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Search Results: 1 - 10 of 573359 matches for " Laura-Elizabeth; Báez-Salda?a "
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Surveillance of nosocomial infections in a Mexican community hospital: how are we doing?
García-García,Lourdes; Jiménez-Corona,María-Eugenia; Ramírez-López,Laura-Elizabeth; Báez-Saldaa,Renata; Ferreyra-Reyes,Leticia; Ferreira-Guerrero,Elizabeth; Cano-Arellano,Bulmaro; Cruz-Hervert,Pablo; Téllez-Vázquez,Norma Araceli; Verduzco-Rodríguez,Leonardo; Jaramillo-Cosme,Yolanda; Luna-Téllez,Eleazar; León-Rosales,Samuel Ponce de;
Salud Pública de México , 2010, DOI: 10.1590/S0036-36342010000600005
Abstract: objective. to compare the nosocomial infection (ni) rate obtained from a retrospective review of clinical charts with that from the routine nosocomial infection surveillance system in a community hospital. material and methods. retrospective review of a randomized sample of clinical charts.results were compared to standard surveillance using crude and adjusted analyses. results. a total of 440 discharges were reviewed, there were 27 episodes of nis among 22 patients. cumulated incidence was 6.13 ni per 100 discharges. diarrhea, pneumonia and peritonitis were the most common infections. predictors of ni by cox regression analysis included pleural catheter (hr 16.38), entry through the emergency ward, hospitalization in the intensive care unit (hr 7.19), and placement of orotracheal tube (hr 5.54). conclusions. frequency of nis in this community hospital was high and underestimated. we identified urgent needs in the areas of training and monitoring.
A Novel Scoring System to Measure Radiographic Abnormalities and Related Spirometric Values in Cured Pulmonary Tuberculosis
Renata Báez-Saldaa, Yesenia López-Arteaga, Alma Bizarrón-Muro, Elizabeth Ferreira-Guerrero, Leticia Ferreyra-Reyes, Guadalupe Delgado-Sánchez, Luis Pablo Cruz-Hervert, Norma Mongua-Rodríguez, Lourdes García-García
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078926
Abstract: Background Despite chemotherapy, patients with cured pulmonary tuberculosis may result in lung functional impairment. Objective To evaluate a novel scoring system based on the degree of radiographic abnormalities and related spirometric values in patients with cured pulmonary tuberculosis. Methods One hundred and twenty seven patients with cured pulmonary tuberculosis were prospectively enrolled in a referral hospital specializing in respiratory diseases. Spirometry was performed and the extent of radiographic abnormalities was evaluated twice by each of two readers to generate a novel quantitative score. Scoring reproducibility was analyzed by the intra-class correlation coefficient (ICC) and the Bland-Altman method. Multiple linear regression models were performed to assess the association of the extent of radiographic abnormalities with spirometric values. Results The intra-observer agreement for scoring of radiographic abnormalities (SRA) showed an ICC of 0.81 (CI:95%, 0.67–0.95) and 0.78 (CI:95%, 0.65–0.92), for reader 1 and 2, respectively. Inter-observer reproducibility for the first measurement was 0.83 (CI:95%, 0.71–0.95), and for the second measurement was 0.74 (CI:95%, 0.58–0.90). The Bland-Altman analysis of the intra-observer agreement showed a mean bias of 0.87% and -0.55% and an inter-observer agreement of -0.35% and -1.78%, indicating a minor average systematic variability. After adjustment for age, gender, height, smoking status, pack-years of smoking, and degree of dyspnea, the scoring degree of radiographic abnormalities was significantly and negatively associated with absolute and percent predicted values of FVC: -0.07 (CI:95%, -0.01 to -0.04); -2.48 (CI:95%, -3.45 to -1.50); and FEV1 -0.07 (CI:95%, -0.10 to -0.05); -2.92 (CI:95%, -3.87 to -1.97) respectively, in the patients studied. Conclusion The extent of radiographic abnormalities, as evaluated through our novel scoring system, was inversely associated with spirometric values, and exhibited good reliability and reproducibility. As intra-observer and inter-observer agreement of the SRA varied from good to excellent, the use of SRA in this setting appears acceptable.
