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Search Results: 1 - 10 of 117419 matches for " LIU Peng-Hu "
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Cloning, structural analyses and expression levels of phosphofructokinase gene in different strains of Volvariella volvacea
草菇磷酸果糖激酶(PFK)基因克隆、结构及不同菌株中表达量分析

LIU Peng-Hu,XIE Bao-Gui,DENG You-Jin,JIANG Yu-Ji,
刘朋虎
,谢宝贵,邓优锦,江玉姬

菌物学报 , 2013,
Abstract:
Purification and Characterization of CarbonylEnantioselective Reductase from Morganella morganii J-8
Morganella morganii J-8羰基不对称还原酶的分离纯化及性质研究

ZHANG Peng-Hu,ZHANG Liang,LU Yan,SHI Gui-Yang,
张鹏华
,张梁,卢燕,石贵阳

微生物学报 , 2007,
Abstract: 由本实验室筛选得到的摩尔摩根氏菌J-8菌株可将底物1-苯基-2-甲氨基丙酮专一性地转化为d-伪麻黄碱。以M. morganii J-8为出发菌株,菌体超声破碎后,经硫酸铵沉淀、Phenyl Superose疏水柱层析、DEAD阴离子柱层析和非变性凝胶电泳四步纯化获得电泳纯羰基不对称还原酶。亚基分子质量为42.5 kD,高效液相色谱分析酶的分子质量约为84.1 kD,初步认为该酶为二聚体蛋白。对所得到的部分纯化酶的酶学性质做了初步研究,纯酶进行基质辅助激光解析电离-飞行质谱分析,比对结果显示为与亮氨酸脱氢酶蛋白有很高相似性。
Purification and Characterization of CarbonylEnantioselective Reductase from Morganella morganii J-8
Morganella morganii J-8羰基不对称还原酶的分离纯化及性质研究

ZHANG Peng-Hu,ZHANG Liang,LU Yan,SHI Gui-Yang,
张鹏华
,张梁,卢燕,石贵阳

生物工程学报 , 2007,
Abstract: 由本实验室筛选得到的摩尔摩根氏菌J-8菌株可将底物1-苯基-2-甲氨基丙酮专一性地转化为d-伪麻黄碱。以M. morganii J-8为出发菌株,菌体超声破碎后,经硫酸铵沉淀、Phenyl Superose疏水柱层析、DEAD阴离子柱层析和非变性凝胶电泳四步纯化获得电泳纯羰基不对称还原酶。亚基分子质量为42.5 kD,高效液相色谱分析酶的分子质量约为84.1 kD,初步认为该酶为二聚体蛋白。对所得到的部分纯化酶的酶学性质做了初步研究,纯酶进行基质辅助激光解析电离-飞行质谱分析,比对结果显示为与亮氨酸脱氢酶蛋白有很高相似性。
Analysis on the ecological vulnerability of the western Hainan Island based on its landscape pattern and ecosystem sensitivity
基于景观格局和生态敏感性的海南西部地区生态脆弱性分析

QIU Peng-Hu,XU Song-Jun,XIE Gen-Zong,TANG Ben-An,BI Hu,YU Long-Shi,
邱彭华
,徐颂军,谢跟踪,唐本安,毕华,余龙师

生态学报 , 2007,
Abstract: The ecological vulnerability of the western Hainan Island was assessed using a combined landscape pattern and ecosystem sensitivity approach.Models were developed by considering the following five factors:(1) reciprocal of fractal dimension(FD),(2) isolation(FI),(3) fragmentation(FN),(4) sensitivity of land desertification(SD),and(5) sensitivity of soil erosion(SW).The major findings of this study include:(1) The vulnerability of various landscape types was in the following decreasing order: farmland > forest land > water area.This suggests that the ecosystems of farmland and forest land are unstable and therefore sensitive to external disturbances;(2) Significantly positive relationships were found between VI(vulnerability of landscape types)and SD,VI and FN,FN and SD,FN and EVI(regional eco-environment vulnerability).This suggests that FN and SD have great impacts on VI and EVI in the western Hainan Island;(3) There is a good agreement between the predicted and actual distribution of EVI zones.EVI value tends to decrease with increasing distance from the coastline and increase with increasing altitude.(4) The landscape pattern and regional ecological vulnerability in the western Hainan Island is predominantly controlled by human activities though physical factors such as topography and ocean influences also play roles in the process;and(5) The establishment of relationships between landscape information and the regional ecological responses will offer a better way to analyze and assess regional eco-environment issues and therefore better guide the ecological construction in the region.
HOMFLY polynomial from a generalized Yang-Yang function
Sen Hu,Peng Liu
Mathematics , 2013,
Abstract: Starting from the free field realization of Kac-Moody Lie algebra, we define a generalized Yang-Yang function. Then for the Lie algebra of type $A_{n}$, we derive braiding and fusion matrix by braiding the thimble from the generalized Yang-Yang function. One can construct a knots invariant $H(K)$ from the braiding and fusion matrix. It is an isotropy invariant and obeys a skein relation. From them, we show that the corresponding knots invariant is HOMFLY polynomial.
Kauffman polynomial from a generalized Yang-Yang function
Sen Hu,Peng Liu
Mathematics , 2014,
Abstract: For the fundamental representations of the simple Lie algebras of type $B_{n}$, $C_{n}$ and $D_{n}$, we derive the braiding and fusion matrices from the generalized Yang-Yang function and prove that the corresponding knot invariants are Kauffman polynomial.
The Pore Structure of Phosphoaluminate Cement  [PDF]
Weilun Wang, Peng Liu, Ming Zhang, Jiashan Hu, Feng Xing
Open Journal of Composite Materials (OJCM) , 2012, DOI: 10.4236/ojcm.2012.23012
Abstract: The effects of curing time and water-to-cement ratio (W/C) on the pore structure of phosphoaluminate cement (PAC) paste are here presented. Based on the adsorption and condensation theory, the adsorption isotherm of hardened paste was tested using the Brunauer-Emmett-Teller (BET) nitrogen adsorption method. The phase composition and morphology of hydration products cured at different times were analyzed using X-ray diffraction (XRD), and a hydration heat test instrument (HHT) was employed to determine the heat of hydration. The effects of curing time and W/C on the pore structure of PAC are significant. The adsorption isotherm is fitted to the second category based on the Brun-auer- Deming-Deming-Teller (BDDT) classification system. Adsorption volume was found to increase with W/C and then decrease with age. The hysteresis loop of PAC is fitted to the H3 type based on International Union of Pure and Applied Chemistry (IUPAC) guidelines, and the adsorption volume and area enclosed by the hysteresis loop were found to increase with W/C and then decrease with age. BET surface and saturated adsorbed volume of PAC both increase with W/C and decrease with curing time, which is attributable to the greater hydration that produces and changes the characteristics of the pore structure.
Estimation of Distribution Algorithm with Multivariate T-Copulas for Multi-Objective Optimization  [PDF]
Ying Gao, Lingxi Peng, Fufang Li, Miao Liu, Xiao Hu
Intelligent Control and Automation (ICA) , 2013, DOI: 10.4236/ica.2013.41009
Abstract:

