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Search Results: 1 - 10 of 54317 matches for " LU Ya-Hai "
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A review of syntrophic fatty acids oxidation in anoxic paddy soil
水稻土中脂肪酸互营氧化的研究进展

LIU Peng-Fei,LU Ya-Hai,
刘鹏飞
,陆雅海

微生物学通报 , 2013,
Abstract: Flooded rice field is one of major biogenic sources of greenhouse gas CH4. Complex organic matter is degraded to CH4 and CO2 by the co-operation of anaerobic microorganisms of several metabolic guilds involving the syntrophic oxidation of short-chain fatty acids like propionate, butyrate and acetate. Due to the fastidious nature of cultivation, the diversity and ecology of microorganisms involved in syntrophic oxidation in natural environments like paddy soils remain largely unexplored. Stable isotope probing (SIP), which links microbial identity and function, is a powerful tool to investigate the syntrophic oxidation of fatty acids in flooded paddy soils. This article reviews the recent research progresses in the thermodynamic principles, the interspecies interactions in the syntrophic oxidation of fatty acids, and paddy soils employing SIP technology. The knowledge acquired suggests that phylogenetically diverse bacterial groups are active in the syntrophic oxidation: except classic syntrophic bacteria, organisms belonging to uncultivated phylogenetic groups are also detected, which can serve as candidate syntrophs. Among archaea, Methanocella is the major methanogen partner in syntrophic oxidation of different fatty acids, indicating the importance of this group in CH4 production of paddy field soil.
Effects of ambroxol on biofilm adhesion and viability of Pseudomonas aeruginosa quorum sensing defective strain
Qi LU,Hai-ying ZHONG,Li-hua LIN,Ya-yin LIN
Medical Journal of Chinese People's Liberation Army , 2013,
Abstract: Objective To investigate the effects of ambroxol on the biofilm viability and pristine adhesion of Pseudomonas aeruginosa wild (PAO1) and quorum sensing defective strain (QS, gene deletion of lasI and rhlI). Methods The biofilm was treated by different concentrations (0, 1.875, 3.75mg/ml) of ambroxol. The number of colony was measured with agar plate, multifunction fluorometer was used to measure the fluorescence intensity of PAO1 and QS strains at the bottom of 96-well plate. The adhesion ratio (%) was calculated to determine the effects of ambroxol on bacterial biofilm adhesion. Results Ambroxol treatment reduced the survival rate of the mutant strains compared to that of wild strain, even though the QS strain had increased the adhesion in the presence of ambroxol compared to that of wild strain (P<0.05). Conclusion Ambroxol has a property of significantly antagonizing quorum-sensing system, suggesting that it might be of importance in treatment against chronic Pseudomonasaeruginosainfections.
Previously Unrecognized Ornithuromorph Bird Diversity in the Early Cretaceous Changma Basin, Gansu Province, Northwestern China
Ya-Ming Wang, Jingmai K. O'Connor, Da-Qing Li, Hai-Lu You
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0077693
Abstract: Here we report on three new species of ornithuromorph birds from the Lower Cretaceous Xiagou Formation in the Changma Basin of Gansu Province, northwestern China: Yumenornis huangi gen. et sp. nov., Changmaornis houi gen. et sp. nov., and Jiuquanornis niui gen. et sp. nov.. The last of these is based on a previously published but unnamed specimen: GSGM-05-CM-021. Although incomplete, the specimens can be clearly distinguished from each other and from Gansus yumenensis Hou and Liu, 1984. Phylogenetic analysis resolves the three new taxa as basal ornithuromorphs. This study reveals previously unrecognized ornithuromorph diversity in the Changma avifauna, which is largely dominated by Gansus but with at least three other ornithuromorphs. Body mass estimates demonstrate that enantiornithines were much smaller than ornithuromorphs in the Changma avifauna. In addition, Changma enantiornithines preserve long and recurved pedal unguals, suggesting an arboreal lifestyle; in contrast, Changma ornithuromorphs tend to show terrestrial or even aquatic adaptions. Similar differences in body mass and ecology are also observed in the Jehol avifauna in northeastern China, suggesting niche partitioning between these two clades developed early in their evolutionary history.
Meta-Analysis on the Relationship between HLA-DRBl Gene Polymorphism and Cervical Cancer in Chinese Population
Lin-zhen Wei, Hai-lin Wang, Xin Liu, Ya-peng Lu, Fei Xu, Jin-qiu Yuan, Ya-qin Ling
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088439
