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Search Results: 1 - 10 of 213623 matches for " Lázara Rojas "
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Estudio de la susceptibilidad al metronidazol en aislamientos cubanos de Trichomonas vaginalis
Sariego Ramos,Idalia; Rojas Rivero,Lázara; Fraga Nodarse,Jorge;
Revista Cubana de Obstetricia y Ginecolog?-a , 2011,
Abstract: the trichomoniasis is a very frequent infection at world level with significant medical and social implications. the metronidazole is the choice drug to treat this infection. however, it was demonstrated the existence of parasite strains resistant to this drug in some countries. in cuba, most of treatment failures seem to be due to the non-fulfillment of prescribed therapy or to reinfections. nevertheless, some of them remain without an apparent explanation and could be circulating resistant isolates. the objective of the present paper is to determine the susceptibility to metronidazole of the cuban isolates of trichomonas vaginalis. forty isolates of this parasite were studied, from the vaginal exudates of adolescents attended at the gynecobstetrics hospitals of havana. the isolates underwent in vitro trials under anaerobic and aerobic conditions, to know their susceptibility or resistance to metronidazole. serial double dilutions of metronidazole between 400 μg/ml and 0.2 μg/ml and the dimethylsulfoxide as solvent were used. according to the trials carried out under aerobic conditions, two resistant isolates to metronidazole (tv-352, clm: 50 μg/ml and tv-240, clm: 200 μg/ml) were detected. this finding alerts us about the need to deepen in causes of treatment failures to make an appropriate assessment in order to apply the suitable treatment.
Estudio de la susceptibilidad al metronidazol en aislamientos cubanos de Trichomonas vaginalis Study on metronidazole susceptibility in Cuban isolates of Trichomonas vaginalis
Idalia Sariego Ramos,Lázara Rojas Rivero,Jorge Fraga Nodarse
Revista Cubana de Obstetricia y Ginecolog?-a , 2011,
Abstract: La trichomonosis es una infección muy frecuente a nivel mundial con importantes implicaciones médicas y sociales. El metronidazol constituye el fármaco de elección para el tratamiento de esta infección. Sin embargo, se ha demostrado la existencia de cepas del parásito resistentes a este medicamento en varios países. En el nuestro, la mayoría de los fallos de tratamiento parecen deberse al incumplimiento con la terapia indicada o a reinfecciones. No obstante, algunos quedan sin explicación aparente y pudieran estar circulando aislamientos resistentes. El objetivo es determinar la susceptibilidad al metronidazol de aislamientos cubanos de Trichomonas vaginalis. Se estudiaron 40 aislamientos del parásito, procedentes de exudados vaginales de adolescentes, atendidas en hospitales ginecoobstétricos de Ciudad de La Habana. Los aislamientos fueron sometidos a ensayos in vitro, tanto en condiciones anaeróbicas como aeróbicas, para conocer su susceptibilidad o resistencia al metronidazol. Se utilizaron diluciones dobles seriadas de metronidazol entre 400 μg/mL y 0,2 μg/mL y el dimetilsulfóxido como solvente. Mediante los ensayos realizados en condiciones aeróbicas se detectaron dos aislamientos resistentes al metronidazol (Tv-352, CLM: 50 μg/mL y Tv-240, CLM: 200 μg/mL). Este hallazgo nos alerta acerca de la necesidad de profundizar en las causas de los fallos de tratamiento para hacer una correcta evaluación e imponer el tratamiento adecuado. The trichomoniasis is a very frequent infection at world level with significant medical and social implications. The metronidazole is the choice drug to treat this infection. However, it was demonstrated the existence of parasite strains resistant to this drug in some countries. In Cuba, most of treatment failures seem to be due to the non-fulfillment of prescribed therapy or to reinfections. Nevertheless, some of them remain without an apparent explanation and could be circulating resistant isolates. The objective of the present paper is to determine the susceptibility to metronidazole of the Cuban isolates of Trichomonas vaginalis. Forty isolates of this parasite were studied, from the vaginal exudates of adolescents attended at the gynecobstetrics hospitals of Havana. The isolates underwent in vitro trials under anaerobic and aerobic conditions, to know their susceptibility or resistance to metronidazole. Serial double dilutions of metronidazole between 400 μg/mL and 0.2 μg/mL and the dimethylsulfoxide as solvent were used. According to the trials carried out under aerobic conditions, two resistant isolates to metronida
Aplicación de un método alternativo al conteo en cámara de Neubauer para determinar la concentración de Trichomonas vaginalis
Meneses Marcel,Alfredo; Rojas,Lázara; Sifontes Rodríguez,Sergio; López,Yamilé; Sariego Ramos,Idalia;
Revista Cubana de Medicina Tropical , 2001,
Abstract: it was demonstrated that there is a relation of cell concentration with the parasite reading in an elisa reader. it was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. likewise, it was confirmed that absorbance significantly increased (p<0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of the various cell concentrations, it was proved that the optimum volume was 150 ml, a r2= 0,9986 was reached, and the coefficient of correction(p<0,001) and the gradient(p<0,001)were highly significant. in the 5 x 104 to 1.5 x 107 cell/ml interval, there was an average variation coefficient of 1.75% (0.25-3.17%). under these conditions, the quantification limit was 5,14 x 104 cell/ml. finally, it was shown that the correlation of counting in neubauer chamber and optical density was significant.
