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Search Results: 1 - 10 of 38556 matches for " Lee Shiow-Ling "
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Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology
Chia-Feng Lo,Chi-Yang Yu,I-Ching Kuan,Shiow-Ling Lee
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms131114889
Abstract: Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant ( p < 0.01). The optimized medium containing 0.65% olive oil ( v/ v), 2.42% tryptone ( w/ v) and 0.15% Tween-80 ( v/ v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
Wound repair and anti-inflammatory potential of Lonicera japonica in excision wound-induced rats
Chen Wei-Cheng,Liou Shorong-Shii,Tzeng Thing-Fong,Lee Shiow-Ling
BMC Complementary and Alternative Medicine , 2012, DOI: 10.1186/1472-6882-12-226
Abstract: Background Lonicera japonica Thunb. (Caprifoliaceae), a widely used traditional Chinese medicinal plant, is used to treat some infectious diseases and it may have uses as a healthy food and applications in cosmetics and as an ornamental groundcover. The ethanol extract of the flowering aerial parts of L. japonica (LJEE) was investigated for its healing efficiency in a rat excision wound model. Methods Excision wounds were inflicted upon three groups of eight rats each. Healing was assessed by the rate of wound contraction in skin wound sites in rats treated with simple ointment base, 10% (w/w) LJEE ointment, or the reference standard drug, 0.2% (w/w) nitrofurazone ointment. The effects of LJEE on the contents of hydroxyproline and hexosamine during healing were estimated. The antimicrobial activity of LJEE against microorganisms was also assessed. The in vivo anti-inflammatory activity of LJEE was investigated to understand the mechanism of wound healing. Results LJEE exhibited significant antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, and Candida tropicalis. The ointment formulation prepared with 10% (w/w) LJEE exhibited potent wound healing capacity as evidenced by the wound contraction in the excision wound model. The contents of hydroxyproline and hexosamine also correlated with the observed healing pattern. These findings were supported by the histopathological characteristics of healed wound sections, as greater tissue regeneration, more fibroblasts, and angiogenesis were observed in the 10% (w/w) LJEE ointment-treated group. The results also indicated that LJEE possesses potent anti-inflammatory activity, as it enhanced the production of anti-inflammatory cytokines that suppress proinflammatory cytokine production. Conclusions The results suggest that the antimicrobial and anti-inflammatory activities of LJEE act synergistically to accelerate wound repair.
Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles
Chi-Yang Yu,Liang-Yu Huang,I-Ching Kuan,Shiow-Ling Lee
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141224074
Abstract: Biodiesel, a non-toxic and biodegradable fuel, has recently become a major source of renewable alternative fuels. Utilization of lipase as a biocatalyst to produce biodiesel has advantages over common alkaline catalysts such as mild reaction conditions, easy product separation, and use of waste cooking oil as raw material. In this study, Pseudomonas cepacia lipase immobilized onto magnetic nanoparticles (MNP) was used for biodiesel production from waste cooking oil. The optimal dosage of lipase-bound MNP was 40% ( w/ w of oil) and there was little difference between stepwise addition of methanol at 12 h- and 24 h-intervals. Reaction temperature, substrate molar ratio (methanol/oil), and water content ( w/ w of oil) were optimized using response surface methodology (RSM). The optimal reaction conditions were 44.2 °C, substrate molar ratio of 5.2, and water content of 12.5%. The predicted and experimental molar conversions of fatty acid methyl esters (FAME) were 80% and 79%, respectively.
Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica
I-Ching Kuan,Chia-Chi Lee,Bing-Hong Tsai,Shiow-Ling Lee,Wei-Ting Lee,Chi-Yang Yu
Energies , 2013, DOI: 10.3390/en6042052
Abstract: We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.
