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Search Results: 1 - 10 of 12915 matches for " LEONOR; CRUZ SUI "
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Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
OTTO CRUZ SUI,MARíA TERESA PéREZ GUEVARA,MARICELA IZQUIERDO MáRQUEZ,LEONOR LOBAINA BATELEMY
Revista Cubana de Medicina Tropical , 1997,
Abstract: Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los a os 1989, 1991 y 1993, así como la comparación del sistema con los de las firmas DuPont y Diagnostic Pasteur en un grupo de 467 sueros positivos por ELISA. En el estudio de las muestras de la OMS el sistema demostró ser altamente sensible y específico. Al compararlos con los estuches HIV-1 Western blot IgG versión 1.2 (DuPont Company, EE.UU.) y New LAV-blot 1 (Diagnostic Pasteur, Francia) tuvo una coincidencia del 100 y 97 %, respectivamente. It is reported the evaluation of the DAVIH-BLOT system (DAVIH Laboratories, Cuba) against the proficiency panels sent to the National AIDS Reference Laboratory by the World Health Organization (WHO) in order to ensure the quality in the diagnosis of HIV antibodies during 1989, 1991 and 1993. The system is also compared with those of the Dupont and Diagnostic Pasteur firms in a group of 467 positive sera by ELISA. On studying the WHO's samples, the system proved to be highly sensitive and specific. In the comparison made with the HIV-1 Western Blot IgG version 1.2 (Dupont company, USA) and with the LAV-blot 1 (Diagnostic Pasteur, France), there was a coincidence of 100 and 97 %, respectively.
Pesquisaje de anticuerpos contra el virus linfotrópico de células T humanas tipo I (HTLV-I) en donantes de sangre y grupos de riesgo
SILVA CABRERA,ELADIO; PéREZ GUEVARA,MARíA T.; LUBIáN CABALLERO,ANA L.; DE LA FUENTE ARZOLA,JOSé L.; NAVEA LEYVA,LEONOR; CRUZ SUI,OTTO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it was studied the presence of htlv-i antibodies in 3 774 sera, 1 409 of them were from blood donors, 1 444 were from patients who had suffered from some sexually transmitted disease recently, and 921 from transfused patients. the davih htlv i (elisa) and davih blot htlv i (western blot) systems produced at the davih laboratories (havana, cuba) were used for screening and confirmation, respectively. of the 68 reactive sera utilized in the elisa test, it was confirmed the presence of htlv i/ii antibodies in 2, whereas 12 were considered as undetermined by the western blot.
Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
CRUZ SUI,OTTO; PéREZ GUEVARA,MARíA TERESA; IZQUIERDO MáRQUEZ,MARICELA; LOBAINA BATELEMY,LEONOR; RUIBAL BRUNET,IGNACIO; SILVA CABRERA,ELADIO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it is reported the evaluation of the davih-blot system (davih laboratories, cuba) against the proficiency panels sent to the national aids reference laboratory by the world health organization (who) in order to ensure the quality in the diagnosis of hiv antibodies during 1989, 1991 and 1993. the system is also compared with those of the dupont and diagnostic pasteur firms in a group of 467 positive sera by elisa. on studying the who's samples, the system proved to be highly sensitive and specific. in the comparison made with the hiv-1 western blot igg version 1.2 (dupont company, usa) and with the lav-blot 1 (diagnostic pasteur, france), there was a coincidence of 100 and 97 %, respectively.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Lubián Caballero,Ana Luisa; Díaz Torres,Héctor Manuel; Silva Cabrera,Eladio; Pérez Guevara,María Teresa; Cruz Sui,Otto; de la Fuente Arzola,José Luis; Navea Leyva,Leonor; Noa Romero,Enrique;
Revista Cubana de Medicina , 1998,
Abstract: in the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human t-lymphotropic virus type i (htlv-i), and also as a continuation of researches carried out between 1989 and 1990. the elisa davih-htlv-i system was used for the antibodies investigation, and as a certifying test, the western blot davih-blot htlv-i was used too, both from the cuban davih laboratories. the presence of anti htlv-i antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. the seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the caribbean area.
