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Search Results: 1 - 10 of 10763 matches for " Lena Carolina; Zapata Lesmes "
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Estudio de cultivos celulares primarios derivados de Lucilia sericata (Diptera: Calliphoridae)
Echeverry Prieto,Lena Carolina; Zapata Lesmes,ángela Cristina; Segura,Alexandra; Bello,Felio;
Revista Ciencias de la Salud , 2009,
Abstract: the main purpose of this study was to obtain primary cell cultures derived from lucilia sericata (diptera: calliphoridae). necrophagous this fly is used for determination of post-mortem interval and larval therapy. since explants embryonated eggs were performed in various culture media (grace schneider, mm/vp12, dmem, grace/l-15 and l-15), supplemented with 20% fetal serum. sterilization of the biological material was carried out by immersing it in formaldehyde and sodium hypochlorite solutions. the cell growth was initiated in the l-15, mm/vp12, and schneider grace/l-15 in an average time of 10 days after completion of planting by the proliferation of groups of colonies scattered on the surface of the boxes crops and also from the endings of larval fragments. the evolution of cell growth to the formation of monolayer semi-confluent was relatively fast, reaching at 3 weeks post-explant. cellular morphology in cultured cells was heterogeneous, especially epithelioid forms, similar to nerve, giant and irregular. comparison of the growth characteristics of these cell cultures with those obtained from other species of flies was more favorable in the evolution of those obtained from l. sericata, on the grounds that the cells are better adapted to the physical-chemical conditions of several culture media. this is the first report of a cell culture-fly family calliphoridae.
How the carotid body works: Different strategies and preparations to solve different problems
ZAPATA,PATRICIO; LARRAíN,CAROLINA;
Biological Research , 2005, DOI: 10.4067/S0716-97602005000400002
Abstract: this is a review of the different experimental approaches developed to solve the problems in our progress towards a comprehensive understanding of how arterial chemoreceptors operate. an analysis is performed of the bases, advantages and limits of the following preparations: studies of ventilatory reflexes originated from carotid bodies (cbs) in the entire animal; recordings of cb chemosensory discharges in situ; cb preparations perfused in situ; cb explants in oculo; cb explants in ovo; cb preparations incubated in vitro; cb preparations superfused in vitro; cb preparations perfused and superfused in vitro; cb tissue slices in vitro; cells acutely dissociated from cbs; cb cells in tissue culture; petrosal ganglia superfused in vitro; petrosal ganglion cells in tissue culture; and co-cultures of cb and sensory ganglion cells. a brief historical account is given of the passage from one preparation to the next one. emphasis is placed on personal experience with the different preparations whenever possible. examples are given of the importance of selecting the appropriate experimental preparation for solving each particular theoretical problem. in fact, brilliant ideas on how the cb works have been unproductive until finding the adequate experimental approach to explore the validity of such ideas.
How the carotid body works: Different strategies and preparations to solve different problems
PATRICIO ZAPATA,CAROLINA LARRAíN
Biological Research , 2005,
Abstract: This is a review of the different experimental approaches developed to solve the problems in our progress towards a comprehensive understanding of how arterial chemoreceptors operate. An analysis is performed of the bases, advantages and limits of the following preparations: studies of ventilatory reflexes originated from carotid bodies (CBs) in the entire animal; recordings of CB chemosensory discharges in situ; CB preparations perfused in situ; CB explants in oculo; CB explants in ovo; CB preparations incubated in vitro; CB preparations superfused in vitro; CB preparations perfused and superfused in vitro; CB tissue slices in vitro; cells acutely dissociated from CBs; CB cells in tissue culture; petrosal ganglia superfused in vitro; petrosal ganglion cells in tissue culture; and co-cultures of CB and sensory ganglion cells. A brief historical account is given of the passage from one preparation to the next one. Emphasis is placed on personal experience with the different preparations whenever possible. Examples are given of the importance of selecting the appropriate experimental preparation for solving each particular theoretical problem. In fact, brilliant ideas on how the CB works have been unproductive until finding the adequate experimental approach to explore the validity of such ideas.
