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Search Results: 1 - 10 of 1611 matches for " LEONOR; RUIBAL BRUNET "
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Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
CRUZ SUI,OTTO; PéREZ GUEVARA,MARíA TERESA; IZQUIERDO MáRQUEZ,MARICELA; LOBAINA BATELEMY,LEONOR; RUIBAL BRUNET,IGNACIO; SILVA CABRERA,ELADIO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it is reported the evaluation of the davih-blot system (davih laboratories, cuba) against the proficiency panels sent to the national aids reference laboratory by the world health organization (who) in order to ensure the quality in the diagnosis of hiv antibodies during 1989, 1991 and 1993. the system is also compared with those of the dupont and diagnostic pasteur firms in a group of 467 positive sera by elisa. on studying the who's samples, the system proved to be highly sensitive and specific. in the comparison made with the hiv-1 western blot igg version 1.2 (dupont company, usa) and with the lav-blot 1 (diagnostic pasteur, france), there was a coincidence of 100 and 97 %, respectively.
Fracaso del tratamiento antirretroviral y terapias de salvamento: Revisión actualizada
Díaz Torres,Héctor Manuel; Ruibal Brunet,Ignacio; Sánchez Ruiz,Julieta;
Revista Cubana de Medicina , 2003,
Abstract: those discoveries that are milestones in the search for an effective therapeutics against hiv/aids are stressed and it is reminded that an efficient vaccine has not been developed yet and that it has not been possible to attain the limited objective of turning that infection into a chronic and manageable condition at a large scale. the strategies of antiretroviral therapy, the definition and causes of the therapeutic failure, and the role of the hiv resistance to the antiretroviral agents as a cause of the failure of therapy are briefly reviewed. reference is made to the results of the research on the prevalence of different mutations granting resistance in our country. the implications in relation to the antiretroviral therapeutics are also commented upon. the salvage therapies proposed at present are reviewed and their importance to face the challenges of medical assistance in patients with limited therapeutic options is assessed.
Inhibición de la replicación del virus de inmunodeficiencia humana por extractos de taninos de Pinus caribaea Morelet
Ignacio Juan Ruibal Brunet,Marta Dubed Echevarría,Francisco Martínez Luzardo,Enrique Noa Romero
Revista Cubana de Farmacia , 2003,
Abstract: Diferentes concentraciones de 6 extractos de corteza de Pinus caribaea Morelet var. caribaea se enfrentaron a 2 dosis de virus en un ensayo in vitro, sobre células MT4; la actividad antiviral se midió por ensayo inmunoenzimático de captura de proteína 24 del virus. Todas las fracciones mostraron actividad citotóxica moderada y solo una fue altamente tóxica. La fracción 02 mostró un alto porcentaje de inhibición de la replicación viral, en relación con la dosis viral y la concentración del producto, con un índice de selectividad de 100, pero son necesarios estudios adicionales sobre la identificación de la estructura química para definir el mecanismo de acción del producto. Six different fractions from the bark of Pinus caribae Moralet var. caribae were faced in five different concentrations against two viral doses (MOI 0,1 y 0,01) in a vitro assay on MT4 cell lines; the antiviral activity was measured by p24 Ag capture ELISA assay (DAVIH Agp24). All the fractions showed a mild cytotoxicity activity and only one fraction showed the highest cytotoxicity activity. The fraction 02 had the highest percentage of viral replication inhibition, correlated with the viral dose and the product concentration, having a selectivity rate of 100; however, more research about the chemical structure of active compounds, and possible mechanisms of action are needed.
Fracaso del tratamiento antirretroviral y terapias de salvamento: Revisión actualizada
Héctor Manuel Díaz Torres,Ignacio Ruibal Brunet,Julieta Sánchez Ruiz
Revista Cubana de Medicina , 2003,
Abstract: Se mencionaron los descubrimientos que constituyen hitos en la búsqueda de una terapéutica efectiva contra el VIH/SIDA y se recordó que aún no se ha desarrollado una vacuna eficaz ni se ha alcanzado a gran escala el limitado objetivo de convertir esa infección en una condición crónica y manejable. Se revisaron brevemente las estrategias de terapia antirretroviral, la definición y causas del fracaso terapéutico, el papel de la resistencia del VIH a los antirretrovirales como causa de fracaso de la terapia y se hizo referencia a resultados de investigación sobre la prevalencia de distintas mutaciones que confieren resistencia en nuestro país y se comentaron sus implicaciones en relación con la terapéutica antirretroviral. Se revisaron las terapias de salvamento que se proponen en la actualidad y se valoró la importancia de las mismas para enfrentar los retos de la asistencia médica en pacientes con opciones terapéuticas limitadas. Those discoveries that are milestones in the search for an effective therapeutics against HIV/AIDS are stressed and it is reminded that an efficient vaccine has not been developed yet and that it has not been possible to attain the limited objective of turning that infection into a chronic and manageable condition at a large scale. The strategies of antiretroviral therapy, the definition and causes of the therapeutic failure, and the role of the HIV resistance to the antiretroviral agents as a cause of the failure of therapy are briefly reviewed. Reference is made to the results of the research on the prevalence of different mutations granting resistance in our country. The implications in relation to the antiretroviral therapeutics are also commented upon. The salvage therapies proposed at present are reviewed and their importance to face the challenges of medical assistance in patients with limited therapeutic options is assessed.
