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Search Results: 1 - 10 of 368972 matches for " L. P. Moles-Cervantes "
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Presence of Zoonotic Interest Bacteria in Slaughter Pigs
L. Hernandez-Andrade,P. Elizalde-Castaneda,R.M. Urrutia,L.P. Moles-Cervantes,E. Diaz-Aparicio
Journal of Animal and Veterinary Advances , 2012,
Abstract: The objective of this study was to recognize, the presence of Salmonella, Brucella, Leptospira and Yersinia in slaughter swine that are apparently healthy. Samples were taken from blood, tonsils, ileum, kidney and spleen of 100 pigs that came from the States of Jalisco, Guanajuato and Sonora. For brucellosis diagnosis, card test and bacteriology from spleen were performed. For leptospirosis a micro agglutination technique was used and bacteriology from kidney. Bacteriology study was performed for Salmonella diagnosis using samples from ileum and tonsil samples were used for diagnosis of Yersinia enterocolitica. Salmonella study results were negative for all animals. Five reactors were found for Brucella by serology (5%), but no isolation was achieved. Twenty-five percent positive reactors to two serovars of Leptospira were found: Bratislava 19 animals (19%) and Panama 6 (6%) with no isolation of the bacteria. Twenty-two percent isolations of Yersinia enterocolitica biotype 1 serotypes 0:3 and 0:9 were obtained. Positive animals came from the States of Jalisco and Guanajuato. It is concluded that of the 4 diseases that were studied, the one that had more relevance was Y. enterocolitica with 22% of isolations in slaughter swine that are apparently healthy.
Leptospirosis Seroconversion During an Abortion Outbreak in a Mexican Hairless Swine Herd
M. A. Cisneros-Puebla,D. Mota-Rojas,L. P. Moles-Cervantes,R.Ram?rez-Necoechea,M. Alonso-Spilsbury
Journal of Animal and Veterinary Advances , 2012,
Abstract: A herd of Mexican Hairless Swine, maintained in an agro-silvi-culture pasture in the village of Chapa de Mota, Mexico, underwent a serological study after one of the sows aborted a litter showing fever. Blood samples were drawn from thirty-four animals in reproductive stage, which then underwent a treatment, based on a streptomycin dose of 25 mg/kg live weight during five days. Twenty-eight samples were taken 15 days after the herd either farrowed or aborted. Seroconversion was found in five animals with a serovar of hardjo H 89 origin. One of the animals aborted after seventy-eight days of gestation; in all the cases there was a negative increase of between 1:200 to 1:800 titres, while the serovar bratislava presented titres in six animals. In two cases the seroconversion went from a negative to a 1:200, while one of the pigs miscarried on the 77th day of pregnancy, and two other animals went from a titre 1:100 to 1:200. Another sow lost a litter on day 84, and in the last two animals the titre was maintained at 1:100, though one of the pigs aborted on the 86th day. This paper constitutes the first report of an outbreak of abortions in the Mexican Hairless Swine caused by Leptospira interrogans serovars bratislava and hardjo.
Canine Leptospirosis Serology in Southern Mexico City  [PDF]
Ignacio Martínez-Barbabosa, Edubiel Arturo Alpizar-Sosa, Dolores Guadalupe Gavaldón-Rosas, Luís Pedro Moles-Cervantes, Marcia Gutiérrez Cárdenas, Rafael García-González, Michael Shea, Ana María Fernández-Presas
Open Journal of Medical Microbiology (OJMM) , 2016, DOI: 10.4236/ojmm.2016.64022
Abstract: Background: Leptospirosis is an important zooanthroponotic disease spread worldwide which infection is recognized as a re-emergent disease. Leptospirosis is a systemic disease of humans and domestic animals, mainly dogs, cattle and swine, characterized by fever, renal and hepatic insufficiency, pulmonary manifestations and reproductive failure. Objective: To study the seroprevalence of anti-Leptospira antibodies in an urban canine population. Materials and Methods: The study was performed in March 2014 in stray dogs that had been captured in the districts of Coyoacan, Iztapalapa, Iztacalco and Benito Juarez in Mexico City; 117 dogs were analyzed, from each of which 5 ml of blood were obtained. Serology was performed using a MAT test (Microscopic Agglutination Test), finding that the antigens corresponded to 13 serotypes of Leptospirainterrogans. Three age groups were formed: Group 1) younger than a year (n = 28), Group 2) 1 to 6 years (n = 75), and Group 3) older than 6 years (n = 14). Results: Of the analyzed sera, 28.2% were seropositive to one or more Leptospira serotypes, 74% of the positive seracoagglutinated with two or more serotypes. The most frequent serotypes were: Canicola, Icterohaemorrhagiae and Portland-vere. Serotypes Canicola,
Evaluación de una bacterina homóloga contra la leptospirosis bovina
Orozco Vega,Laura Elena; López Flores,Rafael; Moles y Cervantes,Luis Pedro; Quiroz Valiente,Jorge;
Revista Cubana de Medicina Tropical , 2005,
Abstract: 48 adult bovine females dividided into 6 groups were used aimed at characterizing the immune response induced in breastfeeeding cows by an homologous bacterin formulated with different adjuvants. they were intramuscularly administered 2 milliliters of a bacterin formulated with leptospira interrogans serovars uam, wolffi, hardjo, bratislava, grippotyphosa and panama added with different adjuvants, such as aluminum hydroxide, freud’s complete adjuvant, freud’s incomplete adjuvant, liposoluble vitamins, bacterin plus disparasitization with levamisol. the control group was administred only with bacterin. immunization took place in 2 occasions at a time interval of 28 days. blood samples were taken every 7 days during the first month after vaccination, and every 28 days for the next 8 months. all the sera were analyzed by the microscopic agglutination test. the results were transformed into log10 and they were analyzed by nlin and glm of sas. the period of greater response was estimated by the prediction model (wood). the bacterin did not produce alteration either in the physiological constants, or in milk production. the serovars of leptospira interrogans that induced higher titers were uam, hardjo and wolffi. the statistical difference between treatments and between serovars was determined.
