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The Effectiveness of Treatment Training for Children with Acute Respiratory Tract Infection to Mothers of Toddlers in Bandung Indonesian  [PDF]
Henny Cahyaningsih, Sri Kusmiati, Ahmad Husni
Open Journal of Nursing (OJN) , 2017, DOI: 10.4236/ojn.2017.77057
Abstract: Acute Respiratory Tract Infection (ARTI) remains a health problem for toddlers in Indonesia and the leading cause of death for toddlers. In addition to causing health problems, ARTI can also cause death. Indonesia, as one of the countries signing of the Millennium Development Goals (MDGs), is required to be able to reduce toddler mortality rate. The research intends to find the effectiveness of treatment training for children with ARTI in increasing the knowledge, attitude, and skill of mothers of toddlers in the working area of Community Health Centre Sukajadi, Bandung. It employed a quasiexperimental method with the pre-posttest two group designs. The sample, taken with the random sampling technique, consisted of 26 mothers of toddlers for the control and intervention groups, respectively. For the intervention group, the treatment training for children with ARTI was given for 4 - 5 hours in one day, and the control group was only given a conventional extension program for 15 minutes. The knowledge, attitudes, and skills before and after intervention were measured three days after the training using the same instrument used during the pretest. The results of this research show that in terms of the effectiveness of the training using modules, there were differences in the average scores for knowledge with p value (0.030 ≤ α (0.05), for the aspect of attitudes with p value (0.046) ≤ α (0.05), and for the sub-variable of skills as proven by the p value of (0.046) ≤ α (0.05). Based on these results, the intervention of ARTI treatment training at home had significant effect on the knowledge, attitudes, and skills of the toddlers’ mothers. With this inference, it is recommended that the research results can be made a topic of study for the development of a training model or standardized training guidelines that will be used by health officers at Community Health Centre Sukajadi, Bandung City Indonesian.
Salsa Dance Could Improve Effectively to Body Balance in Elderly with Risk of Falls  [PDF]
Sri Kusmiati, Endeh Nurgiwiati, Ali Hamzah
Open Journal of Nursing (OJN) , 2018, DOI: 10.4236/ojn.2018.811058
Abstract: Currently the number of elderly in Indonesia tends to increase. Based on 2014 Susenas data, the number of elderly in Indonesia reaches 20.24 million people (8.03%). One of the most common physical disorders in the elderly is disruption of the body’s balance and risk to falls. The risk of falling in the elderly can be prevented by doing physical exercise in the form of Salsa dance. Salsa dance has movements that can improve stability, flexibility, mobility and body balance. This study aims to analyze the effectiveness of Salsa dance on body balance in the elderly. The research method used quasi-experimental with the pre test-post test design control group. As many as 70 elderly who experienced balance disruption in the Puskemas Sarijadi had participated in this study. The sample was taken by using consecutive sampling, then divided equally between the control group and the intervention group. The instrument used to measure elderly balance is Short Physical Performance Battery (SPPB). Data analysis is carried out in two stages, namely univariate analysis using the mean, standard deviation, maximum value and minimum value and percentage; then followed by bivariate analysis using the Wilcoxon signed rank test and independent T test. The result shows that there are differences in the balance of the elderly before and after the intervention in the treatment group, whereas in the control group there were no significant differences. Therefore, it can be concluded that Salsa dance can significantly improve elderly body balance, especially if done regularly. The increase will appear significant after the intervention carried out for 10 and 20 times. It is suggested that nurses utilize Salsa dance as an alternative in dealing with symptoms of body balance disorders in the elderly. This intervention can be implemented along with other activities of the elderly in community centre health services.
β-Glucan production of Saccharomyces cerevisiae in medium with different nitrogen sources in air-lift fermentor
Biodiversitas , 2007,
Abstract: β-Glucan is one of the most abundant polysaccharides in yeast Saccharomyces cerevisiae cell wall. The aim of this research is to explore an alternative nitrogen sources for β-glucan production. S. cerevisiae were grown in fermentation medium with different nitrogen sources. Peptone 2%, glutamic acid 0,5%, urea 0,2%, and diammonium hydrogen phosphate (DAHP) 0,02% were used for nitrogen source in the medium. A two liter air-lift fermentor was used in the fermentation process for 84 hours (T = 300C, pH 7, and 1.5 vvm for the aeration). During the fermentation, optical density, extraction of β-glucan, glucose and protein in hydrolisate cultured were determined. β-glucan production level is similar with the growth rate of yeast and followed by decreasing glucose and protein content in hydrolysis cultured. The highest and lowest β-glucan content were obtained from peptone (933.33 mg/L) and glutamic acid (633.33 mg/L) as a nitrogen source in cells cultured after fermentation completed respectively. Yeast cells cultured with urea and DAHP as a nitrogen source give the same content of β-glucan about 733.33 mg/L. β-glucan concentration produced in medium with urea was a higher than that produced using glutamic acid and DAHP as a nitrogen source. The result indicated that urea can be used as an alternative nitrogen source for the production of β-glucan. Urea is easily available and cheaper than peptone, glutamic acid and DAHP.