Inactivacion de virus ADN envueltos en la producción de hemoderivados (Albúmina e Inmunoglobulinas)
Ignacio Juan Ruibal Brunet,Enrique Noa Romero,Regina Zonia Martín García,Nibaldo Luis González Sosa
Revista Cubana de Farmacia , 1999,
Abstract: Se estudió la inactivación del virus herpes simple humano tipo 1 como modelo de virus ADN envueltos, durante las etapas de producción de las inmunoglobulinas intramuscular e intravenosa y la albúmina humana, las etapas del método de fraccionamiento alcohólico para la obtención de estos productos, así como los métodos de remoción y/o inactivación introducidos en el proceso de manufactura, pasteurización y cromatografía de intercambio iónico. El virus se cuantificó por efecto citopático. La obtención de valores de reducción acumulativos reportados en este trabajo demuestran que el método de fraccionamiento alcohólico utilizado en Cuba como variante del método de Cohn-Oncley, combinando métodos de inactivación/remoción, produce un nivel de inactivación de virus ADN envueltos que garantiza una alta seguridad biológica de estos productos para su uso en humanos. Inactivation of human herpes simplex virus I asa model of enveloped DNA virus was studied in the stages of production of intramuscular/ intravenous inmunoglobulins and human albumin, in the phases of alcohol fractionation for obtaining these products, and in the methods of removal and/or inactivation implemented in manufacture, pasteurization and ion - exchange chromatography. The virus was quantified by cytophatic effects. The cumulative reduction values reported in this paper proved that the use of alcohol fractionation in Cuba, as a variant of Cohn - Oncley method, combined with removal/inactivation procedures resulted in enveloped DNA virus inactivation assuring high biological safety for albumin and immunoglobulins for their use in human beings.
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba
Ruibal-Brunet Ignacio J.,Cuevas M. Teresa,Díaz-Torres Héctor,Villahermosa M. Luisa
Revista Panamericana de Salud Pública , 2001,
Abstract: Objectives. To determine the prevalence of drug resistance and to analyze the subtyping in HIV-1 samples from Cuba. Methods. From an estimated total number of 1 950 HIV-1-infected persons in Cuba, a sample of 103 patients were studied, 76 of whom had received drug treatment for HIV and 27 who had not. The RNA plasma viral load was measured, and automated sequencing was used to assess resistance mutations to reverse transcriptase inhibitors (RTIs) and to protease inhibitors (PIs). Subtyping in the V3 region was performed using heteroduplex mobility assay (HMA). In order to corroborate the HMA results, sequencing of env (C2-V3-C3) was done with one-third of the samples in each of the subtype groups detected by HMA. Results. Out of the 103 samples, 81 of them (78.6%) were classified as subtype B, 19 (18.5%) as subtype A, and 3 (2.9%) as subtype C. The prevalence of resistance mutations was 26.2% to RTIs, none to PIs alone, and 3.9% to both categories of drugs. The prevalence of resistance to nucleoside RTIs (NRTIs) was 27.6% in treated patients and 7.4% in the untreated patients, and for nonnucleoside RTIs (NNRTIs) it was 5.3% and 0%, respectively. Among treated patients a low frequency (2.6%) of dual resistance to zidovudine (ZDV) plus lamivudine (3TC) and abacavir (ABC) was detected, and multidrug resistance to NRTIs was not found. In relation to PIs together with RTIs, the prevalence of resistance was 5.3% for treated patients and 0% for untreated patients. Conclusions. Even though Cuba is generally considered an area where subtype B is dominant, we detected a high proportion of non-B subtype viruses. The low prevalence of resistance mutations to RTIs and PIs reflects the delay in introducing these drugs to Cuba. Multidrug resistance to RTIs was not found, so, as of now, the use of these drugs continues to be an option for Cuban patients.