Estimation of distribution algorithms are a class of evolutionary optimization algorithms based on probability distribution model. In this article, a Pareto-based multi-objective estimation of distribution algorithm with multivariate T-copulas is proposed. The algorithm employs Pareto-based approach and multivariate T-copulas to construct probability distribution model. To estimate joint distribution of the selected solutions, the correlation matrix of T-copula is firstly estimated by estimating Kendall’s tau and using the relationship of Kendall’s tau and correlation matrix. After the correlation matrix is estimated, the degree of freedom of T-copula is estimated by using the maximum likelihood method. Afterwards, the Monte Carte simulation is used to generate new individuals. An archive with maximum capacity is used to maintain the non-dominated solutions. The Pareto optimal solutions are selected from the archive on the basis of the diversity of the solutions, and the crowding-distance measure is used for the diversity measurement. The archive gets updated with the inclusion of the non-dominated solutions from the combined population and current archive, and the archive which exceeds the maximum capacity is cut using the diversity consideration. The proposed algorithm is applied to some well-known benchmark. The relative experimental results show that the algorithm has better performance and is effective.

An Economical Method for Preparative Purification of Five Alkaloids from Coptis Chinensis Franch by High-Speed Counter-Current Chromatography Using Singled Prepared Solvent System by GC  [PDF]
Lianhong Yin, Lina Xu, Xiaona Wang, Binan Lu, Yingnan Li, Mingming Hu, Yuetao Liu, Jinyong Peng
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.24050
Abstract: Coptis chinensis Franch, a widely used Traditional Chinese Medicine, shows various kinds of bioactivity. The major active components of the herb are considered to be alkaloids. Thus, preparative separation of these alkaloids is critical important for further pharmacology and mechanism studies. In the paper, five alkaloids from C. chinensis were purified by HSCCC using the solvent system composed of chloro-form-metha- nol-water (2:1:1, v/v/v) single prepared. The content of each solvent in solvent system were determined by gas chromatography (GC), then according the ratios of solvents in each phase to prepare the mobile and stationary phase respectively. And a comparative study was carried out between together preparation and single preparation of the solvent system. The purities and recoveries of all the products were over 98.5% and 92%. However, 134 mL chloroform, 336 mL methanol and 452 mL water were saved when the two phase were singled by GC. Our research showed an economical method for separating alkaloids from C. chinensis by HSCCC using the solvent system single prepared by GC.
Duchesnea Phenolic Fraction Inhibits Tumor Growth through Restoring the Th1/Th2 Balance in U14 Cervical Cancer Bearing Mice  [PDF]
Bo Peng, Qin Hu, Lihua Sun, Xinmin Liu, Jianrong Li, Qi Chang, Liwei Wang, Jintian Tang
Chinese Medicine (CM) , 2012, DOI: 10.4236/cm.2012.31007
Abstract: Duchesnea indica (Andr.) Focke has been traditionally used to treat cancer in Asian countries for centuries. In the present study, transplanted U14 cervical cancer mouse model was used to evaluate the antitumor and immunomodulatory activity of Duchesnea phenolic fraction (DPF). ELISA and RIA assay were employed to measured the serum concentration of Th1/Th2 cytokines (IL-2, IL-4, IFN-γ and TNF-α). Administration with 0.25 g/kg, 0.5 g/kg and 1 g/kg DPF significantly reduced the tumor weight by 34.37%, 43.89% and 56.28%, respectively, as compared to the tumor control group. Furthermore, the serum level of IL-2, IFN-γ and TNF-α increased and IL-4 level decreased in a dose-dependent manner during DPF treatment, indicating that the antitumor activity of DPF may be associated with the decrease of TNF-α level and restoration of the balance of Th1/Th2 cell responses. These data suggested that DPF, a mixture of plant polyphenols, had potent anticancer activity which was in part accomplished by its immunomodulatory ability.
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