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Search Results: 1 - 10 of 115897 matches for " LIU Ru-Lin "
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Characters and Oil Field Trial of Oil-Degrading Bacterium Strain NX-2
解烃细菌NX-2的特性及矿场试验

LIANG Feng-Lai CHENG Shou-Qiang SUN Xue-Lian LIU Ru-Lin,
梁凤来
,程守强,孙雪莲,刘如林

微生物学通报 , 2004,
Abstract: 针对华北油田马二断块的油藏条件和流体特性选育出解烃细菌NX-2。在缺氧条件下对菌的代谢和改善原油性质等方面进行了评价。在油藏温度(87℃)条件下用人造均质岩芯进行的物理模拟驱油实验表明,微生物驱较水驱提高石油采收率7.1%。在马2-410油井进行的吞吐处理试验,共增产原油669t,少产水3,000余吨,表明NX-2菌适应地层条件,具有增采和改善原油性质的作用。
Study on the potassium characteristic of rendzina soil in the sub-tropic Karst of Guangxi
广西亚热带岩溶地区石灰性土壤钾素特征研究

TAN Hong-Wei,ZHOU Liu-Qia ng,XIE Ru-Lin,HUANG Mei-Fu,
谭宏伟
,周柳强,谢如林,黄美福

中国生态农业学报 , 2006,
Abstract: The potassium characteristic of rendzina soil in the sub-tropic Karst of Guangxi was studied. The results show that the soil available potassium content and available potassium release amount can reflect the soil supply potassium capacity for a season crop in the agro-ecological system of sub-tropic Karst. When the soil availble potassium content is 30 -- 32 mg/kg, the crop is very difficult to absorb potassium and the growth is limited seriously. The main nutrient-limited factor of soil is potassium. Phosphorus, surphur, boron and zinc are not enough for crop growth. Slow potassium content and slow release potassium content can not show the soil supply potassium capacity, but they can show the soil sustainable supply potassium capacity. Applying potassium fertilizer is good for the balance of potassium in soil and can increase the crop yield.
Performance of Demulsification by Rhodococcus sp. PR-1
红球菌PR-1菌株破乳性能研究

MA Ting,LIANG Feng-lai,XI Yan-wei,LIU Ru-lin,
马挺
,梁凤来,奚艳伟,刘如林

环境科学 , 2006,
Abstract: Rhodococcus sp. PR-1 with high capability of demulsification on a surfactant-stabilized kerosene-water model emulsion was isolated from sewage of Dagang oil field. It could demulsify the model emulsion completely in 8 hours at 55 degrees C and had better demulsifying capability than chemical demulsifier DGF-01. The freezing-thawing and autoclaving had no effect on the process of demulsification, yet ultrasonic disposal and deal with organic solvent could inhibit its activity. A linear relationship, not the first order reaction that was used in some references, was observed between the percentage of demulsification and reaction time. The demulsifying capability of PR-1 was mainly resulted from the hydrophobic surfaces of microbial cells, which were characterized by the mycolic acids with the carbon-chain-length from 27 to 54. These results provide foundation for biological application on demulsifying crude oil emulsions in produced water of oil field.
Isolation of Functional Bacteria Guided by PCR-DGGE Technology from High Temperature Petroleum Reservoirs
利用PCR-DGGE技术指导高温油藏中功能微生物的分离

WANG Jun,MA Ting,LIU Jing,LIU Qing-kun,ZHAO Ling-xi,LIANG Feng-lai,LIU Ru-lin,
王君
,马挺,刘静,刘清坤,赵玲侠,梁凤来,刘如林