Differential effect of biotin on carboxylase activity and mice skeletal muscle metabolism  [PDF]
Marco Antonio Parra Montes de Oca, Gabriel Gutiérrez-Ospina, Pablo Hofmann Salcedo, Alma Lilia Fuentes-Farías, Esperanza Meléndez-Herrera, Margarita Gómez-Chavarín, Armida Báez-Saldaa
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.47A2006
Abstract: In mammalian skeletal muscle there are four carboxylases involved in several biochemical processes like gluconeogenesis, tricarboxylic acid cycle anaplerosis, metabolism of fatty acids and metabolism of various amino acids. It has been shown that biotin deficiency reduces body weight at the expense of muscular mass. When necessary, the liver uses skeletal muscle protein to provide glucose and amino acids to organs in need of such compounds. In this paper we analyzed carboxylase specific activities in hind limb skeletal muscle of 3 weeks old BALB/c male mice, at 0, 1, 4, 7, and 14 days of a specific diet with different biotin concentrations. Biotin was used at 0.0, 1.8 or 98.2 mg per kg of food; and was referred to as biotin deficient, sufficient and supplemented, respectively. Water and food supply and consumption by the three groups of mice were the same. Therefore, the observed effects were directly related to biotin ingestion. The body weight of biotin supplemented mice was the same as the body weight of mice in the biotin sufficient group, while biotin deficiency caused body weight reduction after 7 days of biotin depletion. We found that the total protein concentration in the vastus lateralis muscle is associated with the biotin content in the diet. After 7 days, the muscle total protein content was lower in mice of the biotin deficient group while it was higher in the mice from the biotin supplemented group (P < 0.001). Of the four analyzed enzymes, only pyruvate carboxylase specific activity was reduced in both cases: by consuming the supplemented diet and by the lack of this vitamin. Our data show that PC and muscle metabolism are differentially altered by both, biotin excess and biotin deficiency. The mechanisms of these effects are currently under investigation.
Investigación sobre epidemiología convencional y molecular de tuberculosis en Orizaba, Veracruz, 1995-2008
Jiménez-Corona,Ma Eugenia; García-García,Lourdes; León,Alfredo Ponce de; Bobadilla-del Valle,Miriam; Torres,Martha; Canizales-Quintero,Sergio; Palacios-Merino,Carmen; Molina-Hernández,Susana; Martínez-Gamboa,Rosa Areli; Juárez-Sandino,Luis; Cano-Arellano,Bulmaro; Ferreyra-Reyes,Leticia; Cruz-Hervert,Luis Pablo; Báez-Saldaa,Renata; Ferreira-Guerrero,Elizabeth; Sada,Eduardo; Marquina,Brenda; Sifuentes-Osornio,José;
Salud Pública de México , 2009, DOI: 10.1590/S0036-36342009000900013
Abstract: this study describes the achievements of the mexican consortium against tuberculosis, in the sanitary district of orizaba, veracruz, mexico between 1995 and 2008. in brief, the main results can be classified as follows: 1) conventional and molecular epidemiology (measurement of burden of disease, trends, risk factors and vulnerable groups, consequences of drug resistance, identification of factors that favor nosocomial and community transmission); 2) development of diagnostic techniques to detect drug resistance, description of circulating clones and adaptation of simple techniques to be used in the field; 3) evaluation of usefulness of tuberculin skin test, immunologic responses to bcg, impact of directly observed therapy for tuberculosis (dots), and study of immunological biomarkers and 4) comments on ethical aspects of tuberculosis research. additionally, we describe the impact on public policies, transference of technology, capacity building and future perspectives.
Discrepancias entre los datos ofrecidos por la Secretaría de Salud y la Organización Mundial de la Salud sobre tuberculosis en México, 1981-1998
Báez-Saldaa,Renata; Pérez-Padilla,José Rogelio; Salazar-Lezama,Miguel Angel;
Salud Pública de México , 2003, DOI: 10.1590/S0036-36342003000200002
Abstract: objective: to describe the tuberculosis morbidity and mortality trends in mexico, by comparing the data reported by the ministry of health (mh) and the world health organization (who) between 1981 and 1998. material and methods: the number of cases notified in the past few years, their rates, and the trends of the disease in mexico were analyzed. the incidence of smear-positive pulmonary tuberculosis was estimated for 1997 and 1998 with the annual tuberculosis infection risk (atir), to estimate the percentage of bacilliferous cases in 1997-1998. results: who reported more tuberculosis cases for mexico than the mh. however, this difference has decreased throughout the years. the notification of smear-positive cases remained stable during 1993-1998. the estimated percentages of detection were 66% for 1997 and 26% for 1998 (based on atir of 0.5%). tuberculosis mortality decreased gradually (6.7% per year) between 1990 and 1998, whereas the number of new cases increased, suggesting the persistence of disease transmission in the population. conclusions: inconsistencies between case notifications from national data and who were considerable, but decreased progressively during the study period. according to atir estimations, a considerable number of infectious tuberculosis cases are not detected.
