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Search Results: 1 - 10 of 127103 matches for " LI Xuede "
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Cloning of fiber-specific cDNAs and their structural variations in 4 fiber mutants
Xuede Wang,Yuxian Zhu,Daofan Ji,Shuli Jiang,Yueyou Li
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187176
Abstract: A mRNA preferentially expressed in cotton fiber was cloned from fiber total RNA of normal upland cotton TM-1 (wild-type) by using RT-PCR and corresponding cDNA (signed asTM-E6) was sequenced.TM-E6 gene had no intron and contained an open reading frame of 771 bp long, and might encode a peptide of 246 amino acids. Other 4 genes,Fl-E6, Li-E6, N-E6 andBl-E6, which were homologous toTM-E6 gene, were also isolated from 4 fiber mutants of Fiberless Xu-zhou 142, Ligon lintless, Naked seed and Brown lint, respectively. Sequence analysis of each of these mutant genes revealed many variations in structure and nucleotide composition of gene when compared with the sequence ofTM-E6 gene. (i) There was a changeable repetitive segment in which GGCTCA (Gly-Ser) was repeated 3–5 times between the 82nd and the 93rd codons in different mutant genes. Since the change of Gly-Ser repetitive segment occurred not only in the mutants but also in the wild-type cotton, the repeat frequency might not be associated with the mutation of fiber characteristics, (ii) Among the 4 mutant genes, the percentage of changed codons was 7.05% inFl-E6, 4.98% inLi-E6, and 4.15% inN-E6 andBl-E6. It seems that the percentage of changed codons inE6 sequence was positively correlated to the degree of fiber morphological variation, (iii) E6 polypeptides of two long-fiberless mutants (Fiberless Xuzhou 142 and Ligon lintless) contained high similar (99.4%) variation in the region of 1–174 amino acids from N-terminus, and those of short-fiberless mutants (Fiberless Xuzhou and naked seed) revealed identical variation in the region of 116th–220th amino acids. It also seems that there was a parallel relation between E6 protein variation and fiber phenotype mutation, (iv)Li-E6 andBl-E6 genes also expressed at low level in seed coat besides at high level in fiber.
mRNA differential display between sterile and fertile anther of rice and analysis of cDNA differential fragments
Xuede Wang,Yingguo Zhu
Science China Life Sciences , 1998, DOI: 10.1007/BF02882730
Abstract: Rice anther and leaf mRNAs from two cytoplasmic male sterile lines (Maxie A and Congguong 41A) were compared with those from their maintainers and F1 hybrids by using mRNA differential display to study gene expression pattern in sterile anther during pollen abortion. Anther cDNA differential bands between sterile and fertile plants were more than those of leaf. It was indicated that the expression of fertility gene(s) in the anther was more activable and sufficient than in the leaf. The gene transcript pattern in one of different types of anther was not only associated with its pollen sterility degree but also with its stage of pollen abortion. The anthers with full or partial sterility or with early abortion produced more cDNA differential bands than those fertile or late abortion anthers. In twelve recovered differential cDNA fragments, two were probably associated with male sterility, i. e. one is (AB4A5), which was specifically expressed in the sterile anther, and the other (AB3B2), which contained some sequence homologous to a mitochondrial gene (coxII) and whose expression was partially suppressed in the sterile anther.
Sorption of Cu( Ⅱ ) and Cd( Ⅱ ) by natural biofilms
自然水体生物膜对铜(Ⅱ)、镉(Ⅱ)的吸附研究

TIAN Chao,ZHANG Yuanyuan,QIU Zhiguo,LI Xuede,
田超
,张园园,邱治国,李学德

环境科学学报 , 2007,
Abstract: With high adsorption capability and adsorption surface area, biofilms are actively researched, especially in the area of adsorption of heavy metals. Heavy metal adsorption is greatly affected by the method of developing the biofilm. A biofilm was developed in Dongpu reservoir on the surface of a cotton thread. The characteristics and thermodynamics of Cu and Cd adsorption on the biofilm were studies. Statistical analysis of the adsorption data showed that the thermodynamic data for each metal fit the Freundlich(F) and Langmuir(L) isotherms. The capacity of adsorption of Cu was greater than that for Cd. The adsorption of both Cu and Cd reached equilibrium in less than one hour and fit a second-order kinetics equation. The adsorption of Cu and Cd increased with pH up to pH 7.
Photochemical degradation of chlorothalonil in aqueous solution
百菌清(chlorothalonil)在水中的光化学降解