环境科学 , 2008,
Abstract: It is a brand-new method to isolate functional bacteria from high temperature petroleum reservoirs according to the sequence information obtained from PCR-DGGE patterns. Three-set primers of 16S rDNA high variable region, V3, V8, V9, were compared. The results showed that more microbial diversity information could be obtained from the PCR product of V9 region. Sequence analysis indicated that the dominant bacteria in the petroleum reservoir had high sequence similarity with bacteria from alpha, beta, gamma-Proteobacterias and Bacilli from the GenBank database. According to the sequences information, multi-cultivation technology including enrichment cultivation, special cultivation and direct cultivation methods were employed, and finally, five strains (three strains by traditional methods) were isolated from oil-water samples. Among them, three thermophilic hydrocarbon-degrading bacteria, which belonged to Bacillus sp., Geobacillus sp. and Petrobacter sp., respectively, could grow well under 55 degrees C in obligate anaerobic condition. The crude oil could be utilized by these strains with the degradation rate of 56.5%, 70.01% and 31.78% respectively along with the viscosity reduction rate of 40%, 54.55% and 29.09%, meanwhile the solidify points of crude oil were reduced by 3.7, 5.2 and 3.1 degrees C. Therefore, the combination of sequence information from PCR-DGGE and altering cultivation conditions is an available novel method to isolate more functional microorganisms which could be utilized for microbial enhanced oil recovery.
Identification and Characterization of a Novel Thermophilic Geobacillus Degrading Phenol
一株嗜热菌的分离鉴定及其苯酚降解特性

TANG Yun,LIU Mu-Zhi,LIANG Feng-Lai,FENG Lu,LIU Ru-Lin,
唐赟
,刘沐之,梁凤来,冯露,刘如林

微生物学通报 , 2006,
Abstract: 从油田地层水中分离到一株嗜热并高效降解苯酚的BF80菌株,其最适生长和降解苯酚的温度为60℃65℃。利用API50CHB/E系统和16SrDNA序列分析对菌株BF80进行了分类鉴定,该菌株的形态和生理生化特性与Geobacillusthermoglucosidasius基本相同,其16SrDNA序列与GeobacillusthermoglucosidasiusBGSCW95A1(=ATCC43742)的相似性为99·22%。在接种量为1%的条件下,该菌在20h内能完全降解3mmol/L的苯酚;在pH值5·59·0范围内能保持对苯酚良好的降解能力,并在12mmol/L苯酚的无机盐培养基中也能生长和降解苯酚,表明该菌能耐受高浓度苯酚并可用于高温含酚废水的生物处理。
TWO PSEUDOMONAS ACT ON HYDROCARBON AND THEIR SYNERGISTIC EFFECT
两株假单胞菌对烃的作用及其协同效应

SHAN Jin -Yu JIA Ying LIU Jian HUANG Ying LI Yu- Ping CHEN Zhi-Yu LIU Ru-Lin,
单金玉
,贾莹,刘健,黄英,刘如林,李玉萍,陈智宇

微生物学通报 , 2002,
Abstract: 研究了两株假单胞菌K80-A、K80-B对大港油田官69原油的作用效果。实验证明,能产生乳化因子和假溶因子的K80-B菌株对原油粘度的降低率为51.6%、凝固点降低2℃;而只产生表面活性剂的K80-A菌株对同一原油的作用效果分别为43.5%和3℃。当两菌株以等比例混合共同作用原油时,以上指标均有明显提高。说明两菌株具有协同效应。矿场试验证明了混合菌种的优势。
Studies on Cultivation and Core Simulated Test of Bacillus TP-1 for Microbial Profile Modification
油藏调剖菌TP-1的培养条件及岩芯模拟驱油研究

LIU Jing,WANG Jun,ZHANG Hong-Wei,MA Ting,LI Guo-Qiang,LIANG Feng-Lai,LIU Ru-Lin,
刘 静
,王 君,张宏伟,马 挺,李国强,梁凤来,刘如林