Hepatitis C Virus Infection Influences the S-Methadone Metabolite Plasma Concentration
Shiow-Ling Wu, Sheng-Chang Wang, Hsiao-Hui Tsou, Hsiang-Wei Kuo, Ing-Kang Ho, Sheng-Wen Liu, Ya-Ting Hsu, Yao-Sheng Chang, Yu-Li Liu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069310
Abstract: Background and Objectives Heroin-dependent patients typically contract hepatitis C virus (HCV) at a disproportionately high level due to needle exchange. The liver is the primary target organ of HCV infection and also the main organ responsible for drug metabolism. Methadone maintenance treatment (MMT) is a major treatment regimen for opioid dependence. HCV infection may affect methadone metabolism but this has rarely been studied. In our current study, we aimed to test the hypothesis that HCV may influence the methadone dosage and its plasma metabolite concentrations in a MMT cohort from Taiwan. Methods A total of 366 MMT patients were recruited. The levels of plasma hepatitis B virus (HBV), HCV, human immunodeficiency virus (HIV) antibodies (Ab), liver aspartate aminotransferase (AST) and alanine aminotransferase (ALT), as well as methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpy?rrolidine(EDDP) were measured along with the urine morphine concentration and amphetamine screening. Results Of the 352 subjects in our cohort with HCV test records, 95% were found to be positive for plasma anti-HCV antibody. The liver functional parameters of AST (Wilcoxon Rank-Sum test, P = 0.02) and ALT (Wilcoxon Rank-Sum test, P = 0.04), the plasma methadone concentrations (Wilcoxon Rank-Sum test, P = 0.043) and the R-enantiomer of methadone concentrations (Wilcoxon Rank-Sum test, P = 0.032) were significantly higher in the HCV antibody-positive subjects than in the HCV antibody-negative patients, but not the S-EDDP/methadone dose ratio. The HCV levels correlated with the methadone dose ( = 14.65 and 14.13; P = 0.029 and 0.03) and the S-EDDP/methadone dose ratio ( = ?0.41 and ?0.40; P = 0.00084 and 0.002) in both univariate and multivariate regression analyses. Conclusions We conclude that HCV may influence the methadone dose and plasma S-EDDP/methadone dose ratio in MMT patients in this preliminary study.
Dehydrocostuslactone Suppresses Angiogenesis In Vitro and In Vivo through Inhibition of Akt/GSK-3β and mTOR Signaling Pathways
Chih-Ya Wang, An-Chi Tsai, Chieh-Yu Peng, Ya-Ling Chang, Kuo-Hsiung Lee, Che-Ming Teng, Shiow-Lin Pan
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031195
Abstract: The traditional Chinese medicine component dehydrocostuslactone (DHC) isolated from Saussurea costus (Falc.) Lipschitz, has been shown to have anti-cancer activity. Angiogenesis is an essential process in the growth and progression of cancer. In this study, we demonstrated, for the first time, the anti-angiogenic mechanism of action of DHC to be via the induction of cell cycle progression at the G0/G1 phase due to abrogation of the Akt/glycogen synthase kinase-3β (GSK-3β)/cyclin D1 and mTOR signaling pathway. First, we demonstrated that DHC has an anti-angiogenic effect in the matrigel-plug nude mice model and an inhibitory effect on human umbilical vein endothelial cell (HUVEC) proliferation and capillary-like tube formation in vitro. DHC caused G0/G1 cell cycle arrest, which was associated with the down-regulation of cyclin D1 expression, leading to the suppression of retinoblastoma protein phosphorylation and subsequent inhibition of cyclin A and cdk2 expression. With respect to the molecular mechanisms underlying the DHC-induced cyclin D1 down-regulation, this study demonstrated that DHC significantly inhibits Akt expression, resulting in the suppression of GSK-3β phosphorylation and mTOR expression. These effects are capable of regulating cyclin D1 degradation, but they were significantly reversed by constitutively active myristoylated (myr)-Akt. Furthermore, the abrogation of tube formation induced by DHC was also reversed by overexpression of Akt. And the co-treatment with LiCl and DHC significantly reversed the growth inhibition induced by DHC. Taken together, our study has identified Akt/GSK-3β and mTOR as important targets of DHC and has thus highlighted its potential application in angiogenesis-related diseases, such as cancer.
Synthesis and Antibacterial Activities of Novel 4-Hydroxy-7-hydroxy- and 3-Carboxycoumarin Derivatives
Pen-Yuan Lin,Kuang-Sheng Yeh,Chien-Ling Su,Shiow-Yunn Sheu,Tiffany Chen,Keng-Liang Ou,Mei-Hsiang Lin,Lin-Wen Lee
Molecules , 2012, DOI: 10.3390/molecules170910846
Abstract: Coumarin derivatives are used as fluorescent dyes and medicines. They also have some notable physiological effects, including the acute hepatoxicity and carcinogenicity of certain aflatoxins, the anticoagulant action of dicoumarol, and the antibiotic activity of novobicin and coumerymycin A1. Because the number of drug resistant strains is increasing at present, the synthesis of new antibacterial compounds is one of the critical methods for treating infectious diseases. Therefore, a series of coumarin-substituted derivatives, namely 4-hydroxy- and 7-hydroxycoumarins, and 3-carboxycoumarins were synthesized. 4-Hydroxycoumarin derivatives 4a–c underwent rearrangement reactions. Both 4- and 7-hydroxycoumarins were treated with activated aziridines which produced series of ring-opened products 7, 8, 10, and 11. 3-Carboxy-coumarin amide dimer derivatives 14–21 were prepared by reacting aliphatic alkylamines and alkyldiamines with PyBOP and DIEA. In this study, we use a new technique called modified micro-plate antibiotic susceptibility test method (MMAST), which is more convenient, more efficient, and more accurate than previous methods and only a small amount of the sample is required for the test. Some of the compounds were produced by reactions with acid anhydrides and demonstrated the ability to inhibit Gram-positive microorganisms. The dimer derivatives displayed lower antibacterial activities.