Abstract: Aim To determine the association between HLA-DRB1 haplotypes and risk of cervical cancer in unselected and samples from Chinese ethnicities. Methods A comprehensive search for articles from their inception to April 1st, 2013 was conducted from PubMed, Medline, Elsevier Science, Springer Link, Cochrane Library database, China biology medical literature database (CBM),China National Knowledge Infrastructure (CNKI),VIP,and Chinese literature database(Wang fang). A total of 1596 patients with cervical cancer and 2048 controls from the 12 studies on the relationship between gene polymorphism of HLA-DRB l and cervical cancer were performed and data were analyzed and processed using Review Manager 5.0 and Stata 11.0. Results Among the 13 family alleles, two (DRB1*03 and DRB1*08) were found to be negatively associated with cervical cancer in all studies or in Uighur subgroups, and two (DRB1*10 and DRB1*15) were positively associated with in all studies or in Uighur subgroups. Among the 25 specific alleles, six (DRB1*0301, *0403,*0404, *0803, *1312 and *1502) were associated with an increased risk cervical cancer in all studies. No significant association was established for other HLA-DRB1 family alleles and specific alleles. Ethnicity partially explained the race influence of DRB1*12, DRB1*14, DRB1*0301, DRB1*0403, DRB1*0404, DRB1*0803, DRB1*1312 and DRB1*1502 phenotypes. Conclusion Our results support the hypothesis that the HLA-DRB1 family alleles and specific alleles might influence the susceptibility or resistance to cervical cancer, suggesting that immune regulation may play a key role in this disease, although further investigations are still needed.
The Association of ADORA2A and ADORA2B Polymorphisms with the Risk and Severity of Chronic Heart Failure: A Case-Control Study of a Northern Chinese Population
Ya-Jing Zhai,Ping Liu,Hai-Rong He,Xiao-Wei Zheng,Yan Wang,Qian-Ting Yang,Ya-Lin Dong,Jun Lu
International Journal of Molecular Sciences , 2015, DOI: 10.3390/ijms16022732
Abstract: The causes of chronic heart failure (CHF) and its progression are likely to be due to complex genetic factors. Adenosine receptors A2A and A2B (ADORA2A and ADORA2B, respectively) play an important role in cardio-protection. Therefore, polymorphisms in the genes encoding those receptors may affect the risk and severity of CHF. This study was a case-control comparative investigation of 300 northern Chinese Han CHF patients and 400 ethnicity-matched healthy controls. Four common single-nucleotide polymorphisms (SNPs) of ADORA2A (rs2236625, rs2236624, rs4822489, and rs5751876) and one SNP of ADORA2B (rs7208480) were genotyped and an association between SNPs and clinical outcomes was evaluated. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the association. The rs4822489 was significantly associated with the severity of CHF after adjustment for traditional cardiovascular risk factors ( p = 0.040, OR = 1.912, 95% CI = 1.029–3.550). However, the five SNPs as well as the haplotypes were not found to be associated with CHF susceptibility. The findings of this study suggest that rs4822489 may contribute to the severity of CHF in the northern Chinese. However, further studies performed in larger populations and aimed at better defining the role of this gene are required.
Combined Pharmacophore Modeling, Docking, and 3D-QSAR Studies of PLK1 Inhibitors
Shuai Lu,Hai-Chun Liu,Ya-Dong Chen,Hao-Liang Yuan,Shan-Liang Sun,Yi-Ping Gao,Pei Yang,Liang Zhang,Tao Lu
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12128713
Abstract: Polo-like kinase 1, an important enzyme with diverse biological actions in cell mitosis, is a promising target for developing novel anticancer drugs. A combined molecular docking, structure-based pharmacophore modeling and three-dimensional quantitative structure-activity relationship (3D-QSAR) study was performed on a set of 4,5-dihydro-1 H-pyrazolo[4,3- h]quinazoline derivatives as PLK1 inhibitors. The common substructure, molecular docking and pharmacophore-based alignment were used to develop different 3D-QSAR models. The comparative molecular field analysis (CoMFA) and comparative molecule similarity indices analysis (CoMSIA) models gave statistically significant results. These models showed good q 2 and r 2 pred values and revealed a good response to test set validation. All of the structural insights obtained from the 3D-QSAR contour maps are consistent with the available crystal structure of PLK1. The contour maps obtained from the 3D-QSAR models in combination with the structure based pharmacophore model help to better interpret the structure-activity relationship. These satisfactory results may aid the design of novel PLK1 inhibitors. This is the first report on 3D-QSAR study of PLK1 inhibitors.
Soluble Expression of Peptide Containing MUC1/Y-specific Epitope in Escherichia coli and Preparation of the Antibody
MUC1/Y特异表位肽在大肠杆菌中的可溶性表达及其抗体的制备

