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Search Results: 1 - 10 of 462372 matches for " Larissa A. Balabanova "
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The Distribution and Substrate Specificity of Extracellular Nuclease Activity in Marine Fungi  [PDF]
Larissa A. Balabanova, Michael V. Pivkin, Valery A. Rasskazov
Open Journal of Marine Science (OJMS) , 2012, DOI: 10.4236/ojms.2012.24022
Abstract: The distribution and specificity of extracellular nucleases produced by marine fungi belonging to eleven genera, namely: Alternaria, Aspergillus, Aureobasidium, Chaetomium, Fusarium, Gliomastix, Humicola, Penicillium, Scopulariopsis, Wardomyces, Periconia, have implied its important function in the organic phosphorus and nitrogen circle in the Ocean. The fungal nucleases of 64 isolates tested were more or less specific for single-stranded DNA with a high preferential specificity towards poly-U substrate with forming of 5’-phosphate mononucleotides. A couple of the nucleases were capable of RNA digesting. The highest level of extracellular nucleolytic ability was observed in Penicillium spp. isolates. The tight correlation found between extracellular nuclease activity and the rate of thymidine uptake by actively growing and sporulating marine fungus Penicillium melinii suggests that this nuclease is required for fulfilling the nucleotide pool of precursors of DNA biosynthesis during transformation of hyphae into the aerial mycelium and conidia in stressful environmental conditions.
Stereochemical course of hydrolytic reaction catalyzed by alpha-galactosidase from cold adaptable marine bacterium of genus Pseudoalteromonas
Irina Y. Bakunina,Larissa A. Balabanova,Vasiliy A. Golotin,Valeriy A. Rasskazov
Frontiers in Chemistry , 2014, DOI: 10.3389/fchem.2014.00089
Abstract: The recombinant α-galactosidase of the marine bacterium (α-PsGal) was synthesized with the use of the plasmid 40Gal, consisting of plasmid pET-40b (+) (Novagen) and the gene corresponding to the open reading frame of the mature α-galactosidase of marine bacterium Pseudoalteromonas sp. KMM 701, transformed into the Escherichia coli Rosetta(DE3) cells. In order to understand the mechanism of action, the stereochemistry of hydrolysis of 4-nitrophenyl α-D-galactopyranoside (4-NPGP) by α-PsGal was measured by 1H NMR spectroscopy. The kinetics of formation of α- and β-anomer of galactose showed that α-anomer initially formed and accumulated, and then an appreciable amount of β-anomer appeared as a result of mutarotation. The data clearly show that the enzymatic hydrolysis of 4-NPGP proceeds with the retention of anomeric configuration, probably, due to a double displacement mechanism of reaction.
Characterization of a Novel Far-Eastern Potato Virus Y Isolates
Yury G. Volkov,Nadezhda N. Kakareka,Larissa A. Balabanova,Zinaida N. Kozlovskaya
Plant Pathology Journal , 2009,
Abstract: Potato Virus Y (PVY) isolates differed in pathogenicity and molecular properties were found in potato and wild plants in the Far East of Russia. The results of linking research of nucleotide sequence similarity and polymorphism of P1 gene region and serological and biological assays suggested that the viruses originated from a recombination and/or host adaptation events involving the ordinary type virus (PVY0) that led to development of necrotic type virus (PVYN/NTN) properties in the Far-Eastern PVY isolates.
Comparative Analysis of Glycoside Hydrolases Activities from Phylogenetically Diverse Marine Bacteria of the Genus Arenibacter
Irina Bakunina,Olga Nedashkovskaya,Larissa Balabanova,Tatyana Zvyagintseva,Valery Rasskasov,Valery Mikhailov
Marine Drugs , 2013, DOI: 10.3390/md11061977
Abstract: A total of 16 marine strains belonging to the genus Arenibacter, recovered from diverse microbial communities associated with various marine habitats and collected from different locations, were evaluated in degradation of natural polysaccharides and chromogenic glycosides. Most strains were affiliated with five recognized species, and some presented three new species within the genus Arenibacter. No strains contained enzymes depolymerizing polysaccharides, but synthesized a wide spectrum of glycosidases. Highly active β- N-acetylglucosaminidases and α- N-acetylgalactosaminidases were the main glycosidases for all Arenibacter. The genes, encoding two new members of glycoside hydrolyses (GH) families, 20 and 109, were isolated and characterized from the genomes of Arenibacter latericius. Molecular genetic analysis using glycosidase-specific primers shows the absence of GH27 and GH36 genes. A sequence comparison with functionally-characterized GH20 and GH109 enzymes shows that both sequences are closest to the enzymes of chitinolytic bacteria Vibrio furnissii and Cellulomonas fimi of marine and terrestrial origin, as well as human pathogen Elisabethkingia meningoseptica and simbionts Akkermansia muciniphila, gut and non-gut Bacteroides, respectively. These results revealed that the genus Arenibacter is a highly taxonomic diverse group of microorganisms, which can participate in degradation of natural polymers in marine environments depending on their niche and habitat adaptations. They are new prospective candidates for biotechnological applications due to their production of unique glycosidases.