Inactivacion de virus ADN envueltos en la producción de hemoderivados (Albúmina e Inmunoglobulinas)
Ruibal Brunet,Ignacio Juan; Noa Romero,Enrique; Martín García,Regina Zonia; González Sosa,Nibaldo Luis; Ramírez Mas,Ana Teresa;
Revista Cubana de Farmacia , 1999,
Abstract: inactivation of human herpes simplex virus i asa model of enveloped dna virus was studied in the stages of production of intramuscular/ intravenous inmunoglobulins and human albumin, in the phases of alcohol fractionation for obtaining these products, and in the methods of removal and/or inactivation implemented in manufacture, pasteurization and ion - exchange chromatography. the virus was quantified by cytophatic effects. the cumulative reduction values reported in this paper proved that the use of alcohol fractionation in cuba, as a variant of cohn - oncley method, combined with removal/inactivation procedures resulted in enveloped dna virus inactivation assuring high biological safety for albumin and immunoglobulins for their use in human beings.
Inhibición de la replicación del virus de inmunodeficiencia humana por extractos de taninos de Pinus caribaea Morelet
Ruibal Brunet,Ignacio Juan; Dubed Echevarría,Marta; Martínez Luzardo,Francisco; Noa Romero,Enrique; Vargas Guerra,Luis M; Santana Romero,Jorge L;
Revista Cubana de Farmacia , 2003,
Abstract: six different fractions from the bark of pinus caribae moralet var. caribae were faced in five different concentrations against two viral doses (moi 0,1 y 0,01) in a vitro assay on mt4 cell lines; the antiviral activity was measured by p24 ag capture elisa assay (davih agp24). all the fractions showed a mild cytotoxicity activity and only one fraction showed the highest cytotoxicity activity. the fraction 02 had the highest percentage of viral replication inhibition, correlated with the viral dose and the product concentration, having a selectivity rate of 100; however, more research about the chemical structure of active compounds, and possible mechanisms of action are needed.
Genotypic resistance mutations to antiretroviral drugs in HIV-1 B and non-B subtypes from Cuba
Ruibal-Brunet,Ignacio J.; Cuevas,M. Teresa; Díaz-Torres,Héctor; Villahermosa,M. Luisa; Noa-Romero,Enrique; Vázquez de Parga,Elena; Blanco de Armas,Madelin; Pérez-álvarez,Lucía;
Revista Panamericana de Salud Pública , 2001, DOI: 10.1590/S1020-49892001000900005
Abstract: objectives. to determine the prevalence of drug resistance and to analyze the subtyping in hiv-1 samples from cuba. methods. from an estimated total number of 1 950 hiv-1-infected persons in cuba, a sample of 103 patients were studied, 76 of whom had received drug treatment for hiv and 27 who had not. the rna plasma viral load was measured, and automated sequencing was used to assess resistance mutations to reverse transcriptase inhibitors (rtis) and to protease inhibitors (pis). subtyping in the v3 region was performed using heteroduplex mobility assay (hma). in order to corroborate the hma results, sequencing of env (c2-v3-c3) was done with one-third of the samples in each of the subtype groups detected by hma. results. out of the 103 samples, 81 of them (78.6%) were classified as subtype b, 19 (18.5%) as subtype a, and 3 (2.9%) as subtype c. the prevalence of resistance mutations was 26.2% to rtis, none to pis alone, and 3.9% to both categories of drugs. the prevalence of resistance to nucleoside rtis (nrtis) was 27.6% in treated patients and 7.4% in the untreated patients, and for nonnucleoside rtis (nnrtis) it was 5.3% and 0%, respectively. among treated patients a low frequency (2.6%) of dual resistance to zidovudine (zdv) plus lamivudine (3tc) and abacavir (abc) was detected, and multidrug resistance to nrtis was not found. in relation to pis together with rtis, the prevalence of resistance was 5.3% for treated patients and 0% for untreated patients. conclusions. even though cuba is generally considered an area where subtype b is dominant, we detected a high proportion of non-b subtype viruses. the low prevalence of resistance mutations to rtis and pis reflects the delay in introducing these drugs to cuba. multidrug resistance to rtis was not found, so, as of now, the use of these drugs continues to be an option for cuban patients.
La sociedad civil en el proceso de reformas a la Corte Suprema Argentina
Ruibal, Alba M.;
Revista mexicana de sociología , 2008,
Abstract: this article analyzes the role of civil society and its interaction with government actors in the process of the reform of the argentine supreme court initiated in 2003. it explains that social mobilization and the action of ngos were determining factors in the configuration of incentives for the executive branch to initiate this process of reform, as well as in the content and form of the changes made.
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