微生物学通报 , 2008,
Abstract: 从油井产出水中分离得到一株兼性厌氧芽孢杆菌TP-1,该菌株可在55℃的油藏温度生长,并代谢产生粘性多糖和气体.经培养条件优化,发酵液多糖产量可达5.5 g/L,产气量为22 mL/L.低浓度的CaCl2、MgSO4和AlCl3对多糖的生成有促进作用.岩芯模拟试验表明,该菌的注入可使岩芯压力增加,提高石油采收率7.37%.TP-1是一株性能良好的油藏调剖菌.
STUDIES ON TREATMENT OF OIL FIELD WASTEWATER BY OIL-DEGRADING BACTERIA
解烃细菌处理油田污水的研究

LIANG Feng-Lai,GUAN Ya-Jun,SUN Xue-Song,LIU MO-Han,LIU Ru-Lin,
梁凤来
,管亚军,孙雪松,刘默涵,刘如林

微生物学通报 , 2002,
Abstract: A mixed bacteria culture F6 isolated from oil field wastewater can degrade petroleum hydrocarbons efficiently. The bacteria were suitable to treat oil-polluted wastewater of oil field. Simulated result treating oil-polluted wastewater in laboratory showed that after "XingyiLian" wastewater of Liaohe Oil Field was treated by fluidized-bed bioreactor system with the vehicle of activated carbon , the amount of oil and CODcr of the flow out water were decreased from 45mg/L to 4. 1mg/L and 470mg/L to 42mg/L separately , according with first class standards of Chinese Wastewater Discharge Regulation.
Studies on Cultivation and Core Simulated Test of Bacillus TP-1 for Microbial Profile Modification
油藏调剖菌TP-1的培养条件及岩芯模拟驱油研究

LIU Jing,WANG Jun,ZHANG Hong-Wei,MA Ting,LI Guo-Qiang,LIANG Feng-Lai,LIU Ru-Lin,
刘 静
,王 君,张宏伟,马 挺,李国强,梁凤来,刘如林

微生物学报 , 2008,
Abstract: 从油井产出水中分离得到一株兼性厌氧芽孢杆菌TP-1, 该菌株可在55℃的油藏温度生长, 并代谢产生粘性多糖和气体。经培养条件优化, 发酵液多糖产量可达5.5 g/L, 产气量为22 mL/L。低浓度的CaCl2、MgSO4和AlCl3对多糖的生成有促进作用。岩芯模拟试验表明, 该菌的注入可使岩芯压力增加, 提高石油采收率7.37%。TP-1是一株性能良好的油藏调剖菌。
Characterization of a Thermophilic Geobacillus Strain DM-2 Degrading Hydrocarbons
嗜热地芽孢杆菌DM-2烃降解特性研究

LIU Qing-kun,WANG Jun,LI Guo-qiang,MA Ting,LIANG Feng-lai,LIU Ru-lin,
刘清坤
,王君,李国强,马挺,梁凤来,刘如林

环境科学 , 2008,
Abstract: 从油田地层水中分离到1株嗜热解烃菌(地芽孢杆菌DM-2),通过研究该菌株在不同条件下降解原油的性能以及在最适条件下降解烃的特性发现:地芽孢杆菌DM-2可在45~70℃、pH 4.0~10.0、 0.2%~3.0%的盐离子浓度缺氧条件下降解原油烃类,其降解原油烃的最适温度和pH值分别为60℃和7.0.在此条件下,该菌可乳化液体石蜡作为碳源进行生长;气相色谱和红外光谱进一步分析表明,其能降解原油中的C14~C30的直链烷烃、支链烷烃和芳香烃类,并能降解C16~C36的长链单烷烃,其中对C28的降解量最高,达88.95%.DM-2菌株代谢烷烃的产物之一为脂肪酸,经液相色谱和质谱分析代谢十六烷产生的脂肪酸为乙酸,5 d产量达0.312 g/L,说明其采用次末端氧化途径氧化烷烃,之后进入β-氧化途径代谢.DM-2菌株在高温缺氧的极端环境下对烃的乳化、降解和产酸特性使其在高温石油污水的生物处理和开采重质原油方面具有潜在的应用价值.
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