El reto de la valoración de la calidad de vida en mujeres con cáncer de mama
Salas Zapata,Carolina; Grisales Romero,Hugo;
Revista Salud Uninorte , 2010,
Abstract: this paper provides an overview of the state of the art of quality of life among women diagnosed with breast cancer and it describe to various approaches that have been incorporated into the assessment. this aspect is relevant because its related changes in patterns of care that have been implemented especially among people with chronic disease. we conducted a narrative search for scientific articles in indexed databases, journals and texts. it was noted that most of the authors are centered in the concept and multidimensionality of the quality of life and the perception of the individual patient. this assessment is a challenge due to limitations of the instruments that exist for that purpose and the complexity of capturing what patients feel and think after diagnosis. this poses an epistemological dilemma, that is, to assess something subjective which is not quantifiable in nature but their assessment is necessary to analyze the changes in health care. we conclude that in the last decade breast cancer treatment approaches have moved from mere functional support towards a more comprehensive biopsychosocial model. social support networks are an essential element that affects the quality of life of these patients. the health care systems should ensure integral care, focused on early diagnosis and treatment, and continuous improvement of quality of life.
UNC-ANALISTA: HACIA LA CAPTURA DE UN CORPUS DE REQUISITOS A PARTIR DE LA APLICACIóN DEL EXPERIMENTO MAGO DE OZ
Zapata,Carlos Mario; Palacio,Carolina; Olaya,Natalí;
Revista EIA , 2007,
Abstract: requirements elicitation is one of the most important phases in software development process. a good requirements understanding can lead to better software products, achieving satisfaction of stakeholder needs. however, requirements-capture process is sometimes difficult for analysts, because of the face-to-face character of the meetings required for it and because of difficulties of people for expressing clearly their ideas. in this paper we present unc-analista, a proposal for wizard-of-oz experiment focused on the design of a dialogue-controlled system for helping the analyst labor in the requirements elicitation process. with this system will be possible to capture a requirements corpus, for leading a future development of an automatic requirements elicitation system.
Calidad de vida y factores asociados en mujeres con cáncer de mama en Antioquia, Colombia
Salas Zapata,Carolina; Grisales Romero,Hugo;
Revista Panamericana de Salud Pública , 2010, DOI: 10.1590/S1020-49892010000700002
Abstract: objective: to determine the quality of life and some associated factors in women diagnosed with breast cancer enrolled in cancer treatment programs in a colombian province. methods: in a cross-sectional study, the whoqol bref domains related to the quality of life of 220 women with breast cancer who were in treatment were analyzed. first, a descriptive analysis was done of the sociodemographic variables included in the study. this was followed by an analysis of the quality-of-life scores of the patients in terms of their demographic, clinical, and social characteristics. finally, some characteristics were identified that, in combination, explained the patients' quality of life. results: the quality of life was better for women with more schooling, those who were beneficiaries of the contributory health system, those who received support from family members, those from high-income groups, and those whose personal beliefs (religious and spiritual) helped them cope with the disease. belonging to a lower-income group and having a skeptical attitude constituted risk factors for obtaining lower quality-of-life scores. conclusions: the quality of life of breast cancer patients was poorer, both in general and in the physical, psychological, social, and environmental domains, in those who came from more humble levels of society. psychosocial intervention, through patient-centered methods, is proposed as a strategy that can improve patients' quality of life, especially that of lower-income women. it is necessary to strengthen strategies that enable patients to cope with the disease, relying on the moral, social, and spiritual support of their environment as the main resource.