Discrepancias entre los datos ofrecidos por la Secretaría de Salud y la Organización Mundial de la Salud sobre tuberculosis en México, 1981-1998
Báez-Saldaa Renata,Pérez-Padilla José Rogelio,Salazar-Lezama Miguel Angel
Salud Pública de México , 2003,
Abstract: OBJETIVO: Describir las tendencias de la morbilidad y mortalidad de la tuberculosis en México, entre 1981 y 1998, comparando datos de la Secretaría de Salud y de la Organización Mundial de la Salud. MATERIAL Y MéTODOS: Se analizó el número de casos y tasas notificados y la tendencia de la enfermedad en los últimos a os. Se calculó la incidencia de casos nuevos de tuberculosis bacilíferos mediante el riesgo anual de infección tuberculosa, con lo que se estimó el porcentaje de detección de casos bacilíferos en 1997-1998. RESULTADOS: El número de casos de tuberculosis emitido por la Organización Mundial de la Salud supera al notificado por la Secretaría de Salud, discrepancia que se ha reducido. Los casos bacilíferos se han mantenido entre 1993-1998 y se estimó una detección de 66 y de 26% en 1997 y 1998, respectivamente (para un Riesgo Anual de Infección Tuberculosa de 0.5%). La mortalidad se redujo 6.7% cada a o entre 1990 y 1998 mientras que se observó un aumento de casos nuevos, lo que implica la persistencia de la transmisión de la infección entre la población. CONCLUSIONES: Hay discrepancia entre el número de casos de tuberculosis ofrecido por la Secretaría de Salud y la Organización Mundial de la Salud. De acuerdo con las estimaciones por el Riesgo Anual de Infección Tuberculosa se deja de detectar un número considerable de casos bacilíferos.
Marine turtle hatchlings use multiple sensory cues to orient their crawling towards the sea: biological and conservation policy implications  [PDF]
Alma Lilia Fuentes-Farias, Gabriel Gutiérrez-Ospina, Esperanza Meléndez Herrera, Verónica Camarena-Ramírez, Gerardo Ochoa-Tovar, Julieta Mendoza-Torreblanca, Raquel Martínez-Méndez, Jaime Urrutia-Fucugauchi, Armida Báez-Saldaa, María Luisa García Zepeda
Advances in Bioscience and Biotechnology (ABB) , 2011, DOI: 10.4236/abb.2011.22008
Abstract: The ability of sea turtle hatchlings to find the seashore soon after hatching is thought to be exclusively dependent upon visual information. Target-oriented movements in most vertebrates, however, relay on combining information gathered through different sensory systems. Hence, in this work, we investigated whether olfactory and/or magnetic information might complement visual cues during hatchling’s seaward crawling. Acute olfactory deprivation and distorted magnetic sensation in visually competent hatchlings resulted in a scattering of seaward crawling routes among cardinal points, some of them being different from those strongly preferred by control hatchlings. In addition, blindfolded hatchlings also displayed a striking misrouting while crawling on the beach surface in spite of having intact olfactory and magnetic senses. Together these results support the notion that visual information is crucial for seaward crawling, but also that olfactory and magnetic information complement visual cues when turtle hatchlings display this behavior. Hence, the present observations suggest that multisensory cues are used by turtle hatchlings while crawling towards the sea. This work also has important implications on the design of species conservation measures and policies. In the near future, efforts must be made to identify and preserve the local natural sources of odors and magnetic cues, in addition to preventing the perturbing effects of artificial lighting on adult and hatchling turtle crawling behavior.