Production of Beta-1,3 Glucan from Agrobacterium and Its Wound Healing Activity on White Rat
Kusmiati,Amarila Malik,Sukma Nuswantara,Syafrida Siregar
Makara Seri Sains , 2006,
Abstract: The objective of this study was to determine the activity of beta-1,3 glucan product extracted from local Agrobacterium sp Bro 1.2.1, both wild-type and mutant-type, on opened-wound healing process. Beta-1,3 glucan product was extracted by precipitation, and the purification was carried out by column chromatography as KCl gradient fractions. In this study, white Sprague Dawley rats were employed, and have been treated for opened-wound condition. Seven groups were performed in this experiment, i.e. the negative control, the positive control employing povidone iodine, the two groups of two commercial beta-1,3 glucan with 0,02 mg/4 cm2 each, and the last three groups of beta-1,3 glucan as the test group with 0,02 mg/4 cm2, 0,10 mg/4 cm2 and 0,50 mg/4 cm2, respectively. The result showed significant differences of wound-healing activity performing statistical analysis of the least significance between the negative control, the positive control, as well as the highest dose of the test group of beta-1,3 glucan, at the dose of 0,5 mg/4 cm2 (p<0.05).
Beta Glucan Production from Two Strains of Agrobacterium sp in Medium Containing of Molases and Uracil Combine
Biodiversitas , 2007,
Abstract: Production of β-glucan by Agrobacterium sp is influenced by the composition of nutrition in the fermentation media. Molases has been used successfully by others in the fermentation media of S. cerevisiae to increase the yield of -glucan, and similarly, uracil has been used in the fermentation media of Agrobacterium sp to increase the yield of -glucan. Investigations to increase the yield of -glucan by two strains of Agrobacterium sp, i.e. A1.5 (reference) and B4.4 (local strain), have been carried out by addition of various combination of molases and uracil into fermentation media, i.e. 5%(v/v) molase-0,05%(b/v) uracil; 5% molase-0,025% uracil; 10% molase-0,05% uracil; and 10% molase-0,025% uracil. The β-1,3-glucan and β-1,2-glucan fractions were separated by extraction method. Beta-glucan concentration was determined as the glucose monomer using the phenol-sulphate spectrophotometric method at 490 nm. The protein content was determined by a modified Lowry-spectrophotometric method at 750 nm. The results showed that all combination of molases and uracil in the fermentation media of Agrobacterium sp A1.5 and B4.4 strains have increased both the dry-weight yield of β-glucan (crude) and the β glucan content, with the highest was in a medium containing 10% molases-0,025% uracil combination. In the above medium, the A1.5 strain produced the highest β-glucan (7,5%) with the lowest protein content ( 8,4%) in the β-1,3-glucan fraction, while the β-glucan content in the β-1,2-glucan fraction were all lower than in the control media, while the protein content were all higher than in the control media. In the above media, the B4.4 strain produced the highest β-glucan, 7,2% in the β-1,3-glucan fraction, and 13,1% in β-1,2-glucan fraction, while the lowest protein content ( 8,4%) was in the β-1,3-glucan fraction. In conclusion, fermentation media of Agrobacterium sp A1.5 strain or B4.4 strain containing molase and uracil combination have increased both the dry-weight yield of total β-glucan (crude) and the β-glucan content, while reduced the protein content. There is no clear FTIR spectrum difference between supposedly β-1,2-glucan fraction and β 1,3-glucan fraction.
Influence of Uracil in Fermentation Media on β-Glucan Production by Agrobacterium Radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1
Kusmiati,Salmah Muhamad,Sukma Nuswantara,Swasono R.Tamat
Makara Seri Sains , 2007,
Abstract: Optimum β-glucan production can be achieved by an optimum condition in the fermentation media. Uracil, as a precursor of UDP-glucose, may act as a glucose donor in the formation of polysaccharides such as β-glucan. It is expected that addition of certain quantity of uracil into the fermentation media in a suitable growth phase of Agrobacterium radiobacter A 1.5 and Agrobacterium sp. Bro 1.2.1, will significantly increase the β-glucan production. In this investigation, 0.025%; 0.05% or 0.1% of uracil were added into the fermentation media during the logarithmic phase (24 hour) or stationary phase (46 hour) of growth. The β-glucan product was evaluated from the β-glucan (crude) dry-weight and from the β-glucan content. Beta-glucan content was determined as glucose by the Hisamatsu-AOAC and HPLC methods. The highest β-glucan (crude) dry-weight produced by the A. 1.5 was in a medium containg 0.025% uracil (24 hour), whilst by the A. Bro 1.2.1 was in a medium containg 0.1% uracil (46 hour), both higher than control. The highest β-glucan content produced by the A. 1.5 (27.03%) was in a medium containg 0.025% uracil (46 hour), while control produced only 23.28%. The highest β-glucan content produced by the Bro 1.2.1 (29.34%) was in a medium containg 0.025% uracil (24 hour), while control produced only 28.75%. Two-way anova analysis showed that there were no significant influence difference (α = 0,05) from various concentration of uracil in either growth phases, to the yield of β-glucan (crude) dry-weight nor to the β-glucan equivalent glucose content.
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