilita: alto rendimento por planta matriz; obten o de plantas isentas de viroses; produ o de mudas em qualquer época do ano; produ o intensiva de mudas sadias, em curto período de tempo e espa o reduzido; clonagem de plantas de difícil propaga o vegetativa por métodos convencionais como estaquia e miniestaquia; e preserva o de plantas matrizes sem riscos de infec o. Suas desvantagens incluem a dependência de um laboratório de cultura de tecidos, cuja manuten o implica em altos custos, além da exigência de m o-de-obra altamente especializada; elevada possibilidade de contamina o; varia o de condi es entre e dentro de clones; dificuldade de encontrar o meio adequado para a espécie desejada; e possibilidade de ocorrência de muta es, como resultado do uso de fitorreguladores. Embora tenha alto custo em compara o a
Asexual propagation of Cupressus lusitanica Propaga o assexuada de Cupressus lusitanica
Dagma Kratz,Ivar Wendling,Gilvano Ebling Brondani,Leonardo Ferreira Dutra
Pesquisa Florestal Brasileira , 2010, DOI: 10.4336/2012.pfb.62.161
Abstract: The objective was to evaluate the induction of epicormic shoots in strains and techniques of cutting, grafting and minicutting for Cupressus lusitanica cloning. For cutting, concentrations of IBA and substrates in plants of 5 and 10 years of age were tested. In minicutting evaluated the type of propagules. Grafting seedlings of trees 10 years as grafts for the first sub-culture, and from this, the shoots were collected for the second subculture. The species presented difficult rooting. The grafting showed the best result. Shoots induction of strains showed low viability. doi: 10.4336/2010.pfb.30.62.161
Aplica o de IBA para o enraizamento de miniestacas de Eucalyptus benthamii Maiden & Cambage x Eucalyptus dunnii Maiden = IBA application for rooting of Eucalyptus benthamii Maiden and Cambage x Eucalyptus dunnii Maiden minicuttings
Gilvano Ebling Brondani,Fernando Grossi,Ivar Wendling,Leonardo Ferreira Dutra
Acta Scientiarum : Agronomy , 2010,
Abstract: O Eucalyptus apresenta grande importancia no setor florestal e muitos avan os na área do melhoramento foram conquistados com o advento da biotecnologia. Contudo, alguns genótipos promissores ainda n o possuem protocolos de multiplica o com técnicas de clonagem, como a miniestaquia. Objetivou-se avaliar concentra es de IBA na sobrevivência, enraizamento e vigor vegetativo de miniestacas de E. benthamii x E. dunnii, com a determina o da dose de máxima eficiência técnica. Minicepas dos clones H12, H19 e H20 foram manejadas em minijardim clonal em sistema semi-hidrop nico. Para o enraizamento, mergulhou-se a por o basal da miniestaca em solu es hidroalcoólicas, cujas concentra es foram: 0, 2.000, 4.000, 6.000 e 8.000 mg L-1 de IBA. O experimento foi conduzido no delineamento inteiramente casualizado, sendo constituído por três clones e cinco concentra es de IBA, com cinco repeti es, contendo dez miniestacas por repeti o. O IBA influenciou positivamente os processos rizogênicos das miniestacas com resposta diferenciada entre os clones, a qual variou de 30,32 a 55,45% de enraizamento. Para os clones H12 e H19, os incrementos positivos ocorreram até a maiorconcentra o de IBA. Contudo, a faixa situada entre os tratamentos de 4.000 e 6.000 mg L-1 de IBA promoveu os melhores resultados de enraizamento para o clone H20. Eucalyptus has great importance in the forestry sector and many advances in the area of improvement have been achieved with the advent of biotechnological techniques. However, some promising genotypes still do not have multiplication protocols with cloning techniques, such as minicutting. The study aimed to evaluate IBA concentrations for survival, rooting and vegetative vigor of E. benthamii x E. dunnii minicuttings, and determine the maximum technical efficiency dose. Ministumps H12, H19 and H20 clones were cultivated in a clonal minigarden under a semi-hydroponic system. For rooting, the minicutting basal portion was plunged in hydro-alcoholic solutions, whose concentrations were: 0; 2,000; 4,000; 6,000 and 8,000 mg L-1 of IBA. The experiment was conducted in a completely randomized design, with the factors consisting of three clones and five IBA concentrations, with five replications, containing 10 minicuttings per replication. IBA positively influenced the minicutting rooting processes, with differentiated behavior between the clones, with 30.32 to 55.45% rooting variation. The positives increments occurred until the highest IBA concentration for H12 and H19 clones. However, the range between 4,000 and 6,000 mg L-1 ofIBA treatments p
Utiliza o de diferentes recipientes na propaga o da ameixeira através de estacas
Revista Brasileira de Fruticultura , 2002,
Abstract: O experimento foi desenvolvido em estufa com nebuliza o intermitente, objetivando comparar quatro tipos de recipientes no enraizamento de estacas de ameixeira. Foram utilizados ramos do ano das cultivares Frontier e Reubennel, dos quais foram retiradas estacas da por o mediana, com aproximadamente 12cm de comprimento, um par de folhas secionadas pela metade e duas incis es laterais na base. Posteriormente, as mesmas tiveram sua base tratada por imers o rápida durante 5 segundos em solu o de ácido indolbutírico (AIB), a 2000mg.L-1 e acondicionadas nos seguintes recipientes: tubetes, bandejas de isopor (128 células), sacos plásticos grandes (14 x 20cm) e sacos plásticos pequenos (10 x 17cm), contendo vermiculita como substrato. O delineamento experimental adotado foi o inteiramente casualizado, com 3 repeti es e 12 estacas por repeti o. Após 70 dias, n o foi observada diferen a no percentual de enraizamento entre as cultivares. A bandeja proporcionou o menor enraizamento entre os recipientes, n o havendo diferen a entre os demais. O maior número de raízes foi obtido com a cultivar Frontier. O maior peso da matéria seca das raízes foi obtido com a cultivar Frontier em saco grandes. Maiores comprimentos de raízes foram obtidos nos maiores recipientes. O tubete proporciona maior economia e menor movimenta o de substrato, sem reduzir o enraizamento.