LI Xuede,HUA Rimao,YUE Yongde,LI Ying,TANG Feng,TANG Jun,
李学德
,花日茂,岳永德,李瑛,汤锋,唐俊

应用生态学报 , 2006,
Abstract: The study on the effects of light source, solution pH and temperature, and surfactant on the photochemical degradation of chlorothalonil showed that the half-life of chlorothalonil photodegradation under high pressure mercury lamp (HPML), UV lamp and sunlight was 22.4, 82.5 and 123.8 min, respectively. Under HPML and sunlight, chlorothalonil had a higher photolysis rate in alkaline solution than in neutral and acid solution. The photolysis rate increased with increasing solution temperature in the range of 10 degrees C - 40 degrees C, which was doubled when the temperature increased every 10 degrees C. Sodium laurylsulfonate (SDS), sodium dodecylbenzene sulfonate (SDBS), Tween 60 and Span 20 showed significant photosensitizing effects, while cetyltrimethylammonium bromide (CTAB) had significant photoquench effect on the photolysis of chlorothalonil.
Slope weighted scoring method for eutrophication comprehensive evaluation of lake
湖泊富营养化综合评价的坡度加权评分法

DENG Dapeng,LIU Gang,LI Xuede,TANG Feng,HUA Rimao,
邓大鹏
,刘刚,李学德,汤锋,花日茂

环境科学学报 , 2006,
Abstract: There are different widths among 10 intervals of each assessment indicator through analysis on Control Standard for Eutrophication Surface Water, which indicate weight of each indicator should be changed with its monitored value. Based on above thoughts, a novel method, named Slope Weighted Scoring Method, is proposed for eutrophication comprehensive evaluation of lakes on the basis of Interpolation Scoring Method. All boundary data listed in assessment standard are normalized to eliminate influence of dimension. Slopes of 10 intervals of each indicator are calculated with normalized boundary data. First step of using this method is calculating corresponding trophic state score of each indicator with interpolation method according to its monitored value and assessment standard. The slope of each indicator is recorded simultaneously. Second step is allocating weight for each indicator with all recorded slopes. Finally, comprehensive trophic state score is obtained with weighted mean method according to scores and weights of all indicators. The proposed method makes the most of slopes of all indicators' intervals in assessment standard to allocate the weight of each indicator dynamically. The character of weights dynamic allocation answers to the nonlinear mapping essence of eutrophication comprehensive evaluation. Applying this method, Interpolation Method and Comprehensive Trophic Level Index Method to evaluate eutrophication of Chaohu Lake, the results showed that there was significant difference between this method and Interpolation Scoring Method, and the most significant difference between this method and Comprehensive Trophic Level Index Method.
Observation of fiber ultrastructure of Ligon lintless mutant in upland cotton during fiber elongation
Chaohua Cheng,Xuede Wang,Xiyuan Ni
Chinese Science Bulletin , 2005, DOI: 10.1007/BF02897515
Abstract: Lintless mutant is a super-short fiber mutant in upland cotton only 4–8 mm in fiber length and also named Ligon cotton controlled by one dominant geneLi 1. Fiber ultrastructure of the mutant (Li 1) and its wild type (li 1)in situ andin vitro was observed under an electron microscope to understand its cytological characteristics during the fiber cell elongation. The results showed that the mutant fiberin situ had thinner cytoplasm, more small vacuoles, less mitochondria, Gllgi apparatus and endoplasmic reticula, and there were more starch granules which were free or packed in the amyloplast beside the cell wall than that of wild type. It was indicated that scarcity of functional organelles and disability of transformation from starch to sugar might be associated with the fact that the mutant fiber cell was aborted too early to elongate into normal length. Mutant ovule in some media containing GA3 could produce a kind of huge callus that grew faster than normal ovules. The callus was covered with many white, loose, and semitransparent fiber-like cells that apt to get off from ovule. These fiber-like cells were multicellular fibers generated by cell division and had black dots just like pigment glands in the stem and leaf of cotton. There were lots of micro-tubes beside cytoplasm membrane of the multicellular fiber, which were thought to be primary preparation for second wall deposition of multicellular fiber. It was indicated that GA3 might induce the expression of gene(s) that kept inactive in the field condition and then stimulate the original fiber cellin vitro to undergo division again.
Effect of hydroxide radical and surfactant on photodegradation of acetochlor
游离羟基及表面活性剂对乙草胺光解的影响

HUA Rimao,XU Li,YUE Yongde,TANG Feng,LI Xuede,CAO Haiqun,WU Xiangwei,
花日茂
,徐利,岳永德,汤锋,李学德,操海群,吴祥为

