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Search Results: 1 - 10 of 208393 matches for " L. Alberghini "
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QUANTITATIVE ASSESSMENT OF CAMPYLOBACTER SPP. ON POULTRY CARCASSES
L. Alberghini,G. Colavita,V. Giaccone
Italian Journal of Food Safety , 2011, DOI: 10.4081/ijfs.2011.1s.39
Abstract: Campylobacter spp. are bacterial pathogens associated with human gastroenteritis worldwide. In Europe, campylobacteriosis is one of the leading food-borne bacterial diseases and the consumption of poultry meats is suspected to be one of the major causes of illness. The aim of our research was to determine the number of Campylobacter spp. in poultry carcasses and in poultry meat samples during their storage till to retail markets. The study was conducted from February 2009 to February 2010 at slaughterhouse in Veneto region, followed by a test of fresh poultry meat placed on the market for sale. A total of 90 poultry carcass and 90 samples of poultry meat were examined. The quantitative examination resulted in Campylobacter spp. counts (mean): for carcasses between 2,0 101 ufc/g and 1,5 103 ufc/g (4,2 102) and poultry meat between 2,0 101 ufc/g and 3,7 102 ufc/g (8,1 101). The majority of isolates were classified as Campylobacter jejuni (58,3%), Campylobacter coli (22,9%) or Arcobacter cryaerophilus (4,2%). Acknowledgments: The project was funded with grants from Fondazione Cariverona 2007.
A NEW DISCOLORATION OF RICOTTA CHEESE
L. Alberghini,G Tallone,V. Giaccone
Italian Journal of Food Safety , 2010, DOI: 10.4081/ijfs.2010.8.7
Abstract: A new alteration of ricotta cheese is here described. The discoloration which has been noted was red. The responsible bacteria has been identified as Serratia marcescens. This is probably the first report of this rare type of spoilage identified in Italy.
MICROBIOLOGICAL EVALUATION OF WATER BUFFALO COLOSTRUM: FIRST RESULTS
P. Catellani,L. Alberghini,V. Giaccone
Italian Journal of Food Safety , 2012, DOI: 10.4081/ijfs.2012.3.77
Abstract: Animal colostrum contains many substances with antibacterial activity such as lysozyme and lactoferrin which should inhibit the microbial growth. The aim of this research is to understand if colostrum can be considered a safe product, considering that Regulation (EC) N° 1662/2006, which has modified the Regulation (EC) N° 853/2004, introducing colostrum as human food. Microbiological tests, made on water buffalo colostrum, aiming to obtain the total microbial count (maximum concentration: 3,6 104 ufc/ml), the quantitative evaluation of total (maximum the highest concentration found: 2,3 103 ufc/ml) and fecal coliforms (maximum concentration: 4,9 102 ufc/ml) and the qualitative search of Salmonella spp. and Listeria monocytogenes, showed that there is not a microbial growth in colostrum, that it can be considered a safe food, from the microbial point of view
MICROBIAL STATUS OF DONKEY’S MILK: FIRST RESULTS
L. Alberghini,P. Catellani,M.A. Norbiato,V. Giaccone
Italian Journal of Food Safety , 2012, DOI: 10.4081/ijfs.2012.3.7
Abstract: For its chemical and nutritional composition donkey milk is an excellent substitute for human breast milk. In fact, woman’s milk and donkey one are quite similar for their composition and for certain intrinsic properties. Based on these considerations, we performed a study on the microbiological characteristics of 86 jannet’s milk in 10 farms. Special attention was given to the aspects of health quality of the milk samples examined, referring to the analysis of total microbial count and pH, the possible presence of potentially pathogenic micro-organisms, especially by seeking the bacteria Escherichia coli O157, Salmonella spp., Listeria monocytogenes and Campylobacter spp. The samples of milk were picked up from January to March 2010. Our research showed a situation quite positive in terms of sanitation, finding levels of total microbial count (on average 1,8·104 cfu/ml) and pH (mean 7,02) entirely in line with data found in previous investigations of other Authors and with values reassuring in terms of healthiness of the product. Regarding the detection of potentially pathogenic microorganisms, only a milk sample of 86 tested was positive for E. coli O157; in an other sample low charges of Campylobacter spp. have been measured. Salmonella spp. and Listeria monocytogenes were not found in all samples. Total microbial count confirm the good quality of the donkey milk. However, occasional isolation of E. coli O157 and Campylobacter spp. suggests careful attention is to be done to the correct procedures of milking hygiene.
