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Search Results: 1 - 10 of 592435 matches for " L. A. Conboy "
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Moving Beyond Health to Flourishing: The Effects of Yoga Teacher Training
L. A. Conboy,A. Wilson,T. Braun
The Scientific World Journal , 2010, DOI: 10.1100/tsw.2010.87
Abstract:
Ayurveda and Panchakarma: Measuring the Effects of a Holistic Health Intervention
L. A. Conboy,Ingrid Edshteyn,Hilary Garivaltis
The Scientific World Journal , 2009, DOI: 10.1100/tsw.2009.35
Abstract:
Simple and Efficient Methods for Enrichment and Isolation of Endonuclease Modified Cells
Branden S. Moriarity, Eric P. Rahrmann, Dominic A. Beckmann, Caitlin B. Conboy, Adrienne L. Watson, Daniel F. Carlson, Erik R. Olson, Kendra A. Hyland, Scott C. Fahrenkrug, R. Scott McIvor, David A. Largaespada
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096114
Abstract: The advent of Transcription Activator-Like Effector Nucleases (TALENs), and similar technologies such as CRISPR, provide a straightforward and cost effective option for targeted gene knockout (KO). Yet, there is still a need for methods that allow for enrichment and isolation of modified cells for genetic studies and therapeutics based on gene modified human cells. We have developed and validated two methods for simple enrichment and isolation of single or multiplex gene KO's in transformed, immortalized, and human progenitor cells. These methods rely on selection of a phenotypic change such as resistance to a particular drug or ability to grow in a selective environment. The first method, termed co-transposition, utilizes integration of a piggyBac transposon vector encoding a drug resistance gene. The second method, termed co-targeting, utilizes TALENs to KO any gene that when lost induces a selectable phenotype. Using these methods we also show removal of entire genes and demonstrate that TALENs function in human CD34+ progenitor cells. Further, co-transposition can be used to generate conditional KO cell lines utilizing an inducible cDNA rescue transposon vector. These methods allow for robust enrichment and isolation of KO cells in a rapid and efficient manner.
Cell Cycle Genes Are the Evolutionarily Conserved Targets of the E2F4 Transcription Factor
Caitlin M. Conboy, Christiana Spyrou, Natalie P. Thorne, Elizabeth J. Wade, Nuno L. Barbosa-Morais, Michael D. Wilson, Arindam Bhattacharjee, Richard A. Young, Simon Tavaré, Jacqueline A. Lees, Duncan T. Odom
PLOS ONE , 2007, DOI: 10.1371/journal.pone.0001061
Abstract: Maintaining quiescent cells in G0 phase is achieved in part through the multiprotein subunit complex known as DREAM, and in human cell lines the transcription factor E2F4 directs this complex to its cell cycle targets. We found that E2F4 binds a highly overlapping set of human genes among three diverse primary tissues and an asynchronous cell line, which suggests that tissue-specific binding partners and chromatin structure have minimal influence on E2F4 targeting. To investigate the conservation of these transcription factor binding events, we identified the mouse genes bound by E2f4 in seven primary mouse tissues and a cell line. E2f4 bound a set of mouse genes that was common among mouse tissues, but largely distinct from the genes bound in human. The evolutionarily conserved set of E2F4 bound genes is highly enriched for functionally relevant regulatory interactions important for maintaining cellular quiescence. In contrast, we found minimal mRNA expression perturbations in this core set of E2f4 bound genes in the liver, kidney, and testes of E2f4 null mice. Thus, the regulatory mechanisms maintaining quiescence are robust even to complete loss of conserved transcription factor binding events.
High Incidence of Non-Random Template Strand Segregation and Asymmetric Fate Determination In Dividing Stem Cells and their Progeny
Michael J. Conboy,Ariela O. Karasov,Thomas A. Rando
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0050102
Abstract: Decades ago, the “immortal strand hypothesis” was proposed as a means by which stem cells might limit acquiring mutations that could give rise to cancer, while continuing to proliferate for the life of an organism. Originally based on observations in embryonic cells, and later studied in terms of stem cell self-renewal, this hypothesis has remained largely unaccepted because of few additional reports, the rarity of the cells displaying template strand segregation, and alternative interpretations of experiments involving single labels or different types of labels to follow template strands. Using sequential pulses of halogenated thymidine analogs (bromodeoxyuridine [BrdU], chlorodeoxyuridine [CldU], and iododeoxyuridine [IdU]), and analyzing stem cell progeny during induced regeneration in vivo, we observed extraordinarily high frequencies of segregation of older and younger template strands during a period of proliferative expansion of muscle stem cells. Furthermore, template strand co-segregation was strongly associated with asymmetric cell divisions yielding daughters with divergent fates. Daughter cells inheriting the older templates retained the more immature phenotype, whereas daughters inheriting the newer templates acquired a more differentiated phenotype. These data provide compelling evidence of template strand co-segregation based on template age and associated with cell fate determination, suggest that template strand age is monitored during stem cell lineage progression, and raise important caveats for the interpretation of label-retaining cells.