环境科学学报 , 2006,
Abstract: 以高压汞灯为光源,以p-亚硝基-N,N-二甲基苯胺(PNDA)作为·OH自由基的探针,研究了H2O2及表面活性剂0206-B(苯乙烯苯酚聚氧乙烯醚和十二烷基苯磺酸钙混剂)对除草剂乙草胺在水中间接光解作用的影响.结果表明,H2O2对水中乙草胺具有光敏化降解作用,其敏化作用效应与·OH有关;而0206-B对乙草胺具有光猝灭降解作用,光猝灭降解作用效应与0206-B减少水溶液中的·OH自由基含量有关.乙草胺直接光解与H2O2作用的乙草胺间接光解相同的产物有Rf(比移值)为0.12、0.52、0.61、0.72的化合物;H2O2敏化降解抑制了乙草胺直接光解产物Rf为0.04、0.10、0.18、0.21、0.79的化合物产生,但促进乙草胺间接光解Rf为0.45、0.66的新光解产物生成.
Photochemical degradation of chlorpyrifos in water
毒死蜱在水溶液中的光化学降解

WU Xiangwei,NUA Rimao,TANG Feng,LI Xuede,CAO Haiqun,YUE Yongde,
吴祥为
,花日茂,汤锋,李学德,操海群,岳永德

应用生态学报 , 2006,
Abstract: In this paper, the effects of different light sources, temperature, pH, and water quality on the photochemical degradation of clilorpyrifos in water were examined under natural and simulated solar irradiation. The results showed that the photochemical degradation of chlorpyrifos in water followed the first order reaction, and its half-life was 0.62, 6.92, 19.74 and 22.50 h under high pressure mercury lamp (HPML), xenon lamp (XL), ultraviolet lamp (UV), and sunlight (SL) irradiation, respectively. Temperature had a significant effect on the degradation rate of chlorpyrifos, which was increased with increasing temperature and reached the maximum at 35 degrees C. The degradation rate of chlorpyrifos was stable both in acid and in neutral buffer solution, but enhanced in alkaline buffer solution. Water quality also had a significant effect, with a decreasing degradation rate of chlorpyrifos in the sequence of distilled water > tap water > river water > lake wate > paddy water.
Isolation, identification and degradation-efficiency measurement of chlorpyrifos degradaing bacteria
毒死蜱降解菌的分离鉴定与降解效能测定

WU Xiangwei,HUA Rimao,CAO Haiqun,TANG Feng,LI Xuede,YUE Yongde,
吴祥为
,花日茂,操海群,汤锋,李学德,岳永德

环境科学学报 , 2006,
Abstract: 取毒死蜱废水处理系统出口处的污泥进行驯化培养,分离出能降解毒死蜱的3株高效降解菌株B、D1和D3,对降解效果最好的D3菌株经中科院微生物研究所鉴定为玫瑰红红球菌(Rhodococcus rhodochrous);3株菌株的生长情况及对毒死蜱的降解动力学研究表明,B菌株在第3天繁殖增量达到最大,D1、D3菌株在第4天繁殖增量达到最大,B、D1和D3菌株的最适宜生长温度都是在30℃;采用10 mg·L-1毒死蜱作为唯一碳源时,B、D1和D3菌株对毒死蜱的降解速率分别为0.0543、0.0479和0.0620 h-1;对于浓度为10 mg·L-1的毒死蜱,D3菌投入的初始菌量OD223为0.4是最适宜的;D3菌对不同初始浓度的毒死蜱降解表明,初始浓度增大,降解速率降低,半衰期延长.
Observation of fiber ultrastructure of Ligon lintless mutant in upland cotton during fiber elongation
Chaohua Cheng,Xuede Wang,Xiyuan Ni,
CHENGChaohua
,WANGXuede,NIXiyuan

科学通报(英文版) , 2005,
Abstract: Lintless mutant is a super-short fiber mutant in upland cotton only 4–8 mm in fiber length and also named Ligon cotton controlled by one dominant geneLi 1. Fiber ultrastructure of the mutant (Li 1) and its wild type (li 1)in situ andin vitro was observed under an electron microscope to understand its cytological characteristics during the fiber cell elongation. The results showed that the mutant fiberin situ had thinner cytoplasm, more small vacuoles, less mitochondria, Gllgi apparatus and endoplasmic reticula, and there were more starch granules which were free or packed in the amyloplast beside the cell wall than that of wild type. It was indicated that scarcity of functional organelles and disability of transformation from starch to sugar might be associated with the fact that the mutant fiber cell was aborted too early to elongate into normal length. Mutant ovule in some media containing GA3 could produce a kind of huge callus that grew faster than normal ovules. The callus was covered with many white, loose, and semitransparent fiber-like cells that apt to get off from ovule. These fiber-like cells were multicellular fibers generated by cell division and had black dots just like pigment glands in the stem and leaf of cotton. There were lots of micro-tubes beside cytoplasm membrane of the multicellular fiber, which were thought to be primary preparation for second wall deposition of multicellular fiber. It was indicated that GA3 might induce the expression of gene(s) that kept inactive in the field condition and then stimulate the original fiber cellin vitro to undergo division again.
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