Increased serotonin concentration and tryptophan hydroxylase activity in reproductive organs of copulator males: a case of adaptive plasticity  [PDF]
Ana Ingrid Pichardo, José L. Tlachi-López, Francisco Jiménez-Trejo, Alma L. Fuentes-Farías, Armida Báez-Saldaa, María L. Molina-Cerón, Gabriel Manjarréz-Gutiérrez, Gabriel Gutiérrez-Ospina, Rosa Angélica Lucio
Advances in Bioscience and Biotechnology (ABB) , 2011, DOI: 10.4236/abb.2011.22012
Abstract: Individual male rats may systematically display or not copulatory behavior when paired with receptive females. Although these phenotypes are associated with differences in brain organization and function, they might also do so at the level of the reproductive organs. We then used high performance liquid chromatography to quantify serotonin concentration and the activity of tryptophan hydroxylase in the reproductive organs of copulator and non-copulator males. Sexual behavior display was compared between groups and parameters of fertility and reproductive fitness were determined for copulator males. Copulator males had higher concentrations of serotonin in the epididymis, testicle and ventral prostate than their non-copulator counterparts, as it was found for epididymal and testicular tryptophan hydroxylase activity. However, preliminary data shows that serotonin elevation occurs in copulator males only until they have accumulated several sexual encounters, so it might be a response to genital gratification or sexual rewarding. Interestingly, only epididymal serotonin concentration correlated with reproductive fitness, offspring number, mating success and seminal plug volume in copulator males. Our results support that copulator and non-copulator male rats feature a phenotype-specific serotoninergic tone in the epididymis, testicle and ventral prostate gland. The observation documenting that epididymal serotonin concentration correlated with parameters that monitor male fertility and reproductive fitness in copulator males predicts that epididymal factors increase their chances of parenting offspring.
Sensory neurons in the spinal cord of nominal female embryos in the marine turtle Lepidochelys olivacea respond to shifts in incubation temperature: implications for temperature dependent sex determination  [PDF]
Francisco Jiménez-Trejo, Leonora Olivos-Cisneros, Julieta Mendoza-Torreblanca, Sofía Díaz-Cintra, Esperanza Meléndez-Herrera, Armida Báez-Saldaa, Patricia Padilla Cortés, Gabriel Gutiérrez-Ospina, Alma Lilia Fuentes-Farías
Advances in Bioscience and Biotechnology (ABB) , 2011, DOI: 10.4236/abb.2011.21001
Abstract: Gonadal determination in marine turtles depends on incubation temperature. The mechanisms that spark off this process remain unclear. Previously, we proposed that sensory nerves reaching the gonadal primordium in nominal female embryos of Lepidochelys (L) olivacea may sense and signal incubation temperature. These nerves could later trigger ovarian determination by releasing neurotransmitters in a code constructed based on the thermal information (Gutierrez-Ospina et al., Acetylcholinesterase-positive innervation is present at the undifferentiated stages of the sea turtle Lepidochelys olivacea embryo gonads: implications for temperature-dependent sex determination, J. Comp. Neurol. 410 (1999) 90-98). The hypothesis briefly described, however, has been recently refuted under weak theoretical grounds and experimental misinterpretations (see introduction). Here, we present preliminary results that show that nominal female embryos have sensory neurons located in the dorsal horn laminae I and II of the lumbar spinal cord that display increased c-Fos-like immuno-staining after being incubated either at 15°C or 50°C. Because these spinal neurons are the primary central target of dorsal root ganglion neurons that innervate the urogential crest, these observations keep open the possibility that gonadal sensory nerves indeed signal thermal information that could later be used to trigger or instruct ovarian specification in marine turtles.
Bioavailability of Soy Protein and Corn Zein Films  [PDF]
Laura B. Matthews, M. Elizabeth Kunkel, James C. Acton, Amod A. Ogale, Paul L. Dawson
Food and Nutrition Sciences (FNS) , 2011, DOI: 10.4236/fns.2011.210148
Abstract: The in vivo bioavailability of soy protein isolates and corn zein film material before and after film formation by heat/ pressure was determined. The In vitro digestibility of corn zein before and after film formation was also measured. Films were produced from anhydrous protein with 30% glycerol added using a heat-press method with no use of casting solvents or cross-linking agents. Corn zein raw and film material were ground under liquid nitrogen then digested in two enzyme-acid cocktails with free amino acid analysis before and after digestion. The protein efficiency ratio and net protein ratio were determined for both zein and soy protein using a weanling Sprague-Dawley descended rat feeding study. The In vitro digestibility study indicated that the non-heat pressed corn zein was more digestible than the heat pressed zein. However, the in vivo results indicated that corn and soy protein were equally bioavailable whether they were derived from film mixture prior to or after film formation. Both corn zein and soy protein material had lower protein efficiency ratio than the control diet. Furthermore, soy protein films materials had a higher protein efficiency ratio than corn zein.
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