Cross Skin Reactivity to Tyrosine Kinase Inhibitors in a Patient with Chronic Myelogenous Leukemia  [PDF]
V. Broshtilova, M. Balabanova
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.47130

Tyrosine kinase inhibitors (TKI) targeting the bcr-abl protein, c-kit and the platelet-derived growth factor receptors, are significant part of the pathogenic therapy of chronic myelogenous leukemia. A broad spectrum of cutaneous side effects has been described with the clinical use of imatinib mesylate, ranging from various acute rashes to toxic epidermal necrolysis. Herein, a case of cross skin toxicity to TKI in a patient with chronic myelogenous leukemia is presented. In the course of imatinib mesylate therapy the patient developed a grade 4 diffuse lichenoid drug eruption. Six months after switching to nilotinib, hyperpigmented macules and patches spread over his trunk and extremities. To date, few cases of cross skin reactivity to imatinib and nilotinib have been described, none of which showing different clinical phenotypes. Further understanding of the underlying mechanisms and leading to the development of skin rashes from different class of TKI is important to highlight new drug targets and modify the current therapies to a level of maximal efficacy.

Emergence of Haemophilus influenzae Strains in the Nasopharynx of Children with Tuberculosis
Alina V. Martynova,Larisa A. Balabanova,Alexei Pruschinskyi
Pulmonary Medicine , 2011, DOI: 10.1155/2011/420284
Abstract: Being rigorously studied, epidemiology aspects of Haemophilus influenzae carriage are still remaining unclear. Especially it concerns such a group as children with low immune status and also such group as children with tuberculosis infection. We examined nasopharyngeal tract of children with tuberculosis infection in remission and checked how often these children are carriers of Haemophilus influenzae strains. Also we gained microbiology characteristics of the isolates and defined the clinical significance of H. influenzae carriage in development of opportunistic infections in children with the tuberculosis infection. 1. Introduction Despite improvements in diagnosis, treatment,and preventive measures, tuberculosis remains an important cause of morbidity globally. In the Primorye Region of far east Russia (population 2000.000), the incidence of tuberculosis in 2008 was 105.7 per 100.000 and with a prevalence of 222 per 100.000. For children under 17 years, the incidence of tuberculosis was 36.6 per 100.000. In 2008, there were 108 cases of tuberculosis in??children under 11 years, and 83 cases in children from 11 to 17 years (Federal Morbidity Report, 2009). Mortality increased by 20.7% compared to 2000. In the pediatric population, pulmonary tuberculosis accounted for 90.9% of all tuberculosis in 2008, up from 72.7% in 1999. It was recognized that children treated for tuberculosis were more often ill with other bacterial respiratory tract infections than children without such comorbidity. There are several possible contributing factors to this, for example, impaired immune status, malnutrition, and low??socioeconomical status of their family. However, other important factors could be the regular antimicrobial (antituberculosis) chemotherapy they receive or attendance at the Pediatric Tuberculosis Center for anything between 20 and 180 days, according to the current tuberculosis treatment protocol. As the main focus for such patients is the treatment of tuberculosis, the problem of other bacterial respiratory tract infections could be underestimated. There are some data on the carriage of bacterial respiratory tract pathogens in children with various comorbidities [1–5], but little information on the carriage of H.influenzae in children with tuberculosis or children from closed communities [5, 6]. Aim We aim to study the prevalance of H. influenzae in the??nasopharynx of children with tuberculosis and to assess antimicrobial susceptibility of these bacteria. 2. Materials and Methods We examined 85 children from the Pediatric Tuberculosis Center (Group
Effects of calcium antagonists of the third generation in patients with arterial hypertension and concomitant chronic obstructive pulmonary disease
Rational Pharmacotherapy in Cardiology , 2010,
Abstract: Aim. To study antihypertensive efficacy and safety of amlodipine maleate as well as its influence on respiratory function, C reactive protein plasma level, glucose and lipid metabolism in patients with arterial hypertension (HT) and chronic obstructive pulmonary disease (COPD).Material and methods. 50 patients with HT 1-3 grade and COPD I-II stage in remission were randomized into two groups. 31 patients of the first group were treated with amlodipine maleate (Stamlo-M) 5-10 mg/d alone or in combination. 19 patients of the second group received any other antihypertensive therapy except dihydropyridine calcium antagonists.Results. Amlodipine in combined therapy has high antihypertensive efficacy providing achievement of target blood pressure levels and improvement of 24-hour blood pressure profile. Amlodipine therapy improved respiratory function and reduced systolic blood pressure in pulmonary artery. Amlodipine has no negative influence on metabolic status, did not increase sympathetic activity, and reduced C reactive protein levels. It demonstrated good tolerability and safety.Conclusion. High antihypertensive efficacy, improvement of respiratory function, and safety allows recommending amlodipine maleate (Stamlo-M) for usage in hypertensive patients with COPD.