Aislamiento y caracterización de microorganismos con actividad lipolítica provenientes de sedimentos del Humedal El Jaboque (Bogotá, Colombia)
Mora Moreno Lilia Carolina,Valencia Zapata Hernando
Acta Biológica Colombiana , 2006,
Abstract: En el humedal el Jaboque (Engativá) Bogotá, Colombia, se aislaron microorganismos lipolíticos provenientes de sedimentos, mediante la utilización de medios de cultivo selectivos (agar tributirina, agar aceite de girasol). Primero se evaluó la concentración del sustrato (aceite de girasol) en la cual se encontraba el mayor número de morfotipos aislados, luego se comparó la eficiencia de los dos medios de cultivo de acuerdo a la cantidad de morfotipos aislados. Se evaluaron tres concentraciones diferentes de sustrato. Se aisló un total de 18 cepas pertenecientes a los géneros Bacillus, Pseudomonas, Acinetobacter, Streptomyces, Penicillium, Alternaria. Los medios evaluados constituyen alternativas sencillas de aislamiento de microorganismos con actividad lipolítica en sedimentos.
UNC-ANALISTA: HACIA LA CAPTURA DE UN CORPUS DE REQUISITOS A PARTIR DE LA APLICACIóN DEL EXPERIMENTO MAGO DE OZ
Carlos Mario Zapata,Carolina Palacio,Natalí Olaya
Revista EIA , 2007,
Abstract: La obtención de requisitos es una de las etapas más importantes en el proceso de desarrollo del software. Una buena comprensión de los requisitos puede conducir a mejores productos de software que satisfagan las necesidades de los interesados. Sin embargo, el proceso de captura de requisitos se torna difícil para el analista debido, en gran parte, al carácter presencial que tienen las reuniones que se realizan con tal fin y a la dificultad que presentan algunas personas para expresar sus ideas de forma clara. En este artículo se presenta UNC-Analista, una propuesta de experimento Mago de Oz enfocado al dise o de un sistema de diálogo controlado que posibilite la labor del analista durante el proceso de obtención de requisitos. Con este sistema será posible capturar un corpus de requisitos, que servirá como base para la construcción futura de un sistema automático para la obtención de requisitos. Requirements elicitation is one of the most important phases in software development process. A good requirements understanding can lead to better software products, achieving satisfaction of stakeholder needs. However, requirements-capture process is sometimes difficult for analysts, because of the face-to-face character of the meetings required for it and because of difficulties of people for expressing clearly their ideas. In this paper we present UNC-Analista, a proposal for Wizard-of-Oz experiment focused on the design of a dialogue-controlled system for helping the analyst labor in the requirements elicitation process. With this system will be possible to capture a requirements corpus, for leading a future development of an automatic requirements elicitation system.
El reto de la valoración de la calidad de vida en mujeres con cáncer de mama
Carolina Salas Zapata,Hugo Grisales Romero
Revista Salud Uninorte , 2010,
Abstract: Este artículo brinda una síntesis del estado del arte relacionado con la valoración de la calidad de vida de la mujer diagnosticada con cáncer de mama y describe los elementos que se han incorporado en dicha valoración. Este tema es de gran relevancia debido a los cambios que se vienen implementando en los modelos de atención, especialmente de las personas con enfermedades crónicas. Se realizó una búsqueda narrativa de artículos científicos en bases de datos indizadas, revistas y textos. Se observó que la mayoría de los autores parten de las definiciones de calidad de vida, cuyo eje conceptual es la multidimensionalidad del constructo y la percepción individual de la paciente. Dicha valoración constituye un reto debido a las limitaciones de los instrumentos que existen para tal efecto y la complejidad para captar lo que sienten y piensan las pacientes después del diagnóstico. Además, existe un dilema en términos epistemológicos, al querer valorar algo subjetivo (que por naturaleza no es cuantificable) pero que es necesaria su valoración para analizar los cambios en la atención en salud. Se concluye que en la última década ha habido un fortalecimiento importante relacionado con la atención biopsicosocial del paciente oncológico, trascendiendo lo netamente funcional. Las redes de apoyo social son un elemento fundamental en la calidad de vida de estos pacientes. Los sistemas de salud deben garantizarles una atención integral, enfocada hacia el diagnóstico y tratamiento oportuno, y el mejoramiento continuo de su calidad de vida.
Chepe Escobar
Jaime Lesmes
Matemáticas : Ense?anza Universitaria , 2007,
Abstract:
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