Evaluación de una bacterina homóloga contra la leptospirosis bovina Evaluation of an homologous bacterin against bovine Leptospirosis
Laura Elena Orozco Vega,Rafael López Flores,Luis Pedro Moles y Cervantes,Jorge Quiroz Valiente
Revista Cubana de Medicina Tropical , 2005,
Abstract: Se utilizaron 48 hembras bovinas adultas, asignadas a 6 grupos, con el objetivo de caracterizar la respuesta inmune inducida en vacas lactantes por una bacterina homóloga formulada con diferentes adyuvantes. Se les administró por vía intramuscular 2 mL de la bacterina formulada con Leptospira interrogans serovares uam, wolffi, hardjo, bratislava, grippotyphosa y panama adicionada con diferentes adyuvantes como: hidróxido de aluminio, adyuvante completo de Freud, adyuvante incompleto de Freud, vitaminas liposolubles, bacterina más desparasitación con levamisol. Al grupo control solo se le administró bacterina. La inmunización se realizó en 2 ocasiones con diferencia de 28 d. Cada 7 d durante el primer mes posvacunación y posteriormente cada 28 d durante 8 meses se realizaron muestreos sanguíneos. Los sueros se analizaron por aglutinación microscópica. Los resultados se transformaron a Log10 y se analizaron por medio de los procedimientos para modelos no lineales y modelos lineales generales del programa estadístico SAS. Mediante el modelo de predicción (Wood) se estimó el período de mayor respuesta. La bacterina no produjo alteración en las constantes fisiológicas, ni en producción láctea. Los serovares de Leptospira interrogans que indujeron mayores títulos fueron uam, hardjo y wolffi. Se determinó diferencia estadística entre tratamientos y entre serovariedades. 48 adult bovine females dividided into 6 groups were used aimed at characterizing the immune response induced in breastfeeeding cows by an homologous bacterin formulated with different adjuvants. They were intramuscularly administered 2 milliliters of a bacterin formulated with Leptospira interrogans serovars uam, wolffi, hardjo, bratislava, grippotyphosa and panama added with different adjuvants, such as aluminum hydroxide, Freud’s complete adjuvant, Freud’s incomplete adjuvant, liposoluble vitamins, bacterin plus disparasitization with levamisol. The control group was administred only with bacterin. Immunization took place in 2 occasions at a time interval of 28 days. Blood samples were taken every 7 days during the first month after vaccination, and every 28 days for the next 8 months. All the sera were analyzed by the microscopic agglutination test. The results were transformed into Log10 and they were analyzed by NLIN and GLM of SAS. The period of greater response was estimated by the prediction model (Wood). The bacterin did not produce alteration either in the physiological constants, or in milk production. The serovars of Leptospira interrogans that induced higher titers were uam, hardj
Isotropization of Bianchi-Type Cosmological Solutions in Brans-Dicke Theory
P. Chauvet,J. L. Cervantes-Cota
Physics , 1995, DOI: 10.1103/PhysRevD.52.3416
Abstract: The cosmic, general analitic solutions of the Brans--Dicke Theory for the flat space of homogeneous and isotropic models containing perfect, barotropic, fluids are seen to belong to a wider class of solutions --which includes cosmological models with the open and the closed spaces of the Friedmann--Robertson--Walker metric, as well as solutions for models with homogeneous but anisotropic spaces corresponding to the Bianchi--Type metric clasification-- when all these solutions are expressed in terms of reduced variables. The existence of such a class lies in the fact that the scalar field, $\phi$, times a function of the mean scale factor or ``volume element'', $a^3 = a_1 a_2 a_3$, which depends on time and on the barotropic index of the equation of state used, can be written as a function of a ``cosmic time'' reduced in terms of another function of the mean scale factor depending itself again on the barotropic index but independent of the metrics here employed. This reduction procedure permites one to analyze if explicitly given anisotropic cosmological solutions ``isotropize'' in the course of their time evolution. For if so can happen, it could be claimed that there exists a subclass of solutions that is stable under anisotropic perturbations.