A new bacterial disease of carnation in Portugal caused by Burkholderia andropogonis
Eloy,Madalena; Cruz,Leonor;
Revista de Ciências Agrárias , 2008,
Abstract: the occurrence of a leaf spot disease of carnation caused by burkholderia andropogonis is recorded for the first time in portugal. symptoms consisted of ?eyespot? lesions on all aerial plant parts, often bordered by water-soaked halos on the leaves. as the disease progressed lesions became dark brown and affected areas dried out. phenotypic studies and polymerase chain reaction using specific primers pf/pr targeted to 16s rdna of b. andropogonis were used to identify the pathogen. pathogenicity tests on china pink plants, re-isolation of the pathogen from inoculated plants and further pcr testing confirmed the identification of the bacterium. infected plants came from an open air nursery and the whole production was destroyed to avoid dissemination of the pathogen.
A new bacterial disease of carnation in Portugal caused by Burkholderia andropogonis
Madalena Eloy,Leonor Cruz
Revista de Ciências Agrárias , 2008,
Abstract: The occurrence of a leaf spot disease of carnation caused by Burkholderia andropogonis is recorded for the first time in Portugal. Symptoms consisted of ‘eyespot’ lesions on all aerial plant parts, often bordered by water-soaked halos on the leaves. As the disease progressed lesions became dark brown and affected areas dried out. Phenotypic studies and Polymerase Chain Reaction using specific primers Pf/Pr targeted to 16S rDNA of B. andropogonis were used to identify the pathogen. Pathogenicity tests on china pink plants, re-isolation of the pathogen from inoculated plants and further PCR testing confirmed the identification of the bacterium. Infected plants came from an open air nursery and the whole production was destroyed to avoid dissemination of the pathogen. A ocorrência da mancha bacteriana do craveiro causada por Burkholderia andropogonis é pela primeira vez assinalada em Portugal. Os sintomas observados consistiam em manchas em forma de olho-de-perdiz em todos os órg os aéreos das plantas afectadas, frequentemente circundadas por halos hidrópicos nas folhas. à medida que a doen a progredia, as les es adquiriam uma colora o castanha escura, acabando os órg os afectados por secar. A identifica o do agente causal da doen a baseou-se no estudo dos seus caracteres fenotípicos e na Reac o em Cadeia da Polimerase (PCR), utilizando os iniciadores específicos Pf/Pr dirigidos à regi o 16S rDNA de B. andropogonis. A identifica o foi confirmada por ensaios de patogenicidade em cravinas, reisolamento do agente causal da doen a a partir das plantas inoculadas e novos ensaios PCR. As plantas infectadas provinham de um viveiro ao ar livre e toda a produ o foi destruída a fim de evitar a dissemina o do patogéneo.
Política de dividendos e ciclo de vida das empresas
Salsa,Maria Leonor Cruz Reis;
Revista Encontros Científicos - Tourism & Management Studies , 2010,
Abstract: dividends are considered one of the most important business financial decisions. some theories like agency costs or asymmetric information and signalization have contributed with theoretical arguments or financial determinants to explain the level of dividends distributed by firms. this study tests the impact of the life cycle of the firm, and phases that it goes through, on its dividend policy. it also tests some financial determinants that the above mentioned theories demonstrate to be significant: return on investment, dimension, risk, number of shareholders, annual growth rate of net income, investment opportunities, firm?s debt financing, growth rate of sales and dividend payout of the year before. the empirical study is based on a sample of european firms from 6 countries: germany, denmark, finland, united kingdom, italy and sweden. the results do not confirm the life cycle?s hypotheses, showing that firms do not pay necessarily more dividends in their maturity phase. the results validate other hypotheses and reinforce some financial determinants and also reveal some similarities and differences among the dividend policies of the six countries.