Aplicación de un método alternativo al conteo en cámara de Neubauer para determinar la concentración de Trichomonas vaginalis
Alfredo Meneses Marcel,Lázara Rojas,Sergio Sifontes Rodríguez,Yamilé López
Revista Cubana de Medicina Tropical , 2001,
Abstract: Se demostró que existe relación entre la concentración celular y la lectura de los parásitos en un lector de ELISA. Se determinó que la absorbancia tiene valores significativos en las longitudes de onda del rango visible y se escogió la longitud de onda mínima posible (450 nm) para garantizar un máximo de sensibilidad. Pudo comprobarse que ocurre un aumento significativo de la absorbancia (p<0,001) después de llenada la placa, que se estabiliza en el intervalo de 40 min hasta 4 h. Al aplicar 100, 150, 200 ó 300 μL por pozo de las diferentes concentraciones de células se demostró que el volumen óptimo era de 150 μL, se obtuvo un r2 = 0,9986, y resultaron altamente significativos el coeficiente de correlación (p<0,001) y la pendiente (p<0,001). En el intervalo de 5 x 104 a 1,5 x 107 células/mL se obtuvo un coeficiente de variación medio de 1,75 % (0,25-3,17 %). En estas condiciones el límite de cuantificación fue de 5,14 x 104 células/mL. Por último se demostró que hubo significación de la correlación entre el conteo en cámara de Neubauer y la densidad óptica. It was demonstrated that there is a relation of cell concentration with the parasite reading in an ELISA reader. It was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. Likewise, it was confirmed that absorbance significantly increased (p<0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of the various cell concentrations, it was proved that the optimum volume was 150 ml, a r2= 0,9986 was reached, and the coefficient of correction(p<0,001) and the gradient(p<0,001)were highly significant. In the 5 x 104 to 1.5 x 107 cell/mL interval, there was an average variation coefficient of 1.75% (0.25-3.17%). Under these conditions, the quantification limit was 5,14 x 104 cell/mL. Finally, it was shown that the correlation of counting in Neubauer chamber and optical density was significant.
Toxocariasis in Cuba: A Literature Review
Idalia Sariego ,Kirezi Kanobana,Lázara Rojas,Niko Speybroeck,Katja Polman,Fidel A. Nú?ez
PLOS Neglected Tropical Diseases , 2012, DOI: 10.1371/journal.pntd.0001382
Abstract: Human toxocariasis (HT) is a zoonotic disease caused by infection with the larval stage of Toxocara canis, the intestinal roundworm of dogs. Infection can be associated with a wide clinical spectrum varying from asymptomatic to severe organ injury. While the incidence of symptomatic human toxocariasis appears to be low, infection of the human population is widespread. In Cuba, a clear overview on the status of the disease is lacking. Here, we review the available information on toxocariasis in Cuba as a first step to estimate the importance of the disease in the country. Findings are discussed and put in a broader perspective. Data gaps are identified and suggestions on how to address these are presented. The available country data suggest that Toxocara infection of the definitive dog host and environmental contamination with Toxocara spp. eggs is substantial, but information on HT is less conclusive. The availability of adequate diagnostic tools in the country should be guaranteed. Dedicated studies are needed for a reliable assessment of the impact of toxocariasis in Cuba and the design of prevention or control strategies.
Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
Fraga, Jorge;Rojas, Lázara;Sariego, Idalia;Fernández-Calienes, Aymé;
Brazilian Journal of Infectious Diseases , 2005, DOI: 10.1590/S1413-86702005000600012
Abstract: trichomonas vaginalis can be infected with double-stranded rna (dsrna) viruses designated t. vaginalis virus (tvv), which may have important implications for trichomonal virulence and disease pathogenesis. we tested for tvv in 40 fresh t. vaginalis isolates from cuban patients by total extraction of nucleic acids (dna and rna). tvv was detected in 22 (55%) of the 40 t. vaginalis isolates. this gives an estimate of the infection rate of cuban t. vaginalis isolates by the dsrna virus. future research should focus on the association between trichomonosis symptoms and the presence of tvv.