Hepatotoxicidad por propafenona
E. P. Martín,J. L. Cervantes,J. Yangüela
Revista Espa?ola de Enfermedades Digestivas , 2004,
Abstract:
Can induced gravity isotropize Bianchi I, V, or IX Universes?
J. L. Cervantes-Cota,P. Ag. Chauvet
Physics , 1998, DOI: 10.1103/PhysRevD.59.043501
Abstract: We analyze if Bianchi I, V, and IX models in the Induced Gravity (IG) theory can evolve to a Friedmann--Roberson--Walker (FRW) expansion due to the non--minimal coupling of gravity and the scalar field. The analytical results that we found for the Brans-Dicke (BD) theory are now applied to the IG theory which has $\omega \ll 1$ ($\omega$ being the square ratio of the Higgs to Planck mass) in a cosmological era in which the IG--potential is not significant. We find that the isotropization mechanism crucially depends on the value of $\omega$. Its smallness also permits inflationary solutions. For the Bianch V model inflation due to the Higgs potential takes place afterwads, and subsequently the spontaneous symmetry breaking (SSB) ends with an effective FRW evolution. The ordinary tests of successful cosmology are well satisfied.
LA LEPTOSPIROSIS CANINA Y SU PROBLEMáTICA EN MéXICO
Luna,A.M.A; Moles,C.L.P; Gavaldón,R.D; Nava,V.C; Salazar,G.F;
Revista de Salud Animal , 2008,
Abstract: leptospirosis in dogs is a disease that can have subclinical, acute or chronic presentation. its diagnosis is difficult due to the multiple clinical aspects and not always jaundice is present. its symptoms can be sub-clinical; therefore, the serological findings are more frequent than the symptoms of the disease. when the disease shows signs, it can cause death due to hepatic disorders and nephritis. the dogs that survive are carriers of the bacteria through their urine which makes them very risky and an important link in the epidemic chain of leptospirosis in man. the current situation of canine leptospirosis in mexico is not well known so it is convenient to make a literature review of it.
Enzymes of Entomopathogenic Fungi, Advances and Insights  [PDF]
Lluvia de Carolina Sánchez-Pérez, Juan Esteban Barranco-Florido, Silvia Rodríguez-Navarro, José Francisco Cervantes-Mayagoitia, Miguel ángel Ramos-López
Advances in Enzyme Research (AER) , 2014, DOI: 10.4236/aer.2014.22007
Abstract: Entomopathogenic fungi (EF) are recognized biological control agents of insects. Basically, the entomopathogenic fungi pathogen activity depends on the ability of its enzymatic equipment, consisting of lipases, proteases and chitinases, which are in charge of breaking down the insect’s integument. Lipases are the first enzymes synthesized by the entomopathogenic fungi. Recently, a cytochrome P450 subfamily, referred as CYP52XI and MrCYP52 has been identified in Beauveria bassiana and Metarhizium robertsii, respectively. These break down long-chain alkenes and fatty acids to become initial nutrients. Subsequently, subtilisin type (Pr1) proteases sintetize; these enzymes are considered as virulence indicators and they are regulated by a signal transduction mechanism activated by the protein kinase A (PKA) mediated by AMPc. Through the employment of genetic engineering, it has been possible to increase virulence producing Pr1 recombinants with Androctonus australis neurotoxins or with chitinases, reducing the insect’s time of death. In the course of time, the Pr1 protease gene has presented evolutionary adaptations by gene duplication or horizontal transfer infecting different orders of insects. In the same way, the entomopathogenic fungi chitinases have presented a functional diversification. Currently, these have been phylogenetically classified into three subgroups, in accordance to the catalytic site domain and the chitin binding domain. The chitinolytic activity has increased through a directed evolution processes and genetic recombination with Bombyx mori chitinase. Recently, enzymes have been employed as control agents for insects and phytopathogenic fungi (disease originator) opening new potentialities in order to improve the entomopathogenic fungi use. Solid state fermentation is a bioprocess that would produce at great scale enzymes and some other metabolites in grade of increasing the entomopathogenic fungi virulence, in the control of insects and potentially in some diseases affecting plants.
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