ZHANG Li Xin,LI Chun Hai SUN Li Ya WANG Miao LU Hao Jun,
张立新
,李春海,孙丽亚,王淼,路浩军

生物工程学报 , 2003,
Abstract: MUC1 mucin is a high molecular weight, type I transmembrane glycoprotein. High and aberrant expression of MUC1 is observed in various types of tumors, which make it an ideal target for tumor biotherapy as well as a biomarker for tumor diagnosis and prognosis. MUC1/Y is an isoform of MUC1 generated by alternative splicing. Specific expression of MUC1/Y in breast cancer as well as its involvement in tumor cell signal transduction have been reported. In order to purify peptides containing MUC1/Y-specific epitope in E. coli and prepare MUC1/Y-specific antibody, DNA fragment encoding the MUC1/Y-specific peptide was amplified by PCR using MUC1/Y full length cDNA as the template and cloned into fusion expression vector pGEX-2T, resulting pGEX-Y30. DNA sequencing was performed to confirm the correct amplification and orientation of the target sequence. Competent E. coli DH5alpha was transformed with pGEX-Y30 and the expression was induced for 4-5 hours in 0.2 mmol/L IPTG at 30 degrees C and 37 degrees C. Expressed proteins were released from the cells by ultrasonication or B-PER II reagent treatments. The fusion protein GST-Y30 were purified by affinity and anion exchange columns and identified by SDS-PAGE and Western-blotting. Polyclonal antibody was prepared by immunizing rabbits with the GST-Y30 protein for 4 times with intervals of 3 weeks and purified by GST column. Western blotting, ELISA and immunohistochemistry analysis were carried out using the purified antibody to confirm its MUC1/Y-binding capacity and specificity. The expressed fusion protein GST-Y30 is about 31 kD in size and represented about 20% of total cellular proteins. The majority of the GST-Y30 protein existed as soluble form when the induction was carried out at both 30 degrees C and 37 degrees C. After the two-step purification, the purity of GST-Y30 was about 94%. The titer of polyserum generated by GST-Y30 immunization was 1:320,000 by ELISA. The antiserum showed MUC1/Y specificity and can recognize MUC1/Y on MCF7 cell. The MUC1/Y-specific polyclonal antibody can be used for studying the role of MUC1/Y in carcinogenesis.
The Performance of CDMA Systems withSpace-Time Spreading Approach underthe Conditions of Multi-users and Multipaths
采用空时扩谱技术的CDMA系统在多用户多径条件下的性能

Xiao Yang,Wang Ya-chen,Hu Shao-hai,Lu Ling-yun,
肖扬
,王亚琛,胡绍海,鲁凌云

电子与信息学报 , 2004,
Abstract: The paper studies the performance of CDMA systems with the space-time approach under the conditions of multi-users and multipaths. The space-time spreading scheme with a novel algorithm can improve the downlink performance by using two antenna elements at the base station and one antenna at the mobile terminal. Different from the present other results, the multi-user systems have been analyzed, the detailed theory analysis and expression of bit-error-probability have been provided, based on the scheme of single-user systems. Moreover, the simulation results show that this scheme obtains an improvement of the CDMA system performance comparing to the conventional approaches, demonstrating that it is a practical way to decrease the bit error rate and improve the quality of the downlink of CDMA systems. Meantime, the implementation complexity of the systems has increased, there are many new technical requirements for mobiles to be satisfied, and the STS technique needs to be improved.
Hybrid Systems Model and Optimization Control of Aerial Robotics
空中机器人的混合系统建模与优化控制

LI Jian-qiang,PEI Hai-long,LU Ya-hui,
李坚强
,裴海龙,卢亚辉

计算机科学 , 2010,
Abstract: To solve the optimization control problem of aerial robotics(small unmanned helicopter) nonlinear system with six degree of freedom(DOF),this paper proposed a method that models and lower-orders based on hybrid systems,obtains the maximal controlled invariant sets by states constraint,then solves the optimization problem by mixed integer quadratic programming in the sets.Finally,a model is modeled and simulated,the result proves the validity and efficiency.
Rhabdomyolysis induced by simvastatin-diltiazem interaction in unrecognized hypothyroidism

Ran Zhang,Cai-Yi Lu,Wei Gao,Ya Huang,Yu-Ling Gao,Qiong-Xiang Yang,Hai-Hong Ran,

老年心脏病学杂志(英文版) , 2010,
Abstract: Simvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is widely prescribed to patients with hypercholesteremia and its muscular toxicity has been widely reported. The metabolism of simvastatin depends on the enzymic activity of cytochrome P450 3A4 (CYP3A4) and inhibitors of CYP3A4 can result in clinical events by interacting with simvastatin. Diltiazem is a moderate inhibitor of CYP3A4, which is known to increase the serum concentration of simvastatin. Here we report a patient with unrecognized hypothyroidism who had been stable for more than one year on low-dose simvastatin therapy of hypercholesteremia and rhabdomyolysis occurred with the addition of diltiazem. This is one of scanty reports of rhabdomyolysis induced by simvastatindiltiazem drug interaction, especially in hypothyroid patient. This case reminds the clinicians that although diltiazem as a moderate CYP3A4 inhibitor can be used cautiously with small doses of CYP3A4-dependent statins (eg, simvastatin), these two commonly used drugs should be avoided in hypothyroid patient.
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