FPGA Implementation of Generalized Hebbian Algorithm for Texture Classification
Shiow-Jyu Lin,Wen-Jyi Hwang,Wei-Hao Lee
Sensors , 2012, DOI: 10.3390/s120506244
Abstract: This paper presents a novel hardware architecture for principal component analysis. The architecture is based on the Generalized Hebbian Algorithm (GHA) because of its simplicity and effectiveness. The architecture is separated into three portions: the weight vector updating unit, the principal computation unit and the memory unit. In the weight vector updating unit, the computation of different synaptic weight vectors shares the same circuit for reducing the area costs. To show the effectiveness of the circuit, a texture classification system based on the proposed architecture is physically implemented by Field Programmable Gate Array (FPGA). It is embedded in a System-On-Programmable-Chip (SOPC) platform for performance measurement. Experimental results show that the proposed architecture is an efficient design for attaining both high speed performance andlow area costs.
Computer-Aided Reconstruction of Traumatic Fronto-Orbital Osseous Defects: Aesthetic Considerations.
Lun-Jou Lo,Yu-Ray Chen,Ching-Shiow Tseng,Ming-Yih Lee
Chang Gung Medical Journal , 2004,
Abstract: Background: In addition to functional goals, a satisfactory aesthetic outcome is importantfor reconstruction of fronto-orbital osseous defects. The purpose of this studyis to report on a method for presurgical fabrication of custom implants using3-dimensional (3-D) imaging data and computer-assisted manufacturingtechniques.Methods: Preoperative 3-D computed tomography data were processed and displayedfor evaluation of defects. Implants were created by a computer-aideddesign/computer-aided manufacturing (CAD/CAM) program. A rapid prototypingsystem was applied for production of the physical models. Rehearsalof surgery was performed using the implants and skull models. Negativecastings were created and were used during the operations to prepare the surgicalimplant utilizing methyl methacrylate. Traumatic fronto-orbital defectsin 4 patients were reconstructed using this method. The follow-up periodranged from 29 to 55 months.Results: Results showed that the custom implants perfectly fit the defects during theoperation. Symmetry and normal fronto-orbital contours were achieved.There were no peri- or postoperative complications. All patients were satisfiedwith the results.Conclusions: Computer-aided presurgical simulation and fabrication of implants is a reliableand effective method for the reconstruction of traumatic fronto-orbitaldefects, with reduced anesthesia time and improved aesthetic outcomes.
Efficient Architecture for Spike Sorting in Reconfigurable Hardware
Wen-Jyi Hwang,Wei-Hao Lee,Shiow-Jyu Lin,Sheng-Ying Lai
Sensors , 2013, DOI: 10.3390/s131114860
Abstract: This paper presents a novel hardware architecture for fast spike sorting. The architecture is able to perform both the feature extraction and clustering in hardware. The generalized Hebbian algorithm (GHA) and fuzzy C-means (FCM) algorithm are used for feature extraction and clustering, respectively. The employment of GHA allows efficient computation of principal components for subsequent clustering operations. The FCM is able to achieve near optimal clustering for spike sorting. Its performance is insensitive to the selection of initial cluster centers. The hardware implementations of GHA and FCM feature low area costs and high throughput. In the GHA architecture, the computation of different weight vectors share the same circuit for lowering the area costs. Moreover, in the FCM hardware implementation, the usual iterative operations for updating the membership matrix and cluster centroid are merged into one single updating process to evade the large storage requirement. To show the effectiveness of the circuit, the proposed architecture is physically implemented by field programmable gate array (FPGA). It is embedded in a System-on-Chip (SOC) platform for performance measurement. Experimental results show that the proposed architecture is an efficient spike sorting design for attaining high classification correct rate and high speed computation.
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