Fibrosarcomatous changes and expression of CD34+ and apolipoprotein-D in dermatofibrosarcoma protuberans
Emanuela Palmerini, Marco Gambarotti, Eric L Staals, Licciana Zanella, Gabriela Sieberova, Alessandra Longhi, Marilena Cesari, Stefano Bonarelli, Piero Picci, Pietro Ruggieri, Marco Alberghini, Stefano Ferrari
Clinical Sarcoma Research , 2012, DOI: 10.1186/2045-3329-2-4
Abstract: DFSP and fibrosarcomatous-DFSP (FS-DFSP) patients referred to our institute between 1982 and 2009 were identified. Fibrosarcomatous changes, expression of CD34 and apolipoprotein-D were evaluated.40 patients, (median age 43 years, 55% males) were identified. Tumor was located in the limbs in 60%, in the trunk in 40%. Thirty-seven patients had localized and 3 had metastatic disease. Thirteen (32%) patients were FS-DFSP. All but one underwent surgery with adequate surgical margins in 72%. 7 FS-DFSP received also radiotherapy (RT). Chemotherapy was administered to 3 patients with FS-DFSP. With a median follow-up of 49 months, the 5-OS was 90%. Local recurrence rate was 23%: 42% FS-DFSP, 15% DFSP. Metastases developed in three FS-DFSP patients. The 5-year EFS was 70% in localized patients. Histology (DFSP 75% vs. FS-DFSP 52%, p = 0.002), surgical margins (adequate 74% vs. inadequate 55%, p = 0.02), site (limb 47% vs. trunk 100%), CD34 expression (CD34 positive: 70% vs. CD34 negative: 33%, p = 0.05), and apolipoprotein-D expression (Apo-D positive: 73% vs. Apo-D negative: 33%, p = 0.02) influenced the 5-year EFS, whereas sex, use of RT or number of previous surgical treatments did not.Patients with DFSP have a high survival probability. Site, adequate surgical margins, presence of the fibrosarcomatous component, lack of CD34 expression and apolipoprotein-D influence outcome.Dermatofibrosarcoma protuberans (DFSP) is a low grade malignant mesenchymal tumor that typically arises in the dermis of the trunk and proximal extremities [1]. DFSP represents 1 to 6% of all soft tissue sarcomas (STS) [2,3] and its frequency of detection slowly has increased over time [4]. DFSP is characterized by latency of initial diagnosis, slow infiltrative growth and a high rate of local recurrence if not adequately treated. Death due to metastatic disease is very rare (< 5%) [5]. Histologically, DFSP is usually characterized by uniform spindle shaped cells with elongated neuroid nuclei, prolife
Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti
Roberto Moscatiello, Sara Alberghini, Andrea Squartini, Paola Mariani, Lorella Navazio
BMC Microbiology , 2009, DOI: 10.1186/1471-2180-9-206
Abstract: A plasmid encoding the bioluminescent Ca2+ probe aequorin was introduced into Mesorhizobium loti USDA 3147T strain to investigate whether a Ca2+ response is activated in rhizobia upon perception of plant root exudates. We find that M. loti cells respond to environmental and symbiotic cues through transient elevations in intracellular free Ca2+ concentration. Only root exudates from the homologous host Lotus japonicus induce Ca2+ signalling and downstream activation of nodulation genes. The extracellular Ca2+ chelator EGTA inhibits both transient intracellular Ca2+ increase and inducible nod gene expression, while not affecting the expression of other genes, either constitutively expressed or inducible.These findings indicate a newly described early event in the molecular dialogue between plants and rhizobia and highlight the use of aequorin-expressing bacterial strains as a promising novel approach for research in legume symbiosis.Rhizobia are