época de coleta, ácido indolbutírico e triptofano no enraizamento de estacas de pessegueiro
Dutra Leonardo Ferreira,Kersten Elio,Fachinello José Carlos
Scientia Agricola , 2002,
Abstract: A utiliza o de sementes para obten o de porta-enxertos gera indivíduos diferentes da planta-m e. Material homogêneo geneticamente pode ser obtido através da propaga o por estaquia. Avaliaram-se os efeitos da época de coleta dos ramos, do teor de triptofano e da aplica o do ácido indolbutírico (AIB) sobre o enraizamento de estacas de pessegueiro mantidas em estufa sob nebuliza o intermitente. Estacas dos cultivares Diamante, Capdeboscq e BR-2 foram retiradas da por o mediana de ramos da esta o de crescimento e preparadas com 12cm de comprimento, duas incis es laterais na base e um par de folhas apicais (exceto no inverno). As estacas foram tratadas imergindo sua base durante 5 segundos em solu o de ácido indolbutírico nas concentra es de 0, 1000, 2000, 3000 e 4000 mg L-1, sendo posteriormente acondicionadas em sacos de polietileno contendo vermiculita como substrato e mantidas durante 60 dias sob nebuliza o intermitente. Em parte dos ramos coletados, foi avaliado o teor endógeno de triptofano e sua correla o com a aptid o dos cultivares para enraizar. Existe diferen a de enraizamento entre os cultivares. O ácido indolbutírico aumenta o percentual de enraizamento, número e peso da matéria seca das raízes. Os melhores resultados de enraizamento e número de raízes por estaca s o obtidos na primavera e ver o, e na primavera o maior peso de matéria seca das raízes. Maiores percentuais de enraizamento, número e peso da matéria seca das raízes foram encontrados nos meses em que havia menores teores de triptofano nos ramos.
In vitro propagation of nidularium fulgens
de Oliveira Paiva,Patrícia Duarte; Coelho Naves,Vanessa; Ferreira Dutra,Leonardo; Paiva,Renato; Pasqual,Moacir;
Interciencia , 2009,
Abstract: nidularium fulgens lem. is a native bromeliad species of the brazilian atlantic forest, used in gardens and vases. like other bromeliad species, plants are extracted from their environment in order to be commercialized. this study tested different concentrations of growth regulators on the in vitro propagation of n. fulgens. pre-established in vitro seedlings were inoculated in ms culture medium with ba (6-benzylaminopurine) added at the concentrations of 0, 0.5, 1.0, 2.0 and 4.0mg·l-1 in combination with naa (naphthaleneacetic acid) at 0, 0.1, 0.5, 1.0mg·l-1 (experiment 1), and with tdz (thidiazuron) at 0, 0.01, 0.02, 0.05 and 0.1mg·l-1 in combination with naa at 0, 0.1, 0.5 and 1.0mg·l-1 (experiment 2). the combination of auxins and cytokinins, especially tdz, is important for micropro-pagation of n. fulgens. higher numbers of shoots were obtained when 0.02mg·l-1 tdz plus 0.5 or 0.1mg·l-1 naa or ms medium was supplemented with 1.0mg·l-1 ba and 0.1mg·l-1 naa. the occurrence of shoots longer than 1.0cm was optimized when ms medium was supplemented with 0.02mg·l-1 tdz and 0.5mg·l-1 naa. roots were not observed when ba or tdz were added to the ms culture medium. plants over 2.0cm were acclimatized, resulting in a 100% survival rate. the use of the produced plants is suggested for in vitro preservation programs of endangered populations.
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