High Incidence of Non-Random Template Strand Segregation and Asymmetric Fate Determination In Dividing Stem Cells and their Progeny
Michael J Conboy,Ariela O Karasov,Thomas A Rando
PLOS Biology , 2007, DOI: 10.1371/journal.pbio.0050102
Abstract: Decades ago, the “immortal strand hypothesis” was proposed as a means by which stem cells might limit acquiring mutations that could give rise to cancer, while continuing to proliferate for the life of an organism. Originally based on observations in embryonic cells, and later studied in terms of stem cell self-renewal, this hypothesis has remained largely unaccepted because of few additional reports, the rarity of the cells displaying template strand segregation, and alternative interpretations of experiments involving single labels or different types of labels to follow template strands. Using sequential pulses of halogenated thymidine analogs (bromodeoxyuridine [BrdU], chlorodeoxyuridine [CldU], and iododeoxyuridine [IdU]), and analyzing stem cell progeny during induced regeneration in vivo, we observed extraordinarily high frequencies of segregation of older and younger template strands during a period of proliferative expansion of muscle stem cells. Furthermore, template strand co-segregation was strongly associated with asymmetric cell divisions yielding daughters with divergent fates. Daughter cells inheriting the older templates retained the more immature phenotype, whereas daughters inheriting the newer templates acquired a more differentiated phenotype. These data provide compelling evidence of template strand co-segregation based on template age and associated with cell fate determination, suggest that template strand age is monitored during stem cell lineage progression, and raise important caveats for the interpretation of label-retaining cells.
Algumas medidas típicas univariadas da magnitude do efeito
Conboy,Joseph Edward;
Análise Psicológica , 2003,
Abstract: measures of effect size represent an alternative to traditional methods of data analysis in the social sciences. these metrics avoid some of the most evident problems associated with tests of hypotheses, are more simply interpreted than sometimes misleading p values, and provide the basis for the synthesis of large numbers of research studies. this text assumes that the reader has a basic understanding of inferential statistics and describes the calculation and interpretation of common forms of effect size that can be used either as alternatives, or complements, to classical hypothesis testing.
An Action Research Case Study of the Facilitators and Inhibitors of E-Commerce Adoption
Orla Kirwan,Kieran Conboy
International Business Research , 2009, DOI: 10.5539/ibr.v2n2p48
Abstract: This research has studied an established Irish retail business as it takes its first tentative steps into the e-commerce arena. Although the adoption of e-commerce is widely studied in the academic world, only a small percentage of these studies focus on the Small to Medium size Enterprise (SME) retail sector. SMEs account for 97% of Irish companies and employ up to 800,000 people (Chamber of Commerce Ireland, 2006). Whilst examining the SME’s adoption of e-commerce, the factors that affected the adoption process were specifically identified and understood. This was achieved by conducting an action research case study. Action research merges research and practice thus producing exceedingly relevant research findings. The case study commenced in August 2003 and concluded in August 2004. It consisted of three distinctive action research cycles. The researcher worked in the SME throughout the research process, and had been employed there for the previous 5 years. This chapter demonstrates how the research was undertaken, and also discusses the justification, benefits and limitations of using action research. The research concluded that the adoption of e-commerce within the SME sector tends to be slow and fragmented, the presence of a “web champion” is paramount to the success of the project and Internet adoption is faster with the recognition of a business need. It also supported the evidence that an SME is more likely to adopt e-commerce when the SME owner has a positive attitude to IT.
Aten o e memória visual na popula o idosa: uma associa o etre as habilidades literárias sob condi es de interferência.
Pedro Gabriel,Joseph Conboy
Cuadernos de Neuropsicologia , 2010,
Abstract: Deterioration in attention and memory is frequently observed in the elderly. Other factors, besides age, contribute to this deterioration, of course. In this study we describe an association between levels of formal education and deficits in attention and visual memory in the presence of interference. The participants (57 individuals between the ages of 65 and 92) responded to the Toulose-Piéron test and the Visual Reproduction sub-test of the Wechsler-R. The Stroop test was used to create interference conditions in the visual memory tasks. Independent of age, those seniors with more formal education performed better in the tests of concentration and on visual memory tasks in the presence of interference.
Smooth transitions from Schwarzschild vacuum to de Sitter space
Steven Conboy,Kayll Lake
Physics , 2005, DOI: 10.1103/PhysRevD.71.124017
Abstract: We provide an infinity of spacetimes which contain part of both the Schwarzschild vacuum and de Sitter space. The transition, which occurs below the Schwarzschild event horizon, involves only boundary surfaces (no surface layers). An explicit example is given in which the weak and strong energy conditions are satisfied everywhere (except in the de Sitter section) and the dominant energy condition is violated only in the vicinity of the boundary to the Schwarzschild section. The singularity is avoided by way of a change in topology in accord with a theorem due to Borde..
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