Gram-negative soil bacteria which can engage in a mutualistic association with leguminous plants. Under nitrogen-limiting conditions, rhizobia colonize plant roots and highly specialized plant organs, the nodules, are generated de novo on host roots (for a recent review see [1]). When living symbiotically, rhizobia are able to fix atmospheric nitrogen into forms usable by the plant. In return, they receive dicarboxylic acids as a carbon and energy source for their metabolism. Nitrogen is the most frequent limiting macronutrient in many soils, and it is generally supplied as fertilizer. The rhizobium-legume mutualistic association can reduce or eliminate nitrogen fertilizer requirements, resulting also in a benefit to the environment [2].A successful symbiosis is the result of an elaborate developmental program, regulated by the exchange of molecular signals between the two partners [3]. During growth in the rhizosphere of the host plant, rhizobia sense compounds secreted by the host root and respond by inducing bacterial nodul
Occurrence of enterotoxin genes and macrorestriction analysis of Staphylococcus aureus isolated from bovine mastitis and bulk-tank milk samples in Italy. An epidemiological study
Andrea Serraino,Leonardo Alberghini,Maria Cristina Fontana,Cosima Annemu?ller
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2004.47
Abstract: The goal of the study was to genotypically compare S. aureus isolates from mastitis milk and raw milk to identify the relation between strains and to assess the enterotoxigenicity of the isolates. Eighty-three Staphylococcus aureus isolates recovered from cows and bulk tank milk of five farms in northern Italy were compared genotypically. The genes for the enterotoxins A, D, G and I, but not for B, C, E and H and the toxic shock syndrome toxin 1 (TSST-1), were detected by PCR amplification. Macrorestriction analysis with the restrictions enzyme SmaI revealed 14 pulsed-field gel electrophoresis patterns. These were in part different from each other only in a few fragments and thus displayed a close clonal relation. The results of the present investigation showed that identical or closely related clones seemed to be responsible for the cases of bovine mastitis in the farms investigated and partly responsible for contamination of bulk tank milk.
Histochemical and ultrastructural study of an elastofibroma dorsi coexisting with a high grade spindle cell sarcoma
M Alberghini,P Bacchini,G Pignatti,MC Maltarello
European Journal of Histochemistry , 2004, DOI: 10.4081/884
Abstract: Elastofibroma dorsi is a pseudotumoral fibroproliferative lesion characterized by polymorphic fiber-like deposits of elastinophilic material. Several theories have been reported explaining the pathogenesis of elastofibroma. Recent cytogenetic studies have demonstrated chromosomal instability in elastofibromas, not normally observed in non-neoplastic tissues. These chromosomal defects are commonly observed in aggressive fibromatosis too. Such clinical observations suggest a multistage pathogenetic mechanism for the onset of elastofibroma. This study, using histochemical, immunohistochemical staining techniques, and ultrastructural examination, describes the detection of an otherwise typical elastofibroma contextual to a high grade sarcoma. Hence, the coexistence of elastofibroma and high-grade sarcoma may suggest a causal link between the two pathological entities. The results obtained suggest that the coexistence of the two pathological entities is conceivably coincidental.
