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Search Results: 1 - 10 of 82942 matches for " Leila Maria Lopes Bezerra "
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Esporotricose na gesta??o: relato de cinco casos numa epidemia zoonótica no Rio de Janeiro, Brasil
Costa, Rosane Orofino;Bernardes-Engemann, Andrea Reis;Azulay-Abulafia, Luna;Benvenuto, Fabiana;Neves, Maria de Lourdes Palermo;Lopes-Bezerra, Leila Maria;
Anais Brasileiros de Dermatologia , 2011, DOI: 10.1590/S0365-05962011000500020
Abstract: five cases of sporotrichosis occurring in pregnant women in a zoonotic epidemic in rio de janeiro, brazil, are described. the main clinical features, as well as the challenging therapeutic choices for this specific group of patients, are discussed.
Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks
Anderson Messias Rodrigues,Marcus de Melo Teixeira,G. Sybren de Hoog,Tania Maria Pacheco Schubach,Sandro Antonio Pereira,Geisa Ferreira Fernandes,Leila Maria Lopes Bezerra,Maria Sueli Felipe,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002281
Abstract: Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), S?o Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
Proteomics-Based Characterization of the Humoral Immune Response in Sporotrichosis: Toward Discovery of Potential Diagnostic and Vaccine Antigens
Anderson Messias Rodrigues?,Geisa Ferreira Fernandes?,Leticia Mendes Araujo?,Paula Portella Della Terra?,Priscila Oliveira dos Santos?,Sandro Antonio Pereira?,Tania Maria Pacheco Schubach?,Eva Burger?,Leila Maria Lopes-Bezerra,Zoilo Pires de Camargo
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004016
Abstract: Background Sporothrix schenckii and associated species are agents of human and animal sporotrichosis that cause large sapronoses and zoonoses worldwide. Epidemiological surveillance has highlighted an overwhelming occurrence of the highly pathogenic fungus Sporothrix brasiliensis during feline outbreaks, leading to massive transmissions to humans. Early diagnosis of feline sporotrichosis by demonstrating the presence of a surrogate marker of infection can have a key role for selecting appropriate disease control measures and minimizing zoonotic transmission to humans. Methodology We explored the presence and diversity of serum antibodies (IgG) specific against Sporothrix antigens in cats with sporotrichosis and evaluated the utility of these antibodies for serodiagnosis. Antigen profiling included protein extracts from the closest known relatives S. brasiliensis and S. schenckii. Enzyme-linked immunosorbent assays and immunoblotting enabled us to characterize the major antigens of feline sporotrichosis from sera from cats with sporotrichosis (n = 49), healthy cats (n = 19), and cats with other diseases (n = 20). Principal Findings Enzyme-linked immunosorbent assay-based quantitation of anti-Sporothrix IgG exhibited high sensitivity and specificity in cats with sporotrichosis (area under the curve, 1.0; 95% confidence interval, 0.94–1; P<0.0001) versus controls. The two sets of Sporothrix antigens were remarkably cross-reactive, supporting the hypothesis that antigenic epitopes may be conserved among closely related agents. One-dimensional immunoblotting indicated that 3-carboxymuconate cyclase (a 60-kDa protein in S. brasiliensis and a 70-kDa protein in S. schenckii) is the immunodominant antigen in feline sporotrichosis. Two-dimensional immunoblotting revealed six IgG-reactive isoforms of gp60 in the S. brasiliensis proteome, similar to the humoral response found in human sporotrichosis. Conclusions A convergent IgG-response in various hosts (mice, cats, and humans) has important implications for our understanding of the coevolution of Sporothrix and its warm-blooded hosts. We propose that 3-carboxymuconate cyclase has potential for the serological diagnosis of sporotrichosis and as target for the development of an effective multi-species vaccine against sporotrichosis in animals and humans.
Sporothrix schenckii and sporotrichosis
Lopes-Bezerra, Leila M.;Schubach, Armando;Costa, Rosane O.;
Anais da Academia Brasileira de Ciências , 2006, DOI: 10.1590/S0001-37652006000200009
Abstract: for a long time sporotrichosis has been regarded to have a low incidence in brazil; however, recent studies demonstrate that not only the number of reported cases but also the incidence of more severe or atypical clinical forms of the disease are increasing. recent data indicate that these more severe forms occur in about 10% of patients with confirmed diagnosis. the less frequent forms, mainly osteoarticular sporotrichosis, might be associated both with patient immunodepression and zoonotic transmission of the disease. the extracutaneous form and the atypical forms are a challenge to a newly developed serological test, introduced as an auxiliary tool for the diagnosis of unusual clinical forms of sporotrichosis.