Efficacy evaluation of functional motor regulation method with mathematical analysis of EEG
Balabanova V.A.,Kiselev D.A.,Laisheva О.А.,Gubanov V.V.
Saratov Journal of Medical Scientific Research , 2011,
Abstract: Effects of functional motor regulation method have been measured using the spectral and coherent EEG. Obtained results have fully confirmed the hypothesis that the clinical effectiveness of the method is associated with the formation of new regulatory links in the CNS with inclusion of such deep structures as the limbic and diencephalic systems
Anemia aplásica adquirida e anemia de Fanconi - Diretrizes Brasileiras em Transplante de Células-Tronco Hematopoéticas
Medeiros, Larissa A.;Pasquini, Ricardo;
Revista Brasileira de Hematologia e Hemoterapia , 2010, DOI: 10.1590/S1516-84842010005000064
Abstract: the guidelines presented in this article have been prepared and approved in the i meeting of brazilian guidelines in hematopoietic stem cell transplantation (hsct) - rio de janeiro, july 19-21, 2009. the event was sponsored by sbtmo (brazilian society of bone marrow transplantation). in this paper, we treat the severe aplastic anemia (saa), considered a hematological emergency, that when identified and medically treated early, shows a great chance of recovery of the hematopoiesis, either through bone marrow transplantation or immunosuppressive therapy. its objective is to guide the management of the transplantation, and indicate methods of conditioning, according to clinical characteristics of each patient, including the number of transfusions, in order to minimize the primary and secondary rejection, ensuring better overall and disease-free survival observed in literature and already validated by the results in our population. in the fanconi anemia, transplantation is the only curative option for the aplastic bone marrow component, although insignificant for the other components of the syndrome, it also requires skill and agility in finding a donor with important results.
Gêneros textuais e língua inglesa em uso: uma análise das cole es aprovadas pelo PNLD/LE no Brasil Text genre and english language in use: an analysis of the textbooks approved by the Brazilian government (PNLD/LE)
Ana Larissa A. M. Oliveira
Trabalhos em Linguística Aplicada , 2012, DOI: 10.1590/s0103-18132012000200003
Abstract: Este artigo tem como objetivo analisar dois livros de inglês aprovados no Programa Nacional do Livro Didático (PNLD/LE/2011) para o Ensino Fundamental II nas escolas públicas brasileiras, comparando-as com outras duas cole es, publicadas em período anterior ao documento, e bastante utilizadas nas escolas brasileiras. A análise aqui apresentada centra-se na abordagem dos gêneros textuais orais e escritos presente nestas obras, uma vez que essa abordagem encontra-se no cerne da proposta de incorporar uma vis o de ensino de LE centrada na língua em uso, como prática social. Os resultados demonstraram uma tendência positiva de mudan a, resultante da inser o das línguas estrangeiras no PNLD. Essa tendência poderá compor um novo domínio discursivo para o livro didático de língua estrangeira brasileiro, incluindo a reafirma o dos conceitos de gênero textual, tipo textual, oralidade e escrita, por muito tempo, bastante negligenciados. This article aims at analyzing the two English textbooks approved by the National Textbook Program (PNLD/LE/2011) in Brazil. They have been compared to two other textbooks, published in the period prior to the implementation of the Program, and very widely used in Brazilian schools. The present analysis focuses on the spoken and written text genres of the textbooks in focus, since this pedagogical approach lies at the heart of the proposal to incorporate language in use as social practice in the teaching of English. The results showed a trend of positive change, after foreign languages textbooks were incorporated in the National Textbook Program. This trend can help reshape the discourse domain of English textbooks in Brazil, which includes the concepts of text genre and text type, in spoken and written language, which had been neglected for some time.
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