Parosteal osteosarcoma mimicking osteochondroma: A radio-histologic approach on two cases
Zafiria G Papathanassiou, Marco Alberghini, Philippe Thiesse, Marco Gambarotti, Giuseppe Bianchi, Cristina Tranfaglia, Daniel Vanel
Clinical Sarcoma Research , 2011, DOI: 10.1186/2045-3329-1-2
Abstract: The review of 86 parosteal osteosarcomas of bone revealed this atypical pattern only once. A consultation case was received in the same time, and added to ours. Patients were 28 years old and 56 years old females. Imaging studies included two radiographs, two CTscans, one MRI examination and one bone scan and the results were compared to histology.On imaging, both lesions presented as ossified lobulated masses attached with a broad base to the underlying cortex. No radiolucent cleft separated the masses and the host bone and cortex continuity between the mass and the femur was seen, with medullary communication. The marrow of the mass had a different density and intensity compared to normal marrow. So, there were features of an osteochondroma (cortex and medullary continuity) and of a parosteal osteosarcoma (ossified marrow). Pathological assessment on the final specimen confirmed the presence of low-grade parosteal osteosarcomas, after an erroneous diagnosis of osteochondroma on the initial biopsy.Parosteal osteosarcoma can be rarely confused with osteochondroma. A radiologic-pathologic correlation is essential. Cortex continuity is the most misleading imaging feature that may occur in parosteal osteosarcomas. A knowledge of this misleading pattern will help diagnose the lesion from the beginning.Parosteal osteosarcoma (POS) is a slow-growing tumor which originates from the outer layer of the periosteum and represents 65% of surface osteosarcomas [1] and in our database accounts approximately for 4, 8% of all osteosarcomas [2]. Unlike conventional osteosarcomas, it involves an older age group typically in the 3rd and 4th decades of life and shows a slight female predilection [1,3,4]. The most common location of a POS is the posterior aspect of the distal femur accounting for approximately two thirds of all cases [5]. Confusion may rarely occur in differentiating POS from the sessile variant of osteochondroma on imaging studies. Herein, we report two cases of POS wi
Imaging of hibernomas: A retrospective study on twelve cases
Zafiria G Papathanassiou, Marco Alberghini, Sophie Taieb, Costantino Errani, Piero Picci, Daniel Vanel
Clinical Sarcoma Research , 2011, DOI: 10.1186/2045-3329-1-3
Abstract: Twelve hibernomas were retrospectively assessed with CT and MR imaging and compared to the histology of the specimenNine females and three males with a mean age of 30 years were included. Ten tumors occurred in the thigh and two affected the subcutis of the periscapular and buttock regions. On eight CT scans, seven (87,5%) lesions were homogeneous and mildly to moderately hyperdense compared to subcutaneous fat while one lesion was heterogeneous with mixed hypo and hyperattenuating areas. On six T1W images, five (83,3%) lesions appeared homogeneous and hypointense relative to subcutaneous fat and one was heterogeneous. Incomplete fat suppression was depicted in all cases. All lesions displayed marked enhancement. Large intratumoral vessels were depicted in three of the 12 (25%) cases. Septations were depicted on four of the eight unenhanced CT and on all six MRI examinations.Hibernoma usually appears hypodense and hypointense relative to subcutaneous fat on pre-contrast CT and MR T1W with variable enhancement patterns and incomplete fat suppression on STIR or fat-saturated sequences. These characteristics relate directly to the presence of brown fat. However, atypical findings such as heterogeneous patterns of mixed fatty and non fatty components on unenhanced CT and MR T1W can be also encountered. Absence of large intratumoral vessels should not exclude hibernomas from the differential diagnosis of regional lipomatous tumors.Hibernomas are rare benign lipomatous tumors originating from residual brown fat cells. At the beginning of the century, Merkel [1] first described them as"pseudolipomas". Owing to their resemblance to the brown fat of hibernating animals, the term "hibernoma" was coined by Gery in 1914 [2]. They affect chiefly adults in the 3rd of 4th decades of life [3] and they usually grow in the vestiges, where brown fat is found in fetuses and infants, such as the shoulder, neck, axilla, the periscapular and interscapular area, thorax and retroperitoneum
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