Sporothrix schenckii and sporotrichosis
Lopes-Bezerra Leila M.,Schubach Armando,Costa Rosane O.
Anais da Academia Brasileira de Ciências , 2006,
Abstract: For a long time sporotrichosis has been regarded to have a low incidence in Brazil; however, recent studies demonstrate that not only the number of reported cases but also the incidence of more severe or atypical clinical forms of the disease are increasing. Recent data indicate that these more severe forms occur in about 10% of patients with confirmed diagnosis. The less frequent forms, mainly osteoarticular sporotrichosis, might be associated both with patient immunodepression and zoonotic transmission of the disease. The extracutaneous form and the atypical forms are a challenge to a newly developed serological test, introduced as an auxiliary tool for the diagnosis of unusual clinical forms of sporotrichosis.
Differences in Cell Morphometry, Cell Wall Topography and Gp70 Expression Correlate with the Virulence of Sporothrix brasiliensis Clinical Isolates
Rafaela A. Castro, Paula H. Kubitschek-Barreira, Pedro A. C. Teixeira, Glenda F. Sanches, Marcus M. Teixeira, Leonardo P. Quintella, Sandro R. Almeida, Rosane O. Costa, Zoilo P. Camargo, Maria S. S. Felipe, Wanderley de Souza, Leila M. Lopes-Bezerra
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075656
Abstract: Sporotrichosis is a chronic infectious disease affecting both humans and animals. For many years, this subcutaneous mycosis had been attributed to a single etiological agent; however, it is now known that this taxon consists of a complex of at least four pathogenic species, including Sporothrix schenckii and Sporothrix brasiliensis. Gp70 was previously shown to be an important antigen and adhesin expressed on the fungal cell surface and may have a key role in immunomodulation and host response. The aim of this work was to study the virulence, morphometry, cell surface topology and gp70 expression of clinical isolates of S. brasiliensis compared with two reference strains of S. schenckii. Several clinical isolates related to severe human cases or associated with the Brazilian zoonotic outbreak of sporotrichosis were genotyped and clustered as S. brasiliensis. Interestingly, in a murine subcutaneous model of sporotrichosis, these isolates showed a higher virulence profile compared with S. schenckii. A single S. brasiliensis isolate from an HIV-positive patient not only showed lower virulence but also presented differences in cell morphometry, cell wall topography and abundant gp70 expression compared with the virulent isolates. In contrast, the highly virulent S. brasiliensis isolates showed reduced levels of cell wall gp70. These observations were confirmed by the topographical location of the gp70 antigen using immunoelectromicroscopy in both species. In addition, the gp70 molecule was sequenced and identified using mass spectrometry, and the sequenced peptides were aligned into predicted proteins using Blastp with the S. schenckii and S. brasiliensis genomes.
The effectiveness of biofeedback in treatment of women with stress urinary incontinence: a systematic review
Barbosa, Leila Maria Alvares;Lós, Deniele Bezerra;Silva, Ivson Bezerra da;Anselmo, Caroline W. S. Ferreira;
Revista Brasileira de Saúde Materno Infantil , 2011, DOI: 10.1590/S1519-38292011000300002
Abstract: objectives: to assess whether pelvic-floor muscle training associated with biofeedback is more effective in the treatment of women with stress urinary incontinence, when compared to exercise alone. methods: a research of articles published in the last 20 years in lilacs, pubmed and scirus databases, using the descriptors "urinary incontinence, stress" and "biofeedback" was conducted. randomized clinical trials comparing pelvic-floor muscle training with and without biofeedback were included. results: the study involved three randomized clinical trials involving 169 patients. a physical therapist was responsible for conducting the treatment using electromyographic or pressure biofeedback, and exercises were performed in the clinic or at home. the assessment methods, treatment times and protocols used were heterogeneous. statistical analysis and rates of cure/improvement were similar between the groups of articles analyzed. two studies had a score 8, and the third had 6 points according to pedro's scale. conclusions: examination of the studies found suggests that adding biofeedback to pelvic-floor muscle training appears not to bring about a significant difference in terms of the success of treatment, when compared to perineal exercises performed in isolation.
ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY
Meirelles Pereira,Leila Maria; Gon?alves Bezerra,Daniele; Mandarim-de-Lacerda,Carlos Alberto;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000100002
Abstract: forty mature male rats (wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (control, l-name, l-name + enalapril, l-name + verapamil). three aortic wall parameters were analyzed, as follows: the qa(smn) (the number of the tunica media smooth muscle nuclear profiles per area), the sv(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (awt). rats of the l-name group showed high blood pressure and cardiac hypertrophy. the awt, the qa(smn) and the sv(lamellae) showed the same tendency among the groups. the increased awt, or the reduced qa(smn) and the sv(lamellae) in l-name rats were not completely prevented by the administration of enalapril or verapamil. hypertension causes the awt increase by hypertrophy of the lamellar units of the media layer (sv(lamellae) decrease) and the following rarefaction of the smooth muscle nuclei (the qa(smn) decrease). the relative number of the smooth muscle cells (smc) nuclei in l-name rats was not confirmed when this number was corrected by the arterial wall area. this does not support the smc intense proliferation in animals with no deficiency with the l-name dose and the duration used in this experimentation.
ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY ENALAPRIL Y VERAPAMIL ATENUANTES DE LA REMODELACION DE LA PARED AóRTICA EN DIFICIENCIA DE óXIDO NíTRICO
Leila Maria Meirelles Pereira,Daniele Gon?alves Bezerra,Carlos Alberto Mandarim-de-Lacerda
International Journal of Morphology , 2003,
Abstract: Forty mature male rats (Wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil). Three aortic wall parameters were analyzed, as follows: the Q A(SMN) (the number of the tunica media smooth muscle nuclear profiles per area), the S V(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (AWT). Rats of the L-NAME group showed high blood pressure and cardiac hypertrophy. The AWT, the Q A(SMN) and the S V(lamellae) showed the same tendency among the groups. The increased AWT, or the reduced Q A(SMN) and the S V(lamellae) in l-NAME rats were not completely prevented by the administration of enalapril or verapamil. Hypertension causes the AWT increase by hypertrophy of the lamellar units of the media layer (S V(lamellae) decrease) and the following rarefaction of the smooth muscle nuclei (the Q A(SMN) decrease). The relative number of the smooth muscle cells (SMC) nuclei in L-NAME rats was not confirmed when this number was corrected by the arterial wall area. This does not support the SMC intense proliferation in animals with NO deficiency with the L-NAME dose and the duration used in this experimentation. Cuatro grupos de 10 ratas cada uno, adultas, machos (Wistar) se mantuvieron vivas por 40 días, divididas en: Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil. Tres parámetros de la pared de la aorta fueron analizados: el Q A(SMN) (número de núcleos de células musculares lisas de la túnica media por área), la S V(lamellae) (densidad de superficie de las lamelas de la aorta), y AWT ( grosor de la pared de la aorta). Estos tres parámetros mostraron la misma tendencia entre los grupos. Las ratas del grupo L-NAME tuvieron elevación de la presión arterial e hipertrofia cardiaca. El aumento de AWT, o la reducción del Q A(SMN) y la S V(lamellae) en las ratas L-NAME no fue completamente prevenido por la administración de enalapril o verapamil. La hipertensión causó aumento del AWT por hipertrofia de las unidades lamelares de la túnica media (S V(lamellae) disminuyó) y la rarefacción de los núcleos de músculo liso (Q A(SMN)) disminuyó. El número relativo de núcleos de células musculares lisas (SMC) en ratas L-NAME no fue confirmado cuando este número fue corregido por el área de la pared arterial. La hipótesis de intensa proliferación de SMC en animales con deficiencia de ON, no está confirmada con la dosis de L-NAME usada y la duración del tratamiento de esta investigación.
Gerenciamento dos resíduos sólidos dos servi?os de saúde: aspectos do manejo interno no município de Marituba, Pará, Brasil
Sales,Carla Cristina de Lima; Spolti,Gracieli Pamela; Lopes,Maria do Socorro Bezerra; Lopes,David Franco;
Ciência & Saúde Coletiva , 2009, DOI: 10.1590/S1413-81232009000600032
Abstract: medical wastes offer a potential risk to public health and the environment before an inadequate management. this study aims to verify aspects of internal handling of medical wastes in the city of marituba, pará state. by means of questionnaires and field visits, a descriptive and observational study was performed in 13 health establishments in the city. the total volume of generated medical wastes was about 13,000kg/week. there were deficiencies in many stages of the internal handling, for example the internal treatment that was performed in only one of the establishments, external storage made in 4 establishments and in precarious ways, among many others. also, there were conformities as packing in adequate bags and containers as well as common waste separation. in general way, the federal norms were not accomplished and management of medical wastes in health establishments needs adequacy in every stage of the handling in order to control and reduce risks, decreasing the quantity of residues.
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