Prevalência da hipovitaminose A em pré-escolares no Estado de Sergipe, 1998
Martins,Maisa Cruz; Santos,Leonor Maria Pacheco; Assis,Ana Marlúcia Oliveira;
Revista de Saúde Pública , 2004, DOI: 10.1590/S0034-89102004000400009
Abstract: objective: to determine the prevalence of vitamin a deficiency in a population-based sample. methods: this was a cross-sectional study conducted in the state of sergipe, northeastern brazil, in may and june, 1998. it involved 607 children aged 6 to 60 months. the information was obtained by means of interviews with the persons responsible for these children, in their homes. blood samples were collected via venous puncture and serum retinol assays were carried out by means of high performance liquid chromatography (hplc). in simultaneous analyses of the study variables, p<0.05 was accepted for testing statistically significant associations. results: a mean serum retinol value of 0.87 μmol/l (±0.38) was found among the children investigated. the prevalence of levels considered low (0.35 to 0.69 μmol/l) was 22.5% and the prevalence of levels considered deficient (<0.35 μmol/l) was 9.6%. inadequate serum retinol levels were therefore seen in 32.1% of the children. the serum retinol level showed an association with per capita family income and the weight/age indicator. no statistically significant association was found for serum retinol level in relation to the child's age and sex, or the mother's variables. conclusion: vitamin a deficiency among preschool children is an important public health problem. hypovitaminosis a is mainly related to low per capita family income and low infant weight.
Panorama das a??es de controle da deficiência de vitamina A no Brasil
Martins, Maisa Cruz;Oliveira, Yedda Paschoal de;Coitinho, Denise Costa;Santos, Leonor Maria Pacheco;
Revista de Nutri??o , 2007, DOI: 10.1590/S1415-52732007000100001
Abstract: objective: this manuscript aims to describe the implementation of actions to control vitamin a deficiency in brazil. methods: the study was based on secondary data obtained from official reports and technical documents from the former instituto nacional de alimenta??o e nutri??o (inactivated in 1997), from the coordena??o geral da política nacional de alimenta??o e nutri??o, ministry of health and information obtained from key informants employing the multidimensional approach based on the triad: structure-process-result. results: brazil was pioneer in taking the initiative of linking vitamin a distribution to the national immunization days, a strategy that later on was recommended by the world health organization and the united nations children's fund. since 1983, vitamin a megadoses are employed as a strategy to fight this micronutrient deficiency by the ministério da saúde. coverage of the target population (children 6-59 months old) is steadily increasing, reaching 72% and 68% in the years 2002 and 2003 respectively. these coverage rates are similar to those reported in the 40 countries that have also adopted this strategy. since 2001 the program was extended to lactating women, in the immediate post-partum period. conclusion: the program achievements until 1992 were very modest, fact that could be explained by the irregularity of vitamin a capsule distribution. from 1994 on the distribution was more constant, but the need to promote more integrated actions in the health services, as well as far reaching social actions, beyond the routine distribution of vitamin a capsules, still remains.
Evaluación de la inmovilización de un péptido de gp36 de VIH-2 en membranas de nitrocelulosa
Díaz Herrera,Dervel Felipe; Cruz Sui,Otto; Montano Tamayo,Lucy; Silva Cabrera,Eladio;
Revista Cubana de Medicina Tropical , 2011,
Abstract: introduction: antigen immobilization in solid supports is used for the development of several immunoassays. one of the first technologies developed was the protein adsorption by direct application to nitrocellulose. objective: to standardize the immobilization of a synthetic peptide of the hiv-2 transmembrane protein gp36 to nitrocellulose support for diagnostic purposes and to evaluate the performance parameters in a group of serum samples with recognized interesting reactivity. methods: the peptide was freely immobilized, conjugated to bovine serum albumin (bsa) and to keyhole limpet hemocyanin (klh) as carrier proteins. immobilization parameters were analyzed and then, the optimal immobilization alternative was determined. using the chosen variant, the diagnostic sensitivity and specificity against reference panels of the aids research laboratory were evaluated. analytical specificity was evaluated with reactive samples to hiv-1 and htlv-i. results: the analysis of the immobilized peptide variants to nitrocellulose membranes showed that the gp36 peptide-bsa was the one that succeeded in setting the greatest differentiation between positive and negative samples. there were observed 100% sensitivity, 95.2% diagnostic specificity and 100% analytical specificity. conclusions: the gp36-bsa peptide immobilized on nitrocellulose membranes showed efficacy for the serological diagnosis of hiv-2, which will allow considering this peptide for diagnostic uses in systems with nitrocellulose based solid phase.
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