Comportamiento "in vivo" de aislamientos de Trichomonas vaginalis, utilizando ratones NMRI como modelo experimental
Rojas R.,Lázara; Sariego R.,Idalia; Sarría P.,Carlos; Fraga N.,Jorge;
Revista chilena de infectología , 2004, DOI: 10.4067/S0716-10182004000300001
Abstract: the correlation between clinical severity of vaginal trichomoniasis in adolescents and trichomonas vaginalis virulence in an experimental model was studied. for this purpose, four hundred mice were inoculated intraperitoneally with 40 trichomonas vaginalis isolates obtained from vaginal exudates. these were obtained from adolescents clinically classified as asymptomatic or mild, moderate or severe symptomatic patients, attending the infant-juvenile service of pregnancy interruption and sexually-transmitted diseases from gynecoobstetrics hospitals of the city of havana. the ideal parasite concentration to inoculate turned out to be 8 x 106. the liver was the most affected organ, with a significant difference (p < 0,01) from the rest of the organs. through kruskal-wallis test it was possible to observe that there were significant differences (p < 0,0001) among the score obtained by each mice group, depending on the kind of isolation, according to the clinical classification of origin. this demonstrated a possible correlation between the degree of virulence of each isolate and the clinical characteristics of the disease-carrying patients
Avances más recientes en el desarrollo de vacunas contra la malaria
Rivero,Lázara Rojas; Pérez,Jorge Sarracent; Galindo,Luis Fonte;
Revista Panamericana de Salud Pública , 2005, DOI: 10.1590/S1020-49892005000200013
Abstract: according to reports of the pan american health organization, malaria transmission continues to occur in 21 countries of the americas. of the 835 million inhabitants of the region of the americas, 293 million live in areas with some possibility of transmission of the disease. the most advanced of the candidate vaccines that have been designed based on the sequences of the circumsporozoite protein, is one based on the rts,s/as02a polypeptides of plasmodium falciparum. a test of that vaccine was conducted in mozambique with children from 1 to 4 years old. the test proved the vaccine to be safe, well tolerated, and immunogenic, but the level of protection reached was still low. however, the advantages that the rts,s/as02a vaccine offers to people who live in malaria-endemic areas justifies its being tested in the americas in order to evaluate its effectiveness in the clinical and epidemiological conditions specific to the region.
ENZYMEBA, procedimiento eficaz para estudiar la prevalencia de infección intestinal por Entamoeba histolytica
Nú?ez Fernández,Fidel; Montalvo,Ana Margarita; Rojas Rivero,Lázara; Alberti Amador,Esteban;
Revista Cubana de Higiene y Epidemiolog?-a , 1998,
Abstract: owing to the epidemiological usefulness of the development of methods allowing reliable studies on the prevalence of intestinal amebiasis, this paper studied the effectiveness of enzymeba, inmuno-enzymatic assay to detect entamoeba histolytica from feces. through only one feces specimen from every participant in the study, this assay detected e. histolytica infection in 27 out of the 686 cases observed. the results of microscopic feces observation were only comparable to those of enzymeba when the result of both methods were carefully examined in 3 feces specimen taken from each of the subjects participating in it. because this inmunoassay is highly sensitive, requires only one feces specimen per surveyed person and can be perfomed at a minimal laboratory conditions, enzymeba is considered to be a very useful tool for studying the prevalence of intestinal amebiasis.
Frecuencia de trichomoniasis vaginal en mujeres suspuestamente sanas
Rojas Rivero,Lázara; Solano Silveira,Rubén; Sariego Ramos,Idalia;
Revista Cubana de Higiene y Epidemiolog?-a , 1999,
Abstract: the frequency of trichomoniasis in 5 hospitals from different municipalities of havana city was studied among 427 women with no history of gynecological diseases that attended the microbiology laboratories of these centers from may to september, 1997. 35 women of the total of women studied proved to be carriers of trichomonas vaginalis, which accounts for a positivity frequency of 8.2 %. according to age, the highest percentaje of positivity (16.1) corresponded to the group aged 15-20. the diagnosis by culture in vitro had a greater sensitivity compared with the direct examination of the vaginal exudate. the reading obtained at 48 hours showed the highest diagnostic positivity.
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